PDB-7a5k: Structure of the human mitoribosome in the post translocation sta...

Basic information

• Entry: Database: PDB / ID: 7a5k
• Title: Structure of the human mitoribosome in the post translocation state bound to mtEF-G1

Components

• (28S ribosomal protein ...) x 27
• (39S ribosomal protein ...) x 46
• (Mitochondrial ...) x 2
• 12S rRNA
• 16S rRNA
• Aurora kinase A-interacting protein
• Coiled-coil-helix-coiled-coil-helix domain-containing protein 1
• Elongation factor G, mitochondrial
• Growth arrest and DNA damage-inducible proteins-interacting protein 1
• Pentatricopeptide repeat domain-containing protein 3, mitochondrial
• Peptidyl-tRNA hydrolase ICT1, mitochondrial
• RNA
• Ribosomal protein 63, mitochondrial
• Unknown protein
• cDNA FLJ76418, highly similar to Homo sapiens mitochondrial ribosomal protein L52 (MRPL52), transcript variant 1, mRNA
• mt-tRNA
• mt-tRNAVal
• nascent chain

• Keywords: RIBOSOME / mitochondrial ribosome / ribosome stalling / cryo-EM

Function / homology

Function:

mitochondrial protein quality control / protein insertion into mitochondrial inner membrane from matrix / mitochondrial ribosome binding / membrane insertase activity / negative regulation of oxidoreductase activity / Complex I biogenesis / mitochondrial translational termination / mitochondrial translational elongation / mitochondrial ribosome assembly / translation release factor activity, codon nonspecific / negative regulation of ATP-dependent activity / Mitochondrial translation elongation / Mitochondrial translation termination / Mitochondrial translation initiation / mitochondrial proton-transporting ATP synthase complex assembly / translation release factor activity / mitochondrial large ribosomal subunit / negative regulation of mitotic nuclear division / peptidyl-tRNA hydrolase / mitochondrial ribosome / Hydrolases; Acting on ester bonds; Endoribonucleases producing 5'-phosphomonoesters / mitochondrial small ribosomal subunit / peptidyl-tRNA hydrolase activity / mitochondrial translation / positive regulation of proteolysis / mitochondrial respiratory chain complex I assembly / ribosomal small subunit binding / anatomical structure morphogenesis / translation elongation factor activity / RNA processing / rescue of stalled ribosome / Mitochondrial protein degradation / cellular response to leukemia inhibitory factor / aerobic respiration / protein tetramerization / apoptotic signaling pathway / mitochondrial membrane / fibrillar center / cell junction / regulation of translation / large ribosomal subunit / double-stranded RNA binding / ribosomal small subunit assembly / small ribosomal subunit / large ribosomal subunit rRNA binding / small ribosomal subunit rRNA binding / endonuclease activity / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / nuclear membrane / tRNA binding / cell population proliferation / mitochondrial inner membrane / negative regulation of translation / rRNA binding / nuclear body / ribosome / structural constituent of ribosome / mitochondrial matrix / protein domain specific binding / translation / ribonucleoprotein complex / nucleotide binding / GTPase activity / intracellular membrane-bounded organelle / mRNA binding / apoptotic process / GTP binding / nucleolus / protein homodimerization activity / protein-containing complex / mitochondrion / RNA binding / nucleoplasm / nucleus / plasma membrane / cytosol / cytoplasm

Domain/homology:

Membrane insertase YidC/Oxa1, C-terminal / 60Kd inner membrane protein / Membrane insertase YidC/ALB3/OXA1/COX18 / Ribosomal protein L1, mitochondrial / PPR repeat family / Coiled-coil-helix-coiled-coil-helix domain-containing protein 1 / Cysteine alpha-hairpin motif superfamily / 28S ribosomal protein S26 / Mitochondrial ribosome subunit S26 / Translation elongation factor EFG/EF2 / 28S ribosomal protein S27, mitochondrial / MRPS27/PTCD2 / : / Mitochondrial 28S ribosomal protein S27 / Ribosomal protein S22, mitochondrial / Ribosomal protein S23, mitochondrial / Ribosomal protein S23/S25, mitochondrial / Mitochondrial 28S ribosomal protein S31 / : / Mitochondrial 28S ribosomal protein S22 / Mitochondrial ribosomal protein S23 / Mitochondrial 28S ribosomal protein S31 / Ribosomal protein S29, mitochondrial / Ribosomal protein S28, mitochondrial / Mitochondrial 28S ribosomal protein S34 / 28S ribosomal protein S10, mitochondrial / Mitochondrial ribosomal protein MRP-S35 / Mitochondrial 28S ribosomal protein S34 / 28S ribosomal protein S24, mitochondrial / Pentatricopeptide repeat domain-containing protein 3 / 28S ribosomal protein S17, mitochondrial / 28S ribosomal protein S18b, mitochondrial / : / : / Mitochondrial ribosome subunit S24 / Small ribosomal subunit protein uS5m, N-terminal / Small ribosomal subunit protein mS39, PPR / : / Elongation factor G, domain III / EFG, domain V / 28S ribosomal protein S25, mitochondrial / : / Ribosomal protein L55, mitochondrial / Mitochondrial ribosomal protein L55 superfamily / Mitochondrial ribosomal protein L55 / Mitochondrial ribosomal protein L48 / 39S ribosomal protein L40, mitochondrial / Ribosomal protein S30, mitochondrial / Ribosomal protein L53, mitochondrial / 39S ribosomal protein L53/MRP-L53 / 39S ribosomal protein L42, mitochondrial / Mitochondrial 28S ribosomal protein S32 / Ribosomal protein L37, mitochondrial / Ribosomal protein L28/L40, mitochondrial / Mitochondrial ribosomal protein L37 / Mitochondrial ribosomal protein L28 / Pentatricopeptide repeat domain / Ribosomal protein 63, mitochondrial / Growth arrest/ DNA-damage-inducible protein-interacting protein 1 / Ribosomal protein L51, mitochondrial / Growth arrest and DNA-damage-inducible proteins-interacting protein 1 / Mitochondrial ribosomal subunit / Mitochondrial ribosome protein 63 / Ribosomal protein L37/S30 / Growth arrest and DNA damage-inducible proteins-interacting protein 1 domain superfamily / : / : / Mitochondrial 28S ribosomal protein S30 (PDCD9) / Ribosomal protein S27/S33, mitochondrial / Ribosomal protein L35, mitochondrial / 39S ribosomal protein L52, mitochondrial / Ribosomal protein S24/S35, mitochondrial / Mitochondrial ribosomal subunit S27 / Mitoribosomal protein mL52 / : / MRPL44, double-stranded RNA binding domain / : / MRPL44 dsRNA-binding domain / Large ribosomal subunit protein mL44, endonuclease domain / : / Large ribosomal subunit protein bL9m C-terminal domain / Tim44-like domain / Tim44-like domain / Tim44 / Ribosomal protein S24/S35, mitochondrial, conserved domain / Mitochondrial ribosomal subunit protein / Mitochondrial ribosomal protein L46 NUDIX / : / : / Ribosomal protein L46, N-terminal / 39S mitochondrial ribosomal protein L46 / Pentatricopeptide (PPR) repeat profile. / Ribosomal protein L50, mitochondria / Ribosomal subunit 39S / Ribosomal protein L49/IMG2 / Mitochondrial large subunit ribosomal protein (Img2) / Ribosomal protein L27/L41, mitochondrial / : / Mitochondrial ribosomal protein L27 / Ribosomal protein S23/S29, mitochondrial

