- EMDB-11316: Map of the shoulder domain from dynactin to resolve the upper pad... -
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Basic information
Entry
Database: EMDB / ID: EMD-11316
Title
Map of the shoulder domain from dynactin to resolve the upper paddle and hook regions
Map data
Map fit using chimera to D_1292109773 , based on regions consisting of upper paddle and hook domains and lower arm (see additional map). Map is filtered to 6 Angstroms
Sample
Complex: Dynactin complex
Biological species
Sus scrofa (pig)
Method
single particle reconstruction / cryo EM / Resolution: 4.63 Å
Journal: EMBO J / Year: 2021 Title: Cryo-EM reveals the complex architecture of dynactin's shoulder region and pointed end. Authors: Clinton K Lau / Francis J O'Reilly / Balaji Santhanam / Samuel E Lacey / Juri Rappsilber / Andrew P Carter / Abstract: Dynactin is a 1.1 MDa complex that activates the molecular motor dynein for ultra-processive transport along microtubules. In order to do this, it forms a tripartite complex with dynein and a coiled- ...Dynactin is a 1.1 MDa complex that activates the molecular motor dynein for ultra-processive transport along microtubules. In order to do this, it forms a tripartite complex with dynein and a coiled-coil adaptor. Dynactin consists of an actin-related filament whose length is defined by its flexible shoulder domain. Despite previous cryo-EM structures, the molecular architecture of the shoulder and pointed end of the filament is still poorly understood due to the lack of high-resolution information in these regions. Here we combine multiple cryo-EM datasets and define precise masking strategies for particle signal subtraction and 3D classification. This overcomes domain flexibility and results in high-resolution maps into which we can build the shoulder and pointed end. The unique architecture of the shoulder securely houses the p150 subunit and positions the four identical p50 subunits in different conformations to bind dynactin's filament. The pointed end map allows us to build the first structure of p62 and reveals the molecular basis for cargo adaptor binding to different sites at the pointed end.
History
Deposition
Jul 6, 2020
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Header (metadata) release
Jul 29, 2020
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Map release
Jul 29, 2020
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Update
Apr 28, 2021
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Current status
Apr 28, 2021
Processing site: PDBe / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
Download / File: emd_11316.map.gz / Format: CCP4 / Size: 307.5 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation
Map fit using chimera to D_1292109773 , based on regions consisting of upper paddle and hook domains and lower arm (see additional map). Map is filtered to 6 Angstroms
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