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- EMDB-1973: Electron Cryotomography of Measles Virus Reveals how Matrix Prote... -
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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-1973 | |||||||||
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Title | Electron Cryotomography of Measles Virus Reveals how Matrix Protein Coats the Ribonucleocapsid Within Intact Virions. | |||||||||
![]() | Measles virus nucleocapsid filament | |||||||||
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![]() | measles / matrix / nucleocapsid / ribonucleocapsid / RNP / MCNC | |||||||||
Biological species | ![]() | |||||||||
Method | subtomogram averaging / cryo EM | |||||||||
![]() | Liljeroos L / Huiskonen JT / Ora A / Susi P / Butcher SJ | |||||||||
![]() | ![]() Title: Electron cryotomography of measles virus reveals how matrix protein coats the ribonucleocapsid within intact virions. Authors: Lassi Liljeroos / Juha T Huiskonen / Ari Ora / Petri Susi / Sarah J Butcher / ![]() Abstract: Measles virus is a highly infectious, enveloped, pleomorphic virus. We combined electron cryotomography with subvolume averaging and immunosorbent electron microscopy to characterize the 3D ...Measles virus is a highly infectious, enveloped, pleomorphic virus. We combined electron cryotomography with subvolume averaging and immunosorbent electron microscopy to characterize the 3D ultrastructure of the virion. We show that the matrix protein forms helices coating the helical ribonucleocapsid rather than coating the inner leaflet of the membrane, as previously thought. The ribonucleocapsid is folded into tight bundles through matrix-matrix interactions. The implications for virus assembly are that the matrix already tightly interacts with the ribonucleocapsid in the cytoplasm, providing a structural basis for the previously observed regulation of RNA transcription by the matrix protein. Next, the matrix-covered ribonucleocapsids are transported to the plasma membrane, where the matrix interacts with the envelope glycoproteins during budding. These results are relevant to the nucleocapsid organization and budding of other paramyxoviruses, where isolated matrix has been observed to form helices. | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 1.4 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 8.1 KB 8.1 KB | Display Display | ![]() |
Images | ![]() | 62.9 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | Measles virus nucleocapsid filament | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 7.68 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire : Measles virus matrix-covered ribonucleocapsid, inner helix
Entire | Name: Measles virus matrix-covered ribonucleocapsid, inner helix |
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Components |
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-Supramolecule #1000: Measles virus matrix-covered ribonucleocapsid, inner helix
Supramolecule | Name: Measles virus matrix-covered ribonucleocapsid, inner helix type: sample / ID: 1000 / Number unique components: 1 |
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-Macromolecule #1: ribonucleocapsid
Macromolecule | Name: ribonucleocapsid / type: protein_or_peptide / ID: 1 / Name.synonym: ribonucleocapsid / Recombinant expression: No / Database: NCBI |
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Source (natural) | Organism: ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | subtomogram averaging |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.4 / Details: 20 mM Tris-HCl, 180 mM NaCl, pH 7.4 |
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Grid | Details: C-flat 2/2-2C, holey carbon copper grid |
Vitrification | Cryogen name: ETHANE / Instrument: HOMEMADE PLUNGER / Details: Vitrification instrument: Homemade plunger / Method: Blot for 4 seconds before plunging |
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Electron microscopy
Microscope | FEI TECNAI F20 |
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Image recording | Category: CCD / Film or detector model: GATAN ULTRASCAN 4000 (4k x 4k) |
Electron beam | Acceleration voltage: 200 kV / Electron source: ![]() |
Electron optics | Calibrated magnification: 39400 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.0 mm / Nominal defocus max: 6.0 µm / Nominal defocus min: 6.0 µm / Nominal magnification: 39400 |
Sample stage | Specimen holder: Eucentric / Specimen holder model: GATAN LIQUID NITROGEN / Tilt series - Axis1 - Min angle: -60 ° / Tilt series - Axis1 - Max angle: 60 ° |
Experimental equipment | ![]() Model: Tecnai F20 / Image courtesy: FEI Company |
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Image processing
Details | Average number of projections used in the 3D reconstructions: 772. |
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Final reconstruction | Software - Name: IMOD, Bsoft, Jsubtomo |