Component:

PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / GUANOSINE-5'-DIPHOSPHATE / SPERMIDINE / : / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Large ribosomal subunit protein mL52 / Mitochondrial ribosomal protein L18, isoform CRA_b / Large ribosomal subunit protein uL22m / Small ribosomal subunit protein uS12m / Small ribosomal subunit protein uS14m / Large ribosomal subunit protein bL33m / Large ribosomal subunit protein uL3m / Large ribosomal subunit protein bL19m / Small ribosomal subunit protein mS29 / Small ribosomal subunit protein mS22 / Small ribosomal subunit protein mS25 / Small ribosomal subunit protein uS10m / Small ribosomal subunit protein mS35 / Small ribosomal subunit protein uS5m / Small ribosomal subunit protein uS11m / Small ribosomal subunit protein uS15m / Small ribosomal subunit protein bS21m / Small ribosomal subunit protein mS34 / Small ribosomal subunit protein bS6m / Small ribosomal subunit protein uS9m / Large ribosomal subunit protein bL28m / Large ribosomal subunit protein mL49 / Large ribosomal subunit protein mL62 / Mitochondrial inner membrane protein OXA1L / Large ribosomal subunit protein uL23m / Large ribosomal subunit protein mL51 / Large ribosomal subunit protein uL2m / Large ribosomal subunit protein mL54 / Large ribosomal subunit protein uL14m / Large ribosomal subunit protein bL21m / Large ribosomal subunit protein mL55 / Large ribosomal subunit protein uL10m / Large ribosomal subunit protein mL41 / Large ribosomal subunit protein mL50 / Large ribosomal subunit protein mL43 / Large ribosomal subunit protein mL64 / Large ribosomal subunit protein uL30m / Small ribosomal subunit protein mS27 / Small ribosomal subunit protein mS31 / Large ribosomal subunit protein uL24m / Small ribosomal subunit protein mS37 / Large ribosomal subunit protein mL38 / Small ribosomal subunit protein uS3m / Large ribosomal subunit protein mL53 / Small ribosomal subunit protein mS39 / Large ribosomal subunit protein mL48 / Elongation factor G, mitochondrial / Large ribosomal subunit protein bL34m / Large ribosomal subunit protein mL63 / Large ribosomal subunit protein mL45 / Large ribosomal subunit protein bL32m / Large ribosomal subunit protein bL20m / Large ribosomal subunit protein uL13m / Large ribosomal subunit protein bL9m / Large ribosomal subunit protein uL4m / Large ribosomal subunit protein uL1m / Small ribosomal subunit protein mS26 / Large ribosomal subunit protein mL37 / Large ribosomal subunit protein mL46 / Large ribosomal subunit protein mL44 / Large ribosomal subunit protein uL29m / Large ribosomal subunit protein mL65 / Large ribosomal subunit protein mL40 / Large ribosomal subunit protein bL17m / Large ribosomal subunit protein mL66 / Small ribosomal subunit protein mS38 / Large ribosomal subunit protein uL16m / Large ribosomal subunit protein mL39 / Large ribosomal subunit protein bL35m / Large ribosomal subunit protein uL15m / Large ribosomal subunit protein bL36m / Large ribosomal subunit protein bL27m / Small ribosomal subunit protein mS33 / Small ribosomal subunit protein bS1m / Small ribosomal subunit protein uS17m / Small ribosomal subunit protein uS7m / Small ribosomal subunit protein uS2m / Large ribosomal subunit protein uL11m / Small ribosomal subunit protein bS16m / Small ribosomal subunit protein bS18m / Small ribosomal subunit protein mS23 / Small ribosomal subunit protein mS40 / Large ribosomal subunit protein mL42

• Biological species: Homo sapiens (human)
• Method: ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.7 Å
• Authors: Desai, N. / Yang, H. / Chandrasekaran, V. / Kazi, R. / Minczuk, M. / Ramakrishnan, V.

