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- EMDB-21320: Trimeric Photosystem I from the High-Light Tolerant Cyanobacteria... -

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Basic information

Entry
Database: EMDB / ID: EMD-21320
TitleTrimeric Photosystem I from the High-Light Tolerant Cyanobacteria Cyanobacterium Aponinum
Map data
Sample
  • Complex: Trimeric PSI from a High Light tolerant cyanobacteria, Cyanobacterium aponinum
    • Protein or peptide: x 11 types
  • Ligand: x 8 types
Function / homology
Function and homology information


photosystem I reaction center / photosystem I / photosynthetic electron transport in photosystem I / : / photosystem I / chlorophyll binding / plasma membrane-derived thylakoid membrane / photosynthesis / 4 iron, 4 sulfur cluster binding / electron transfer activity ...photosystem I reaction center / photosystem I / photosynthetic electron transport in photosystem I / : / photosystem I / chlorophyll binding / plasma membrane-derived thylakoid membrane / photosynthesis / 4 iron, 4 sulfur cluster binding / electron transfer activity / membrane => GO:0016020 / magnesium ion binding / metal ion binding
Similarity search - Function
Photosystem I reaction center subunit PsaK / Photosystem I reaction centre subunit PsaK / Photosystem I PsaM, reaction centre superfamily / Photosystem I PsaM, reaction centre / Photosystem I protein M (PsaM) / Photosystem I reaction centre subunit PsaK superfamily / Photosystem I psaG and psaK proteins signature. / Photosystem I reaction center subunit V/PsaK / Photosystem I psaG / psaK / Photosystem I PsaL, reaction centre subunit XI ...Photosystem I reaction center subunit PsaK / Photosystem I reaction centre subunit PsaK / Photosystem I PsaM, reaction centre superfamily / Photosystem I PsaM, reaction centre / Photosystem I protein M (PsaM) / Photosystem I reaction centre subunit PsaK superfamily / Photosystem I psaG and psaK proteins signature. / Photosystem I reaction center subunit V/PsaK / Photosystem I psaG / psaK / Photosystem I PsaL, reaction centre subunit XI / Photosystem I, reaction centre subunit XI / Photosystem I PsaL, reaction centre subunit XI superfamily / Photosystem I reaction centre subunit XI / Photosystem I reaction centre subunit VIII / Photosystem I reaction centre subunit VIII / Photosystem I reaction centre subunit VIII superfamily / Photosystem I PsaF, reaction centre subunit III / Photosystem I PsaF, reaction centre subunit III superfamily / Photosystem I reaction centre subunit III / Photosystem I PsaJ, reaction centre subunit IX / Photosystem I PsaD / Photosystem I PsaJ, reaction centre subunit IX superfamily / Photosystem I, reaction centre subunit PsaD superfamily / Photosystem I reaction centre subunit IX / PsaJ / PsaD / Photosystem I PsaE, reaction centre subunit IV / Photosystem I reaction centre subunit IV / PsaE / Photosystem I protein PsaC / Photosystem I PsaA / Photosystem I PsaB / Photosystem I PsaA/PsaB, conserved site / Photosystem I psaA and psaB proteins signature. / Photosystem I PsaA/PsaB / Photosystem I PsaA/PsaB superfamily / Photosystem I psaA/psaB protein / Electron transport accessory-like domain superfamily / 4Fe-4S dicluster domain / 4Fe-4S ferredoxin, iron-sulphur binding, conserved site / 4Fe-4S ferredoxin-type iron-sulfur binding region signature. / 4Fe-4S ferredoxin-type iron-sulfur binding domain profile. / 4Fe-4S ferredoxin-type, iron-sulphur binding domain
Similarity search - Domain/homology
Photosystem I reaction center subunit PsaK / Photosystem I reaction center subunit XI / Photosystem I P700 chlorophyll a apoprotein A1 / Photosystem I reaction center subunit XII / Photosystem I reaction center subunit II / Photosystem I iron-sulfur center / Photosystem I reaction center subunit IV / PSI-F / Photosystem I reaction center subunit IX / Photosystem I P700 chlorophyll a apoprotein A2 / Photosystem I reaction center subunit VIII
Similarity search - Component
Biological speciesCyanobacterium aponinum 0216 (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.7 Å
AuthorsDobson Z / Toporik H / Vaughn N / Lin S / Williams D / Fromme P / Mazor Y
CitationJournal: Elife / Year: 2021
Title: The structure of photosystem I from a high-light-tolerant cyanobacteria.
Authors: Zachary Dobson / Safa Ahad / Jackson Vanlandingham / Hila Toporik / Natalie Vaughn / Michael Vaughn / Dewight Williams / Michael Reppert / Petra Fromme / Yuval Mazor /
Abstract: Photosynthetic organisms have adapted to survive a myriad of extreme environments from the earth's deserts to its poles, yet the proteins that carry out the light reactions of photosynthesis are ...Photosynthetic organisms have adapted to survive a myriad of extreme environments from the earth's deserts to its poles, yet the proteins that carry out the light reactions of photosynthesis are highly conserved from the cyanobacteria to modern day crops. To investigate adaptations of the photosynthetic machinery in cyanobacteria to excessive light stress, we isolated a new strain of cyanobacteria, 0216, from the extreme light environment of the Sonoran Desert. Here we report the biochemical characterization and the 2.7 Å resolution structure of trimeric photosystem I from this high-light-tolerant cyanobacterium. The structure shows a new conformation of the PsaL C-terminus that supports trimer formation of cyanobacterial photosystem I. The spectroscopic analysis of this photosystem I revealed a decrease in far-red absorption, which is attributed to a decrease in the number of long- wavelength chlorophylls. Using these findings, we constructed two chimeric PSIs in sp. PCC 6803 demonstrating how unique structural features in photosynthetic complexes can change spectroscopic properties, allowing organisms to thrive under different environmental stresses.
History
DepositionFeb 4, 2020-
Header (metadata) releaseMar 4, 2020-
Map releaseAug 4, 2021-
UpdateSep 8, 2021-
Current statusSep 8, 2021Processing site: RCSB / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 3
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 3
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6vpv
  • Surface level: 3
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_21320.png

