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- EMDB-11569: Human C Complex Spliceosome - High-resolution CORE -

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Basic information

Entry
Database: EMDB / ID: EMD-11569
TitleHuman C Complex Spliceosome - High-resolution CORE
Map data
Sample
  • Complex: Human C complex Spliceosome - High resolution core
    • RNA: x 4 types
    • Protein or peptide: x 21 types
  • Ligand: x 4 types
Keywordshuman C complex / spliceosome / hC / human step 1 spliceosome / SPLICING
Function / homology
Function and homology information


striated muscle dense body / post-spliceosomal complex / regulation of retinoic acid receptor signaling pathway / post-mRNA release spliceosomal complex / 3'-5' RNA helicase activity / generation of catalytic spliceosome for first transesterification step / regulation of vitamin D receptor signaling pathway / nuclear retinoic acid receptor binding / embryonic brain development / mRNA 3'-end processing ...striated muscle dense body / post-spliceosomal complex / regulation of retinoic acid receptor signaling pathway / post-mRNA release spliceosomal complex / 3'-5' RNA helicase activity / generation of catalytic spliceosome for first transesterification step / regulation of vitamin D receptor signaling pathway / nuclear retinoic acid receptor binding / embryonic brain development / mRNA 3'-end processing / RNA splicing, via transesterification reactions / C2H2 zinc finger domain binding / U2-type catalytic step 1 spliceosome / pre-mRNA binding / regulation of mRNA splicing, via spliceosome / positive regulation of mRNA splicing, via spliceosome / sno(s)RNA-containing ribonucleoprotein complex / host-mediated activation of viral transcription / U2-type precatalytic spliceosome / positive regulation of vitamin D receptor signaling pathway / mRNA cis splicing, via spliceosome / U2-type spliceosomal complex / nuclear vitamin D receptor binding / Notch binding / Transport of Mature mRNA derived from an Intron-Containing Transcript / U2-type catalytic step 2 spliceosome / Regulation of gene expression in late stage (branching morphogenesis) pancreatic bud precursor cells / RUNX3 regulates NOTCH signaling / NOTCH4 Intracellular Domain Regulates Transcription / positive regulation of neurogenesis / RNA Polymerase II Transcription Termination / NOTCH3 Intracellular Domain Regulates Transcription / WD40-repeat domain binding / protein peptidyl-prolyl isomerization / K63-linked polyubiquitin modification-dependent protein binding / nuclear androgen receptor binding / muscle organ development / generation of catalytic spliceosome for second transesterification step / Notch-HLH transcription pathway / Formation of paraxial mesoderm / positive regulation of transforming growth factor beta receptor signaling pathway / SMAD binding / spliceosomal complex assembly / mRNA Splicing - Minor Pathway / mRNA 3'-splice site recognition / negative regulation of DNA damage response, signal transduction by p53 class mediator / spliceosomal tri-snRNP complex assembly / Prp19 complex / U5 snRNA binding / U5 snRNP / intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / protein localization to nucleus / U2 snRNA binding / positive regulation of G1/S transition of mitotic cell cycle / U6 snRNA binding / pre-mRNA intronic binding / RNA processing / U1 snRNA binding / Cajal body / retinoic acid receptor signaling pathway / U4/U6 x U5 tri-snRNP complex / cellular response to retinoic acid / catalytic step 2 spliceosome / mRNA Splicing - Major Pathway / RNA splicing / DNA damage checkpoint signaling / positive regulation of RNA splicing / positive regulation of protein export from nucleus / nuclear receptor binding / response to cocaine / RNA polymerase II transcription regulatory region sequence-specific DNA binding / peptidylprolyl isomerase / peptidyl-prolyl cis-trans isomerase activity / spliceosomal complex / Downregulation of SMAD2/3:SMAD4 transcriptional activity / mRNA splicing, via spliceosome / NOTCH1 Intracellular Domain Regulates Transcription / positive regulation of protein import into nucleus / Transcription-Coupled Nucleotide Excision Repair (TC-NER) / Formation of TC-NER Pre-Incision Complex / Pre-NOTCH Transcription and Translation / Constitutive Signaling by NOTCH1 PEST Domain Mutants / Constitutive Signaling by NOTCH1 HD+PEST Domain Mutants / histone deacetylase binding / cellular response to xenobiotic stimulus / nuclear matrix / fibrillar center / Z disc / mRNA processing / Dual incision in TC-NER / Gap-filling DNA repair synthesis and ligation in TC-NER / rRNA processing / disordered domain specific binding / calcium-dependent protein binding / actin filament binding / cellular response to tumor necrosis factor / transcription corepressor activity / protein folding / site of double-strand break / cellular response to lipopolysaccharide
Similarity search - Function
Corepressor interacting with RBPJ 1 / : / Protein FRG1 / FRG1-like domain / CBF1-interacting co-repressor CIR, N-terminal domain / Pre-mRNA splicing factor / : / N-terminal domain of CBF1 interacting co-repressor CIR / Pre-mRNA splicing factor / N-terminal domain of CBF1 interacting co-repressor CIR ...Corepressor interacting with RBPJ 1 / : / Protein FRG1 / FRG1-like domain / CBF1-interacting co-repressor CIR, N-terminal domain / Pre-mRNA splicing factor / : / N-terminal domain of CBF1 interacting co-repressor CIR / Pre-mRNA splicing factor / N-terminal domain of CBF1 interacting co-repressor CIR / Splicing factor Yju2 / Saf4/Yju2 protein / Saf4/Yju2 protein / Pre-mRNA-splicing factor Isy1 / Pre-mRNA-splicing factor Isy1 superfamily / Isy1-like splicing family / Actin-crosslinking / : / Helix hairpin bin domain superfamily / : / : / mRNA splicing factor Cwf21 domain / cwf21 domain / Pre-mRNA-processing factor 17 / G10 protein signature 2. / Cyclophilin-type peptidyl-prolyl cis-trans isomerase, cyclophilin A-like / BUD31/G10-related, conserved site / G10 protein signature 1. / : / STL11, N-terminal / : / Pre-mRNA-splicing factor Syf1/CRNKL1 C-terminal HAT repeat / SKI-interacting protein SKIP, SNW domain / SKI-interacting protein, SKIP / SKIP/SNW domain / Pre-mRNA-splicing factor Cwf15/Cwc15 / Cwf15/Cwc15 cell cycle control protein / WD repeat Prp46/PLRG1-like / Pre-mRNA-splicing factor Cwc2/Slt11 / : / G10 protein / Pre-mRNA-splicing factor BUD31 / Pre-mRNA splicing factor component Cdc5p/Cef1, C-terminal / : / : / pre-mRNA splicing factor component / Initiation factor eIF-4 gamma, MA3 / MA3 domain / MI domain profile. / Domain in DAP-5, eIF4G, MA-3 and other proteins. / MIF4G domain / Myb-like DNA-binding domain / Middle domain of eukaryotic initiation factor 4G (eIF4G) / MIF4G-like, type 3 / Spt5 C-terminal domain / Spt5 C-terminal nonapeptide repeat binding Spt4 / Zinc finger, CCCH-type superfamily / zinc finger / 116kDa U5 small nuclear ribonucleoprotein component, N-terminal / 116kDa U5 small nuclear ribonucleoprotein component, C-terminal / Snu114, GTP-binding domain / 116 kDa U5 small nuclear ribonucleoprotein component N-terminus / Pre-mRNA-splicing factor Syf1-like / : / Helicase associated domain (HA2), ratchet-like / DEAD-box helicase, OB fold / Oligonucleotide/oligosaccharide-binding (OB)-fold / Helicase associated domain (HA2), winged-helix / Helicase-associated domain / Helicase associated domain (HA2) Add an annotation / Myb-type HTH DNA-binding domain profile. / Zinc finger, CCCH-type / Zinc finger C3H1-type profile. / HAT (Half-A-TPR) repeat / HAT (Half-A-TPR) repeats / Myb domain / Cyclophilin-type peptidyl-prolyl cis-trans isomerase / DNA/RNA helicase, ATP-dependent, DEAH-box type, conserved site / DEAH-box subfamily ATP-dependent helicases signature. / Elongation Factor G, domain II / Elongation Factor G, domain III / JAB1/Mov34/MPN/PAD-1 ubiquitin protease / Translation elongation factor EFG/EF2, domain IV / Elongation factor G, domain IV / Elongation factor G, domain IV / Elongation factor G C-terminus / Cyclophilin-type peptidyl-prolyl cis-trans isomerase, conserved site / Cyclophilin-type peptidyl-prolyl cis-trans isomerase signature. / Elongation factor EFG, domain V-like / Elongation factor G C-terminus / Cyclophilin-type peptidyl-prolyl cis-trans isomerase domain profile. / Cyclophilin-type peptidyl-prolyl cis-trans isomerase domain / Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD / Cyclophilin-like domain superfamily / EF-G domain III/V-like / PROCT domain / Prp8 RNase domain IV, fingers region / PROCT (NUC072) domain / PRO8NT domain / PROCN domain
Similarity search - Domain/homology
Pleiotropic regulator 1 / Pre-mRNA-processing factor 17 / Protein BUD31 homolog / SNW domain-containing protein 1 / Protein FRG1 / 116 kDa U5 small nuclear ribonucleoprotein component / Pre-mRNA-processing-splicing factor 8 / Corepressor interacting with RBPJ 1 / Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 / U5 small nuclear ribonucleoprotein 40 kDa protein ...Pleiotropic regulator 1 / Pre-mRNA-processing factor 17 / Protein BUD31 homolog / SNW domain-containing protein 1 / Protein FRG1 / 116 kDa U5 small nuclear ribonucleoprotein component / Pre-mRNA-processing-splicing factor 8 / Corepressor interacting with RBPJ 1 / Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 / U5 small nuclear ribonucleoprotein 40 kDa protein / Cell division cycle 5-like protein / Splicing factor YJU2 / Crooked neck-like protein 1 / Pre-mRNA-splicing factor CWC22 homolog / Pre-mRNA-splicing factor RBM22 / WD repeat-containing protein 70 / Pre-mRNA-splicing factor CWC25 homolog / Spliceosome-associated protein CWC15 homolog / Pre-mRNA-splicing factor ISY1 homolog / Serine/arginine repetitive matrix protein 2 / Peptidyl-prolyl cis-trans isomerase-like 1
Similarity search - Component
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.4 Å
AuthorsBertram K / Kastner B
Funding support Germany, 2 items
OrganizationGrant numberCountry
Max Planck Society Germany
German Research Foundation (DFG)SFB 860 Germany
CitationJournal: Mol Cell / Year: 2020
Title: Structural Insights into the Roles of Metazoan-Specific Splicing Factors in the Human Step 1 Spliceosome.
Authors: Karl Bertram / Leyla El Ayoubi / Olexandr Dybkov / Dmitry E Agafonov / Cindy L Will / Klaus Hartmuth / Henning Urlaub / Berthold Kastner / Holger Stark / Reinhard Lührmann /
Abstract: Human spliceosomes contain numerous proteins absent in yeast, whose functions remain largely unknown. Here we report a 3D cryo-EM structure of the human spliceosomal C complex at 3.4 Å core ...Human spliceosomes contain numerous proteins absent in yeast, whose functions remain largely unknown. Here we report a 3D cryo-EM structure of the human spliceosomal C complex at 3.4 Å core resolution and 4.5-5.7 Å at its periphery, and aided by protein crosslinking we determine its molecular architecture. Our structure provides additional insights into the spliceosome's architecture between the catalytic steps of splicing, and how proteins aid formation of the spliceosome's catalytically active RNP (ribonucleoprotein) conformation. It reveals the spatial organization of the metazoan-specific proteins PPWD1, WDR70, FRG1, and CIR1 in human C complexes, indicating they stabilize functionally important protein domains and RNA structures rearranged/repositioned during the B to C transition. Structural comparisons with human B, C, and P complexes reveal an intricate cascade of RNP rearrangements during splicing catalysis, with intermediate RNP conformations not found in yeast, and additionally elucidate the structural basis for the sequential recruitment of metazoan-specific spliceosomal proteins.
History
DepositionAug 2, 2020-
Header (metadata) releaseOct 14, 2020-
Map releaseOct 14, 2020-
UpdateOct 1, 2025-
Current statusOct 1, 2025Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.025
  • Imaged by UCSF Chimera
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  • Surface view colored by radius
  • Surface level: 0.025
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6zym
  • Surface level: 0.05
  • Imaged by UCSF Chimera
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  • Simplified surface model + fitted atomic model
  • Atomic modelsPDB-6zym
  • Imaged by Jmol
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_11569.png

