: / Pseudouridine synthase, TruD / Pseudouridine synthase, TruD, catalytic domain / : / GTPase Der, C-terminal KH-domain-like / KH-domain-like of EngA bacterial GTPase enzymes, C-terminal / Pseudouridine synthase, RsuA/RluA-like / : / RNA pseudouridylate synthase / tRNA/rRNA methyltransferase, SpoU type ...: / Pseudouridine synthase, TruD / Pseudouridine synthase, TruD, catalytic domain / : / GTPase Der, C-terminal KH-domain-like / KH-domain-like of EngA bacterial GTPase enzymes, C-terminal / Pseudouridine synthase, RsuA/RluA-like / : / RNA pseudouridylate synthase / tRNA/rRNA methyltransferase, SpoU type / SpoU rRNA Methylase family / Fungal lipase-like domain / Lipase (class 3) / Protein Iojap/ribosomal silencing factor RsfS / Ribosomal silencing factor during starvation / Pseudouridine synthase, catalytic domain superfamily / LIM domain / Zinc-binding domain present in Lin-11, Isl-1, Mec-3. / Zinc finger, LIM-type / GTP-binding protein, orthogonal bundle domain superfamily / LIM domain profile. / Pentatricopeptide (PPR) repeat profile. / DnaJ domain / Pentatricopeptide repeat / Rhodanese-like domain superfamily / 39S ribosomal protein L43/54S ribosomal protein L51 / Ribosomal protein L47, mitochondrial / MRP-L47 superfamily, mitochondrial / Mitochondrial 39-S ribosomal protein L47 (MRP-L47) / DnaJ molecular chaperone homology domain / dnaJ domain profile. / Chaperone J-domain superfamily / tRNA (guanine-N1-)-methyltransferase, N-terminal / DnaJ domain / Alpha/beta knot methyltransferases / 50S ribosome-binding GTPase / RNA helicase, DEAD-box type, Q motif / Cyclophilin-type peptidyl-prolyl cis-trans isomerase / DEAD-box RNA helicase Q motif profile. / GTP binding domain / Cyclophilin-type peptidyl-prolyl cis-trans isomerase domain profile. / Cyclophilin-type peptidyl-prolyl cis-trans isomerase domain / Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD / Cyclophilin-like domain superfamily / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / Ribosomal protein/NADH dehydrogenase domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / Acyl carrier protein (ACP) / Nucleotidyltransferase superfamily / DEAD/DEAH box helicase domain / DEAD/DEAH box helicase / Phosphopantetheine attachment site / Phosphopantetheine attachment site. / Ribosomal protein L11, N-terminal / Ribosomal protein L11, N-terminal domain / Phosphopantetheine attachment site / Ribosomal protein L11/L12 / Ribosomal protein L11, C-terminal / Ribosomal protein L11, C-terminal domain superfamily / Ribosomal protein L11/L12, N-terminal domain superfamily / Ribosomal protein L11/L12 / Ribosomal protein L11, RNA binding domain / ACP-like superfamily / Carrier protein (CP) domain profile. / Phosphopantetheine binding ACP domain / Ribosomal protein L9 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / 50S ribosomal protein L30e-like / Helicase conserved C-terminal domain / Ribosomal protein L28/L24 / : / Ribosomal protein L20 / Ribosomal protein L20, C-terminal / Ribosomal protein L21 / Ribosomal protein L17 / Ribosomal protein L17 superfamily / Ribosomal protein L17 / Ribosomal protein L21-like / L21-like superfamily / Ribosomal prokaryotic L21 protein / Ribosomal protein L24 / Ribosomal protein L3, bacterial/organelle-type / Ribosomal protein L15, bacterial-type / 50S ribosomal protein uL4 / Small GTP-binding protein domain / Prokaryotic membrane lipoprotein lipid attachment site profile. / K homology domain-like, alpha/beta / Tetratricopeptide-like helical domain superfamily / helicase superfamily c-terminal domain / Ribosomal protein L13 / Ribosomal protein L13 / Ribosomal protein L13 superfamily / Ribosomal protein L25/L23 / Ribosomal protein L30, ferredoxin-like fold domain superfamily / Alpha/Beta hydrolase fold / Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A / Ribosomal protein L14P / Ribosomal protein L14 superfamily / Ribosomal protein L14p/L23e 類似検索 - ドメイン・相同性
