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- PDB-4x04: CRYSTAL STRUCTURE OF A TRAP PERIPLASMIC SOLUTE BINDING PROTEIN FR... -

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Basic information

Entry
Database: PDB / ID: 4x04
TitleCRYSTAL STRUCTURE OF A TRAP PERIPLASMIC SOLUTE BINDING PROTEIN FROM CITROBACTER KOSERI (CKO_04899, TARGET EFI-510094) WITH BOUND D-glucuronate
Componentssolute binding protein
KeywordsSOLUTE BINDING PROTEIN / ENZYME FUNCTION INITIATIVE / EFI / Structural Genomics
Function / homology
Function and homology information


transmembrane transport / outer membrane-bounded periplasmic space / metal ion binding
Similarity search - Function
TRAP transporter solute receptor, DctP family / Bacterial extracellular solute-binding protein, family 7 / TRAP transporter solute receptor DctP / TRAP transporter solute receptor DctP superfamily / Bacterial extracellular solute-binding protein, family 7 / D-Maltodextrin-Binding Protein; domain 2 / 3-Layer(aba) Sandwich / Alpha Beta
Similarity search - Domain/homology
beta-D-glucopyranuronic acid / 2,3-diketo-L-gulonate TRAP transporter substrate-binding periplasmic protein
Similarity search - Component
Biological speciesCitrobacter koseri (bacteria)
MethodX-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.5 Å
AuthorsYadava, U. / Vetting, M.W. / Al Obaidi, N.F. / Toro, R. / Morisco, L.L. / Benach, J. / Wasserman, S.R. / Attonito, J.D. / Scott Glenn, A. / Chamala, S. ...Yadava, U. / Vetting, M.W. / Al Obaidi, N.F. / Toro, R. / Morisco, L.L. / Benach, J. / Wasserman, S.R. / Attonito, J.D. / Scott Glenn, A. / Chamala, S. / Chowdhury, S. / Lafleur, J. / Love, J. / Seidel, R.D. / Whalen, K.L. / Gerlt, J.A. / Almo, S.C. / Enzyme Function Initiative (EFI)
CitationJournal: To be published
Title: CRYSTAL STRUCTURE OF A TRAP PERIPLASMIC SOLUTE BINDING PROTEIN FROM CITROBACTER KOSERI (CKO_04899, TARGET EFI-510094) WITH BOUND D-glucuronate
Authors: Yadava, U. / Vetting, M.W. / Al Obaidi, N.F. / Toro, R. / Morisco, L.L. / Benach, J. / Wasserman, S.R. / Attonito, J.D. / Scott Glenn, A. / Chamala, S. / Chowdhury, S. / Lafleur, J. / Love, ...Authors: Yadava, U. / Vetting, M.W. / Al Obaidi, N.F. / Toro, R. / Morisco, L.L. / Benach, J. / Wasserman, S.R. / Attonito, J.D. / Scott Glenn, A. / Chamala, S. / Chowdhury, S. / Lafleur, J. / Love, J. / Seidel, R.D. / Whalen, K.L. / Gerlt, J.A. / Almo, S.C. / Enzyme Function Initiative (EFI)
History
DepositionNov 20, 2014Deposition site: RCSB / Processing site: RCSB
Revision 1.0Dec 10, 2014Provider: repository / Type: Initial release
Revision 1.1Sep 27, 2017Group: Derived calculations / Refinement description ...Derived calculations / Refinement description / Source and taxonomy / Structure summary
Category: entity / entity_src_gen ...entity / entity_src_gen / pdbx_struct_oper_list / software
Item: _entity.pdbx_description / _entity_src_gen.pdbx_alt_source_flag ..._entity.pdbx_description / _entity_src_gen.pdbx_alt_source_flag / _pdbx_struct_oper_list.symmetry_operation / _software.classification
Revision 1.2Jul 29, 2020Group: Data collection / Derived calculations / Structure summary
Category: chem_comp / entity ...chem_comp / entity / pdbx_chem_comp_identifier / pdbx_entity_nonpoly / pdbx_struct_conn_angle / struct_conn / struct_site / struct_site_gen
Item: _chem_comp.name / _chem_comp.type ..._chem_comp.name / _chem_comp.type / _entity.pdbx_description / _pdbx_entity_nonpoly.name / _pdbx_struct_conn_angle.ptnr1_auth_asym_id / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr1_symmetry / _pdbx_struct_conn_angle.ptnr3_auth_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.ptnr3_symmetry / _pdbx_struct_conn_angle.value / _struct_conn.conn_type_id / _struct_conn.id / _struct_conn.pdbx_dist_value / _struct_conn.pdbx_leaving_atom_flag / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id / _struct_conn.ptnr2_symmetry
Description: Carbohydrate remediation / Provider: repository / Type: Remediation
Revision 1.3Sep 27, 2023Group: Data collection / Database references ...Data collection / Database references / Refinement description / Structure summary
Category: chem_comp / chem_comp_atom ...chem_comp / chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _chem_comp.pdbx_synonyms / _database_2.pdbx_DOI / _database_2.pdbx_database_accession
Revision 1.4Nov 15, 2023Group: Data collection / Category: chem_comp_atom / chem_comp_bond / Item: _chem_comp_atom.atom_id / _chem_comp_bond.atom_id_2
Revision 1.5Oct 23, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: solute binding protein
B: solute binding protein
C: solute binding protein
D: solute binding protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)148,13216
Polymers147,0944
Non-polymers1,03812
Water18,3751020
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)175.000, 175.000, 125.640
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number92
Space group name H-MP41212
Components on special symmetry positions
IDModelComponents
11C-404-

