Biotechnology and Biological Sciences Research Council (BBSRC)
BB/P000940/1
United Kingdom
Wellcome Trust
202904/Z/16/Z
United Kingdom
Wellcome Trust
206181/Z/17/Z
United Kingdom
Biotechnology and Biological Sciences Research Council (BBSRC)
BB/L01386X/1
United Kingdom
Wellcome Trust
210701/Z/18/Z
United Kingdom
Wellcome Trust
106115/Z/14/Z
United Kingdom
Biotechnology and Biological Sciences Research Council (BBSRC)
BB/R000484/1
United Kingdom
Citation
Journal: Nat Commun / Year: 2022 Title: Structural insights in cell-type specific evolution of intra-host diversity by SARS-CoV-2. Authors: Kapil Gupta / Christine Toelzer / Maia Kavanagh Williamson / Deborah K Shoemark / A Sofia F Oliveira / David A Matthews / Abdulaziz Almuqrin / Oskar Staufer / Sathish K N Yadav / Ufuk Borucu ...Authors: Kapil Gupta / Christine Toelzer / Maia Kavanagh Williamson / Deborah K Shoemark / A Sofia F Oliveira / David A Matthews / Abdulaziz Almuqrin / Oskar Staufer / Sathish K N Yadav / Ufuk Borucu / Frederic Garzoni / Daniel Fitzgerald / Joachim Spatz / Adrian J Mulholland / Andrew D Davidson / Christiane Schaffitzel / Imre Berger / Abstract: As the global burden of SARS-CoV-2 infections escalates, so does the evolution of viral variants with increased transmissibility and pathology. In addition to this entrenched diversity, RNA viruses ...As the global burden of SARS-CoV-2 infections escalates, so does the evolution of viral variants with increased transmissibility and pathology. In addition to this entrenched diversity, RNA viruses can also display genetic diversity within single infected hosts with co-existing viral variants evolving differently in distinct cell types. The BriSΔ variant, originally identified as a viral subpopulation from SARS-CoV-2 isolate hCoV-19/England/02/2020, comprises in the spike an eight amino-acid deletion encompassing a furin recognition motif and S1/S2 cleavage site. We elucidate the structure, function and molecular dynamics of this spike providing mechanistic insight into how the deletion correlates to viral cell tropism, ACE2 receptor binding and infectivity of this SARS-CoV-2 variant. Our results reveal long-range allosteric communication between functional domains that differ in the wild-type and the deletion variant and support a view of SARS-CoV-2 probing multiple evolutionary trajectories in distinct cell types within the same infected host.
History
Deposition
Apr 29, 2021
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Header (metadata) release
Jan 26, 2022
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Map release
Jan 26, 2022
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Update
Jan 26, 2022
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Current status
Jan 26, 2022
Processing site: PDBe / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Number grids imaged: 1 / Number real images: 8639 / Average exposure time: 11.0 sec. / Average electron dose: 63.8 e/Å2
Electron beam
Acceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
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