Downloads & links

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Map

FileDownload / File: emd_21320.map.gz / Format: CCP4 / Size: 103 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.05067 Å
Density
Contour LevelBy AUTHOR: 2.1 / Movie #1: 3
Minimum - Maximum-24.049486 - 37.812508
Average (Standard dev.)0.0009920704 (±1.1479613)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderZYX
Origin000
Dimensions300300300
Spacing300300300
CellA=B=C: 315.19998 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.05066666666671.05066666666671.0506666666667
M x/y/z300300300
origin x/y/z0.0000.0000.000
length x/y/z315.200315.200315.200
α/β/γ90.00090.00090.000
start NX/NY/NZ000
NX/NY/NZ300300300
MAP C/R/S321
start NC/NR/NS000
NC/NR/NS300300300
D min/max/mean-24.04937.8130.001

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Supplemental data

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Sample components

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Entire : Trimeric PSI from a High Light tolerant cyanobacteria, Cyanobacte...

EntireName: Trimeric PSI from a High Light tolerant cyanobacteria, Cyanobacterium aponinum
Components
  • Complex: Trimeric PSI from a High Light tolerant cyanobacteria, Cyanobacterium aponinum
    • Protein or peptide: Photosystem I P700 chlorophyll a apoprotein A1
    • Protein or peptide: Photosystem I P700 chlorophyll a apoprotein A2
    • Protein or peptide: Photosystem I iron-sulfur center
    • Protein or peptide: Photosystem I reaction center subunit II
    • Protein or peptide: Photosystem I reaction center subunit IV
    • Protein or peptide: PSI-F
    • Protein or peptide: Photosystem I reaction center subunit VIII
    • Protein or peptide: Photosystem I reaction center subunit IX
    • Protein or peptide: Photosystem I reaction center subunit PsaK
    • Protein or peptide: Photosystem I reaction center subunit XI
    • Protein or peptide: Photosystem I reaction center subunit XII
  • Ligand: CHLOROPHYLL A ISOMER
  • Ligand: CHLOROPHYLL A
  • Ligand: PHYLLOQUINONEPhytomenadione
  • Ligand: IRON/SULFUR CLUSTERIron–sulfur cluster
  • Ligand: BETA-CAROTENEΒ-Carotene
  • Ligand: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE
  • Ligand: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE
  • Ligand: 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL

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Supramolecule #1: Trimeric PSI from a High Light tolerant cyanobacteria, Cyanobacte...

