|Entry||Database: EMDB / ID: EMD-11381|
|Title||Treponema denticola chemotaxis signalling arrays in an ODP (TDE2498) deletion mutant|
|Sample||Chemotaxis arrays in a Treponema denticola ODP (TDE2498) deletion mutant|
|Biological species||Treponema denticola (bacteria)|
|Method||subtomogram averaging / cryo EM / Resolution: 28.2 Å|
|Authors||Muok AR / Yang W / Briegel A|
|Funding support|| Netherlands, 1 items |
|Citation||Journal: Nat Commun / Year: 2020|
Title: Atypical chemoreceptor arrays accommodate high membrane curvature.
Authors: Alise R Muok / Davi R Ortega / Kurni Kurniyati / Wen Yang / Zachary A Maschmann / Adam Sidi Mabrouk / Chunhao Li / Brian R Crane / Ariane Briegel /
Abstract: The prokaryotic chemotaxis system is arguably the best-understood signaling pathway in biology. In all previously described species, chemoreceptors organize into a hexagonal (P6 symmetry) extended ...The prokaryotic chemotaxis system is arguably the best-understood signaling pathway in biology. In all previously described species, chemoreceptors organize into a hexagonal (P6 symmetry) extended array. Here, we report an alternative symmetry (P2) of the chemotaxis apparatus that emerges from a strict linear organization of the histidine kinase CheA in Treponema denticola cells, which possesses arrays with the highest native curvature investigated thus far. Using cryo-ET, we reveal that Td chemoreceptor arrays assume an unusual arrangement of the supra-molecular protein assembly that has likely evolved to accommodate the high membrane curvature. The arrays have several atypical features, such as an extended dimerization domain of CheA and a variant CheW-CheR-like fusion protein that is critical for maintaining an ordered chemosensory apparatus. Furthermore, the previously characterized Td oxygen sensor ODP influences CheA ordering. These results suggest a greater diversity of the chemotaxis signaling system than previously thought.
|Structure viewer||EM map: |
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|File||Download / File: emd_11381.map.gz / Format: CCP4 / Size: 8 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)|
|Projections & slices|
Images are generated by Spider.
|Voxel size||X=Y=Z: 7.026 Å|
|Symmetry||Space group: 1|
CCP4 map header:
-Entire Chemotaxis arrays in a Treponema denticola ODP (TDE2498) deletion...
|Entire||Name: Chemotaxis arrays in a Treponema denticola ODP (TDE2498) deletion mutant|
Number of Components: 1
-Component #1: cellular-component, Chemotaxis arrays in a Treponema denticola OD...
|Cellular-component||Name: Chemotaxis arrays in a Treponema denticola ODP (TDE2498) deletion mutant|
Recombinant expression: No
|Source||Species: Treponema denticola (bacteria)|
|Specimen||Specimen State: Cell / Method: cryo EM|
|Sample solution||pH: 7.5|
|Vitrification||Instrument: LEICA PLUNGER / Cryogen Name: ETHANE / Temperature: 20 K / Humidity: 95 %|
-Electron microscopy imaging
Model: Titan Krios / Image courtesy: FEI Company
|Imaging||Microscope: FEI TITAN KRIOS|
|Electron gun||Electron Source: FIELD EMISSION GUN / Accelerating Voltage: 300 kV / Electron Dose: 1.6 e/Å2 / Illumination Mode: FLOOD BEAM|
|Lens||Imaging Mode: BRIGHT FIELD|
|Specimen Holder||Model: OTHER|
|Camera||Detector: GATAN K2 SUMMIT (4k x 4k)|
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