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- EMDB-10573: Ovine ATP synthase 1a state -

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Open data

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Basic information

Entry

Database: EMDB / ID: EMD-10573

Title

Ovine ATP synthase 1a state

Map data

Composite map of state 1a of ovine F1Fo, obtained from series of focused refinements as described in the paper.

Sample

Complex: Purified ATP synthase from sheep heart mitochondria
- Protein or peptide: x 17 types
Ligand: x 6 types

Function / homology

Function and homology information

negative regulation of cell adhesion involved in substrate-bound cell migration / ATP biosynthetic process /

angiostatin binding / Formation of ATP by chemiosmotic coupling / Cristae formation / mitochondrial proton-transporting ATP synthase complex assembly / cellular response to interleukin-7 / mitochondrial proton-transporting ATP synthase complex, coupling factor F(o) / mitochondrial proton-transporting ATP synthase complex / mitochondrial proton-transporting ATP synthase complex, catalytic sector F(1) ...
Similarity search - Function

ATP synthase membrane subunit K / ATP synthase regulation /

ATP synthase, F0 complex, subunit G, mitochondrial /

ATP synthase, F0 complex, subunit E, mitochondrial /

ATP synthase subunit ATP5MJ, mitochondrial / Mitochondrial ATP synthase subunit g, animal / Mitochondrial ATP synthase g subunit / ATP synthase E chain / Mitochondrial proteolipid /

ATP synthase protein 8, metazoa ...ATP synthase membrane subunit K / ATP synthase regulation /

ATP synthase, F0 complex, subunit G, mitochondrial /

ATP synthase, F0 complex, subunit E, mitochondrial /

ATP synthase subunit ATP5MJ, mitochondrial / Mitochondrial ATP synthase subunit g, animal / Mitochondrial ATP synthase g subunit / ATP synthase E chain / Mitochondrial proteolipid /

ATP synthase protein 8, metazoa / Mitochondrial F1-F0 ATP synthase subunit F, predicted /

ATP synthase protein 8, mammals /

ATP synthase protein 8 / Mitochondrial F1F0-ATP synthase, subunit f / ATP synthase-coupling factor 6, mitochondrial / ATP synthase-coupling factor 6 superfamily, mitochondrial / Mitochondrial ATP synthase coupling factor 6 / ATP synthase delta/epsilon subunit, C-terminal domain superfamily /

ATP synthase, F0 complex, subunit B/MI25 /

ATP synthase, F0 complex, subunit B / Mitochondrial ATP synthase B chain precursor (ATP-synt_B) /

ATP synthase, F0 complex, subunit D, mitochondrial / ATP synthase D chain, mitochondrial (ATP5H) /

ATP synthase, F0 complex, subunit D superfamily, mitochondrial /

ATP synthase, F0 complex, subunit A, bacterial/mitochondria /

ATP synthase, F1 complex, epsilon subunit, mitochondrial /

ATP synthase, F1 complex, epsilon subunit superfamily, mitochondrial / Mitochondrial ATP synthase epsilon chain /

ATPase, OSCP/delta subunit, conserved site / ATP synthase delta (OSCP) subunit signature. / F1F0 ATP synthase OSCP/delta subunit, N-terminal domain superfamily /

ATP synthase, F0 complex, subunit A /

ATP synthase, F0 complex, subunit A, active site /

ATP synthase, F0 complex, subunit A superfamily / ATP synthase A chain / ATP synthase a subunit signature. /

ATPase, OSCP/delta subunit / ATP synthase delta (OSCP) subunit /

ATP synthase, F1 complex, delta/epsilon subunit /

ATP synthase, F1 complex, delta/epsilon subunit, N-terminal / F0F1 ATP synthase delta/epsilon subunit, N-terminal /

ATP synthase, Delta/Epsilon chain, beta-sandwich domain /

ATP synthase, F0 complex, subunit C / F1F0 ATP synthase subunit C superfamily /

ATP synthase, F0 complex, subunit C, DCCD-binding site / ATP synthase c subunit signature. /