MG

21C-513-

HOH

31C-515-

HOH

41C-525-

HOH

Detailsbiological unit is a monomer

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Components

#1: Protein
solute binding protein


Mass: 36773.602 Da / Num. of mol.: 4 / Fragment: solute binding protein
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Citrobacter koseri (bacteria) / Strain: ATCC BAA-895 / CDC 4225-83 / SGSC4696 / Gene: CKO_04899 / Plasmid: pET / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: A8AR30
#2: Sugar
ChemComp-BDP / beta-D-glucopyranuronic acid / beta-D-glucuronic acid / D-glucuronic acid / glucuronic acid


Type: D-saccharide, beta linking / Mass: 194.139 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C6H10O7
IdentifierTypeProgram
DGlcpAbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
b-D-glucopyranuronic acidCOMMON NAMEGMML 1.0
b-D-GlcpAIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcASNFG CARBOHYDRATE SYMBOLGMML 1.0
#3: Chemical
ChemComp-CL / CHLORIDE ION


Mass: 35.453 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: Cl
#4: Chemical ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Mg
#5: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 1020 / Source method: isolated from a natural source / Formula: H2O
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.27 Å3/Da / Density % sol: 62.38 %
Crystal growTemperature: 298 K / Method: vapor diffusion, sitting drop / pH: 8.5
Details: Protein (43.2 mg/ml, 10 mM HEPES pH 7.5, 5 mM DTT, 5% Glycerol, 10 mM D-glucuronate); Reservoir (0.2 M Magnesium Chloride, 20 %(w/v) PEG 3350, 0.1M Tris pH 8.5); Cryoprotection (80%(50%) PEG 3350,20% Reservoir)

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: ROTATING ANODE / Type: RIGAKU RU300 / Wavelength: 1.54056 Å
DetectorType: RIGAKU RAXIS IV / Detector: IMAGE PLATE / Date: Nov 12, 2014 / Details: MIRRORS
RadiationMonochromator: GRAPHITE / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.54056 Å / Relative weight: 1
ReflectionResolution: 2.5→50 Å / Num. obs: 94018 / % possible obs: 99.8 % / Redundancy: 11.9 % / Biso Wilson estimate: 14.42 Å2 / Rmerge(I) obs: 0.102 / Χ2: 0.97 / Net I/av σ(I): 24.586 / Net I/σ(I): 9.2 / Num. measured all: 803840
Reflection shell

Diffraction-ID: 1 / Rejects: _

Resolution (Å)Redundancy (%)Rmerge(I) obsNum. unique allΧ2% possible all
2.5-2.549.60.40132970.96799.5
2.54-2.599.70.36233140.9799.6
2.59-2.649.70.34533400.9699.7
2.64-2.699.80.29933580.98399.7
2.69-2.75100.2733110.99799.7
2.75-2.8210.10.24333331.01699.7
2.82-2.8910.40.23633481.01199.8
2.89-2.9610.70.20933501.03799.9
2.96-3.05110.18533451.039100
3.05-3.1511.40.15833751.041100
3.15-3.2611.80.14133781.039100
3.26-3.3912.30.1233621.032100
3.39-3.5512.80.09733701.034100
3.55-3.7313.20.08933901.082100
3.73-3.9713.80.07534030.968100
3.97-4.2714.30.05834020.847100
4.27-4.714.50.0534290.799100
4.7-5.3814.50.05234460.804100
5.38-6.7814.40.06334920.871100
6.78-5013.10.05236451.03899

