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- EMDB-11569: Human C Complex Spliceosome - High-resolution CORE -

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Basic information

Entry
Database: EMDB / ID: EMD-11569
TitleHuman C Complex Spliceosome - High-resolution CORE
Map data
SampleHuman C complex Spliceosome - High resolution core
  • (nucleic-acidNucleic acid) x 4
  • Corepressor interacting with RBPJ 1
  • Pre-mRNA-processing-splicing factor 8
  • 116 kDa U5 small nuclear ribonucleoprotein component
  • SNW domain-containing protein 1
  • Pleiotropic regulator 1
  • Pre-mRNA-processing factor 17
  • U5 small nuclear ribonucleoprotein 40 kDa protein
  • Cell division cycle 5-like protein
  • Crooked neck-like protein 1
  • (Pre-mRNA-splicing factor ...) x 5
  • Protein BUD31 homolog
  • Spliceosome-associated protein CWC15 homolog
  • Serine/arginine repetitive matrix protein 2
  • Peptidyl-prolyl cis-trans isomerase-like 1
  • WD repeat-containing protein 70
  • Splicing factor YJU2
  • Protein FRG1
  • (ligand) x 4
Function / homology
Function and homology information


striated muscle dense body / regulation of histone H2B conserved C-terminal lysine ubiquitination / regulation of DNA double-strand break processing / positive regulation of vitamin D receptor signaling pathway / post-spliceosomal complex / cellular response to interleukin-2 / cellular response to wortmannin / cellular response to prolactin / leucine zipper domain binding / signal transduction involved in DNA damage checkpoint ...striated muscle dense body / regulation of histone H2B conserved C-terminal lysine ubiquitination / regulation of DNA double-strand break processing / positive regulation of vitamin D receptor signaling pathway / post-spliceosomal complex / cellular response to interleukin-2 / cellular response to wortmannin / cellular response to prolactin / leucine zipper domain binding / signal transduction involved in DNA damage checkpoint / transcription corepressor binding / regulation of vitamin D receptor signaling pathway / post-mRNA release spliceosomal complex / RNA splicing, via transesterification reactions / positive regulation of androgen receptor activity / U2-type spliceosomal complex / positive regulation of neurogenesis / protein phosphatase 1 binding / U2-type catalytic step 1 spliceosome / WD40-repeat domain binding / U2-type precatalytic spliceosome / positive regulation of mRNA splicing, via spliceosome / regulation of retinoic acid receptor signaling pathway / C2H2 zinc finger domain binding / retinoic acid receptor binding / pre-mRNA binding / positive regulation by host of viral transcription / regulation of mRNA splicing, via spliceosome / Prp19 complex / vitamin D receptor binding / mRNA cis splicing, via spliceosome / U2-type catalytic step 2 spliceosome / Notch binding / K63-linked polyubiquitin modification-dependent protein binding / Cul4-RING E3 ubiquitin ligase complex / mRNA 3'-end processing / generation of catalytic spliceosome for second transesterification step / spliceosomal complex assembly / cellular response to fibroblast growth factor stimulus / muscle organ development / small nucleolar ribonucleoprotein complex / positive regulation of transcription of Notch receptor target / mRNA 3'-splice site recognition / RNA export from nucleus / cyclosporin A binding / spliceosomal tri-snRNP complex assembly / retinoic acid receptor signaling pathway / positive regulation of transforming growth factor beta receptor signaling pathway / U5 snRNP / U5 snRNA binding / U2 snRNA binding / positive regulation of histone H3-K4 methylation / RNA processing / positive regulation of Notch signaling pathway / U6 snRNA binding / protein localization to nucleus / pre-mRNA intronic binding / negative regulation of DNA damage response, signal transduction by p53 class mediator / U4/U6 x U5 tri-snRNP complex / U1 snRNA binding / intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / SMAD binding / positive regulation of G1/S transition of mitotic cell cycle / catalytic step 2 spliceosome / response to cocaine / Cajal body / cellular response to nerve growth factor stimulus / mRNA export from nucleus / protein peptidyl-prolyl isomerization / spliceosomal complex / positive regulation of RNA splicing / RNA splicing / androgen receptor binding / cellular response to retinoic acid / microtubule organizing center / nuclear hormone receptor binding / peptidylprolyl isomerase / peptidyl-prolyl cis-trans isomerase activity / fibrillar center / positive regulation of protein export from nucleus / protein-DNA complex / Notch signaling pathway / nuclear receptor transcription coactivator activity / rRNA processing / mRNA splicing, via spliceosome / calcium-dependent protein binding / site of double-strand break / nuclear matrix / transcription corepressor activity / positive regulation of protein import into nucleus / transcription-coupled nucleotide-excision repair / cellular response to drug / mRNA processing / Z disc / histone deacetylase binding / actin filament binding / cellular response to tumor necrosis factor / RNA polymerase II transcription regulatory region sequence-specific DNA binding / ribonucleoprotein complex / mitotic cell cycle