ATP synthase, F1 complex, gamma subunit conserved site / ATP synthase gamma subunit signature. /

ATP synthase, F1 complex, beta subunit /

ATP synthase, alpha subunit, C-terminal domain superfamily /

ATP synthase, F1 complex, gamma subunit /

ATP synthase, F1 complex, gamma subunit superfamily /

ATP synthase /

ATP synthase, alpha subunit, C-terminal /

ATP synthase, F1 complex, alpha subunit /

ATP synthase, F1 complex, alpha subunit nucleotide-binding domain / ATP synthase alpha/beta chain, C terminal domain / V-ATPase proteolipid subunit C-like domain / F/V-ATP synthase subunit C superfamily /

ATP synthase subunit C /

ATPase, F1/V1 complex, beta/alpha subunit, C-terminal /

ATP synthase subunit alpha, N-terminal domain-like superfamily /

ATPase, F1/V1/A1 complex, alpha/beta subunit, N-terminal domain superfamily /

ATPase, F1/V1/A1 complex, alpha/beta subunit, N-terminal domain / ATP synthase alpha/beta family, beta-barrel domain /

ATPase, alpha/beta subunit, nucleotide-binding domain, active site / ATP synthase alpha and beta subunits signature. /

ATPase, F1/V1/A1 complex, alpha/beta subunit, nucleotide-binding domain / ATP synthase alpha/beta family, nucleotide-binding domain / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology

Biological species

Ovis aries (sheep) /

Sheep (sheep)

Method

single particle reconstruction /

cryo EM / Resolution: 3.5 Å

Authors

Pinke G / Zhou L / Sazanov LA

Citation

Journal: Nat Struct Mol Biol / Year: 2020
Title: Cryo-EM structure of the entire mammalian F-type ATP synthase.
Authors: Gergely Pinke / Long Zhou / Leonid A Sazanov /

Abstract: The majority of adenosine triphosphate (ATP) powering cellular processes in eukaryotes is produced by the mitochondrial F1Fo ATP synthase. Here, we present the atomic models of the membrane Fo domain ...

History

Deposition	Dec 23, 2019	-
Header (metadata) release	Sep 23, 2020	-
Map release	Sep 23, 2020	-
Update	Dec 30, 2020	-
Current status	Dec 30, 2020	Processing site: PDBe / Status: Released

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Structure visualization

Movie	Surface view with section colored by density value Surface level: 0.035 Imaged by UCSF Chimera Download Surface view colored by cylindrical radius Surface level: 0.035 Imaged by UCSF Chimera Download Surface view with fitted model Atomic models: PDB-6tt7 Surface level: 0.035 Imaged by UCSF Chimera Download Movie viewer
Structure viewer	EM map: SurfViewMolmilJmol/JSmol
Supplemental images	emd_10573.png

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Downloads & links

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EMDB archive

Map data	emd_10573.map.gz	3.2 MB		EMDB map data format
Header (meta data)	emd-10573-v30.xml emd-10573.xml	30.3 KB 30.3 KB	Display Display	EMDB header
FSC (resolution estimation)	emd_10573_fsc.xml	15.3 KB	Display	FSC data file
Images	emd_10573.png	129.8 KB
Masks	emd_10573_msk_1.map	307.5 MB		Mask map
Others	emd_10573_additional_1.map.gz emd_10573_additional_2.map.gz emd_10573_additional_3.map.gz emd_10573_half_map_1.map.gz emd_10573_half_map_2.map.gz	245.5 MB 245.6 MB 1.5 MB 245.9 MB 245.7 MB
Archive directory	http://ftp.pdbj.org/pub/emdb/structures/EMD-10573 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-10573	HTTPS FTP

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Related structure data

Related structure data	6tt7MC 11127C 6za9C M: atomic model generated by this map C: citing same article (ref.)
Similar structure data	6zqm-d 6tt7-d 6zqn-d 6zpo-d 4101 6wkr-d 4100 3098 3zia-d 11368 Search similar-shape structures by Omokage search (details) Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

EMDB pages	EMDB (EBI/PDBe) / EMDataResource
Related items in Molecule of the Month	#219 - Mar 2018 Vacuolar ATPase similarity (58) #72 - Dec 2005 ATP Synthase similarity (49) #131 - Nov 2010 Inteins similarity (10)

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Map

File

Download / File: emd_10573.map.gz / Format: CCP4 / Size: 16.8 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)

Annotation

Composite map of state 1a of ovine F1Fo, obtained from series of focused refinements as described in the paper.