Uncharacterized protein / Uncharacterized protein / Peptidyl-prolyl cis-trans isomerase / Uncharacterized protein / Uncharacterized protein / Large ribosomal subunit protein uL4m / IQ calmodulin-binding motif containing protein / Large ribosomal subunit protein uL23m / Uncharacterized protein / Uncharacterized protein ...Uncharacterized protein / Uncharacterized protein / Peptidyl-prolyl cis-trans isomerase / Uncharacterized protein / Uncharacterized protein / Large ribosomal subunit protein uL4m / IQ calmodulin-binding motif containing protein / Large ribosomal subunit protein uL23m / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Mitochondrial RNA binding complex 1 subunit / Uncharacterized protein / GTP-binding protein / Uncharacterized protein / Lipase (Class 3) / SpoU rRNA Methylase family / Uncharacterized protein / Uncharacterized protein / Rhodanese-like domain containing protein / Uncharacterized protein / Ribosomal protein L30p/L7e / Uncharacterized protein / Ribosomal protein L22p/L17e / Uncharacterized protein / LIM domain containing protein / 39S ribosomal protein L28 / Uncharacterized protein / Large ribosomal subunit protein uL15/eL18 domain-containing protein / Oligomerization domain containing protein / Uncharacterized protein / Complex1-LYR-like / Uncharacterized protein / Ribosomal protein L9 domain-containing protein / Large ribosomal subunit protein bL21m / Tetratricopeptide repeat / 50S ribosomal protein L13 / 50S ribosomal protein L17 / Uncharacterized protein / Large ribosomal subunit protein mL43 / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / 50S ribosomal protein L14 / Ribosomal protein L11 / KOW domain-containing protein / Uncharacterized protein / Chaperone protein DNAj, putative / GTP-binding protein, putative / Uncharacterized protein / T. brucei spp.-specific protein / Uncharacterized protein / Mitochondrial RNA binding protein / TRUD domain-containing protein / DEAD-box helicase, putative / Uncharacterized protein / Large ribosomal subunit protein uL29m / Uncharacterized protein / Pseudouridylate synthase RPUSD4, mitochondrial / Uncharacterized protein / Uncharacterized protein / Peptidyl-prolyl cis-trans isomerase / Uncharacterized protein / Acyl carrier protein / Uncharacterized protein / Large ribosomal subunit protein uL3m 類似検索 - 構成要素
ジャーナル: EMBO J / 年: 2021 タイトル: Interconnected assembly factors regulate the biogenesis of mitoribosomal large subunit. 著者: Victor Tobiasson / Ondřej Gahura / Shintaro Aibara / Rozbeh Baradaran / Alena Zíková / Alexey Amunts / 要旨: Mitoribosomes consist of ribosomal RNA and protein components, coordinated assembly of which is critical for function. We used mitoribosomes from Trypanosoma brucei with reduced RNA and increased ...Mitoribosomes consist of ribosomal RNA and protein components, coordinated assembly of which is critical for function. We used mitoribosomes from Trypanosoma brucei with reduced RNA and increased protein mass to provide insights into the biogenesis of the mitoribosomal large subunit. Structural characterization of a stable assembly intermediate revealed 22 assembly factors, some of which have orthologues/counterparts/homologues in mammalian genomes. These assembly factors form a protein network that spans a distance of 180 Å, shielding the ribosomal RNA surface. The central protuberance and L7/L12 stalk are not assembled entirely and require removal of assembly factors and remodeling of the mitoribosomal proteins to become functional. The conserved proteins GTPBP7 and mt-EngA are bound together at the subunit interface in proximity to the peptidyl transferase center. A mitochondrial acyl-carrier protein plays a role in docking the L1 stalk, which needs to be repositioned during maturation. Additional enzymatically deactivated factors scaffold the assembly while the exit tunnel is blocked. Together, this extensive network of accessory factors stabilizes the immature sites and connects the functionally important regions of the mitoribosomal large subunit.