MIF4G-like domain superfamily / MIF4G-like, type 3 / Cyclophilin-type peptidyl-prolyl cis-trans isomerase, conserved site / Pre-mRNA splicing factor component Cdc5p/Cef1 / Cyclophilin-type peptidyl-prolyl cis-trans isomerase domain / PRP8 domain IV core / Pre-mRNA splicing factor / DNA/RNA helicase, ATP-dependent, DEAH-box type, conserved site / HAT (Half-A-TPR) repeat / SKI-interacting protein SKIP, SNW domain ...MIF4G-like domain superfamily / MIF4G-like, type 3 / Cyclophilin-type peptidyl-prolyl cis-trans isomerase, conserved site / Pre-mRNA splicing factor component Cdc5p/Cef1 / Cyclophilin-type peptidyl-prolyl cis-trans isomerase domain / PRP8 domain IV core / Pre-mRNA splicing factor / DNA/RNA helicase, ATP-dependent, DEAH-box type, conserved site / HAT (Half-A-TPR) repeat / SKI-interacting protein SKIP, SNW domain / Ribosomal protein S5 domain 2-type fold / Translation elongation factor EFTu-like, domain 2 / Cyclophilin-type peptidyl-prolyl cis-trans isomerase / Small GTP-binding protein domain / Translation elongation factor EFG/EF2, domain IV / Pre-mRNA-splicing factor Cwf15/Cwc15 / Helicase-associated domain / Spt5 C-terminal domain / G10 protein / G-protein beta WD-40 repeat / P-loop containing nucleoside triphosphate hydrolase / SKI-interacting protein, SKIP / Myb domain / WD40-repeat-containing domain / BUD31/G10-related, conserved site / CBF1-interacting co-repressor CIR, N-terminal domain / Pre-mRNA-processing-splicing factor 8, U6-snRNA-binding / Armadillo-type fold / Pre-mRNA-processing-splicing factor 8, U5-snRNA-binding / RNA recognition motif domain / WD40 repeat / RNA recognition motif, spliceosomal PrP8 / WD40 repeat, conserved site / JAB1/MPN/MOV34 metalloenzyme domain / Zinc finger, CCCH-type / Elongation factor EFG, domain V-like / Translational (tr)-type GTP-binding domain / SANT/Myb domain / Helicase, C-terminal / Saf4/Yju2 protein / Initiation factor eIF-4 gamma, MA3 / Actin-crosslinking / Pre-mRNA-processing-splicing factor 8 / Tetratricopeptide-like helical domain superfamily / Corepressor interacting with RBPJ 1 / Elongation Factor G, domain II / Ribonuclease H-like superfamily / Pre-mRNA-processing-splicing factor 8, U5-snRNA-binding domain superfamily / Prp8 RNase domain IV, palm region / PRO8NT domain / Nucleotide-binding alpha-beta plait domain superfamily / MPN domain / PROCT domain / Tetratricopeptide repeat-containing domain / mRNA splicing factor Cwf21 domain / Helicase superfamily 1/2, ATP-binding domain / Ribosomal protein S5 domain 2-type fold, subgroup / WD40/YVTN repeat-like-containing domain superfamily / Prp8 RNase domain IV, fingers region / Pre-mRNA-splicing factor Cwc2/Slt11 / PROCN domain / Pre-mRNA-splicing factor Isy1 superfamily / Protein FRG1 / Pre-mRNA-processing factor 17 / Homeobox-like domain superfamily / 116kDa U5 small nuclear ribonucleoprotein component, N-terminal / Helix hairpin bin domain superfamily / Pre-mRNA-splicing factor Isy1 / Domain of unknown function DUF1605 / Translation protein, beta-barrel domain superfamily / Cyclophilin-like domain superfamily / EF-G domain III/V-like / 116kDa U5 small nuclear ribonucleoprotein component, C-terminal / RNA-binding domain superfamily / WD40-repeat-containing domain superfamily / DEAD/DEAH box helicase domain / Zinc finger, CCCH-type superfamily
Serine/arginine repetitive matrix protein 2 / Pre-mRNA-splicing factor ISY1 homolog / Spliceosome-associated protein CWC15 homolog / Pre-mRNA-splicing factor CWC25 homolog / WD repeat-containing protein 70 / Pre-mRNA-splicing factor RBM22 / Pre-mRNA-splicing factor CWC22 homolog / Crooked neck-like protein 1 / Splicing factor YJU2 / U5 small nuclear ribonucleoprotein 40 kDa protein ...Serine/arginine repetitive matrix protein 2 / Pre-mRNA-splicing factor ISY1 homolog / Spliceosome-associated protein CWC15 homolog / Pre-mRNA-splicing factor CWC25 homolog / WD repeat-containing protein 70 / Pre-mRNA-splicing factor RBM22 / Pre-mRNA-splicing factor CWC22 homolog / Crooked neck-like protein 1 / Splicing factor YJU2 / U5 small nuclear ribonucleoprotein 40 kDa protein / Cell division cycle 5-like protein / Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 / Corepressor interacting with RBPJ 1 / Pre-mRNA-processing-splicing factor 8 / 116 kDa U5 small nuclear ribonucleoprotein component / Protein FRG1 / SNW domain-containing protein 1 / Protein BUD31 homolog / Pre-mRNA-processing factor 17 / Pleiotropic regulator 1 / Peptidyl-prolyl cis-trans isomerase-like 1
Biological speciesHomo sapiens (human) / Human (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.4 Å
AuthorsBertram K / Kastner B
Funding support Germany, 2 items
OrganizationGrant numberCountry
Max Planck Society Germany
German Research Foundation (DFG)SFB 860 Germany
CitationJournal: Mol Cell / Year: 2020
Title: Structural Insights into the Roles of Metazoan-Specific Splicing Factors in the Human Step 1 Spliceosome.
Authors: Karl Bertram / Leyla El Ayoubi / Olexandr Dybkov / Dmitry E Agafonov / Cindy L Will / Klaus Hartmuth / Henning Urlaub / Berthold Kastner / Holger Stark / Reinhard Lührmann /
Abstract: Human spliceosomes contain numerous proteins absent in yeast, whose functions remain largely unknown. Here we report a 3D cryo-EM structure of the human spliceosomal C complex at 3.4 Å core ...Human spliceosomes contain numerous proteins absent in yeast, whose functions remain largely unknown. Here we report a 3D cryo-EM structure of the human spliceosomal C complex at 3.4 Å core resolution and 4.5-5.7 Å at its periphery, and aided by protein crosslinking we determine its molecular architecture. Our structure provides additional insights into the spliceosome's architecture between the catalytic steps of splicing, and how proteins aid formation of the spliceosome's catalytically active RNP (ribonucleoprotein) conformation. It reveals the spatial organization of the metazoan-specific proteins PPWD1, WDR70, FRG1, and CIR1 in human C complexes, indicating they stabilize functionally important protein domains and RNA structures rearranged/repositioned during the B to C transition. Structural comparisons with human B, C, and P complexes reveal an intricate cascade of RNP rearrangements during splicing catalysis, with intermediate RNP conformations not found in yeast, and additionally elucidate the structural basis for the sequential recruitment of metazoan-specific spliceosomal proteins.
Validation ReportPDB-ID: 6zym