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Processing

Software
NameVersionClassification
HKL-3000data reduction
PHENIXrefinement
PDB_EXTRACT3.15data extraction
MOSFLMdata reduction
PHENIXphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB entry 4N8Y

4n8y


Resolution: 2.5→40.73 Å / SU ML: 0.23 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 18.34 / Stereochemistry target values: ML
RfactorNum. reflection% reflectionSelection details
Rfree0.2048 4665 4.96 %Random
Rwork0.1538 89353 --
obs0.1564 94018 95.13 %-
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 83.46 Å2 / Biso mean: 18.2322 Å2 / Biso min: 0.95 Å2
Refinement stepCycle: final / Resolution: 2.5→40.73 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms9372 0 60 1020 10452
Biso mean--12.06 23.53 -
Num. residues----1204
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0089608
X-RAY DIFFRACTIONf_angle_d1.10912984
X-RAY DIFFRACTIONf_chiral_restr0.0421448
X-RAY DIFFRACTIONf_plane_restr0.0061688
X-RAY DIFFRACTIONf_dihedral_angle_d14.3493616
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 30

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
2.2-2.2250.2689950.22451995209065
2.225-2.25120.26971100.2242139224969
2.2512-2.27870.27271250.2142340246575
2.2787-2.30750.25581340.20452525265982
2.3075-2.33790.25881360.19982700283687
2.3379-2.36990.24791400.19542863300392
2.3699-2.40370.25461410.18052971311296
2.4037-2.43960.24241280.18042978310696
2.4396-2.47770.22651690.18183048321797
2.4777-2.51830.22981550.18843006316198
2.5183-2.56180.24191670.17893064323199
2.5618-2.60830.22881720.16613083325599
2.6083-2.65850.23541700.16453077324799
2.6585-2.71270.21091590.156230893248100
2.7127-2.77170.2271740.151430913265100
2.7717-2.83620.2051690.162631173286100
2.8362-2.90710.2171460.159731203266100
2.9071-2.98570.24311680.162431063274100
2.9857-3.07350.21691700.165731003270100
3.0735-3.17270.21051730.156331223295100
3.1727-3.2860.21591700.151631113281100
3.286-3.41750.17021550.150131473302100
3.4175-3.5730.19231780.140731433321100
3.573-3.76120.19941780.137631043282100
3.7612-3.99670.16811730.125731633336100
3.9967-4.30490.1581520.113531723324100
4.3049-4.73760.13661480.107331893337100
4.7376-5.42170.1571640.115132103374100
5.4217-6.82560.17311610.136832533414100
6.8256-40.73690.17911850.1483327351298
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.0091-0.0014-0.00050.0085-0.00080.00070.02870.00230.0267-0.00640.0101-0.017-0.01890.00260.03590.02840.00810.09350.0421-0.00050.0321212.1269142.1547-10.1727
20.00050-0.00050.00040.00070.001-0.0117-0.00220.00120.01140.0039-0.0158-0.0077-0.002900.06710.02260.00140.0979-0.01160.0468209.2444130.84471.6768
30.0028-0.00380.00150.0134-0.00420.00360.0087-0.01630.0105-0.0031-0.0225-0.0111-0.0104-0.0008-0.0098-0.03410.0379-0.00520.02-0.03330.0678193.9428139.27621.1341
40.0008-0.0010.00190.00110.00240.00540.0001-0.01040.01040.0086-0.00850.00770.00340.0042-0.00640.03490.0020.00570.0463-0.05380.063200.3308145.42046.2095
50.0010.0004-0.00210.0031-0.00180.0028-0.0111-0.0005-0.0176-0.0045-0.02260.01190.0168-0.0114-0.00080.04230.01340.0090.084-0.0530.1088184.8414121.7296-6.7565
60.0030.0023-0.00020.00140.00230.00210.0016-0.0098-0.01630.00660.0110.00390.0041-0.00180.01550.00710.02950.02820.03620.01270.0691190.0209121.19433.2944
