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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-22194 | |||||||||
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Title | PmtCD in peptidisc | |||||||||
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Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 4.3 Å | |||||||||
![]() | Zeytuni N / Hu J / Worrall LJ / Strynadka NCJ | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Structural insight into the ATP-driven exporter of virulent peptide toxins. Authors: N Zeytuni / S W Dickey / J Hu / H T Chou / L J Worrall / J A N Alexander / M L Carlson / M Nosella / F Duong / Z Yu / M Otto / N C J Strynadka / ![]() ![]() Abstract: is a major human pathogen that has acquired alarming broad-spectrum antibiotic resistance. One group of secreted toxins with key roles during infection is the phenol-soluble modulins (PSMs). PSMs ... is a major human pathogen that has acquired alarming broad-spectrum antibiotic resistance. One group of secreted toxins with key roles during infection is the phenol-soluble modulins (PSMs). PSMs are amphipathic, membrane-destructive cytolytic peptides that are exported to the host-cell environment by a designated adenosine 5'-triphosphate (ATP)-binding cassette (ABC) transporter, the PSM transporter (PmtABCD). Here, we demonstrate that the minimal Pmt unit necessary for PSM export is PmtCD and provide its first atomic characterization by single-particle cryo-EM and x-ray crystallography. We have captured the transporter in the ATP-bound state at near atomic resolution, revealing a type II ABC exporter fold, with an additional cytosolic domain. Comparison to a lower-resolution nucleotide-free map displaying an "open" conformation and putative hydrophobic inner chamber of a size able to accommodate the binding of two PSM peptides provides mechanistic insight and sets the foundation for therapeutic design. | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 1.6 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 11.5 KB 11.5 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 5.5 KB | Display | ![]() |
Images | ![]() | 47.5 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Voxel size | X=Y=Z: 1.31 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire : PmtCD
Entire | Name: PmtCD |
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Components |
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-Supramolecule #1: PmtCD
Supramolecule | Name: PmtCD / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all / Details: PmtCD ABC exporter |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Experimental: 120 KDa |
-Macromolecule #1: PmtC
Macromolecule | Name: PmtC / type: protein_or_peptide / ID: 1 / Enantiomer: LEVO |
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Sequence | String: MKLEHITKKY GSNVVLNDID FDFGDSRIVG LIGKNGVGKT TVMKVMNGNI IKFDGKVDID NADNIGFLIE HPKLYDNKSG LYNLKLFAQV LGKGFDKAYT DKIIDAFGMR PYIKKKVKKY SMGMKQKLAI AVSLMNKPKF LILDEPTNGM DPDGSIDVLT TIKSLVNELD ...String: MKLEHITKKY GSNVVLNDID FDFGDSRIVG LIGKNGVGKT TVMKVMNGNI IKFDGKVDID NADNIGFLIE HPKLYDNKSG LYNLKLFAQV LGKGFDKAYT DKIIDAFGMR PYIKKKVKKY SMGMKQKLAI AVSLMNKPKF LILDEPTNGM DPDGSIDVLT TIKSLVNELD MRILISSHKL EDIELICDRA VFLRDGHFVQ DVNMEEGVAS DTTIVTVDHK DFDRTEKYLA EHFQLQNVDK ADGHLMINAQ KNYQVILKAL SELDIYPKYI ETRKSSLRDT YFNINQRGDK |
-Macromolecule #2: PmtD
Macromolecule | Name: PmtD / type: protein_or_peptide / ID: 2 / Enantiomer: LEVO |
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Sequence | String: MHHHHHHHHH HSSGLVPRGS HRILNLVKYD LYSIFKSPLT YLAILVVSSL IATQSILMAN SMDNPKHIIV YGSVFAAAKW LLLIIGLMFV VKTITRDFSQ GTIQLYMSKV KTRVGYIISK TISIILISIL FALIHYVILI VVQASSNGKN LAFSKYVDNL WFFLIFLLFF ...String: MHHHHHHHHH HSSGLVPRGS HRILNLVKYD LYSIFKSPLT YLAILVVSSL IATQSILMAN SMDNPKHIIV YGSVFAAAKW LLLIIGLMFV VKTITRDFSQ GTIQLYMSKV KTRVGYIISK TISIILISIL FALIHYVILI VVQASSNGKN LAFSKYVDNL WFFLIFLLFF GLFLFLITLA SQKTAMIFSL GVFLVLIVPF IKPFITFIPR YGEKVLDAFD YIPFAYLTDK MISSNFDFSN WQWVISLGSI VIFFILNILY VAKKDI |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 1 mg/mL | |||||||||
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Buffer | pH: 7.4 Component:
Details: Solution made fresh from concentrated stocks and filtered | |||||||||
Grid | Model: Quantifoil R1.2/1.3 / Support film - Material: CARBON / Support film - topology: HOLEY | |||||||||
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK I |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Average electron dose: 1.16 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
-Atomic model buiding 1
Refinement | Protocol: AB INITIO MODEL |
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