Downloads & links

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Map

FileDownload / File: emd_11569.map.gz / Format: CCP4 / Size: 325 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.06 Å/pix.
x 440 pix.
= 466.4 Å		1.06 Å/pix.
x 440 pix.
= 466.4 Å		1.06 Å/pix.
x 440 pix.
= 466.4 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.06 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.025 / Movie #1: 0.025
Minimum - Maximum-0.18465073 - 0.33589733
Average (Standard dev.)-0.00011441692 (±0.008981588)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions440440440
Spacing440440440
CellA=B=C: 466.39996 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.061.061.06
M x/y/z440440440
origin x/y/z0.0000.0000.000
length x/y/z466.400466.400466.400
α/β/γ90.00090.00090.000
start NX/NY/NZ000
NX/NY/NZ161186271
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS440440440
D min/max/mean-0.1850.336-0.000

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Supplemental data

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Sample components

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Entire : Human C complex Spliceosome - High resolution core

EntireName: Human C complex Spliceosome - High resolution core
Components
  • Complex: Human C complex Spliceosome - High resolution core
    • RNA: U2 snRNA
    • RNA: U5 snRNA
    • RNA: U6 snRNA
    • Protein or peptide: Corepressor interacting with RBPJ 1
    • Protein or peptide: Pre-mRNA-processing-splicing factor 8
    • Protein or peptide: 116 kDa U5 small nuclear ribonucleoprotein component
    • Protein or peptide: SNW domain-containing protein 1
    • Protein or peptide: Pleiotropic regulator 1
    • Protein or peptide: Pre-mRNA-processing factor 17
    • Protein or peptide: U5 small nuclear ribonucleoprotein 40 kDa protein
    • Protein or peptide: Cell division cycle 5-like protein
    • Protein or peptide: Crooked neck-like protein 1
    • Protein or peptide: Pre-mRNA-splicing factor RBM22
    • Protein or peptide: Protein BUD31 homolog
    • Protein or peptide: Spliceosome-associated protein CWC15 homolog
    • Protein or peptide: Serine/arginine repetitive matrix protein 2
    • Protein or peptide: Pre-mRNA-splicing factor CWC22 homolog
    • Protein or peptide: Peptidyl-prolyl cis-trans isomerase-like 1
    • RNA: pre-mRNA
    • Protein or peptide: WD repeat-containing protein 70
    • Protein or peptide: Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16
    • Protein or peptide: Pre-mRNA-splicing factor ISY1 homolog
    • Protein or peptide: Pre-mRNA-splicing factor CWC25 homolog
    • Protein or peptide: Splicing factor YJU2
    • Protein or peptide: Protein FRG1
  • Ligand: MAGNESIUM ION
  • Ligand: INOSITOL HEXAKISPHOSPHATE
  • Ligand: GUANOSINE-5'-TRIPHOSPHATE
  • Ligand: ZINC ION

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Supramolecule #1: Human C complex Spliceosome - High resolution core

SupramoleculeName: Human C complex Spliceosome - High resolution core / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#25
Source (natural)Organism: Homo sapiens (human) / Strain: HeLa S3

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Macromolecule #1: U2 snRNA

MacromoleculeName: U2 snRNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 60.186445 KDa
SequenceString:
AUCGCUUCUC GGCCUUUUGG CUAAGAUCAA GUGUAGUAUC UGUUCUUAUC AGUUUAAUAU CUGAUACGUC CUCUAUCCGA GGACAAUAU AUUAAAUGGA UUUUUGGAGC AGGGAGAUGG AAUAGGAGCU UGCUCCGUCC ACUCCACGCA UCGACCUGGU A UUGCAGUA CCUCCAGGAA CGGUGCACCC

