Biomedical Advanced Research and Development Authority (BARDA)
HHSO100201700020C
Citation
Journal: Science / Year: 2020 Title: Studies in humanized mice and convalescent humans yield a SARS-CoV-2 antibody cocktail. Authors: Johanna Hansen / Alina Baum / Kristen E Pascal / Vincenzo Russo / Stephanie Giordano / Elzbieta Wloga / Benjamin O Fulton / Ying Yan / Katrina Koon / Krunal Patel / Kyung Min Chung / Aynur ...Authors: Johanna Hansen / Alina Baum / Kristen E Pascal / Vincenzo Russo / Stephanie Giordano / Elzbieta Wloga / Benjamin O Fulton / Ying Yan / Katrina Koon / Krunal Patel / Kyung Min Chung / Aynur Hermann / Erica Ullman / Jonathan Cruz / Ashique Rafique / Tammy Huang / Jeanette Fairhurst / Christen Libertiny / Marine Malbec / Wen-Yi Lee / Richard Welsh / Glen Farr / Seth Pennington / Dipali Deshpande / Jemmie Cheng / Anke Watty / Pascal Bouffard / Robert Babb / Natasha Levenkova / Calvin Chen / Bojie Zhang / Annabel Romero Hernandez / Kei Saotome / Yi Zhou / Matthew Franklin / Sumathi Sivapalasingam / David Chien Lye / Stuart Weston / James Logue / Robert Haupt / Matthew Frieman / Gang Chen / William Olson / Andrew J Murphy / Neil Stahl / George D Yancopoulos / Christos A Kyratsous / Abstract: Neutralizing antibodies have become an important tool in treating infectious diseases. Recently, two separate approaches yielded successful antibody treatments for Ebola-one from genetically ...Neutralizing antibodies have become an important tool in treating infectious diseases. Recently, two separate approaches yielded successful antibody treatments for Ebola-one from genetically humanized mice and the other from a human survivor. Here, we describe parallel efforts using both humanized mice and convalescent patients to generate antibodies against the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein, which yielded a large collection of fully human antibodies that were characterized for binding, neutralization, and three-dimensional structure. On the basis of these criteria, we selected pairs of highly potent individual antibodies that simultaneously bind the receptor binding domain of the spike protein, thereby providing ideal partners for a therapeutic antibody cocktail that aims to decrease the potential for virus escape mutants that might arise in response to selective pressure from a single-antibody treatment.
History
Deposition
Jun 10, 2020
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Header (metadata) release
Jun 24, 2020
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Map release
Jun 24, 2020
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Update
Jan 27, 2021
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Current status
Jan 27, 2021
Processing site: RCSB / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
#259 - Jul 2021 Designed Proteins and Citizen Science similarity (1)
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Map
File
Download / File: emd_22137.map.gz / Format: CCP4 / Size: 83.7 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel size
X=Y=Z: 0.85 Å
Density
Contour Level
By AUTHOR: 0.1 / Movie #1: 0.1
Minimum - Maximum
-0.22156094 - 0.46713254
Average (Standard dev.)
0.0002829932 (±0.013483254)
Symmetry
Space group: 1
Details
EMDB XML:
Map geometry
Axis order
X
Y
Z
Origin
0
0
0
Dimensions
280
280
280
Spacing
280
280
280
Cell
A=B=C: 238.0 Å α=β=γ: 90.0 °
CCP4 map header:
mode
Image stored as Reals
Å/pix. X/Y/Z
0.85
0.85
0.85
M x/y/z
280
280
280
origin x/y/z
0.000
0.000
0.000
length x/y/z
238.000
238.000
238.000
α/β/γ
90.000
90.000
90.000
start NX/NY/NZ
43
43
33
NX/NY/NZ
116
118
137
MAP C/R/S
1
2
3
start NC/NR/NS
0
0
0
NC/NR/NS
280
280
280
D min/max/mean
-0.222
0.467
0.000
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Supplemental data
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Sample components
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Entire : Complex of SARS-CoV-2 spike protein receptor binding domain with ...
Entire
Name: Complex of SARS-CoV-2 spike protein receptor binding domain with Fab fragments of two neutralizing antibodies
Components
Complex: Complex of SARS-CoV-2 spike protein receptor binding domain with Fab fragments of two neutralizing antibodies
Complex: spike protein receptor binding domain
Protein or peptide: Spike protein S1
Complex: REGN10933 antibody Fab fragment
Protein or peptide: REGN10933 antibody Fab fragment light chain
Protein or peptide: REGN10933 antibody Fab fragment heavy chain
Complex: REGN10987 antibody Fab fragment
Protein or peptide: REGN10987 antibody Fab fragment heavy chain
Protein or peptide: REGN10987 antibody Fab fragment light chain
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Supramolecule #1: Complex of SARS-CoV-2 spike protein receptor binding domain with ...
Supramolecule
Name: Complex of SARS-CoV-2 spike protein receptor binding domain with Fab fragments of two neutralizing antibodies type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
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Supramolecule #2: spike protein receptor binding domain
In the structure databanks used in Yorodumi, some data are registered as the other names, "COVID-19 virus" and "2019-nCoV". Here are the details of the virus and the list of structure data.
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