SupramoleculeName: Trimeric PSI from a High Light tolerant cyanobacteria, Cyanobacterium aponinum
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#11
Source (natural)Organism: Cyanobacterium aponinum 0216 (bacteria) / Strain: 216

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Macromolecule #1: Photosystem I P700 chlorophyll a apoprotein A1

MacromoleculeName: Photosystem I P700 chlorophyll a apoprotein A1 / type: protein_or_peptide / ID: 1 / Number of copies: 3 / Enantiomer: LEVO / EC number: photosystem I
Source (natural)Organism: Cyanobacterium aponinum 0216 (bacteria) / Strain: 216
Molecular weightTheoretical: 81.754039 KDa
SequenceString: KAKVIVDKDP VPTSFEKWGK PGHFDRTLAR GPKTTTWIWN LHADAHDFDS QTSDLEDISR KIFSAHFGHL AVVFVWLSGM YFHGAKFSN YSAWLADPLN IKPSAQVVWP VVGQDILNAD VGGGFHGIQI TSGFFQLWRA SGITNEYQLY CTAIGGLVMA G LMLFAGWF ...String:
KAKVIVDKDP VPTSFEKWGK PGHFDRTLAR GPKTTTWIWN LHADAHDFDS QTSDLEDISR KIFSAHFGHL AVVFVWLSGM YFHGAKFSN YSAWLADPLN IKPSAQVVWP VVGQDILNAD VGGGFHGIQI TSGFFQLWRA SGITNEYQLY CTAIGGLVMA G LMLFAGWF HYHKAAPKLE WFQNVESMMN HHLAGLLGLG SLGWAGHQIH VSLPINKLLD AGVAPNEIPL PHEFILDPAK MA ELYPSFA QGLTPFFTLN WGVYSDFLTF KGGLNPVTGG LWLSDTAHHH LAIAVLFIIA GHMYRTNWGI GHNMKEILEG HKG PFTGEG HKGLYEILTT SWHAQLAINL ALLGSLTIIV AQHMYAMPPY PYLATDYGTQ LSIFTHHMWI GGFLIVGAGA HASI FMVRD YDPAKNVNNL LDRVLRHRDA IISHLNWVCI WLGFHSFGLY IHNDTMRALG RPQDMFSDSA IQLQPVFAQW IQGLH AAAA GATAPFASAG VSPVFGGEVV AVGGKVAMMP ITLGTADFMV HHIHAFTIHV TVLILLKGVL YSRSSRLIPD KAELGF RFP CDGPGRGGTC QVSGWDHVFL GLFWMYNSLS IVIFHFSWKM QSDVWGTVLP DGSVSHITGG NFAQSAITIN GWLRDFL WA QAANVINSYG SALSAYGIMF LAGHFVFAFS LMFLFSGRGY WQELIESIVW AHNKLKLAPA IQPRALSIVQ GRAVGVAH Y LLGGIVTTWA FFLCRILSVG

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Macromolecule #2: Photosystem I P700 chlorophyll a apoprotein A2

MacromoleculeName: Photosystem I P700 chlorophyll a apoprotein A2 / type: protein_or_peptide / ID: 2 / Number of copies: 3 / Enantiomer: LEVO / EC number: photosystem I
Source (natural)Organism: Cyanobacterium aponinum 0216 (bacteria) / Strain: 216
Molecular weightTheoretical: 81.865945 KDa
SequenceString: ATKFPKFSQD LAQDPTTRRI WYGIATAHDF ETHDGMTEEN LYQKIFASHF GHLAIIFLWT SGTVFHVAWQ GNFEQWIKDP LNVRPIAHA IWDPHFGQGA VDAFTQAGAS SPVNVAYSGV YHWFYTIGMT NNQDLYQGAV FLLILSALFL FAGWLHLQPK F RPSLSWFK ...String:
ATKFPKFSQD LAQDPTTRRI WYGIATAHDF ETHDGMTEEN LYQKIFASHF GHLAIIFLWT SGTVFHVAWQ GNFEQWIKDP LNVRPIAHA IWDPHFGQGA VDAFTQAGAS SPVNVAYSGV YHWFYTIGMT NNQDLYQGAV FLLILSALFL FAGWLHLQPK F RPSLSWFK NAESRLNHHL AGLFGVSSLA WTGHLVHVAI PESRGVHVGW DNFLSVKPHP AGLAPFFTGN WGVYAQNPDT AS HVFGTSE GAGSAILTFL GGFHPQTESL WLTDIAHHHL AIAVIFIVAG HMYRTNWGIG HSIKDILAAH NPPQGTPFGG ALG AGHRGL YDTINNSLHF QLGLALASLG VITSLVAQHM YSLPSYAFIA KDYTTQAALY THHQYIAGFL MVGAFAHGAI FFVR DYDPE ANKDNVLARM LEHKEAIISH LSWVSLFLGF HTLGLYVHND VVVAFGTPEK QILIEPVFAQ FVQAASGKAL YGFDA LLSN PDSVASTASA VWLPGWLDAI NSGTNSLFLN IGPGDFLVHH AIALGLHTTT LILVKGALDA RGSKLMPDKK DFGFSF PCD GPGRGGTCDI SAWDAFYLAM FWMLNTLGWL TFYWHWKHLG IWTGNVAQFN ENSTYLMGWF RDYLWANSAQ LINGYNP YG VNNLSVWAWM FLFGHLVWAT GFMFLISWRG YWQELIETIV WAHERTPLAN LVRWKDKPVA LSIVQARLVG LAHFTVGY I LTYAAFLIAS TAGKFG