GENBANK: GENBANK: X02665.1

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Macromolecule #2: U5 snRNA

MacromoleculeName: U5 snRNA / type: rna / ID: 2 / Number of copies: 1
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 36.908668 KDa
SequenceString:
AUACUCUGGU UUCUCUUCAG AUCGCAUAAA UCUUUCGCCU UUUACUAAAG AUUUCCGUGG AGAGGAACAA CUCUGAGUCU UAACCCAAU UUUUUGAGCC UUGCCUUGGC AAGGCUA

GENBANK: GENBANK: X01691.1

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Macromolecule #3: U6 snRNA

MacromoleculeName: U6 snRNA / type: rna / ID: 3 / Number of copies: 1
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 25.48024 KDa
SequenceString:
GUGCUCGCUU CGGCAGCACA UAUACUAAAA UUGGAACGAU ACAGAGAAGA UUAGCAUGGC CCCUGCGCAA GGAUGACAC

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Macromolecule #19: pre-mRNA

MacromoleculeName: pre-mRNA / type: rna / ID: 19 / Number of copies: 2
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 103.979148 KDa
SequenceString: GGGAGACGGA AUUCGAGCUC GCCCACUCUU GGAUCGGAAA CCCGUCGGCC UCCGAACGGU AAGAGCCUAG CAUGUAGAAC UGGUUACCU GCAGCCCAAG CUUGCUGCAC GUCUAGGGCG CAGUAGUCCA GGGUUUCCUU GAUGAUGUCA UACUUAUCCU G UCCCUUUU ...String:
GGGAGACGGA AUUCGAGCUC GCCCACUCUU GGAUCGGAAA CCCGUCGGCC UCCGAACGGU AAGAGCCUAG CAUGUAGAAC UGGUUACCU GCAGCCCAAG CUUGCUGCAC GUCUAGGGCG CAGUAGUCCA GGGUUUCCUU GAUGAUGUCA UACUUAUCCU G UCCCUUUU UUUUCCACAG CUCGCGGUUG AGGACAAACU CUUCGCGGUC UUUCCAGUGG GGAUCCGAUA UCCGUACACC AU CAGGGUA CGAGCUAGCC CAUGGCGUAC ACCAUCAGGG UACGACUAGU AGAUCUCGUA CACCAUCAGG GUACGGAAUU CUC UAGA

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Macromolecule #4: Corepressor interacting with RBPJ 1

MacromoleculeName: Corepressor interacting with RBPJ 1 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 52.44175 KDa
SequenceString: MGKSFANFMC KKDFHPASKS NIKKVWMAEQ KISYDKKKQE ELMQQYLKEQ ESYDNRLLMG DERVKNGLNF MYEAPPGAKK ENKEKEETE GETEYKFEWQ KGAPREKYAK DDMNIRDQPF GIQVRNVRCI KCHKWGHVNT DRECPLFGLS GINASSVPTD G SGPSMHPS ...String:
MGKSFANFMC KKDFHPASKS NIKKVWMAEQ KISYDKKKQE ELMQQYLKEQ ESYDNRLLMG DERVKNGLNF MYEAPPGAKK ENKEKEETE GETEYKFEWQ KGAPREKYAK DDMNIRDQPF GIQVRNVRCI KCHKWGHVNT DRECPLFGLS GINASSVPTD G SGPSMHPS ELIAEMRNSG FALKRNVLGR NLTANDPSQE YVASEGEEDP EVEFLKSLTT KQKQKLLRKL DRLEKKKKKK DR KKKKFQK SRSKHKKHKS SSSSSSSSSS SSSTETSESS SESESNNKEK KIQRKKRKKN KCSGHNNSDS EEKDKSKKRK LHE ELSSSH HNREKAKEKP RFLKHESSRE DSKWSHSDSD KKSRTHKHSP EKRGSERKEG SSRSHGREER SRRSRSRSPG SYKQ RETRK RAQRNPGEEQ SRRNDSRSHG TDLYRGEKMY REHPGGTHTK VTQRE

UniProtKB: Corepressor interacting with RBPJ 1

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Macromolecule #5: Pre-mRNA-processing-splicing factor 8

MacromoleculeName: Pre-mRNA-processing-splicing factor 8 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 207.470719 KDa
SequenceString: MAGVFPYRGP GNPVPGPLAP LPDYMSEEKL QEKARKWQQL QAKRYAEKRK FGFVDAQKED MPPEHVRKII RDHGDMTNRK FRHDKRVYL GALKYMPHAV LKLLENMPMP WEQIRDVPVL YHITGAISFV NEIPWVIEPV YISQWGSMWI MMRREKRDRR H FKRMRFPP ...String:
MAGVFPYRGP GNPVPGPLAP LPDYMSEEKL QEKARKWQQL QAKRYAEKRK FGFVDAQKED MPPEHVRKII RDHGDMTNRK FRHDKRVYL GALKYMPHAV LKLLENMPMP WEQIRDVPVL YHITGAISFV NEIPWVIEPV YISQWGSMWI MMRREKRDRR H FKRMRFPP FDDEEPPLDY ADNILDVEPL EAIQLELDPE EDAPVLDWFY DHQPLRDSRK YVNGSTYQRW QFTLPMMSTL YR LANQLLT DLVDDNYFYL FDLKAFFTSK ALNMAIPGGP KFEPLVRDIN LQDEDWNEFN DINKIIIRQP IRTEYKIAFP YLY NNLPHH VHLTWYHTPN VVFIKTEDPD LPAFYFDPLI NPISHRHSVK SQEPLPDDDE EFELPEFVEP FLKDTPLYTD NTAN GIALL WAPRPFNLRS GRTRRALDIP LVKNWYREHC PAGQPVKVRV SYQKLLKYYV LNALKHRPPK AQKKRYLFRS FKATK FFQS TKLDWVEVGL QVCRQGYNML NLLIHRKNLN YLHLDYNFNL KPVKTLTTKE RKKSRFGNAF HLCREVLRLT KLVVDS HVQ YRLGNVDAFQ LADGLQYIFA HVGQLTGMYR YKYKLMRQIR MCKDLKHLIY YRFNTGPVGK GPGCGFWAAG WRVWLFF MR GITPLLERWL GNLLARQFEG RHSKGVAKTV TKQRVESHFD LELRAAVMHD ILDMMPEGIK QNKARTILQH LSEAWRCW K ANIPWKVPGL PTPIENMILR YVKAKADWWT NTAHYNRERI RRGATVDKTV CKKNLGRLTR LYLKAEQERQ HNYLKDGPY ITAEEAVAVY TTTVHWLESR RFSPIPFPPL SYKHDTKLLI LALERLKEAY SVKSRLNQSQ REELGLIEQA YDNPHEALSR IKRHLLTQR AFKEVGIEFM DLYSHLVPVY DVEPLEKITD AYLDQYLWYE ADKRRLFPPW IKPADTEPPP LLVYKWCQGI N NLQDVWET SEGECNVMLE SRFEKMYEKI DLTLLNRLLR LIVDHNIADY MTAKNNVVIN YKDMNHTNSY GIIRGLQFAS FI VQYYGLV MDLLVLGLHR ASEMAGPPQM PNDFLSFQDI ATEAAHPIRL FCRYIDRIHI FFRFTADEAR DLIQRYLTEH PDP NNENIV GYNNKKCWPR DARMRLMKHD VNLGRAVFWD IKNRLPRSVT TVQWENSFVS VYSKDNPNLL FNMCGFECRI LPKC RTSYE EFTHKDGVWN LQNEVTKERT AQCFLRVDDE SMQRFHNRVR QILMASGSTT FTKIVNKWNT ALIGLMTYFR EAVVN TQEL LDLLVKCENK IQTRIKIGLN SKMPSRFPPV VFYTPKELGG LGMLSMGHVL IPQSDLRWSK QTDVGITHFR SGMSHE EDQ LIPNLYRYIQ PWESEFIDSQ RVWAEYALKR QEAIAQNRRL TLEDLEDSWD RGIPRINTLF QKDRHTLAYD KGWRVRT DF KQYQVLKQNP FWWTHQRHDG KLWNLNNYRT DMIQALGGVE GILEHTLFKG TYFPTWEGLF WEKASGFEES MKWKKLTN A QRSGLNQIPN RRFTLWWSPT INRANVYVGF QVQLDLTGIF MHGKIPTLKI SLIQIFRAHL WQKIHESIVM DLCQVFDQE LDALEIETVQ KETIHPRKSY KMNSSCADIL LFASYKWNVS RPSLLADSKD VMDSTTTQKY WIDIQLRWGD YDSHDIERYA RAKFLDYTT DNMSIYPSPT GVLIAIDLAY NLHSAYGNWF PGSKPLIQQA MAKIMKANPA LYVLRERIRK GLQLYS