Voxel size

X=Y=Z: 1.061 Å

Density

Contour Level	By AUTHOR: 0.035 / Movie #1: 0.035
Minimum - Maximum	0.0 - 0.19801688
Average (Standard dev.)	0.0052001504 (±0.015188017)

Symmetry

Space group: 1

Details

EMDB XML:

Map geometry

Axis order	Z	Y	X
Origin	0	0	0
Dimensions	139	230	138
Spacing	138	139	230

Cell

A: 146.418 Å / B: 147.479 Å / C: 244.03 Å
α=β=γ: 90.0 °

CCP4 map header:

mode	Image stored as Reals
Å/pix. X/Y/Z	1.061	1.061	1.061
M x/y/z	138	139	230
origin x/y/z	0.000	0.000	0.000
length x/y/z	146.418	147.479	244.030
α/β/γ	90.000	90.000	90.000
start NX/NY/NZ	0	0	0
NX/NY/NZ	138	139	230
MAP C/R/S	3	2	1
start NC/NR/NS	0	0	0
NC/NR/NS	230	139	138
D min/max/mean	0.000	0.198	0.005

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Supplemental data

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Mask #1

File

emd_10573_msk_1.map

Projections & Slices

Axes	Z	Y	X
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Slices (1/2)

Density Histograms

Histogram

Histogram (log scale)

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Supplemental map: emd 10573 additional 1.map

File

emd_10573_additional_1.map

Projections & Slices

Axes	Z	Y	X
Projections
Slices (1/2)

Density Histograms

Histogram

Histogram (log scale)

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Supplemental map: emd 10573 additional 2.map

File

emd_10573_additional_2.map

Projections & Slices

Axes	Z	Y	X
Projections
Slices (1/2)

Density Histograms

Histogram

Histogram (log scale)

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Supplemental map: emd 10573 additional 3.map

File

emd_10573_additional_3.map

Projections & Slices

Axes	Z	Y	X
Projections
Slices (1/2)

Density Histograms

Histogram

Histogram (log scale)

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Supplemental map: emd 10573 half map 1.map

File

emd_10573_half_map_1.map

Projections & Slices

Axes	Z	Y	X
Projections
Slices (1/2)

Density Histograms

Histogram

Histogram (log scale)

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Supplemental map: emd 10573 half map 2.map

File

emd_10573_half_map_2.map

Projections & Slices

Axes	Z	Y	X
Projections
Slices (1/2)

Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Purified ATP synthase from sheep heart mitochondria

Entire	Name: Purified ATP synthase from sheep heart mitochondria
Components	Complex: Purified ATP synthase from sheep heart mitochondria Protein or peptide: ATP synthase subunit alpha Protein or peptide: ATP synthase subunit beta Protein or peptide: ATP synthase subunit gamma Protein or peptide: ATP synthase F1 subunit delta Protein or peptide: ATP synthase F1 subunit epsilon Protein or peptide: ATP synthase peripheral stalk subunit OSCP Protein or peptide: ATP synthase subunit b Protein or peptide: ATP synthase-coupling factor 6, mitochondrial Protein or peptide: ATP synthase subunit d, mitochondrial Protein or peptide: ATP synthase F(0) complex subunit C1, mitochondrial Protein or peptide: ATP synthase subunit a Protein or peptide: Subunit DAPIT Protein or peptide: ATP synthase membrane subunit 6.8PL Protein or peptide: ATP synthase protein 8 Protein or peptide: ATP synthase membrane subunit f Protein or peptide: ATP synthase subunit Protein or peptide: Subunit e Ligand: MAGNESIUM ION Ligand: ADENOSINE-5'-DIPHOSPHATE Ligand: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE Ligand: O-[(R)-{[(2R)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-L-serine Ligand: CARDIOLIPIN Ligand: O-[(S)-hydroxy{[(2S)-2-hydroxy-3-(octadec-9-enoyloxy)propyl]oxy}phosphoryl]-L-serine

