- EMDB-19723: RNA polymerase II initially transcribing complex with an ordered ... -
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基本情報
登録情報
データベース: EMDB / ID: EMD-19723
タイトル
RNA polymerase II initially transcribing complex with an ordered RNA of 12 nt
マップデータ
試料
複合体: mammalian RNA polymerase II general transcription factor IIA general transcription factor IIB general transcription factor IIE general transcription factor IIF general transcription factor IIH TATA-box binding protein DNA
複合体: RNA polymerase II
キーワード
RNA polymerase II / promoter escape / de novo initiation / TRANSCRIPTION
ジャーナル: Mol Cell / 年: 2024 タイトル: Three-step mechanism of promoter escape by RNA polymerase II. 著者: Yumeng Zhan / Frauke Grabbe / Elisa Oberbeckmann / Christian Dienemann / Patrick Cramer / 要旨: The transition from transcription initiation to elongation is highly regulated in human cells but remains incompletely understood at the structural level. In particular, it is unclear how ...The transition from transcription initiation to elongation is highly regulated in human cells but remains incompletely understood at the structural level. In particular, it is unclear how interactions between RNA polymerase II (RNA Pol II) and initiation factors are broken to enable promoter escape. Here, we reconstitute RNA Pol II promoter escape in vitro and determine high-resolution structures of initially transcribing complexes containing 8-, 10-, and 12-nt ordered RNAs and two elongation complexes containing 14-nt RNAs. We suggest that promoter escape occurs in three major steps. First, the growing RNA displaces the B-reader element of the initiation factor TFIIB without evicting TFIIB. Second, the rewinding of the transcription bubble coincides with the eviction of TFIIA, TFIIB, and TBP. Third, the binding of DSIF and NELF facilitates TFIIE and TFIIH dissociation, establishing the paused elongation complex. This three-step model for promoter escape fills a gap in our understanding of the initiation-elongation transition of RNA Pol II transcription.
全体 : mammalian RNA polymerase II general transcription factor IIA gene...
全体
名称: mammalian RNA polymerase II general transcription factor IIA general transcription factor IIB general transcription factor IIE general transcription factor IIF general transcription factor ...名称: mammalian RNA polymerase II general transcription factor IIA general transcription factor IIB general transcription factor IIE general transcription factor IIF general transcription factor IIH TATA-box binding protein DNA
要素
複合体: mammalian RNA polymerase II general transcription factor IIA general transcription factor IIB general transcription factor IIE general transcription factor IIF general transcription factor IIH TATA-box binding protein DNA
複合体: RNA polymerase II
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超分子 #1: mammalian RNA polymerase II general transcription factor IIA gene...
超分子
名称: mammalian RNA polymerase II general transcription factor IIA general transcription factor IIB general transcription factor IIE general transcription factor IIF general transcription factor ...名称: mammalian RNA polymerase II general transcription factor IIA general transcription factor IIB general transcription factor IIE general transcription factor IIF general transcription factor IIH TATA-box binding protein DNA タイプ: complex / ID: 1 / 親要素: 0