SummaryFull reportAbout validation report
History
DepositionAug 2, 2020-
Header (metadata) releaseOct 14, 2020-
Map releaseOct 14, 2020-
UpdateOct 14, 2020-
Current statusOct 14, 2020Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.025
  • Imaged by UCSF Chimera
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  • Surface view colored by radius
  • Surface level: 0.025
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6zym
  • Surface level: 0.05
  • Imaged by UCSF Chimera
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  • Simplified surface model + fitted atomic model
  • Atomic modelsPDB-6zym
  • Imaged by Jmol
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

Downloads & links

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Map

FileDownload / File: emd_11569.map.gz / Format: CCP4 / Size: 325 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.06 Å/pix.
x 440 pix.
= 466.4 Å
1.06 Å/pix.
x 440 pix.
= 466.4 Å
1.06 Å/pix.
x 440 pix.
= 466.4 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.06 Å
Density
Contour LevelBy AUTHOR: 0.025 / Movie #1: 0.025
Minimum - Maximum-0.18465073 - 0.33589733
Average (Standard dev.)-0.00011441692 (±0.008981588)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions440440440
Spacing440440440
CellA=B=C: 466.39996 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.061.061.06
M x/y/z440440440
origin x/y/z0.0000.0000.000
length x/y/z466.400466.400466.400
α/β/γ90.00090.00090.000
start NX/NY/NZ000
NX/NY/NZ161186271
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS440440440
D min/max/mean-0.1850.336-0.000