Funding support

United Kingdom, 3items

Organization	Grant number	Country
Medical Research Council (MRC, United Kingdom)	MC_U105184332	United Kingdom
Wellcome Trust	WT096570	United Kingdom
Louis-Jeantet Foundation		United Kingdom

• Citation: Journal: Science / Year: 2020; Title: Elongational stalling activates mitoribosome-associated quality control.; Authors: Nirupa Desai / Hanting Yang / Viswanathan Chandrasekaran / Razina Kazi / Michal Minczuk / V Ramakrishnan / ; Abstract: The human mitochondrial ribosome (mitoribosome) and associated proteins regulate the synthesis of 13 essential subunits of the oxidative phosphorylation complexes. We report the discovery of a mitoribosome-associated quality control pathway that responds to interruptions during elongation, and we present structures at 3.1- to 3.3-angstrom resolution of mitoribosomal large subunits trapped during ribosome rescue. Release factor homolog C12orf65 (mtRF-R) and RNA binding protein C6orf203 (MTRES1) eject the nascent chain and peptidyl transfer RNA (tRNA), respectively, from stalled ribosomes. Recruitment of mitoribosome biogenesis factors to these quality control intermediates suggests additional roles for these factors during mitoribosome rescue. We also report related cryo-electron microscopy structures (3.7 to 4.4 angstrom resolution) of elongating mitoribosomes bound to tRNAs, nascent polypeptides, the guanosine triphosphatase elongation factors mtEF-Tu and mtEF-G1, and the Oxa1L translocase.

History

Deposition	Aug 21, 2020	Deposition site: PDBE / Processing site: PDBE
Revision 1.0	Dec 23, 2020	Provider: repository / Type: Initial release
Revision 2.0	Dec 7, 2022	Group: Advisory / Atomic model / Data collection / Database references / Derived calculations / Polymer sequence / Source and taxonomy / Structure summary Category: atom_site / database_2 / entity / entity_name_com / entity_poly / entity_poly_seq / entity_src_nat / pdbx_entity_nonpoly / pdbx_nonpoly_scheme / pdbx_poly_seq_scheme / pdbx_struct_assembly / pdbx_struct_assembly_gen / pdbx_struct_conn_angle / pdbx_unobs_or_zero_occ_atoms / pdbx_unobs_or_zero_occ_residues / pdbx_validate_close_contact / pdbx_validate_peptide_omega / pdbx_validate_rmsd_angle / pdbx_validate_torsion / struct_asym / struct_conf / struct_conn / struct_ref / struct_ref_seq Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _entity.formula_weight / _entity.pdbx_description / _entity.pdbx_number_of_molecules / _entity.src_method / _entity.type / _pdbx_struct_assembly.oligomeric_count / _pdbx_struct_assembly.oligomeric_details / _pdbx_struct_assembly_gen.asym_id_list / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr2_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_validate_peptide_omega.auth_comp_id_1 / _pdbx_validate_peptide_omega.auth_comp_id_2

Assembly

Deposited unit

r1: Elongation factor G, mitochondrial

Y2: nascent chain

A3: 16S rRNA

B3: mt-tRNAVal

D3: 39S ribosomal protein L2, mitochondrial

E3: 39S ribosomal protein L3, mitochondrial

F3: 39S ribosomal protein L4, mitochondrial

H3: 39S ribosomal protein L9, mitochondrial

I3: 39S ribosomal protein L10, mitochondrial

J3: 39S ribosomal protein L11, mitochondrial

K3: 39S ribosomal protein L13, mitochondrial

L3: 39S ribosomal protein L14, mitochondrial

M3: 39S ribosomal protein L15, mitochondrial

N3: 39S ribosomal protein L16, mitochondrial

O3: 39S ribosomal protein L17, mitochondrial

P3: Mitochondrial ribosomal protein L18, isoform CRA_b

Q3: 39S ribosomal protein L19, mitochondrial

R3: 39S ribosomal protein L20, mitochondrial

S3: 39S ribosomal protein L21, mitochondrial

T3: 39S ribosomal protein L22, mitochondrial

U3: 39S ribosomal protein L23, mitochondrial

V3: 39S ribosomal protein L24, mitochondrial

W3: 39S ribosomal protein L27, mitochondrial

X3: 39S ribosomal protein L28, mitochondrial

Y3: 39S ribosomal protein L47, mitochondrial

Z3: 39S ribosomal protein L30, mitochondrial

03: 39S ribosomal protein L32, mitochondrial

13: 39S ribosomal protein L33, mitochondrial

23: 39S ribosomal protein L34, mitochondrial

33: 39S ribosomal protein L35, mitochondrial

43: 39S ribosomal protein L36, mitochondrial

53: 39S ribosomal protein L37, mitochondrial

63: 39S ribosomal protein L38, mitochondrial

73: 39S ribosomal protein L39, mitochondrial

93: 39S ribosomal protein L41, mitochondrial

a3: 39S ribosomal protein L42, mitochondrial

b3: 39S ribosomal protein L43, mitochondrial

c3: 39S ribosomal protein L44, mitochondrial

d3: 39S ribosomal protein L45, mitochondrial

e3: 39S ribosomal protein L46, mitochondrial

f3: 39S ribosomal protein L48, mitochondrial

g3: 39S ribosomal protein L49, mitochondrial

h3: 39S ribosomal protein L50, mitochondrial

i3: 39S ribosomal protein L51, mitochondrial

j3: cDNA FLJ76418, highly similar to Homo sapiens mitochondrial ribosomal protein L52 (MRPL52), transcript variant 1, mRNA