UniProtKB: Pre-mRNA-processing-splicing factor 8

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Macromolecule #6: 116 kDa U5 small nuclear ribonucleoprotein component

MacromoleculeName: 116 kDa U5 small nuclear ribonucleoprotein component / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 107.225898 KDa
SequenceString: MDTDLYDEFG NYIGPELDSD EDDDELGRET KDLDEMDDDD DDDDVGDHDD DHPGMEVVLH EDKKYYPTAE EVYGPEVETI VQEEDTQPL TEPIIKPVKT KKFTLMEQTL PVTVYEMDFL ADLMDNSELI RNVTLCGHLH HGKTCFVDCL IEQTHPEIRK R YDQDLCYT ...String:
MDTDLYDEFG NYIGPELDSD EDDDELGRET KDLDEMDDDD DDDDVGDHDD DHPGMEVVLH EDKKYYPTAE EVYGPEVETI VQEEDTQPL TEPIIKPVKT KKFTLMEQTL PVTVYEMDFL ADLMDNSELI RNVTLCGHLH HGKTCFVDCL IEQTHPEIRK R YDQDLCYT DILFTEQERG VGIKSTPVTV VLPDTKGKSY LFNIMDTPGH VNFSDEVTAG LRISDGVVLF IDAAEGVMLN TE RLIKHAV QERLAVTVCI NKIDRLILEL KLPPTDAYYK LRHIVDEVNG LISMYSTDEN LILSPLLGNV CFSSSQYSIC FTL GSFAKI YADTFGDINY QEFAKRLWGD IYFNPKTRKF TKKAPTSSSQ RSFVEFILEP LYKILAQVVG DVDTSLPRTL DELG IHLTK EELKLNIRPL LRLVCKKFFG EFTGFVDMCV QHIPSPKVGA KPKIEHTYTG GVDSDLGEAM SDCDPDGPLM CHTTK MYST DDGVQFHAFG RVLSGTIHAG QPVKVLGENY TLEDEEDSQI CTVGRLWISV ARYHIEVNRV PAGNWVLIEG VDQPIV KTA TITEPRGNEE AQIFRPLKFN TTSVIKIAVE PVNPSELPKM LDGLRKVNKS YPSLTTKVEE SGEHVILGTG ELYLDCV MH DLRKMYSEID IKVADPVVTF CETVVETSSL KCFAETPNKK NKITMIAEPL EKGLAEDIEN EVVQITWNRK KLGEFFQT K YDWDLLAARS IWAFGPDATG PNILVDDTLP SEVDKALLGS VKDSIVQGFQ WGTREGPLCD ELIRNVKFKI LDAVVAQEP LHRGGGQIIP TARRVVYSAF LMATPRLMEP YYFVEVQAPA DCVSAVYTVL ARRRGHVTQD APIPGSPLYT IKAFIPAIDS FGFETDLRT HTQGQAFSLS VFHHWQIVPG DPLDKSIVIR PLEPQPAPHL AREFMIKTRR RKGLSEDVSI SKF

UniProtKB: 116 kDa U5 small nuclear ribonucleoprotein component

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Macromolecule #7: SNW domain-containing protein 1

MacromoleculeName: SNW domain-containing protein 1 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 61.610703 KDa
SequenceString: MALTSFLPAP TQLSQDQLEA EEKARSQRSR QTSLVSSRRE PPPYGYRKGW IPRLLEDFGD GGAFPEIHVA QYPLDMGRKK KMSNALAIQ VDSEGKIKYD AIARQGQSKD KVIYSKYTDL VPKEVMNADD PDLQRPDEEA IKEITEKTRV ALEKSVSQKV A AAMPVRAA ...String:
MALTSFLPAP TQLSQDQLEA EEKARSQRSR QTSLVSSRRE PPPYGYRKGW IPRLLEDFGD GGAFPEIHVA QYPLDMGRKK KMSNALAIQ VDSEGKIKYD AIARQGQSKD KVIYSKYTDL VPKEVMNADD PDLQRPDEEA IKEITEKTRV ALEKSVSQKV A AAMPVRAA DKLAPAQYIR YTPSQQGVAF NSGAKQRVIR MVEMQKDPME PPRFKINKKI PRGPPSPPAP VMHSPSRKMT VK EQQEWKI PPCISNWKNA KGYTIPLDKR LAADGRGLQT VHINENFAKL AEALYIADRK AREAVEMRAQ VERKMAQKEK EKH EEKLRE MAQKARERRA GIKTHVEKED GEARERDEIR HDRRKERQHD RNLSRAAPDK RSKLQRNENR DISEVIALGV PNPR TSNEV QYDQRLFNQS KGMDSGFAGG EDEIYNVYDQ AWRGGKDMAQ SIYRPSKNLD KDMYGDDLEA RIKTNRFVPD KEFSG SDRR QRGREGPVQF EEDPFGLDKF LEEAKQHGGS KRPSDSSRPK EHEHEGKKRR KE

UniProtKB: SNW domain-containing protein 1

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Macromolecule #8: Pleiotropic regulator 1

MacromoleculeName: Pleiotropic regulator 1 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 57.280758 KDa
SequenceString: MVEEVQKHSV HTLVFRSLKR THDMFVADNG KPVPLDEESH KRKMAIKLRN EYGPVLHMPT SKENLKEKGP QNATDSYVHK QYPANQGQE VEYFVAGTHP YPPGPGVALT ADTKIQRMPS ESAAQSLAVA LPLQTKADAN RTAPSGSEYR HPGASDRPQP T AMNSIVME ...String:
MVEEVQKHSV HTLVFRSLKR THDMFVADNG KPVPLDEESH KRKMAIKLRN EYGPVLHMPT SKENLKEKGP QNATDSYVHK QYPANQGQE VEYFVAGTHP YPPGPGVALT ADTKIQRMPS ESAAQSLAVA LPLQTKADAN RTAPSGSEYR HPGASDRPQP T AMNSIVME TGNTKNSALM AKKAPTMPKP QWHPPWKLYR VISGHLGWVR CIAVEPGNQW FVTGSADRTI KIWDLASGKL KL SLTGHIS TVRGVIVSTR SPYLFSCGED KQVKCWDLEY NKVIRHYHGH LSAVYGLDLH PTIDVLVTCS RDSTARIWDV RTK ASVHTL SGHTNAVATV RCQAAEPQII TGSHDTTIRL WDLVAGKTRV TLTNHKKSVR AVVLHPRHYT FASGSPDNIK QWKF PDGSF IQNLSGHNAI INTLTVNSDG VLVSGADNGT MHLWDWRTGY NFQRVHAAVQ PGSLDSESGI FACAFDQSES RLLTA EADK TIKVYREDDT ATEETHPVSW KPEIIKRKRF

UniProtKB: Pleiotropic regulator 1

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Macromolecule #9: Pre-mRNA-processing factor 17

MacromoleculeName: Pre-mRNA-processing factor 17 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 65.61218 KDa
SequenceString: MSAAIAALAA SYGSGSGSES DSDSESSRCP LPAADSLMHL TKSPSSKPSL AVAVDSAPEV AVKEDLETGV HLDPAVKEVQ YNPTYETMF APEFGPENPF RTQQMAAPRN MLSGYAEPAH INDFMFEQQR RTFATYGYAL DPSLDNHQVS AKYIGSVEEA E KNQGLTVF ...String:
MSAAIAALAA SYGSGSGSES DSDSESSRCP LPAADSLMHL TKSPSSKPSL AVAVDSAPEV AVKEDLETGV HLDPAVKEVQ YNPTYETMF APEFGPENPF RTQQMAAPRN MLSGYAEPAH INDFMFEQQR RTFATYGYAL DPSLDNHQVS AKYIGSVEEA E KNQGLTVF ETGQKKTEKR KKFKENDASN IDGFLGPWAK YVDEKDVAKP SEEEQKELDE ITAKRQKKGK QEEEKPGEEK TI LHVKEMY DYQGRSYLHI PQDVGVNLRS TMPPEKCYLP KKQIHVWSGH TKGVSAVRLF PLSGHLLLSC SMDCKIKLWE VYG ERRCLR TFIGHSKAVR DICFNTAGTQ FLSAAYDRYL KLWDTETGQC ISRFTNRKVP YCVKFNPDED KQNLFVAGMS DKKI VQWDI RSGEIVQEYD RHLGAVNTIV FVDENRRFVS TSDDKSLRVW EWDIPVDFKY IAEPSMHSMP AVTLSPNGKW LACQS MDNQ ILIFGAQNRF RLNKKKIFKG HMVAGYACQV DFSPDMSYVI SGDGNGKLNI WDWKTTKLYS RFKAHDKVCI GAVWHP HET SKVITCGWDG LIKLWD

