- EMDB-1080: A 11.5 A single particle reconstruction of GroEL using EMAN. -
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基本情報
登録情報
データベース: EMDB / ID: EMD-1080
タイトル
A 11.5 A single particle reconstruction of GroEL using EMAN.
マップデータ
This is the volumetric data for our published 11.5 A resolution reconstruction of GroEL. Note that newer EMAN releases can refine the same data to ~9 A resolution.
ジャーナル: J Mol Biol / 年: 2001 タイトル: A 11.5 A single particle reconstruction of GroEL using EMAN. 著者: S J Ludtke / J Jakana / J L Song / D T Chuang / W Chiu / 要旨: Single-particle analysis has become an increasingly important method for structural determination of large macromolecular assemblies. GroEL is an 800 kDa molecular chaperone, which, along with its co- ...Single-particle analysis has become an increasingly important method for structural determination of large macromolecular assemblies. GroEL is an 800 kDa molecular chaperone, which, along with its co-chaperonin GroES, promotes protein folding both in vitro and in the bacterial cell. EMAN is a single-particle analysis software package, which was first publicly distributed in 2000. We present a three-dimensional reconstruction of native naked GroEL to approximately 11.5 A performed entirely with EMAN. We demonstrate that the single-particle reconstruction, X-ray scattering data and X-ray crystal structure all agree well at this resolution. These results validate the specific methods of image restoration, reconstruction and evaluation techniques implemented in EMAN. It also demonstrates that the single-particle reconstruction technique and X-ray crystallography will yield consistent structure factors, even at low resolution, when image restoration is performed correctly. A detailed comparison of the single-particle and X-ray structures exhibits some small variations in the equatorial domain of the molecule, likely due to the absence of crystal packing forces in the single-particle reconstruction.
ダウンロード / ファイル: emd_1080.map.gz / 形式: CCP4 / 大きさ: 3.7 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈
This is the volumetric data for our published 11.5 A resolution reconstruction of GroEL. Note that newer EMAN releases can refine the same data to ~9 A resolution.
ボクセルのサイズ
X=Y=Z: 2.7 Å
密度
表面レベル
1: 0.852 / ムービー #1: 1
最小 - 最大
-1.34116 - 2.36503
平均 (標準偏差)
0.0813288 (±0.324536)
対称性
空間群: 1
詳細
EMDB XML:
マップ形状
Axis order
X
Y
Z
Origin
-50
-50
-50
サイズ
100
100
100
Spacing
100
100
100
セル
A=B=C: 267.3 Å α=β=γ: 90 °
CCP4マップ ヘッダ情報:
mode
Image stored as Reals
Å/pix. X/Y/Z
2.7
2.7
2.7
M x/y/z
99
99
99
origin x/y/z
0.000
0.000
0.000
length x/y/z
267.300
267.300
267.300
α/β/γ
90.000
90.000
90.000
start NX/NY/NZ
-30
-20
-59
NX/NY/NZ
181
231
119
MAP C/R/S
1
2
3
start NC/NR/NS
-50
-50
-50
NC/NR/NS
100
100
100
D min/max/mean
-1.341
2.365
0.081
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添付データ
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試料の構成要素
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全体 : native naked GroEL
全体
名称: native naked GroEL
要素
試料: native naked GroEL
タンパク質・ペプチド: GroEL
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超分子 #1000: native naked GroEL
超分子
名称: native naked GroEL / タイプ: sample / ID: 1000 / 集合状態: homo 14-mer / Number unique components: 1