Entire

Name: Purified ATP synthase from sheep heart mitochondria

Components

Complex: Purified ATP synthase from sheep heart mitochondria
- Protein or peptide: ATP synthase subunit alpha
- Protein or peptide: ATP synthase subunit beta
- Protein or peptide: ATP synthase subunit gamma
- Protein or peptide: ATP synthase F1 subunit delta
- Protein or peptide: ATP synthase F1 subunit epsilon
- Protein or peptide: ATP synthase peripheral stalk subunit OSCP
- Protein or peptide: ATP synthase subunit b
- Protein or peptide: ATP synthase-coupling factor 6, mitochondrial
- Protein or peptide: ATP synthase subunit d, mitochondrial
- Protein or peptide: ATP synthase F(0) complex subunit C1, mitochondrial
- Protein or peptide: ATP synthase subunit a
- Protein or peptide: Subunit DAPIT
- Protein or peptide: ATP synthase membrane subunit 6.8PL
- Protein or peptide: ATP synthase protein 8
- Protein or peptide: ATP synthase membrane subunit f
- Protein or peptide: ATP synthase subunit
- Protein or peptide: Subunit e
Ligand: MAGNESIUM ION
Ligand: ADENOSINE-5'-DIPHOSPHATE
Ligand: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE
Ligand: O-[(R)-{[(2R)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-L-serine
Ligand: CARDIOLIPIN
Ligand: O-[(S)-hydroxy{[(2S)-2-hydroxy-3-(octadec-9-enoyloxy)propyl]oxy}phosphoryl]-L-serine

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Supramolecule #1: Purified ATP synthase from sheep heart mitochondria

Supramolecule	Name: Purified ATP synthase from sheep heart mitochondria / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#17
Source (natural)	Organism: Ovis aries (sheep)

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Macromolecule #1: ATP synthase subunit alpha

Macromolecule	Name: ATP synthase subunit alpha / type: protein_or_peptide / ID: 1 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)	Organism: Sheep (sheep)
Molecular weight	Theoretical: 59.779492 KDa
Sequence	String: MLSVRVAAAV ARALPRRAGL VSKNALGSSF IAARNLHASN SRLQKTGTAE VSSILEERIL GADTSVDLEE TGRVLSIGDG IARVHGLRN VQAEEMVEFS SGLKGMSLNL EPDNVGVVVF GNDKLIKEGD IVKRTGAIVD VPVGEELLGR VVDALGNAID G KGPIGSKA ...String: MLSVRVAAAV ARALPRRAGL VSKNALGSSF IAARNLHASN SRLQKTGTAE VSSILEERIL GADTSVDLEE TGRVLSIGDG IARVHGLRN VQAEEMVEFS SGLKGMSLNL EPDNVGVVVF GNDKLIKEGD IVKRTGAIVD VPVGEELLGR VVDALGNAID G KGPIGSKA RRRVGLKAPG IIPRISVREP MQTGIKAVDS LVPIGRGQRE LIIGDRQTGK TSIAIDTIIN QKRFNDGTDE KK KLYCIYV AIGQKRSTVA QLVKRLTDAD AMKYTIVVSA TASDAAPLQY LAPYSGCSMG EYFRDNGKHA LIIYDDLSKQ AVA YRQMSL LLRRPPGREA YPGDVFYLHS RLLERAAKMN DAFGGGSLTA LPVIETQAGD VSAYIPTNVI SITDGQIFLE TELF YKGIR PAINVGLSVS RVGSAAQTRA MKQVAGTMKL ELAQYREVAA FAQFGSDLDA ATQQLLSRGV RLTELLKQGQ YSPMA IEEQ VAVIYAGVRG YLDKLEPSKI TKFENAFLSH VISQHQTLLG KIRTDGKISE EADAKLKEIV TNFLAGFEA