UniProtKB: Pre-mRNA-processing factor 17

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Macromolecule #10: U5 small nuclear ribonucleoprotein 40 kDa protein

MacromoleculeName: U5 small nuclear ribonucleoprotein 40 kDa protein / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 39.359492 KDa
SequenceString: MIEQQKRKGP ELPLVPVKRQ RHELLLGAGS GPGAGQQQAT PGALLQAGPP RCSSLQAPIM LLSGHEGEVY CCKFHPNGST LASAGFDRL ILLWNVYGDC DNYATLKGHS GAVMELHYNT DGSMLFSAST DKTVAVWDSE TGERVKRLKG HTSFVNSCYP A RRGPQLVC ...String:
MIEQQKRKGP ELPLVPVKRQ RHELLLGAGS GPGAGQQQAT PGALLQAGPP RCSSLQAPIM LLSGHEGEVY CCKFHPNGST LASAGFDRL ILLWNVYGDC DNYATLKGHS GAVMELHYNT DGSMLFSAST DKTVAVWDSE TGERVKRLKG HTSFVNSCYP A RRGPQLVC TGSDDGTVKL WDIRKKAAIQ TFQNTYQVLA VTFNDTSDQI ISGGIDNDIK VWDLRQNKLT YTMRGHADSV TG LSLSSEG SYLLSNAMDN TVRVWDVRPF APKERCVKIF QGNVHNFEKN LLRCSWSPDG SKIAAGSADR FVYVWDTTSR RIL YKLPGH AGSINEVAFH PDEPIIISAS SDKRLYMGEI Q

UniProtKB: U5 small nuclear ribonucleoprotein 40 kDa protein

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Macromolecule #11: Cell division cycle 5-like protein

MacromoleculeName: Cell division cycle 5-like protein / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 92.406883 KDa
SequenceString: MPRIMIKGGV WRNTEDEILK AAVMKYGKNQ WSRIASLLHR KSAKQCKARW YEWLDPSIKK TEWSREEEEK LLHLAKLMPT QWRTIAPII GRTAAQCLEH YEFLLDKAAQ RDNEEETTDD PRKLKPGEID PNPETKPARP DPIDMDEDEL EMLSEARARL A NTQGKKAK ...String:
MPRIMIKGGV WRNTEDEILK AAVMKYGKNQ WSRIASLLHR KSAKQCKARW YEWLDPSIKK TEWSREEEEK LLHLAKLMPT QWRTIAPII GRTAAQCLEH YEFLLDKAAQ RDNEEETTDD PRKLKPGEID PNPETKPARP DPIDMDEDEL EMLSEARARL A NTQGKKAK RKAREKQLEE ARRLAALQKR RELRAAGIEI QKKRKRKRGV DYNAEIPFEK KPALGFYDTS EENYQALDAD FR KLRQQDL DGELRSEKEG RDRKKDKQHL KRKKESDLPS AILQTSGVSE FTKKRSKLVL PAPQISDAEL QEVVKVGQAS EIA RQTAEE SGITNSASST LLSEYNVTNN SVALRTPRTP ASQDRILQEA QNLMALTNVD TPLKGGLNTP LHESDFSGVT PQRQ VVQTP NTVLSTPFRT PSNGAEGLTP RSGTTPKPVI NSTPGRTPLR DKLNINPEDG MADYSDPSYV KQMERESREH LRLGL LGLP APKNDFEIVL PENAEKELEE REIDDTYIED AADVDARKQA IRDAERVKEM KRMHKAVQKD LPRPSEVNET ILRPLN VEP PLTDLQKSEE LIKKEMITML HYDLLHHPYE PSGNKKGKTV GFGTNNSEHI TYLEHNPYEK FSKEELKKAQ DVLVQEM EV VKQGMSHGEL SSEAYNQVWE ECYSQVLYLP GQSRYTRANL ASKKDRIESL EKRLEINRGH MTTEAKRAAK MEKKMKIL L GGYQSRAMGL MKQLNDLWDQ IEQAHLELRT FEELKKHEDS AIPRRLECLK EDVQRQQERE KELQHRYADL LLEKETLKS KF

UniProtKB: Cell division cycle 5-like protein

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Macromolecule #12: Crooked neck-like protein 1

MacromoleculeName: Crooked neck-like protein 1 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 100.610008 KDa
SequenceString: MTATVENLTF QKDTLGNAVD KNTSRLELRS YSLAGRHGST EPLVLAWSSQ FRRLTWGCAL DALHRSPCVA ASQHGVTHLI RSSRTPHST RCRKEDAQPG HHGNGAASVT AQARGQRSVL QVPLPVPRSC LFSESFVVSV SSQSRFLASV PGTGVQRSTA A DMAASTAA ...String:
MTATVENLTF QKDTLGNAVD KNTSRLELRS YSLAGRHGST EPLVLAWSSQ FRRLTWGCAL DALHRSPCVA ASQHGVTHLI RSSRTPHST RCRKEDAQPG HHGNGAASVT AQARGQRSVL QVPLPVPRSC LFSESFVVSV SSQSRFLASV PGTGVQRSTA A DMAASTAA GKQRIPKVAK VKNKAPAEVQ ITAEQLLREA KERELELLPP PPQQKITDEE ELNDYKLRKR KTFEDNIRKN RT VISNWIK YAQWEESLKE IQRARSIYER ALDVDYRNIT LWLKYAEMEM KNRQVNHARN IWDRAITTLP RVNQFWYKYT YME EMLGNV AGARQVFERW MEWQPEEQAW HSYINFELRY KEVDRARTIY ERFVLVHPDV KNWIKYARFE EKHAYFAHAR KVYE RAVEF FGDEHMDEHL YVAFAKFEEN QKEFERVRVI YKYALDRISK QDAQELFKNY TIFEKKFGDR RGIEDIIVSK RRFQY EEEV KANPHNYDAW FDYLRLVESD AEAEAVREVY ERAIANVPPI QEKRHWKRYI YLWINYALYE ELEAKDPERT RQVYQA SLE LIPHKKFTFA KMWILYAQFE IRQKNLSLAR RALGTSIGKC PKNKLFKVYI ELELQLREFD RCRKLYEKFL EFGPENC TS WIKFAELETI LGDIDRARAI YELAISQPRL DMPEVLWKSY IDFEIEQEET ERTRNLYRRL LQRTQHVKVW ISFAQFEL S SGKEGSLTKC RQIYEEANKT MRNCEEKEER LMLLESWRSF EEEFGTASDK ERVDKLMPEK VKKRRKVQTD DGSDAGWEE YFDYIFPEDA ANQPNLKLLA MAKLWKKQQQ EKEDAEHHPD EDVDESES

UniProtKB: Crooked neck-like protein 1

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Macromolecule #13: Pre-mRNA-splicing factor RBM22

MacromoleculeName: Pre-mRNA-splicing factor RBM22 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 25.223928 KDa
SequenceString: MATSLGSNTY NRQNWEDADF PILCQTCLGE NPYIRMTKEK YGKECKICAR PFTVFRWCPG VRMRFKKTEV CQTCSKLKNV CQTCLLDLE YGLPIQVRDA GLSFKDDMPK SDVNKEYYTQ NMEREISNSD GTRPVGMLGK ATSTSDMLLK LARTTPYYKR N RPHICSFW ...String:
MATSLGSNTY NRQNWEDADF PILCQTCLGE NPYIRMTKEK YGKECKICAR PFTVFRWCPG VRMRFKKTEV CQTCSKLKNV CQTCLLDLE YGLPIQVRDA GLSFKDDMPK SDVNKEYYTQ NMEREISNSD GTRPVGMLGK ATSTSDMLLK LARTTPYYKR N RPHICSFW VKGECKRGEE CPYRHEKPTD PDDPLADQNI KDRYYGINDP VADKLLKRAS