70.0124-0.0067-0.0090.00640.00340.00530.0178-0.0327-0.04790.00230.0105-0.00940.00470.03900.05150.0054-0.00080.0829-0.00710.0933198.4175128.2441-5.4625
80.015-0.0064-0.01380.0091-0.00180.01380.02470.0272-0.007-0.04270.01560.0307-0.0476-0.02180.0010.0742-0.00180.01150.0772-0.01340.0899193.2401136.9003-10.3336
90.0067-0.0025-0.00390.0081-0.00610.011-0.0003-0.0140.0080.0046-0.00450.0023-0.00530.0047-0.0040.05430.028-0.00290.0972-0.04990.1014182.7381140.19728.9785
100.0015-0.0008-0.00040.00990.00540.00220.01510.0095-0.0221-0.01020.0045-0.01730.01110.01760.02230.0846-0.00590.00160.1116-0.1135-0.0014226.4632137.761521.3712
110.0007-0.0007-0.00080.00150.00070.0010.0096-0.0055-0.0025-0.00140.0014-0.0006-0.00670.0030.0040.0588-0.0408-0.00250.0283-0.00160.0324214.8488142.860131.8175
120.0362-0.00740.01160.016-0.00220.0021-0.04530.02880.05050.01060.0129-0.0354-0.05280.0685-0.0110.0292-0.13130.0095-0.04460.03270.0326226.9294154.529333.0903
130.0158-0.00030.00750.03220.0110.0162-0.0069-0.00040.01330.0026-0.03070.0252-0.0543-0.0089-0.00370.1818-0.0098-0.0493-0.0119-0.08460.0058209.1216165.845726.3745
140.0170.0190.03230.06080.06440.0965-0.08250.03090.019-0.0734-0.02340.0557-0.07180.0646-0.17350.0484-0.0541-0.0753-0.0162-0.0887-0.0863217.9408155.578622.1806
150.00740.001-0.00420.00580.0020.0056-0.0115-0.00450.00810.0025-0.00460.0026-0.01920.0027-0.00490.1721-0.088-0.04290.0866-0.03550.1294226.715168.336938.3276
160.0019-0.00060.00050.00180.00070.00090.00220.0047-0.0062-0.00620.01220.01110.0141-0.0075-00.0634-0.0022-0.02690.0936-0.00130.0872138.9492121.5849-9.6682
170.0038-0.00080.00020.0002-0.00040.0068-0.00770.00310.0046-0.0011-0.0091-0.00090.0027-0.0141-0.0140.0241-0.0176-0.02780.0486-0.0010.0802134.0369123.8332-6.514
180.00120.00040.00110.0010.00080.0011-0.00720.00670.0017-0.0217-0.0211-0.0245-0.00720.0344-0.01880.00920.00490.00240.0458-0.00540.0413149.9099127.98733.9915
190.00460.00490.00340.01440.00760.00480.0193-0.0047-0.0155-0.00820.0126-0.02220.01770.00020.01770.02130.0074-0.00020.05640.01440.0571156.7406116.73492.1731
200.0067-0.00010.00710.0009-0.00270.00420.00290.00750.02220.0097-0.0099-0.04020.01760.0124-00.0575-0.0079-0.0010.08320.0090.1014156.7187132.9103-1.6908
210.0018-0.001-0.00270.0024-0.00080.0073-0.0134-0.01630.00550.0086-0.0018-0.00660.00160.002-0.01160.00850.0312-0.02140.0675-0.02940.0691160.3357141.67152.75
220.0118-0.0085-0.00110.01210.0056-0.00030.0213-0.01550.0466-0.00040.01610.03590.0156-0.0249-00.0432-0.0030.00130.08520.00240.0865151.3058135.8838-4.7937
230.00850.00160.0107-0.00030.00080.0099-0.00470.0471-0.011-0.0156-0.006-0.00090.00980.035400.0681-0.00410.00380.1072-0.00080.0651149.6785120.2293-10.9936
240.00880.0032-0.00140.0049-0.00060.0024-0.0091-0.00120.0083-0.00270.0072-0.00370.0003-0.0044-0.00290.01570.01540.00240.0770.01980.0677168.6139135.8125-1.4224
250.0016-0.00010.00080.00070.0010.00160.005-0.00410.00050.005-0.01-0.0030.00080.004100.0620.0193-0.00060.1070.00880.1582166.7465119.44159.0175
260.00490.00080.00320.00390.00080.00590.0181-0.01060.01260.01120.0238-0.0228-0.01080.0360.03220.112-0.03890.00610.1331-0.08790.0334228.4962127.09199.0934
270.0043-0.0001-0.00180.00070.00090.00230.0271-0.00030.0088-0.0113-0.01140.00440.010.028400.06880.0207-0.01020.05920.01020.0689219.8643118.3068-2.9971