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Supplemental data

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Sample components

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Entire Human C complex Spliceosome - High resolution core

EntireName: Human C complex Spliceosome - High resolution core / Number of components: 30

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Component #1: protein, Human C complex Spliceosome - High resolution core

ProteinName: Human C complex Spliceosome - High resolution core / Recombinant expression: No
SourceSpecies: Homo sapiens (human) / Strain: HeLa S3

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Component #2: nucleic-acid, U2 snRNA

nucleic acidName: U2 snRNAU2 spliceosomal RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
AUCGCUUCUC GGCCUUUUGG CUAAGAUCAA GUGUAGUAUC UGUUCUUAUC AGUUUAAUAU CUGAUACGUC CUCUAUCCGA GGACAAUAUA UUAAAUGGAU UUUUGGAGCA GGGAGAUGGA AUAGGAGCUU GCUCCGUCCA CUCCACGCAU CGACCUGGUA UUGCAGUACC UCCAGGAACG GUGCACCC
MassTheoretical: 60.186445 kDa
SourceSpecies: Human (human)

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Component #3: nucleic-acid, U5 snRNA

nucleic acidName: U5 snRNAU5 spliceosomal RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
AUACUCUGGU UUCUCUUCAG AUCGCAUAAA UCUUUCGCCU UUUACUAAAG AUUUCCGUGG AGAGGAACAA CUCUGAGUCU UAACCCAAUU UUUUGAGCCU UGCCUUGGCA AGGCUA
MassTheoretical: 36.908668 kDa
SourceSpecies: Human (human)

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Component #4: nucleic-acid, U6 snRNA

nucleic acidName: U6 snRNAU6 spliceosomal RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
GUGCUCGCUU CGGCAGCACA UAUACUAAAA UUGGAACGAU ACAGAGAAGA UUAGCAUGGC CCCUGCGCAA GGAUGACAC
MassTheoretical: 25.48024 kDa
SourceSpecies: Human (human)

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Component #5: protein, Corepressor interacting with RBPJ 1

ProteinName: Corepressor interacting with RBPJ 1 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 52.44175 kDa
SourceSpecies: Human (human)

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Component #6: protein, Pre-mRNA-processing-splicing factor 8

ProteinName: Pre-mRNA-processing-splicing factor 8 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 207.470719 kDa
SourceSpecies: Human (human)

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Component #7: protein, 116 kDa U5 small nuclear ribonucleoprotein component

ProteinName: 116 kDa U5 small nuclear ribonucleoprotein component / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 107.225898 kDa
SourceSpecies: Human (human)

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Component #8: protein, SNW domain-containing protein 1

ProteinName: SNW domain-containing protein 1 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 61.610703 kDa
SourceSpecies: Human (human)

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Component #9: protein, Pleiotropic regulator 1

ProteinName: Pleiotropic regulator 1 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 57.280758 kDa
SourceSpecies: Human (human)

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Component #10: protein, Pre-mRNA-processing factor 17

ProteinName: Pre-mRNA-processing factor 17 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 65.61218 kDa
SourceSpecies: Human (human)

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Component #11: protein, U5 small nuclear ribonucleoprotein 40 kDa protein

ProteinName: U5 small nuclear ribonucleoprotein 40 kDa protein / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 39.359492 kDa
SourceSpecies: Human (human)

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Component #12: protein, Cell division cycle 5-like protein

ProteinName: Cell division cycle 5-like protein / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 92.406883 kDa
SourceSpecies: Human (human)

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Component #13: protein, Crooked neck-like protein 1

ProteinName: Crooked neck-like protein 1 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 100.610008 kDa
SourceSpecies: Human (human)

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Component #14: protein, Pre-mRNA-splicing factor RBM22

ProteinName: Pre-mRNA-splicing factor RBM22 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 25.223928 kDa
SourceSpecies: Human (human)

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Component #15: protein, Protein BUD31 homolog

ProteinName: Protein BUD31 homolog / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 17.03285 kDa
SourceSpecies: Human (human)

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Component #16: protein, Spliceosome-associated protein CWC15 homolog

ProteinName: Spliceosome-associated protein CWC15 homolog / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 26.674447 kDa
SourceSpecies: Human (human)

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Component #17: protein, Serine/arginine repetitive matrix protein 2

ProteinName: Serine/arginine repetitive matrix protein 2 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 300.255312 kDa
SourceSpecies: Human (human)

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Component #18: protein, Pre-mRNA-splicing factor CWC22 homolog