UniProtKB: Pre-mRNA-splicing factor RBM22

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Macromolecule #14: Protein BUD31 homolog

MacromoleculeName: Protein BUD31 homolog / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 17.03285 KDa
SequenceString:
MPKVKRSRKA PPDGWELIEP TLDELDQKMR EAETEPHEGK RKVESLWPIF RIHHQKTRYI FDLFYKRKAI SRELYEYCIK EGYADKNLI AKWKKQGYEN LCCLRCIQTR DTNFGTNCIC RVPKSKLEVG RIIECTHCGC RGCSG

UniProtKB: Protein BUD31 homolog

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Macromolecule #15: Spliceosome-associated protein CWC15 homolog

MacromoleculeName: Spliceosome-associated protein CWC15 homolog / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 26.674447 KDa
SequenceString: MTTAARPTFE PARGGRGKGE GDLSQLSKQY SSRDLPSHTK IKYRQTTQDA PEEVRNRDFR RELEERERAA AREKNRDRPT REHTTSSSV SKKPRLDQIP AANLDADDPL TDEEDEDFEE ESDDDDTAAL LAELEKIKKE RAEEQARKEQ EQKAEEERIR M ENILSGNP ...String:
MTTAARPTFE PARGGRGKGE GDLSQLSKQY SSRDLPSHTK IKYRQTTQDA PEEVRNRDFR RELEERERAA AREKNRDRPT REHTTSSSV SKKPRLDQIP AANLDADDPL TDEEDEDFEE ESDDDDTAAL LAELEKIKKE RAEEQARKEQ EQKAEEERIR M ENILSGNP LLNLTGPSQP QANFKVKRRW DDDVVFKNCA KGVDDQKKDK RFVNDTLRSE FHKKFMEKYI K

UniProtKB: Spliceosome-associated protein CWC15 homolog

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Macromolecule #16: Serine/arginine repetitive matrix protein 2

MacromoleculeName: Serine/arginine repetitive matrix protein 2 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 300.255312 KDa
SequenceString: MYNGIGLPTP RGSGTNGYVQ RNLSLVRGRR GERPDYKGEE ELRRLEAALV KRPNPDILDH ERKRRVELRC LELEEMMEEQ GYEEQQIQE KVATFRLMLL EKDVNPGGKE ETPGQRPAVT ETHQLAELNE KKNERLRAAF GISDSYVDGS SFDPQRRARE A KQPAPEPP ...String:
MYNGIGLPTP RGSGTNGYVQ RNLSLVRGRR GERPDYKGEE ELRRLEAALV KRPNPDILDH ERKRRVELRC LELEEMMEEQ GYEEQQIQE KVATFRLMLL EKDVNPGGKE ETPGQRPAVT ETHQLAELNE KKNERLRAAF GISDSYVDGS SFDPQRRARE A KQPAPEPP KPYSLVRESS SSRSPTPKQK KKKKKKDRGR RSESSSPRRE RKKSSKKKKH RSESESKKRK HRSPTPKSKR KS KDKKRKR SRSTTPAPKS RRAHRSTSAD SASSSDTSRS RSRSAAAKTH TTALAGRSPS PASGRRGEGD APFSEPGTTS TQR PSSPET ATKQPSSPYE DKDKDKKEKS ATRPSPSPER SSTGPEPPAP TPLLAERHGG SPQPLATTPL SQEPVNPPSE ASPT RDRSP PKSPEKLPQS SSSESSPPSP QPTKVSRHAS SSPESPKPAP APGSHREISS SPTSKNRSHG RAKRDKSHSH TPSRR MGRS RSPATAKRGR SRSRTPTKRG HSRSRSPQWR RSRSAQRWGR SRSPQRRGRS RSPQRPGWSR SRNTQRRGRS RSARRG RSH SRSPATRGRS RSRTPARRGR SRSRTPARRR SRSRTPTRRR SRSRTPARRG RSRSRTPARR RSRTRSPVRR RSRSRSP AR RSGRSRSRTP ARRGRSRSRT PARRGRSRSR TPARRSGRSR SRTPARRGRS RSRTPRRGRS RSRSLVRRGR SHSRTPQR R GRSGSSSERK NKSRTSQRRS RSNSSPEMKK SRISSRRSRS LSSPRSKAKS RLSLRRSLSG SSPCPKQKSQ TPPRRSRSG SSQPKAKSRT PPRRSRSSSS PPPKQKSKTP SRQSHSSSSP HPKVKSGTPP RQGSITSPQA NEQSVTPQRR SCFESSPDPE LKSRTPSRH SCSGSSPPRV KSSTPPRQSP SRSSSPQPKV KAIISPRQRS HSGSSSPSPS RVTSRTTPRR SRSVSPCSNV E SRLLPRYS HSGSSSPDTK VKPETPPRQS HSGSISPYPK VKAQTPPGPS LSGSKSPCPQ EKSKDSLVQS CPGSLSLCAG VK SSTPPGE SYFGVSSLQL KGQSQTSPDH RSDTSSPEVR QSHSESPSLQ SKSQTSPKGG RSRSSSPVTE LASRSPIRQD RGE FSASPM LKSGMSPEQS RFQSDSSSYP TVDSNSLLGQ SRLETAESKE KMALPPQEDA TASPPRQKDK FSPFPVQDRP ESSL VFKDT LRTPPRERSG AGSSPETKEQ NSALPTSSQD EELMEVVEKS EEPAGQILSH LSSELKEMST SNFESSPEVE ERPAV SLTL DQSQSQASLE AVEVPSMASS WGGPHFSPEH KELSNSPLRE NSFGSPLEFR NSGPLGTEMN TGFSSEVKED LNGPFL NQL ETDPSLDMKE QSTRSSGHSS SELSPDAVEK AGMSSNQSIS SPVLDAVPRT PSRERSSSAS SPEMKDGLPR TPSRRSR SG SSPGLRDGSG TPSRHSLSGS SPGMKDIPRT PSRGRSECDS SPEPKALPQT PRPRSRSPSS PELNNKCLTP QRERSGSE S SVDQKTVART PLGQRSRSGS SQELDVKPSA SPQERSESDS SPDSKAKTRT PLRQRSRSGS SPEVDSKSRL SPRRSRSGS SPEVKDKPRA APRAQSGSDS SPEPKAPAPR ALPRRSRSGS SSKGRGPSPE GSSSTESSPE HPPKSRTARR GSRSSPEPKT KSRTPPRRR SSRSSPELTR KARLSRRSRS ASSSPETRSR TPPRHRRSPS VSSPEPAEKS RSSRRRRSAS SPRTKTTSRR G RSPSPKPR GLQRSRSRSR REKTRTTRRR DRSGSSQSTS RRRQRSRSRS RVTRRRRGGS GYHSRSPARQ ESSRTSSRRR RG RSRTPPT SRKRSRSRTS PAPWKRSRSR ASPATHRRSR SRTPLISRRR SRSRTSPVSR RRSRSRTSVT RRRSRSRASP VSR RRSRSR TPPVTRRRSR SRTPTTRRRS RSRTPPVTRR RSRSRTPPVT RRRSRSRTSP ITRRRSRSRT SPVTRRRSRS RTSP VTRRR SRSRTSPVTR RRSRSRTPPA IRRRSRSRTP LLPRKRSRSR SPLAIRRRSR SRTPRTARGK RSLTRSPPAI RRRSA SGSS SDRSRSATPP ATRNHSGSRT PPVALNSSRM SCFSRPSMSP TPLDRCRSPG MLEPLGSSRT PMSVLQQAGG SMMDGP GPR IPDHQRTSVP ENHAQSRIAL ALTAISLGTA RPPPSMSAAG LAARMSQVPA PVPLMSLRTA PAANLASRIP AASAAAM NL ASARTPAIPT AVNLADSRTP AAAAAMNLAS PRTAVAPSAV NLADPRTPTA PAVNLAGART PAALAALSLT GSGTPPTA A NYPSSSRTPQ APASANLVGP RSAHATAPVN IAGSRTAAAL APASLTSARM APALSGANLT SPRVPLSAYE RVSGRTSPP LLDRARSRTP PSAPSQSRMT SERAPSPSSR MGQAPSQSLL PPAQDQPRSP VPSAFSDQSR CLIAQTTPVA GSQSLSSGAV ATTTSSAGD HNGMLSVPAP GVPHSDVGEP PASTGAQQPS ALAALQPAKE RRSSSSSSSS SSSSSSSSSS SSSSSSSGSS S SDSEGSSL PVQPEVALKR VPSPTPAPKE AVREGRPPEP TPAKRKRRSS SSSSSSSSSS SSSSSSSSSS SSSSSSSSSS SS SSSSSSS SPSPAKPGPQ ALPKPASPKK PPPGERRSRS PRKPIDSLRD SRSLSYSPVE RRRPSPQPSP RDQQSSSSER GSR RGQRGD SRSPSHKRRR ETPSPRPMRH RSSRSP