280.0146-0.00040.01310.0005-0.00060.01030.0132-0.0156-0.0321-0.00170.019-0.01260.02270.02670.03790.01970.0866-0.03030.00010.0782-0.0218229.4103108.3986-2.0464
290.0081-0.00960.00530.0145-0.00260.0082-0.0026-0.003-0.0148-0.00050.00440.00880.01760.0004-0.00040.10010.01830.01020.0677-0.0030.0832208.092399.46988.9005
300.00140.0028-0.00030.0053-0.00060.0020.01690.0066-0.0073-0.00210.00130.01750.0182-0.00060.0040.11030.0441-0.05970.00760.0120.0679206.8429104.1407-1.2798
310.001-0.0023-0.00450.0120.00840.00670.0237-0.02020.02440.00890.00880.03750.01240.026600.07580.0043-0.00380.07330.0040.0787214.2973112.99646.3671
32-0.00060.0028-0.00410.0023-0.00410.00820.00830.0023-0.00920.02940.0152-0.00510.01770.03760.02760.07660.0438-0.01490.04920.0568-0.0379228.8736114.50111.7642
33-0.0008-0.0005-0.00330.01820.00070.00360.0344-0.0078-0.0161-0.01930.00630.0180.0176-0.00460.01290.17780.0239-0.04510.01140.01180.0868213.597494.99363.2404
340.00050.00030.00080.00130.00010.0007-0.01430.0029-0.0033-0.009-0.00870.0096-0.0082-0.0035-00.23470.0879-0.01620.16270.00050.1907229.89796.5613-7.6817
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 27 through 66 )A0
2X-RAY DIFFRACTION2chain 'A' and (resid 67 through 83 )A0
3X-RAY DIFFRACTION3chain 'A' and (resid 84 through 124 )A0
4X-RAY DIFFRACTION4chain 'A' and (resid 125 through 148 )A0
5X-RAY DIFFRACTION5chain 'A' and (resid 149 through 167 )A0
6X-RAY DIFFRACTION6chain 'A' and (resid 168 through 195 )A0
7X-RAY DIFFRACTION7chain 'A' and (resid 196 through 253 )A0
8X-RAY DIFFRACTION8chain 'A' and (resid 254 through 305 )A0
9X-RAY DIFFRACTION9chain 'A' and (resid 306 through 327 )A0
10X-RAY DIFFRACTION10chain 'B' and (resid 27 through 66 )B0
11X-RAY DIFFRACTION11chain 'B' and (resid 67 through 83 )B0
12X-RAY DIFFRACTION12chain 'B' and (resid 84 through 148 )B0
13X-RAY DIFFRACTION13chain 'B' and (resid 149 through 187 )B0
14X-RAY DIFFRACTION14chain 'B' and (resid 188 through 305 )B0
15X-RAY DIFFRACTION15chain 'B' and (resid 306 through 327 )B0
16X-RAY DIFFRACTION16chain 'C' and (resid 27 through 56 )C0
17X-RAY DIFFRACTION17chain 'C' and (resid 57 through 73 )C0
18X-RAY DIFFRACTION18chain 'C' and (resid 74 through 114 )C0
19X-RAY DIFFRACTION19chain 'C' and (resid 115 through 136 )C0
20X-RAY DIFFRACTION20chain 'C' and (resid 137 through 167 )C0
21X-RAY DIFFRACTION21chain 'C' and (resid 168 through 187 )C0
22X-RAY DIFFRACTION22chain 'C' and (resid 188 through 253 )C0
23X-RAY DIFFRACTION23chain 'C' and (resid 254 through 288 )C0
24X-RAY DIFFRACTION24chain 'C' and (resid 289 through 313 )C0
25X-RAY DIFFRACTION25chain 'C' and (resid 314 through 327 )C0
26X-RAY DIFFRACTION26chain 'D' and (resid 27 through 66 )D0
27X-RAY DIFFRACTION27chain 'D' and (resid 67 through 107 )D0
28X-RAY DIFFRACTION28chain 'D' and (resid 108 through 148 )D0
29X-RAY DIFFRACTION29chain 'D' and (resid 149 through 167 )D0
30X-RAY DIFFRACTION30chain 'D' and (resid 168 through 195 )D0
31X-RAY DIFFRACTION31chain 'D' and (resid 196 through 253 )D0
32X-RAY DIFFRACTION32chain 'D' and (resid 254 through 288 )D0
33X-RAY DIFFRACTION33chain 'D' and (resid 289 through 313 )D0
34X-RAY DIFFRACTION34chain 'D' and (resid 314 through 327 )D0

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