ProteinName: Pre-mRNA-splicing factor CWC22 homolog / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 105.646578 kDa
SourceSpecies: Human (human)

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Component #19: protein, Peptidyl-prolyl cis-trans isomerase-like 1

ProteinName: Peptidyl-prolyl cis-trans isomerase-like 1 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 18.257805 kDa
SourceSpecies: Human (human)

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Component #20: nucleic-acid, pre-mRNA

nucleic acidName: pre-mRNAPrimary transcript / Class: RNA / Structure: OTHER / Synthetic: No
Sequence: GGGAGACGGA AUUCGAGCUC GCCCACUCUU GGAUCGGAAA CCCGUCGGCC UCCGAACGGU AAGAGCCUAG CAUGUAGAAC UGGUUACCUG CAGCCCAAGC UUGCUGCACG UCUAGGGCGC AGUAGUCCAG GGUUUCCUUG AUGAUGUCAU ACUUAUCCUG UCCCUUUUUU ...Sequence:
GGGAGACGGA AUUCGAGCUC GCCCACUCUU GGAUCGGAAA CCCGUCGGCC UCCGAACGGU AAGAGCCUAG CAUGUAGAAC UGGUUACCUG CAGCCCAAGC UUGCUGCACG UCUAGGGCGC AGUAGUCCAG GGUUUCCUUG AUGAUGUCAU ACUUAUCCUG UCCCUUUUUU UUCCACAGCU CGCGGUUGAG GACAAACUCU UCGCGGUCUU UCCAGUGGGG AUCCGAUAUC CGUACACCAU CAGGGUACGA GCUAGCCCAU GGCGUACACC AUCAGGGUAC GACUAGUAGA UCUCGUACAC CAUCAGGGUA CGGAAUUCUC UAGA
MassTheoretical: 103.979148 kDa
SourceSpecies: Human (human)

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Component #21: protein, WD repeat-containing protein 70

ProteinName: WD repeat-containing protein 70 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 73.306172 kDa
SourceSpecies: Human (human)

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Component #22: protein, Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16

ProteinName: Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 140.720641 kDa
SourceSpecies: Human (human)

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Component #23: protein, Pre-mRNA-splicing factor ISY1 homolog

ProteinName: Pre-mRNA-splicing factor ISY1 homolog / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 33.046254 kDa
SourceSpecies: Human (human)

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Component #24: protein, Pre-mRNA-splicing factor CWC25 homolog

ProteinName: Pre-mRNA-splicing factor CWC25 homolog / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 49.769938 kDa
SourceSpecies: Human (human)

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Component #25: protein, Splicing factor YJU2

ProteinName: Splicing factor YJU2 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.452719 kDa
SourceSpecies: Human (human)

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Component #26: protein, Protein FRG1

ProteinName: Protein FRG1 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 29.227492 kDa
SourceSpecies: Human (human)

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Component #27: ligand, MAGNESIUM ION

LigandName: MAGNESIUM ION / Number of Copies: 5 / Recombinant expression: No
MassTheoretical: 2.430505 MDa

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Component #28: ligand, INOSITOL HEXAKISPHOSPHATE

LigandName: INOSITOL HEXAKISPHOSPHATEPhytic acid / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 0.660035 kDa

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Component #29: ligand, GUANOSINE-5'-TRIPHOSPHATE

LigandName: GUANOSINE-5'-TRIPHOSPHATEGuanosine triphosphate / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 0.52318 kDa

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Component #30: ligand, ZINC ION

LigandName: ZINC ION / Number of Copies: 7 / Recombinant expression: No
MassTheoretical: 6.540905 MDa

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Experimental details

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Sample preparation

SpecimenSpecimen state: Particle / Method: cryo EM
Sample solutionSpecimen conc.: 0.1 mg/mL / pH: 7.9
VitrificationInstrument: FEI VITROBOT MARK II / Cryogen name: ETHANE / Temperature: 277 K / Humidity: 100 %

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
ImagingMicroscope: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 120 e/Å2 / Illumination mode: SPOT SCAN
LensMagnification: 132000 X (nominal) / Cs: 2.7 mm / Imaging mode: BRIGHT FIELD / Defocus: -1.0 - -3.0 nm
Specimen HolderModel: FEI TITAN KRIOS AUTOGRID HOLDER
CameraDetector: FEI FALCON III (4k x 4k)

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Image acquisition

Image acquisitionNumber of digital images: 28279

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Image processing

ProcessingMethod: single particle reconstruction / Number of projections: 411539
3D reconstructionResolution: 3.4 Å / Resolution method: FSC 0.143 CUT-OFF

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Atomic model buiding

Output model

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