k3: 39S ribosomal protein L53, mitochondrial

l3: 39S ribosomal protein L54, mitochondrial

m3: 39S ribosomal protein L55, mitochondrial

o3: Ribosomal protein 63, mitochondrial

p3: Peptidyl-tRNA hydrolase ICT1, mitochondrial

q3: Growth arrest and DNA damage-inducible proteins-interacting protein 1

r3: 39S ribosomal protein S18a, mitochondrial

s3: 39S ribosomal protein S30, mitochondrial

u3: RNA

A5: Mitochondrial inner membrane protein OXA1L

B6: 28S ribosomal protein S2, mitochondrial

C6: 28S ribosomal protein S24, mitochondrial

D6: 28S ribosomal protein S5, mitochondrial

E6: 28S ribosomal protein S6, mitochondrial

F6: 28S ribosomal protein S7, mitochondrial

G6: 28S ribosomal protein S9, mitochondrial

H6: 28S ribosomal protein S10, mitochondrial

I6: 28S ribosomal protein S11, mitochondrial

J6: 28S ribosomal protein S12, mitochondrial

K6: 28S ribosomal protein S14, mitochondrial

L6: 28S ribosomal protein S15, mitochondrial

M6: 28S ribosomal protein S16, mitochondrial

N6: 28S ribosomal protein S17, mitochondrial

O6: 28S ribosomal protein S18b, mitochondrial

P6: 28S ribosomal protein S18c, mitochondrial

Q6: 28S ribosomal protein S21, mitochondrial

R6: 28S ribosomal protein S22, mitochondrial

S6: 28S ribosomal protein S23, mitochondrial

T6: 28S ribosomal protein S25, mitochondrial

U6: 28S ribosomal protein S26, mitochondrial

V6: 28S ribosomal protein S27, mitochondrial

W6: 28S ribosomal protein S28, mitochondrial

X6: 28S ribosomal protein S29, mitochondrial

Y6: 28S ribosomal protein S31, mitochondrial

Z6: 28S ribosomal protein S33, mitochondrial

a6: 28S ribosomal protein S34, mitochondrial

b6: 28S ribosomal protein S35, mitochondrial

c6: Coiled-coil-helix-coiled-coil-helix domain-containing protein 1

d6: Aurora kinase A-interacting protein

e6: Pentatricopeptide repeat domain-containing protein 3, mitochondrial

A6: 12S rRNA

24: mt-tRNA

8: 16S rRNA

A: 39S ribosomal protein L40, mitochondrial

D: 39S ribosomal protein L9, mitochondrial

FE: mt-tRNA

n: 39S ribosomal protein L1, mitochondrial

B: Unknown protein

hetero molecules

Summary:

• 3.63 MDa, 93 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	3,627,410	231
Polymers	3,622,731	93
Non-polymers	4,679	138
Water	0	0

1

Summary:

• Idetical with deposited unit
• defined by author

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555		1

Noncrystallographic symmetry (NCS)

NCS domain:

ID	Ens-ID	Details
d_1	ens_1	chain "24"
d_2	ens_1	chain "C"

NCS domain segments:

Dom-ID	Component-ID	Ens-ID	Beg label comp-ID	End label comp-ID	Label asym-ID
d_1	1	ens_1	G	A	IC
d_2	1	ens_1	G	A	C

NCS oper: (Code: given
Matrix: (0.889641061155, 0.143494096409, -0.433529960444), (0.00216523401307, 0.948012435547, 0.318225916306), (0.456655333997, -0.284045535686, 0.843080090851)
Vector: 38.415342595, -10.4331299971, -28.4251356339)

Components

Protein , 8 types, 8 molecules r1 j3 o3 p3 q3 c6 d6 e6

#1: Protein

r1 Elongation factor G, mitochondrial / EF-Gmt / Elongation factor G 1 / mitochondrial / mEF-G 1 / Elongation factor G1 / hEFG1

Details:

Mass: 83578.461 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q96RP9

#45: Protein

j3 cDNA FLJ76418, highly similar to Homo sapiens mitochondrial ribosomal protein L52 (MRPL52), transcript variant 1, mRNA

Details:

Mass: 13696.684 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: A8K7J6

#49: Protein

o3 Ribosomal protein 63, mitochondrial / hMRP63 / Mitochondrial large ribosomal subunit protein mL63 / Mitochondrial ribosomal protein 63 / Mitochondrial ribosomal protein L57

Details:

Mass: 12292.333 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9BQC6

#50: Protein

p3 Peptidyl-tRNA hydrolase ICT1, mitochondrial / 39S ribosomal protein L58 / mitochondrial / MRP-L58 / Digestion substraction 1 / DS-1 / Immature colon carcinoma transcript 1 protein / Mitochondrial large ribosomal subunit protein mL62

Details:

Mass: 23674.203 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q14197, peptidyl-tRNA hydrolase

#51: Protein

q3 Growth arrest and DNA damage-inducible proteins-interacting protein 1 / 39S ribosomal protein L59 / mitochondrial / MRP-L59 / CKII beta-associating protein / CR6-interacting factor 1 / CRIF1 / Mitochondrial large ribosomal subunit protein mL64 / Papillomavirus L2-interacting nuclear protein 1 / PLINP-1 / p53-responsive gene 6 protein

Details:

Mass: 25426.895 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q8TAE8

#83: Protein

c6 Coiled-coil-helix-coiled-coil-helix domain-containing protein 1 / 28S ribosomal protein S37 / mitochondrial / MRP-S37 / Mitochondrial small ribosomal subunit protein mS37 / Nuclear protein C2360