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Macromolecule #2: ATP synthase subunit beta

Macromolecule	Name: ATP synthase subunit beta / type: protein_or_peptide / ID: 2 / Number of copies: 3 / Enantiomer: LEVO / EC number: H+-transporting two-sector ATPase
Source (natural)	Organism: Sheep (sheep)
Molecular weight	Theoretical: 56.26509 KDa
Sequence	String: MLGLVGRVAA ASASGALRGV NPSAPLPQAQ LLLRAAPAAL QPARDYAAQA SPSPKAGGAT GRIVAVIGAV VDVQFDEGLP PILNALEVQ GRETRLVLEV AQHLGESTVR TIAMDGTEGL VRGQKVLDSG APIRIPVGPE TLGRIMNVIG EPIDERGPIK T KQFAAIHA ...String: MLGLVGRVAA ASASGALRGV NPSAPLPQAQ LLLRAAPAAL QPARDYAAQA SPSPKAGGAT GRIVAVIGAV VDVQFDEGLP PILNALEVQ GRETRLVLEV AQHLGESTVR TIAMDGTEGL VRGQKVLDSG APIRIPVGPE TLGRIMNVIG EPIDERGPIK T KQFAAIHA EAPEFVEMSV EQEILVTGIK VVDLLAPYAK GGKIGLFGGA GVGKTVLIME LINNVAKAHG GYSVFAGVGE RT REGNDLY HEMIESGVIN LKDATSKVAL VYGQMNEPPG ARARVALTGL TVAEYFRDQE GQDVLLFIDN IFRFTQAGSE VSA LLGRIP SAVGYQPTLA TDMGTMQERI TTTKKGSITS VQAIYVPADD LTDPAPATTF AHLDATTVLS RAIAELGIYP AVDP LDSTS RIMDPNIVGS EHYDVARGVQ KILQDYKSLQ DIIAILGMDE LSEEDKLTVS RARKIQRFLS QPFQVAEVFT GHLGK LVPL KETIKGFQQI LAGEFDHLPE QAFYMVGPIE EAVAKADKLA EEHS

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Macromolecule #3: ATP synthase subunit gamma

Macromolecule	Name: ATP synthase subunit gamma / type: protein_or_peptide / ID: 3 / Details: XP_004014229.1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sheep (sheep)
Molecular weight	Theoretical: 33.133062 KDa
Sequence	String: MFSRAGVAGL SAWTVQPQWI QVRNMATLKD ITRRLKSIKN IQKITKSMKM VAAAKYARAE RELKPARVYG VGSLALYEKA DIKTPEDKK KHLIIGVSSD RGLCGAIHSS VAKQMKSEAA NLAAAGKEVK IIGVGDKIRS ILHRTHSDQF LVTFKEVGRR P PTFGDASV ...String: MFSRAGVAGL SAWTVQPQWI QVRNMATLKD ITRRLKSIKN IQKITKSMKM VAAAKYARAE RELKPARVYG VGSLALYEKA DIKTPEDKK KHLIIGVSSD RGLCGAIHSS VAKQMKSEAA NLAAAGKEVK IIGVGDKIRS ILHRTHSDQF LVTFKEVGRR P PTFGDASV IALELLNSGY EFDEGSIIFN RFRSVISYKT EEKPIFSLDT ISSAESMSIY DDIDADVLRN YQEYSLANII YY SLKESTT SEQSARMTAM DNASKNASEM IDKLTLTFNR TRQAVITKEL IEIISGAAAL E

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Macromolecule #4: ATP synthase F1 subunit delta

Macromolecule	Name: ATP synthase F1 subunit delta / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sheep (sheep)
Molecular weight	Theoretical: 17.580924 KDa
Sequence	String: MLPSALLRRP GLGRLVRQVR AYAEAAPAQA PAAGPGQMSF TFASPTQVFF NGANVRQVDV PTQTGAFGIL AAHVPTLQVL RPGLVVVHA EDGTTSKYFV SSGSVTVNAD SSVQLLAEEA VTLDMLDLGA AKANLEKAQS ELLGAADEAT RAEIQIRIEA N EALVKALE