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Macromolecule #3: Photosystem I iron-sulfur center

MacromoleculeName: Photosystem I iron-sulfur center / type: protein_or_peptide / ID: 3 / Number of copies: 3 / Enantiomer: LEVO / EC number: photosystem I
Source (natural)Organism: Cyanobacterium aponinum 0216 (bacteria) / Strain: 216
Molecular weightTheoretical: 8.692039 KDa
SequenceString:
SHSVKIYDTC IGCTQCVRAC PLDVLEMVPW DGCKAGQIAS SPRTEDCVGC KRCETACPTD FLSIRVYLGA ETTRSMGLAY

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Macromolecule #4: Photosystem I reaction center subunit II

MacromoleculeName: Photosystem I reaction center subunit II / type: protein_or_peptide / ID: 4 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Cyanobacterium aponinum 0216 (bacteria) / Strain: 216
Molecular weightTheoretical: 15.647748 KDa
SequenceString:
IQLTGTMPKF GGSTGGLLSA ADREEKYAIT WTSKTEQVFE MPTGGAAIMN EGENLLYFAR KEQCLALGTQ LRTKFKPKIE DYKIYRIYP TGETQYLHPA DGVFPEKVNE GREFHGKKDR NIGKNPEPVT LKFSGKTPYD

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Macromolecule #5: Photosystem I reaction center subunit IV

MacromoleculeName: Photosystem I reaction center subunit IV / type: protein_or_peptide / ID: 5 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Cyanobacterium aponinum 0216 (bacteria) / Strain: 216
Molecular weightTheoretical: 7.634534 KDa
SequenceString:
AIQRGSKVRI LRKESYWYKD VGTVASVDKS GILYPVIVRF DKVNYNGFSG SATGVNTNNF AEHELEEV

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Macromolecule #6: PSI-F

MacromoleculeName: PSI-F / type: protein_or_peptide / ID: 6 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Cyanobacterium aponinum 0216 (bacteria) / Strain: 216
Molecular weightTheoretical: 15.665845 KDa
SequenceString:
DLSHLTPCSE SPAYQAKAKS FRNTTSDPES GQKRAESYAE ALCGPEGYPH LVVDGRLDHA GDFIIPGLLF LYVAGWIGWV GRSYLIAIR EEKDTEMKEI IIDVPLAINK MLFGFMWPLQ AFGEFTSGKL TVKDSEIPVS PR

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Macromolecule #7: Photosystem I reaction center subunit VIII

MacromoleculeName: Photosystem I reaction center subunit VIII / type: protein_or_peptide / ID: 7 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Cyanobacterium aponinum 0216 (bacteria) / Strain: 216
Molecular weightTheoretical: 3.913726 KDa
SequenceString:
ILGDFAASFL PAILVPAVGL VMPAVVMGLL FLQIESEA

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Macromolecule #8: Photosystem I reaction center subunit IX

MacromoleculeName: Photosystem I reaction center subunit IX / type: protein_or_peptide / ID: 8 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Cyanobacterium aponinum 0216 (bacteria) / Strain: 216
Molecular weightTheoretical: 4.354158 KDa
SequenceString:
KGLTTFLSTA PVLITALLVF TAGLLIEFNR FYPDLLFHP

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Macromolecule #9: Photosystem I reaction center subunit PsaK