Details:

Mass: 13498.819 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q96BP2

#84: Protein

d6 Aurora kinase A-interacting protein / AURKA-interacting protein / 28S ribosomal protein S38 / mitochondrial / MRP-S38 / Mitochondrial small ribosomal subunit protein mS38

Details:

Mass: 22395.326 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9NWT8

#85: Protein

e6 Pentatricopeptide repeat domain-containing protein 3, mitochondrial / 28S ribosomal protein S39 / mitochondrial / MRP-S39 / Mitochondrial small ribosomal subunit protein mS39 / Transformation-related gene 15 protein / TRG-15

Details:

Mass: 78648.547 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q96EY7

Protein/peptide , 2 types, 2 molecules Y2 B

#2: Protein/peptide

Y2 nascent chain

Details:

Mass: 2486.056 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human)

#90: Protein/peptide

B Unknown protein

Details:

Mass: 2400.951 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human)

RNA chain , 5 types, 7 molecules A3 8 B3 u3 A6 24 FE

#3: RNA chain

A3 8 16S rRNA

Details:

Mass: 500019.594 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: GenBank: 1025814679

#4: RNA chain

B3 mt-tRNAVal

Details:

Mass: 23266.883 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human)

#54: RNA chain

u3 RNA

Details:

Mass: 589.430 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human)

#86: RNA chain

A6 12S rRNA

Details:

Mass: 306135.156 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human)

#87: RNA chain

24 FE mt-tRNA

Details:

Mass: 23378.979 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human)

39S ribosomal protein ... , 46 types, 47 molecules D3 E3 F3 H3 D I3 J3 K3 L3 M3 N3 O3 Q3 R3 S3 T3 U3 V3 W3 X3 Y3 Z3 03 13 23 33 43 53 63 73 ...

#5: Protein

D3 39S ribosomal protein L2, mitochondrial / MRP-L2 / Mitochondrial large ribosomal subunit protein uL2m

Details:

Mass: 33355.941 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q5T653

#6: Protein

E3 39S ribosomal protein L3, mitochondrial / MRP-L3 / Mitochondrial large ribosomal subunit protein uL3m

Details:

Mass: 38689.562 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: P09001

#7: Protein

F3 39S ribosomal protein L4, mitochondrial / MRP-L4 / Mitochondrial large ribosomal subunit protein uL4m

Details:

Mass: 34970.195 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9BYD3

#8: Protein

H3 D 39S ribosomal protein L9, mitochondrial / MRP-L9 / Mitochondrial large ribosomal subunit protein bL9m

Details:

Mass: 30296.355 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9BYD2

#9: Protein

I3 39S ribosomal protein L10, mitochondrial / MRP-L10 / 39S ribosomal protein L8 / mitochondrial / MRP-L8 / Mitochondrial large ribosomal subunit protein uL10m

Details:

Mass: 29322.529 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q7Z7H8

#10: Protein

J3 39S ribosomal protein L11, mitochondrial / MRP-L11 / Mitochondrial large ribosomal subunit protein uL11m

Details:

Mass: 20716.227 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9Y3B7

#11: Protein

K3 39S ribosomal protein L13, mitochondrial / MRP-L13 / Mitochondrial large ribosomal subunit protein uL13m

Details:

Mass: 20722.988 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9BYD1

#12: Protein

L3 39S ribosomal protein L14, mitochondrial / MRP-L14 / 39S ribosomal protein L32 / mitochondrial / MRP-L32 / Mitochondrial large ribosomal subunit protein uL14m

Details:

Mass: 15973.702 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q6P1L8

#13: Protein

M3 39S ribosomal protein L15, mitochondrial / MRP-L15 / Mitochondrial large ribosomal subunit protein uL15m

Details:

Mass: 33473.820 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9P015

#14: Protein

N3 39S ribosomal protein L16, mitochondrial / MRP-L16 / Mitochondrial large ribosomal subunit protein uL16m

Details:

Mass: 28496.285 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9NX20

#15: Protein

O3 39S ribosomal protein L17, mitochondrial / MRP-L17 / LYST-interacting protein 2 / Mitochondrial large ribosomal subunit protein bL17m

Details:

Mass: 20083.266 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9NRX2

#17: Protein

Q3 39S ribosomal protein L19, mitochondrial / MRP-L19 / 39S ribosomal protein L15 / mitochondrial / MRP-L15 / Mitochondrial large ribosomal subunit protein bL19m

Details:

Mass: 33584.996 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: P49406

#18: Protein

R3 39S ribosomal protein L20, mitochondrial / MRP-L20 / Mitochondrial large ribosomal subunit protein bL20m

Details:

Mass: 17479.654 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9BYC9

#19: Protein

S3 39S ribosomal protein L21, mitochondrial / MRP-L21 / Mitochondrial large ribosomal subunit protein bL21m

Details:

Mass: 22847.682 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q7Z2W9

#20: Protein

T3 39S ribosomal protein L22, mitochondrial

Details:

Mass: 24369.527 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: E7ESL0

#21: Protein

U3 39S ribosomal protein L23, mitochondrial / MRP-L23 / L23 mitochondrial-related protein / Mitochondrial large ribosomal subunit protein uL23m / Ribosomal protein L23-like

Details:

Mass: 17807.189 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q16540

#22: Protein

V3 39S ribosomal protein L24, mitochondrial / MRP-L24 / Mitochondrial large ribosomal subunit protein uL24m

Details:

Mass: 24953.875 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q96A35

#23: Protein

W3 39S ribosomal protein L27, mitochondrial / MRP-L27 / Mitochondrial large ribosomal subunit protein bL27m

Details:

Mass: 16102.859 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9P0M9

#24: Protein

X3 39S ribosomal protein L28, mitochondrial / MRP-L28 / Melanoma antigen p15 / Melanoma-associated antigen recognized by T-lymphocytes / Mitochondrial large ribosomal subunit protein bL28m

Details:

Mass: 30090.676 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q13084

#25: Protein

Y3 39S ribosomal protein L47, mitochondrial / MRP-L47 / Mitochondrial large ribosomal subunit protein uL29m / Nasopharyngeal carcinoma metastasis-related protein 1

Details:

Mass: 29508.535 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9HD33

#26: Protein

Z3 39S ribosomal protein L30, mitochondrial / MRP-L30 / 39S ribosomal protein L28 / mitochondrial / MRP-L28 / Mitochondrial large ribosomal subunit protein uL30m

Details:

Mass: 18582.812 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q8TCC3

#27: Protein

03 39S ribosomal protein L32, mitochondrial / MRP-L32 / Mitochondrial large ribosomal subunit protein bL32m

Details:

Mass: 21437.799 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9BYC8

#28: Protein

13 39S ribosomal protein L33, mitochondrial / MRP-L33 / Mitochondrial large ribosomal subunit protein bL33m

Details:

Mass: 7635.204 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: O75394

#29: Protein

23 39S ribosomal protein L34, mitochondrial / MRP-L34 / Mitochondrial large ribosomal subunit protein bL34m

Details:

Mass: 10183.913 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9BQ48

#30: Protein

33 39S ribosomal protein L35, mitochondrial / MRP-L35 / Mitochondrial large ribosomal subunit protein bL35m

Details:

Mass: 21558.488 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9NZE8

#31: Protein

43 39S ribosomal protein L36, mitochondrial / MRP-L36 / BRCA1-interacting protein 1 / Mitochondrial large ribosomal subunit protein bL36m

Details:

Mass: 11809.301 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9P0J6

#32: Protein

53 39S ribosomal protein L37, mitochondrial / MRP-L37 / 39S ribosomal protein L2 / mitochondrial / MRP-L2 / Mitochondrial large ribosomal subunit protein mL37

Details:

Mass: 48183.344 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9BZE1

#33: Protein

63 39S ribosomal protein L38, mitochondrial / MRP-L38 / Mitochondrial large ribosomal subunit protein mL38

Details:

Mass: 44659.430 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q96DV4

#34: Protein

73 39S ribosomal protein L39, mitochondrial / MRP-L39 / 39S ribosomal protein L5 / mitochondrial / MRP-L5 / Mitochondrial large ribosomal subunit protein mL39

Details:

Mass: 38764.785 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9NYK5

#35: Protein

93 39S ribosomal protein L41, mitochondrial / MRP-L41 / 39S ribosomal protein L27 homolog / Bcl-2-interacting mitochondrial ribosomal protein L41 / Cell proliferation-inducing gene 3 protein / MRP-L27 homolog / Mitochondrial large ribosomal subunit protein mL41

Details:

Mass: 15404.708 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q8IXM3

#36: Protein

a3 39S ribosomal protein L42, mitochondrial / MRP-L42 / 39S ribosomal protein L31 / mitochondrial / MRP-L31 / Mitochondrial large ribosomal subunit protein mL42

Details:

Mass: 16691.176 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9Y6G3

#37: Protein

b3 39S ribosomal protein L43, mitochondrial / MRP-L43 / Mitochondrial large ribosomal subunit protein mL43 / Mitochondrial ribosomal protein bMRP36a

Details:

Mass: 17452.789 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q8N983

#38: Protein

c3 39S ribosomal protein L44, mitochondrial / MRP-L44 / Mitochondrial large ribosomal subunit protein mL44

Details:

Mass: 37583.312 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human)
References: UniProt: Q9H9J2, Hydrolases; Acting on ester bonds; Endoribonucleases producing 5'-phosphomonoesters

#39: Protein

d3 39S ribosomal protein L45, mitochondrial / MRP-L45 / Mitochondrial large ribosomal subunit protein mL45

Details:

Mass: 35402.918 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9BRJ2

#40: Protein

e3 39S ribosomal protein L46, mitochondrial / MRP-L46 / Mitochondrial large ribosomal subunit protein mL46 / P2ECSL

Details:

Mass: 31748.107 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9H2W6

#41: Protein

f3 39S ribosomal protein L48, mitochondrial / MRP-L48 / Mitochondrial large ribosomal subunit protein mL48

Details:

Mass: 21856.232 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q96GC5

#42: Protein

g3 39S ribosomal protein L49, mitochondrial / MRP-L49 / Mitochondrial large ribosomal subunit protein mL49 / Neighbor of FAU / NOF / Protein NOF1

Details:

Mass: 19225.043 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q13405

#43: Protein

h3 39S ribosomal protein L50, mitochondrial / MRP-L50 / Mitochondrial large ribosomal subunit protein mL50

Details:

Mass: 18349.943 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q8N5N7

#44: Protein

i3 39S ribosomal protein L51, mitochondrial / MRP-L51 / Mitochondrial large ribosomal subunit protein mL51 / bMRP-64 / bMRP64

Details:

Mass: 15125.917 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q4U2R6

#46: Protein

k3 39S ribosomal protein L53, mitochondrial / MRP-L53 / Mitochondrial large ribosomal subunit protein mL53

Details:

Mass: 12125.859 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q96EL3

#47: Protein

l3 39S ribosomal protein L54, mitochondrial / MRP-L54 / Mitochondrial large ribosomal subunit protein mL54

Details:

Mass: 15844.178 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q6P161

#48: Protein

m3 39S ribosomal protein L55, mitochondrial / MRP-L55 / Mitochondrial large ribosomal subunit protein bL31m / Mitochondrial large ribosomal subunit protein mL55

Details:

Mass: 15160.433 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q7Z7F7

#52: Protein

r3 39S ribosomal protein S18a, mitochondrial / S18mt-a / 39S ribosomal protein S18-3 / mitochondrial / MRP-S18-3 / Mitochondrial large ribosomal subunit protein bS18a / Mitochondrial large ribosomal subunit protein mL66

Details:

Mass: 22224.078 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9NVS2

#53: Protein

s3 39S ribosomal protein S30, mitochondrial / S30mt / Mitochondrial large ribosomal subunit protein mL65 / Mitochondrial large ribosomal subunit protein mS30 / Programmed cell death protein 9

Details:

Mass: 50429.578 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9NP92

#88: Protein

A 39S ribosomal protein L40, mitochondrial / MRP-L40 / Mitochondrial large ribosomal subunit protein mL40 / Nuclear localization signal-containing protein deleted in velocardiofacial syndrome / Up-regulated in metastasis

Details:

Mass: 24534.422 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9NQ50

#89: Protein

n 39S ribosomal protein L1, mitochondrial / MRP-L1 / Mitochondrial large ribosomal subunit protein uL1m

Details:

Mass: 32564.641 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9BYD6

Mitochondrial ... , 2 types, 2 molecules P3 A5

#16: Protein

P3 Mitochondrial ribosomal protein L18, isoform CRA_b / cDNA FLJ76688 / highly similar to Homo sapiens HSPC071 mRNA

Details:

Mass: 20465.348 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: A8K9D2

#55: Protein

A5 Mitochondrial inner membrane protein OXA1L / Hsa / OXA1Hs / Oxidase assembly 1-like protein / OXA1-like protein

Details:

Mass: 48602.688 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q15070

28S ribosomal protein ... , 27 types, 27 molecules B6 C6 D6 E6 F6 G6 H6 I6 J6 K6 L6 M6 N6 O6 P6 Q6 R6 S6 T6 U6 V6 W6 X6 Y6 Z6 a6 b6

#56: Protein

B6 28S ribosomal protein S2, mitochondrial / S2mt / Mitochondrial small ribosomal subunit protein uS2m

Details:

Mass: 33298.238 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9Y399

#57: Protein

C6 28S ribosomal protein S24, mitochondrial / S24mt / Mitochondrial small ribosomal subunit protein uS3m / bMRP-47 / bMRP47

Details:

Mass: 19046.164 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q96EL2

#58: Protein

D6 28S ribosomal protein S5, mitochondrial / S5mt / Mitochondrial small ribosomal subunit protein uS5m

Details:

Mass: 48094.594 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: P82675

#59: Protein

E6 28S ribosomal protein S6, mitochondrial / S6mt / Mitochondrial small ribosomal subunit protein bS6m

Details:

Mass: 14250.588 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: P82932

#60: Protein

F6 28S ribosomal protein S7, mitochondrial / S7mt / Mitochondrial small ribosomal subunit protein uS7m / bMRP-27a / bMRP27a

Details:

Mass: 28186.154 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9Y2R9

#61: Protein

G6 28S ribosomal protein S9, mitochondrial / S9mt / Mitochondrial small ribosomal subunit protein uS9m

Details:

Mass: 45909.859 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: P82933

#62: Protein

H6 28S ribosomal protein S10, mitochondrial / S10mt / Mitochondrial small ribosomal subunit protein uS10m

Details:

Mass: 23033.500 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: P82664

#63: Protein

I6 28S ribosomal protein S11, mitochondrial / S11mt / Cervical cancer proto-oncogene 2 protein / HCC-2 / Mitochondrial small ribosomal subunit protein uS11m

Details:

Mass: 20648.793 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: P82912

#64: Protein

J6 28S ribosomal protein S12, mitochondrial / S12mt / MT-RPS12 / Mitochondrial small ribosomal subunit protein uS12m

Details:

Mass: 15200.947 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: O15235

#65: Protein

K6 28S ribosomal protein S14, mitochondrial / S14mt / Mitochondrial small ribosomal subunit protein uS14m

Details:

Mass: 15168.788 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: O60783

#66: Protein

L6 28S ribosomal protein S15, mitochondrial / S15mt / Mitochondrial small ribosomal subunit protein uS15m

Details:

Mass: 29903.293 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: P82914

#67: Protein

M6 28S ribosomal protein S16, mitochondrial / S16mt / Mitochondrial small ribosomal subunit protein bS16m

Details:

Mass: 15371.899 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9Y3D3

#68: Protein

N6 28S ribosomal protein S17, mitochondrial / S17mt / Mitochondrial small ribosomal subunit protein uS17m

Details:

Mass: 14526.078 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9Y2R5

#69: Protein

O6 28S ribosomal protein S18b, mitochondrial / S18mt-b / 28S ribosomal protein S18-2 / mitochondrial / MRP-S18-2 / Mitochondrial small ribosomal subunit protein bS18b / Mitochondrial small ribosomal subunit protein mS40

Details:

Mass: 29440.629 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9Y676

#70: Protein

P6 28S ribosomal protein S18c, mitochondrial / S18mt-c / 28S ribosomal protein S18-1 / mitochondrial / MRP-S18-1 / Mitochondrial small ribosomal subunit protein bS18c / Mitochondrial small ribosomal subunit protein bS18m

Details:

Mass: 15876.786 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9Y3D5

#71: Protein

Q6 28S ribosomal protein S21, mitochondrial / S21mt / Mitochondrial small ribosomal subunit protein bS21m

