登録情報 データベース : EMDB / ID : EMD-12289 構造の表示 ダウンロードとリンクタイトル Structure of the in situ actomyosin complex from the A-band of mouse psoas muscle sarcomere in the rigor state obtained by sub-tomogram averaging マップデータAveraged map of in situ actomyosin complex 詳細 試料複合体 : In situ actomyosin complex in the rigor state from mouse psoas muscle複合体 : actinタンパク質・ペプチド : Actin, alpha skeletal muscle複合体 : myosin double headタンパク質・ペプチド : Myosin-4タンパク質・ペプチド : Myosin light chain 1/3, skeletal muscle isoformタンパク質・ペプチド : Myosin regulatory light chain 2, skeletal muscle isoform複合体 : tropomyosinタンパク質・ペプチド : Tropomyosin alpha-1 chain 詳細 キーワード Muscle proteins / force generation / sarcomere / cytoskeleton / MOTOR PROTEIN機能・相同性 機能・相同性情報分子機能 ドメイン・相同性 構成要素
positive regulation of heart rate by epinephrine / muscle thin filament tropomyosin / skeletal muscle fiber adaptation / Striated Muscle Contraction / Smooth Muscle Contraction / contractile muscle fiber / muscle filament sliding / : / myosin filament / actin filament capping ... positive regulation of heart rate by epinephrine / muscle thin filament tropomyosin / skeletal muscle fiber adaptation / Striated Muscle Contraction / Smooth Muscle Contraction / contractile muscle fiber / muscle filament sliding / : / myosin filament / actin filament capping / : / ruffle organization / myosin complex / response to steroid hormone / ventricular cardiac muscle tissue morphogenesis / structural constituent of muscle / myosin II complex / microfilament motor activity / myofibril / mesenchyme migration / skeletal muscle thin filament assembly / striated muscle thin filament / positive regulation of cell adhesion / skeletal muscle fiber development / cardiac muscle contraction / response to mechanical stimulus / stress fiber / skeletal muscle tissue development / positive regulation of stress fiber assembly / negative regulation of cell migration / response to activity / filopodium / muscle contraction / actin filament / actin filament organization / 加水分解酵素; 酸無水物に作用; 酸無水物に作用・細胞または細胞小器官の運動に関与 / wound healing / structural constituent of cytoskeleton / double-stranded RNA binding / disordered domain specific binding / actin filament binding / actin cytoskeleton / lamellipodium / actin binding / cell body / in utero embryonic development / hydrolase activity / calmodulin binding / immune response / protein heterodimerization activity / calcium ion binding / positive regulation of gene expression / protein homodimerization activity / protein-containing complex / ATP binding / identical protein binding / cytoplasm 類似検索 - 分子機能 Tropomyosins signature. / Tropomyosin / Tropomyosin / EF-hand domain / DNA repair protein XRCC4-like, C-terminal / Myosin tail / Myosin tail / Myosin N-terminal SH3-like domain / Myosin S1 fragment, N-terminal / Myosin, N-terminal, SH3-like ... Tropomyosins signature. / Tropomyosin / Tropomyosin / EF-hand domain / DNA repair protein XRCC4-like, C-terminal / Myosin tail / Myosin tail / Myosin N-terminal SH3-like domain / Myosin S1 fragment, N-terminal / Myosin, N-terminal, SH3-like / Myosin N-terminal SH3-like domain profile. / Myosin head, motor domain / Myosin head (motor domain) / Myosin motor domain profile. / Myosin. Large ATPases. / IQ motif profile. / IQ motif, EF-hand binding site / Actins signature 1. / Actin, conserved site / Actins signature 2. / Kinesin motor domain superfamily / Actin/actin-like conserved site / Actins and actin-related proteins signature. / Actin / Actin family / Actin / EF-hand, calcium binding motif / ATPase, nucleotide binding domain / EF-Hand 1, calcium-binding site / EF-hand calcium-binding domain. / EF-hand calcium-binding domain profile. / EF-hand domain / EF-hand domain pair / P-loop containing nucleoside triphosphate hydrolase 類似検索 - ドメイン・相同性 Myosin light chain 1/3, skeletal muscle isoform / Tropomyosin alpha-1 chain / Actin, alpha skeletal muscle / Myosin regulatory light chain 11 / Myosin-4 類似検索 - 構成要素生物種 Mus musculus (ハツカネズミ)手法 サブトモグラム平均法 / クライオ電子顕微鏡法 / 解像度 : 10.2 Å 詳細 データ登録者Wang Z / Grange M 資金援助 ドイツ, 英国, European Union, 5件 詳細 詳細を隠すOrganization Grant number 国 Max Planck Society ドイツ Wellcome Trust 201543/Z/16/Z 英国 European Research Council (ERC) 856118 European Union Medical Research Council (MRC, United Kingdom) MR/R003106/1 英国 European Molecular Biology Organization (EMBO) ALTF 693-2018 European Union
引用ジャーナル : Cell / 年 : 2021タイトル : The molecular basis for sarcomere organization in vertebrate skeletal muscle.著者 : Zhexin Wang / Michael Grange / Thorsten Wagner / Ay Lin Kho / Mathias Gautel / Stefan Raunser / 要旨 : Sarcomeres are force-generating and load-bearing devices of muscles. A precise molecular picture of how sarcomeres are built underpins understanding their role in health and disease. Here, we ... Sarcomeres are force-generating and load-bearing devices of muscles. A precise molecular picture of how sarcomeres are built underpins understanding their role in health and disease. Here, we determine the molecular architecture of native vertebrate skeletal sarcomeres by electron cryo-tomography. Our reconstruction reveals molecular details of the three-dimensional organization and interaction of actin and myosin in the A-band, I-band, and Z-disc and demonstrates that α-actinin cross-links antiparallel actin filaments by forming doublets with 6-nm spacing. Structures of myosin, tropomyosin, and actin at ~10 Å further reveal two conformations of the "double-head" myosin, where the flexible orientation of the lever arm and light chains enable myosin not only to interact with the same actin filament, but also to split between two actin filaments. Our results provide unexpected insights into the fundamental organization of vertebrate skeletal muscle and serve as a strong foundation for future investigations of muscle diseases. 履歴 登録 2021年2月4日 - ヘッダ(付随情報) 公開 2021年4月7日 - マップ公開 2021年4月7日 - 更新 2024年5月1日 - 現状 2024年5月1日 処理サイト : PDBe / 状態 : 公開
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