MacromoleculeName: Photosystem I reaction center subunit PsaK / type: protein_or_peptide / ID: 9 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Cyanobacterium aponinum 0216 (bacteria) / Strain: 216
Molecular weightTheoretical: 7.860323 KDa
SequenceString:
AVPTTLEWNL NVGLTMIVCN IVAIAFAKYT MKEPGAGPKL PGEAFFGGMG LPALLATTSF GHLLGAGVIL GLASLGAL

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Macromolecule #10: Photosystem I reaction center subunit XI

MacromoleculeName: Photosystem I reaction center subunit XI / type: protein_or_peptide / ID: 10 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Cyanobacterium aponinum 0216 (bacteria) / Strain: 216
Molecular weightTheoretical: 16.779295 KDa
SequenceString:
IDVVNHGGDP QVGNLSTPIN GSAFTKAFIN ALPAYRKGLS PNRRGLEVGM AHGYLLYGPF AVLGPLRLTE YGPTAGLLAT IGLVSILTI CLSIYGAVGV SKPTETLTTP EVPMDLATKE GWSEFAGGFL LGGCGGAFFA FFLCQTPHLQ PLIEVASNIW S

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Macromolecule #11: Photosystem I reaction center subunit XII

MacromoleculeName: Photosystem I reaction center subunit XII / type: protein_or_peptide / ID: 11 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Cyanobacterium aponinum 0216 (bacteria) / Strain: 216
Molecular weightTheoretical: 3.27892 KDa
SequenceString:
SISETQVFVA LVIALIPGIL AFRLATELYK

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Macromolecule #12: CHLOROPHYLL A ISOMER

MacromoleculeName: CHLOROPHYLL A ISOMER / type: ligand / ID: 12 / Number of copies: 3 / Formula: CL0
Molecular weightTheoretical: 893.489 Da
Chemical component information

ChemComp-CL0:
CHLOROPHYLL A ISOMER / Chlorophyll a

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Macromolecule #13: CHLOROPHYLL A

MacromoleculeName: CHLOROPHYLL A / type: ligand / ID: 13 / Number of copies: 285 / Formula: CLA
Molecular weightTheoretical: 893.489 Da
Chemical component information

ChemComp-CLA:
CHLOROPHYLL A / Chlorophyll a

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Macromolecule #14: PHYLLOQUINONE

MacromoleculeName: PHYLLOQUINONE / type: ligand / ID: 14 / Number of copies: 6 / Formula: PQN
Molecular weightTheoretical: 450.696 Da
Chemical component information

ChemComp-PQN:
PHYLLOQUINONE / Phytomenadione

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Macromolecule #15: IRON/SULFUR CLUSTER

MacromoleculeName: IRON/SULFUR CLUSTER / type: ligand / ID: 15 / Number of copies: 9 / Formula: SF4
Molecular weightTheoretical: 351.64 Da
Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER / Iron–sulfur cluster

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Macromolecule #16: BETA-CAROTENE

MacromoleculeName: BETA-CAROTENE / type: ligand / ID: 16 / Number of copies: 69 / Formula: BCR
Molecular weightTheoretical: 536.873 Da
Chemical component information

ChemComp-BCR:
BETA-CAROTENE / Β-Carotene

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Macromolecule #17: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE

MacromoleculeName: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / type: ligand / ID: 17 / Number of copies: 6 / Formula: LHG
Molecular weightTheoretical: 722.97 Da
Chemical component information

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM / Phosphatidylglycerol

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Macromolecule #18: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

MacromoleculeName: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE / type: ligand / ID: 18 / Number of copies: 3 / Formula: LMG
Molecular weightTheoretical: 787.158 Da
Chemical component information

ChemComp-LMG:
1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

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Macromolecule #19: 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL

MacromoleculeName: 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL
type: ligand / ID: 19 / Number of copies: 3 / Formula: SQD
Molecular weightTheoretical: 795.116 Da
Chemical component information

ChemComp-SQD:
1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 8
GridDetails: unspecified
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Average electron dose: 1.53 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionSoftware - Name: CTFFIND (ver. 4.1)
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.0.7)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.0.7)
Final reconstructionApplied symmetry - Point group: C3 (3 fold cyclic) / Resolution.type: BY AUTHOR / Resolution: 2.7 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION / Number images used: 75290
FSC plot (resolution estimation)

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