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Macromolecule #5: ATP synthase F1 subunit epsilon

Macromolecule	Name: ATP synthase F1 subunit epsilon / type: protein_or_peptide / ID: 5 / Details: XP_027832792.1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sheep (sheep)
Molecular weight	Theoretical: 5.779863 KDa
Sequence	String: MVAYWRQAGL SYIRYSQICA KAVRDALKTE FKANAMKTSG SSIKIVKVKK E

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Macromolecule #6: ATP synthase peripheral stalk subunit OSCP

Macromolecule	Name: ATP synthase peripheral stalk subunit OSCP / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sheep (sheep)
Molecular weight	Theoretical: 23.435695 KDa
Sequence	String: MAALAVSGLS QQVRCFSTSV VRPFAKLVRP PVQIYGIEGR YATALYSAAS KQNKLEQVEK ELLRVGQILK EPKMAASLMN PYVKRSVKV KSLNDMTAKE KFSPLTSNLI NLLAENGRLN NTPAVISAFS TMMSVHRGEV PCTVTTASPL DEATLTELKT V LKSFLSKG ...String: MAALAVSGLS QQVRCFSTSV VRPFAKLVRP PVQIYGIEGR YATALYSAAS KQNKLEQVEK ELLRVGQILK EPKMAASLMN PYVKRSVKV KSLNDMTAKE KFSPLTSNLI NLLAENGRLN NTPAVISAFS TMMSVHRGEV PCTVTTASPL DEATLTELKT V LKSFLSKG QVLKLEVKID PSIMGGMIVR IGEKYVDMSA KTKIQKLSRA MREIL

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Macromolecule #7: ATP synthase subunit b

Macromolecule	Name: ATP synthase subunit b / type: protein_or_peptide / ID: 7 / Details: XP_004002367.2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sheep (sheep)
Molecular weight	Theoretical: 28.84457 KDa
Sequence	String: MLSRVVLSAA AAAAPSLKNA AFLGPGVLQA TRIFHTGQPS LAPVPPLPEH GGKVRFGLIP EEFFQFLYPK TGVTGPYVLG TGLILYLLS KEIYVITPET FSAISTIGFL VYVVKKYGAS VGEFADKLNE QKIAQLEEVK QASIKQIQDA IDMEKSQQAL V QKRHYLFD ...String: MLSRVVLSAA AAAAPSLKNA AFLGPGVLQA TRIFHTGQPS LAPVPPLPEH GGKVRFGLIP EEFFQFLYPK TGVTGPYVLG TGLILYLLS KEIYVITPET FSAISTIGFL VYVVKKYGAS VGEFADKLNE QKIAQLEEVK QASIKQIQDA IDMEKSQQAL V QKRHYLFD VQRNNIAMAL EVTYRERLHR VYREVKNRLD YHISVQNMMR QKEQEHMINW VEKHVVQSIS AQQEKETIAK CI ADLKLLA KKAQAQPVM

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Macromolecule #8: ATP synthase-coupling factor 6, mitochondrial

Macromolecule	Name: ATP synthase-coupling factor 6, mitochondrial / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sheep (sheep)
Molecular weight	Theoretical: 12.490296 KDa
Sequence	String: MILQRLFRLS SVVQSAISVS LRRNIGVTAV AFNKELDPVQ KLFVDKIREY RTKRQTSGGP VDAGPEYQQD LDRELFKLKQ MYGKADMNT FPNFTFEDPK FEVVEKPQS

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Macromolecule #9: ATP synthase subunit d, mitochondrial

