- EMDB-21922: Low curvature lateral interaction within a 13-protofilament, Taxo... -
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基本情報
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データベース: EMDB / ID: EMD-21922
タイトル
Low curvature lateral interaction within a 13-protofilament, Taxol stabilized microtubule
マップデータ
試料
細胞器官・細胞要素: Microtubules stabilized with Taxol in the presence of a low concentration of Kinesin Dimer and ADP
タンパク質・ペプチド: Tubulin alpha-1B chain
タンパク質・ペプチド: Tubulin beta chain
リガンド: GUANOSINE-5'-TRIPHOSPHATE
リガンド: MAGNESIUM ION
リガンド: GUANOSINE-5'-DIPHOSPHATE
リガンド: TAXOL
キーワード
Microtubule / STRUCTURAL PROTEIN
機能・相同性
機能・相同性情報
Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane / Cilium Assembly / Intraflagellar transport / Carboxyterminal post-translational modifications of tubulin / Sealing of the nuclear envelope (NE) by ESCRT-III / Kinesins / Resolution of Sister Chromatid Cohesion / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / COPI-dependent Golgi-to-ER retrograde traffic ...Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane / Cilium Assembly / Intraflagellar transport / Carboxyterminal post-translational modifications of tubulin / Sealing of the nuclear envelope (NE) by ESCRT-III / Kinesins / Resolution of Sister Chromatid Cohesion / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / COPI-dependent Golgi-to-ER retrograde traffic / RHO GTPases activate IQGAPs / COPI-independent Golgi-to-ER retrograde traffic / COPI-mediated anterograde transport / RHO GTPases Activate Formins / MHC class II antigen presentation / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / Aggrephagy / The role of GTSE1 in G2/M progression after G2 checkpoint / Separation of Sister Chromatids / Recruitment of NuMA to mitotic centrosomes / Hedgehog 'off' state / positive regulation of axon guidance / intercellular bridge / microtubule-based process / 加水分解酵素; 酸無水物に作用; GTPに作用・細胞または細胞小器官の運動に関与 / structural constituent of cytoskeleton / mitotic spindle / cerebral cortex development / microtubule cytoskeleton organization / microtubule cytoskeleton / nervous system development / mitotic cell cycle / microtubule / protein heterodimerization activity / GTPase activity / GTP binding / metal ion binding / cytoplasm / cytosol 類似検索 - 分子機能
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
米国
引用
ジャーナル: Proc Natl Acad Sci U S A / 年: 2020 タイトル: Dynamic and asymmetric fluctuations in the microtubule wall captured by high-resolution cryoelectron microscopy. 著者: Garrett E Debs / Michael Cha / Xueqi Liu / Andrew R Huehn / Charles V Sindelar / 要旨: Microtubules are tubular polymers with essential roles in numerous cellular activities. Structures of microtubules have been captured at increasing resolution by cryo-EM. However, dynamic properties ...Microtubules are tubular polymers with essential roles in numerous cellular activities. Structures of microtubules have been captured at increasing resolution by cryo-EM. However, dynamic properties of the microtubule are key to its function, and this behavior has proved difficult to characterize at a structural level due to limitations in existing structure determination methods. We developed a high-resolution cryo-EM refinement method that divides an imaged microtubule into its constituent protofilaments, enabling deviations from helicity and other sources of heterogeneity to be quantified and corrected for at the single-subunit level. We demonstrate that this method improves the resolution of microtubule 3D reconstructions and substantially reduces anisotropic blurring artifacts, compared with methods that utilize helical symmetry averaging. Moreover, we identified an unexpected, discrete behavior of the m-loop, which mediates lateral interactions between neighboring protofilaments and acts as a flexible hinge between them. The hinge angle adopts preferred values corresponding to distinct conformations of the m-loop that are incompatible with helical symmetry. These hinge angles fluctuate in a stochastic manner, and perfectly cylindrical microtubule conformations are thus energetically and entropically penalized. The hinge angle can diverge further from helical symmetry at the microtubule seam, generating a subpopulation of highly distorted microtubules. However, the seam-distorted subpopulation disappears in the presence of Taxol, a microtubule stabilizing agent. These observations provide clues into the structural origins of microtubule flexibility and dynamics and highlight the role of structural polymorphism in defining microtubule behavior.
全体 : Microtubules stabilized with Taxol in the presence of a low conce...
全体
名称: Microtubules stabilized with Taxol in the presence of a low concentration of Kinesin Dimer and ADP
要素
細胞器官・細胞要素: Microtubules stabilized with Taxol in the presence of a low concentration of Kinesin Dimer and ADP
タンパク質・ペプチド: Tubulin alpha-1B chain
タンパク質・ペプチド: Tubulin beta chain
リガンド: GUANOSINE-5'-TRIPHOSPHATE
リガンド: MAGNESIUM ION
リガンド: GUANOSINE-5'-DIPHOSPHATE
リガンド: TAXOL
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超分子 #1: Microtubules stabilized with Taxol in the presence of a low conce...
超分子
名称: Microtubules stabilized with Taxol in the presence of a low concentration of Kinesin Dimer and ADP タイプ: organelle_or_cellular_component / ID: 1 / 親要素: 0 / 含まれる分子: #1-#2