UniProtKB: Serine/arginine repetitive matrix protein 2

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Macromolecule #17: Pre-mRNA-splicing factor CWC22 homolog

MacromoleculeName: Pre-mRNA-splicing factor CWC22 homolog / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 105.646578 KDa
SequenceString: MKSSVAQIKP SSGHDRRENL NSYQRNSSPE DRYEEQERSP RDRDYFDYSR SDYEHSRRGR SYDSSMESRN RDREKRRERE RDTDRKRSR KSPSPGRRNP ETSVTQSSSA QDEPATKKKK DELDPLLTRT GGAYIPPAKL RMMQEQITDK NSLAYQRMSW E ALKKSING ...String:
MKSSVAQIKP SSGHDRRENL NSYQRNSSPE DRYEEQERSP RDRDYFDYSR SDYEHSRRGR SYDSSMESRN RDREKRRERE RDTDRKRSR KSPSPGRRNP ETSVTQSSSA QDEPATKKKK DELDPLLTRT GGAYIPPAKL RMMQEQITDK NSLAYQRMSW E ALKKSING LINKVNISNI SIIIQELLQE NIVRGRGLLS RSVLQAQSAS PIFTHVYAAL VAIINSKFPQ IGELILKRLI LN FRKGYRR NDKQLCLTAS KFVAHLINQN VAHEVLCLEM LTLLLERPTD DSVEVAIGFL KECGLKLTQV SPRGINAIFE RLR NILHES EIDKRVQYMI EVMFAVRKDG FKDHPIILEG LDLVEEDDQF THMLPLEDDY NPEDVLNVFK MDPNFMENEE KYKA IKKEI LDEGDTDSNT DQDAGSSEED EEEEEEEGEE DEEGQKVTIH DKTEINLVSF RRTIYLAIQS SLDFEECAHK LLKME FPES QTKELCNMIL DCCAQQRTYE KFFGLLAGRF CMLKKEYMES FEGIFKEQYD TIHRLETNKL RNVAKMFAHL LYTDSL PWS VLECIKLSEE TTTSSSRIFV KIFFQELCEY MGLPKLNARL KDETLQPFFE GLLPRDNPRN TRFAINFFTS IGLGGLT DE LREHLKNTPK VIVAQKPDVE QNKSSPSSSS SASSSSESDS SDSDSDSSDS SSESSSEESD SSSISSHSSA SANDVRKK G HGKTRSKEVD KLIRNQQTND RKQKERRQEH GHQETRTERE RRSEKHRDQN SSGSNWRDPI TKYTSDKDVP SERNNYSRV ANDRDQEMHI DLENKHGDPK KKRGERRNSF SENEKHTHRI KDSENFRRKD RSKSKEMNRK HSGSRSDEDR YQNGAERRWE KSSRYSEQS RESKKNQDRR REKSPAKQK

UniProtKB: Pre-mRNA-splicing factor CWC22 homolog

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Macromolecule #18: Peptidyl-prolyl cis-trans isomerase-like 1

MacromoleculeName: Peptidyl-prolyl cis-trans isomerase-like 1 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO / EC number: peptidylprolyl isomerase
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 18.257805 KDa
SequenceString:
MAAIPPDSWQ PPNVYLETSM GIIVLELYWK HAPKTCKNFA ELARRGYYNG TKFHRIIKDF MIQGGDPTGT GRGGASIYGK QFEDELHPD LKFTGAGILA MANAGPDTNG SQFFVTLAPT QWLDGKHTIF GRVCQGIGMV NRVGMVETNS QDRPVDDVKI I KAYPSG

UniProtKB: Peptidyl-prolyl cis-trans isomerase-like 1

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Macromolecule #20: WD repeat-containing protein 70

MacromoleculeName: WD repeat-containing protein 70 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 73.306172 KDa
SequenceString: MERSGPSEVT GSDASGPDPQ LAVTMGFTGF GKKARTFDLE AMFEQTRRTA VERSRKTLEA REKEEEMNRE KELRRQNEDI EPTSSRSNV VRDCSKSSSR DTSSSESEQS SDSSDDELIG PPLPPKMVGK PVNFMEEDIL GPLPPPLNEE EEEAEEEEEE E EEEENPVH ...String:
MERSGPSEVT GSDASGPDPQ LAVTMGFTGF GKKARTFDLE AMFEQTRRTA VERSRKTLEA REKEEEMNRE KELRRQNEDI EPTSSRSNV VRDCSKSSSR DTSSSESEQS SDSSDDELIG PPLPPKMVGK PVNFMEEDIL GPLPPPLNEE EEEAEEEEEE E EEEENPVH KIPDSHEITL KHGTKTVSAL GLDPSGARLV TGGYDYDVKF WDFAGMDASF KAFRSLQPCE CHQIKSLQYS NT GDMILVV SGSSQAKVID RDGFEVMECI KGDQYIVDMA NTKGHTAMLH TGSWHPKIKG EFMTCSNDAT VRTWEVENPK KQK SVFKPR TMQGKKVIPT TCTYSRDGNL IAAACQNGSI QIWDRNLTVH PKFHYKQAHD SGTDTSCVTF SYDGNVLASR GGDD SLKLW DIRQFNKPLF SASGLPTMFP MTDCCFSPDD KLIVTGTSIQ RGCGSGKLVF FERRTFQRVY EIDITDASVV RCLWH PKLN QIMVGTGNGL AKVYYDPNKS QRGAKLCVVK TQRKAKQAET LTQDYIITPH ALPMFREPRQ RSTRKQLEKD RLDPLK SHK PEPPVAGPGR GGRVGTHGGT LSSYIVKNIA LDKTDDSNPR EAILRHAKAA EDSPYWVSPA YSKTQPKTMF AQVESDD EE AKNEPEWKKR KI

UniProtKB: WD repeat-containing protein 70

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Macromolecule #21: Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16