Details:

Mass: 10764.652 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: P82921

#72: Protein

R6 28S ribosomal protein S22, mitochondrial / S22mt / Mitochondrial small ribosomal subunit protein mS22

Details:

Mass: 41337.316 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: P82650

#73: Protein

S6 28S ribosomal protein S23, mitochondrial / S23mt / Mitochondrial small ribosomal subunit protein mS23

Details:

Mass: 21805.664 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9Y3D9

#74: Protein

T6 28S ribosomal protein S25, mitochondrial / S25mt / Mitochondrial small ribosomal subunit protein mS25

Details:

Mass: 20146.428 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: P82663

#75: Protein

U6 28S ribosomal protein S26, mitochondrial / S26mt / 28S ribosomal protein S13 / mitochondrial / S13mt / Mitochondrial small ribosomal subunit protein mS26

Details:

Mass: 24259.820 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9BYN8

#76: Protein

V6 28S ribosomal protein S27, mitochondrial / S27mt / Mitochondrial ribosomal protein S27 / Mitochondrial small ribosomal subunit protein mS27

Details:

Mass: 47669.262 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q92552

#77: Protein

W6 28S ribosomal protein S28, mitochondrial / S28mt / 28S ribosomal protein S35 / mitochondrial / S35mt / Mitochondrial small ribosomal subunit protein bS1m

Details:

Mass: 20878.846 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9Y2Q9

#78: Protein

X6 28S ribosomal protein S29, mitochondrial / S29mt / Death-associated protein 3 / DAP-3 / Ionizing radiation resistance conferring protein / Mitochondrial small ribosomal subunit protein mS29

Details:

Mass: 45634.406 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: P51398

#79: Protein

Y6 28S ribosomal protein S31, mitochondrial / S31mt / Imogen 38 / Mitochondrial small ribosomal subunit protein mS31

Details:

Mass: 45391.473 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q92665

#80: Protein

Z6 28S ribosomal protein S33, mitochondrial / S33mt / Mitochondrial small ribosomal subunit protein mS33

Details:

Mass: 12657.776 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9Y291

#81: Protein

a6 28S ribosomal protein S34, mitochondrial / S34mt / Mitochondrial small ribosomal subunit protein mS34

Details:

Mass: 25695.549 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: P82930

#82: Protein

b6 28S ribosomal protein S35, mitochondrial / S35mt / 28S ribosomal protein S28 / mitochondrial / S28mt / Mitochondrial small ribosomal subunit protein mS35

Details:

Mass: 36898.328 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: P82673

Non-polymers , 5 types, 138 molecules

#91: Chemical

r1-801 ChemComp-GCP / PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER

Details:

Mass: 521.208 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C₁₁H₁₈N₅O₁₃P₃ / Comment: GMP-PCP, energy-carrying molecule analogue*YM

#92: Chemical

r1-802 A3-3301 A3-3302 A3-3303 A3-3304 A3-3305 A3-3306 A3-3307 A3-3308 A3-3309 A3-3310 A3-3311 A3-3312 A3-3313 A3-3314 A3-3315 A3-3316 A3-3317 A3-3318 A3-3319 ...
ChemComp-MG / MAGNESIUM ION

Details:

Mass: 24.305 Da / Num. of mol.: 128 / Source method: obtained synthetically / Formula: Mg

#93: Chemical

A3-3396 ChemComp-SPD / SPERMIDINE / N-(2-AMINO-PROPYL)-1,4-DIAMINOBUTANE / PA(34)

Details:

Mass: 145.246 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C₇H₁₉N₃

#94: Chemical

03-200 43-200 r3-200 B6-300 O6-300 P6-200 T6-200
ChemComp-ZN / ZINC ION

Details:

Mass: 65.409 Da / Num. of mol.: 7 / Source method: obtained synthetically / Formula: Zn

#95: Chemical

X6-500 ChemComp-GDP / GUANOSINE-5'-DIPHOSPHATE

Details:

Type: RNA linking / Mass: 443.201 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C₁₀H₁₅N₅O₁₁P₂ / Comment: GDP, energy-carrying molecule*YM

Experimental details

Experiment

• Experiment: Method: ELECTRON MICROSCOPY
• EM experiment: Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

Sample preparation

• Component: Name: human mitoribosome / Type: RIBOSOME / Entity ID: #1-#89 / Source: NATURAL
• Source (natural): Organism: Homo sapiens (human)
• Buffer solution: pH: 7.4
• Specimen: Conc.: 0.4 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
• Vitrification: Cryogen name: ETHANE

Electron microscopy imaging

• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company
• Microscopy: Model: FEI TITAN KRIOS
• Electron gun: Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
• Electron lens: Mode: BRIGHT FIELD
• Image recording: Electron dose: 60 e/Ų / Detector mode: INTEGRATING / Film or detector model: FEI FALCON III (4k x 4k)

Processing

• EM software: Name: EPU / Category: image acquisition
• CTF correction: Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
• 3D reconstruction: Resolution: 3.7 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 19767 / Symmetry type: POINT
• Refinement: Cross valid method: NONE; Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
• Displacement parameters: B_iso mean: 68.61 Ų

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
ELECTRON MICROSCOPY	f_bond_d	0.0137	180225
ELECTRON MICROSCOPY	f_angle_d	1.2144	256164
ELECTRON MICROSCOPY	f_chiral_restr	0.0627	30307
ELECTRON MICROSCOPY	f_plane_restr	0.0086	23037
ELECTRON MICROSCOPY	f_dihedral_angle_d	23.3372	47346

• Refine LS restraints NCS: Type: NCS constraints / Rms dev position: 0.000697267250097 Å