MacromoleculeName: Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16
type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA helicase
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 140.720641 KDa
SequenceString: MGDTSEDASI HRLEGTDLDC QVGGLICKSK SAASEQHVFK APAPRPSLLG LDLLASLKRR EREEKDDGED KKKSKVSSYK DWEESKDDQ KDAEEEGGDQ AGQNIRKDRH YRSARVETPS HPGGVSEEFW ERSRQRERER REHGVYASSK EEKDWKKEKS R DRDYDRKR ...String:
MGDTSEDASI HRLEGTDLDC QVGGLICKSK SAASEQHVFK APAPRPSLLG LDLLASLKRR EREEKDDGED KKKSKVSSYK DWEESKDDQ KDAEEEGGDQ AGQNIRKDRH YRSARVETPS HPGGVSEEFW ERSRQRERER REHGVYASSK EEKDWKKEKS R DRDYDRKR DRDERDRSRH SSRSERDGGS ERSSRRNEPE SPRHRPKDAA TPSRSTWEEE DSGYGSSRRS QWESPSPTPS YR DSERSHR LSTRDRDRSV RGKYSDDTPL PTPSYKYNEW ADDRRHLGST PRLSRGRGRR EEGEEGISFD TEEERQQWED DQR QADRDW YMMDEGYDEF HNPLAYSSED YVRRREQHLH KQKQKRISAQ RRQINEDNER WETNRMLTSG VVHRLEVDED FEED NAAKV HLMVHNLVPP FLDGRIVFTK QPEPVIPVKD ATSDLAIIAR KGSQTVRKHR EQKERKKAQH KHWELAGTKL GDIMG VKKE EEPDKAVTED GKVDYRTEQK FADHMKRKSE ASSEFAKKKS ILEQRQYLPI FAVQQELLTI IRDNSIVIVV GETGSG KTT QLTQYLHEDG YTDYGMIGCT QPRRVAAMSV AKRVSEEMGG NLGEEVGYAI RFEDCTSENT LIKYMTDGIL LRESLRE AD LDHYSAIIMD EAHERSLNTD VLFGLLREVV ARRSDLKLIV TSATMDAEKF AAFFGNVPIF HIPGRTFPVD ILFSKTPQ E DYVEAAVKQS LQVHLSGAPG DILIFMPGQE DIEVTSDQIV EHLEELENAP ALAVLPIYSQ LPSDLQAKIF QKAPDGVRK CIVATNIAET SLTVDGIMFV IDSGYCKLKV FNPRIGMDAL QIYPISQANA NQRSGRAGRT GPGQCFRLYT QSAYKNELLT TTVPEIQRT NLANVVLLLK SLGVQDLLQF HFMDPPPEDN MLNSMYQLWI LGALDNTGGL TSTGRLMVEF PLDPALSKML I VSCDMGCS SEILLIVSML SVPAIFYRPK GREEESDQIR EKFAVPESDH LTYLNVYLQW KNNNYSTIWC NDHFIHAKAM RK VREVRAQ LKDIMVQQRM SLASCGTDWD IVRKCICAAY FHQAAKLKGI GEYVNIRTGM PCHLHPTSSL FGMGYTPDYI VYH ELVMTT KEYMQCVTAV DGEWLAELGP MFYSVKQAGK SRQENRRRAK EEASAMEEEM ALAEEQLRAR RQEQEKRSPL GSVR STKIY TPGRKEQGEP MTPRRTPARF GL

UniProtKB: Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16

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Macromolecule #22: Pre-mRNA-splicing factor ISY1 homolog

MacromoleculeName: Pre-mRNA-splicing factor ISY1 homolog / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 33.046254 KDa
SequenceString: MARNAEKAMT ALARFRQAQL EEGKVKERRP FLASECTELP KAEKWRRQII GEISKKVAQI QNAGLGEFRI RDLNDEINKL LREKGHWEV RIKELGGPDY GKVGPKMLDH EGKEVPGNRG YKYFGAAKDL PGVRELFEKE PLPPPRKTRA ELMKAIDFEY Y GYLDEDDG ...String:
MARNAEKAMT ALARFRQAQL EEGKVKERRP FLASECTELP KAEKWRRQII GEISKKVAQI QNAGLGEFRI RDLNDEINKL LREKGHWEV RIKELGGPDY GKVGPKMLDH EGKEVPGNRG YKYFGAAKDL PGVRELFEKE PLPPPRKTRA ELMKAIDFEY Y GYLDEDDG VIVPLEQEYE KKLRAELVEK WKAEREARLA RGEKEEEEEE EEEINIYAVT EEESDEEGSQ EKGGDDSQQK FI AHVPVPS QQEIEEALVR RKKMELLQKY ASETLQAQSE EARRLLGY

UniProtKB: Pre-mRNA-splicing factor ISY1 homolog

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Macromolecule #23: Pre-mRNA-splicing factor CWC25 homolog

MacromoleculeName: Pre-mRNA-splicing factor CWC25 homolog / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 49.769938 KDa
SequenceString: MGGGDLNLKK SWHPQTLRNV EKVWKAEQKH EAERKKIEEL QRELREERAR EEMQRYAEDV GAVKKKEEKL DWMYQGPGGM VNRDEYLLG RPIDKYVFEK MEEKEAGCSS ETGLLPGSIF APSGANSLLD MASKIREDPL FIIRKKEEEK KREVLNNPVK M KKIKELLQ ...String:
MGGGDLNLKK SWHPQTLRNV EKVWKAEQKH EAERKKIEEL QRELREERAR EEMQRYAEDV GAVKKKEEKL DWMYQGPGGM VNRDEYLLG RPIDKYVFEK MEEKEAGCSS ETGLLPGSIF APSGANSLLD MASKIREDPL FIIRKKEEEK KREVLNNPVK M KKIKELLQ MSLEKKEKKK KKEKKKKHKK HKHRSSSSDR SSSEDEHSAG RSQKKMANSS PVLSKVPGYG LQVRNSDRNQ GL QGPLTAE QKRGHGMKNH SRSRSSSHSP PRHASKKSTR EAGSRDRRSR SLGRRSRSPR PSKLHNSKVN RRETGQTRSP SPK KEVYQR RHAPGYTRKL SAEELERKRQ EMMENAKWRE EERLNILKRH AKDEEREQRL EKLDSRDGKF IHRMKLESAS TSSL EDRVK RNIYSLQRTS VALEKNFMKR

UniProtKB: Pre-mRNA-splicing factor CWC25 homolog

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Macromolecule #24: Splicing factor YJU2

MacromoleculeName: Splicing factor YJU2 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 21.452719 KDa
SequenceString:
MSERKVLNKY YPPDFDPSKI PKLKLPKDRQ YVVRLMAPFN MRCKTCGEYI YKGKKFNARK ETVQNEVYLG LPIFRFYIKC TRCLAEITF KTDPENTDYT MEHGATRNFQ AEKLLEEEEK RVQKEREDEE LNNPMKVLEN RTKDSKLEME VLENLQELKD L NQRQAHVD FEAMLRQHRL

UniProtKB: Splicing factor YJU2

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Macromolecule #25: Protein FRG1

MacromoleculeName: Protein FRG1 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 29.227492 KDa
SequenceString: MAEYSYVKST KLVLKGTKTK SKKKKSKDKK RKREEDEETQ LDIVGIWWTV TNFGEISGTI AIEMDKGTYI HALDNGLFTL GAPHKEVDE GPSPPEQFTA VKLSDSRIAL KSGYGKYLGI NSDGLVVGRS DAIGPREQWE PVFQNGKMAL LASNSCFIRC N EAGDIEAK ...String:
MAEYSYVKST KLVLKGTKTK SKKKKSKDKK RKREEDEETQ LDIVGIWWTV TNFGEISGTI AIEMDKGTYI HALDNGLFTL GAPHKEVDE GPSPPEQFTA VKLSDSRIAL KSGYGKYLGI NSDGLVVGRS DAIGPREQWE PVFQNGKMAL LASNSCFIRC N EAGDIEAK SKTAGEEEMI KIRSCAERET KKKDDIPEED KGNVKQCEIN YVKKFQSFQD HKLKISKEDS KILKKARKDG FL HETLLDR RAKLKADRYC K

UniProtKB: Protein FRG1

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Macromolecule #26: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 26 / Number of copies: 5 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #27: INOSITOL HEXAKISPHOSPHATE

MacromoleculeName: INOSITOL HEXAKISPHOSPHATE / type: ligand / ID: 27 / Number of copies: 1 / Formula: IHP
Molecular weightTheoretical: 660.035 Da
Chemical component information

ChemComp-IHP:
INOSITOL HEXAKISPHOSPHATE

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Macromolecule #28: GUANOSINE-5'-TRIPHOSPHATE

MacromoleculeName: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 28 / Number of copies: 1 / Formula: GTP
Molecular weightTheoretical: 523.18 Da
Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

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Macromolecule #29: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 29 / Number of copies: 7 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.1 mg/mL
BufferpH: 7.9
Component:
ConcentrationFormulaName
20.0 mMHEPES-KOHHEPES-KOH, pH 7.9
1.5 mMMgCl2Magnesium Chloride
150.0 mMNaClSodium chloride
GridModel: Quantifoil / Material: COPPER / Mesh: 200 / Support film - Material: CARBON / Support film - topology: HOLEY ARRAY
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK II
DetailsMonodisperse particle distribution

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: FEI FALCON III (4k x 4k) / Detector mode: INTEGRATING / Digitization - Dimensions - Width: 4096 pixel / Digitization - Dimensions - Height: 4096 pixel / Number grids imaged: 2 / Number real images: 28279 / Average exposure time: 1.0 sec. / Average electron dose: 120.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 70.0 µm / Illumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: -0.003 µm / Nominal defocus min: -0.001 µm / Nominal magnification: 132000
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 1751359
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: OTHER
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.4 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 411539
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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