Macromolecule	Name: ATP synthase subunit d, mitochondrial / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sheep (sheep)
Molecular weight	Theoretical: 18.747508 KDa
Sequence	String: MAGRKLALKT IDWVAFGEII PRNQKAVANS LKSWNETLTS RLATLPEKPP AIDWAYYKAN VTKAGLVDDF EKKFNALKVP IPEDKYTAQ VDAEEKEDVK SCAEFLIQSK TRIQEYEKEL EKMRNIIPFD QMTIEDLNEV FPETKLDKKK YPYWPHRPIE S L

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Macromolecule #10: ATP synthase F(0) complex subunit C1, mitochondrial

Macromolecule	Name: ATP synthase F(0) complex subunit C1, mitochondrial / type: protein_or_peptide / ID: 10 / Number of copies: 8 / Enantiomer: LEVO
Source (natural)	Organism: Sheep (sheep)
Molecular weight	Theoretical: 14.201577 KDa
Sequence	String: MQTTGALLIS PALIRSCTRG LIRPVSASFL SRPEIPSVQP SYSSGPLQVA RREFQTSVVS RDIDTAAKFI GAGAATVGVA GSGAGIGTV FGSLIIGYAR NPSLKQQLFS YAILGFALSE AMGLFCLMVA FLILFAM

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Macromolecule #11: ATP synthase subunit a

Macromolecule	Name: ATP synthase subunit a / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sheep (sheep)
Molecular weight	Theoretical: 24.81185 KDa
Sequence	String: MNENLFASFI TPMMFGLPLV TLIVLFPSLL FPTSNRLVNN RLISLQQWML QLVSKQMMSI HNTKGQTWAL MLMSLILFIG STNLLGLLP HSFTPTTQLS MNLGMAIPLW GGAVITGFRN KTKASLAHFL PQGTPTPLIP MLVIIETISL FIQPVALAVR L TANITAGH ...String: MNENLFASFI TPMMFGLPLV TLIVLFPSLL FPTSNRLVNN RLISLQQWML QLVSKQMMSI HNTKGQTWAL MLMSLILFIG STNLLGLLP HSFTPTTQLS MNLGMAIPLW GGAVITGFRN KTKASLAHFL PQGTPTPLIP MLVIIETISL FIQPVALAVR L TANITAGH LLIHLIGGAT LALMSINTTT ALITFIILIL LTVLEFAVAM IQAYVFTLLV SLYLHDNT

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Macromolecule #12: Subunit DAPIT

Macromolecule	Name: Subunit DAPIT / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sheep (sheep)
Molecular weight	Theoretical: 6.443579 KDa
Sequence	String: MAGPEADAQF HFTGIKKYFN SYTLTGRMNC VLATYGSIAL IVLYFKLRSK KTPAVKAT

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Macromolecule #13: ATP synthase membrane subunit 6.8PL

Macromolecule	Name: ATP synthase membrane subunit 6.8PL / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sheep (sheep)
Molecular weight	Theoretical: 6.846093 KDa
Sequence	String: MLQSLIKKVW IPMKPYYTQA YQEIWVGTGL MAYIVYKIRS ADKRSKALKA SSAAPAHGHH

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Macromolecule #14: ATP synthase protein 8

Macromolecule	Name: ATP synthase protein 8 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sheep (sheep)
Molecular weight	Theoretical: 7.918443 KDa
Sequence	String: MPQLDTSTWL TMILSMFLVL FIIFQLKISK HNFYHNPELM TTKTPKQNTP WETKWTKIYL PLSLPL

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Macromolecule #15: ATP synthase membrane subunit f

Macromolecule	Name: ATP synthase membrane subunit f / type: protein_or_peptide / ID: 15 / Details: XP_004016938.3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sheep (sheep)
Molecular weight	Theoretical: 10.315207 KDa
Sequence	String: MASVVPLKEK KLLEVKLGEL PSWILMRDFT PSGIAGAFQR GYYRYYNKYV NVKKGSIAGL SMVLAAYVFL NYCRSYKELK HERLRKYH

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Macromolecule #16: ATP synthase subunit

Macromolecule	Name: ATP synthase subunit / type: protein_or_peptide / ID: 16 / Details: XP_011950914.1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sheep (sheep)
Molecular weight	Theoretical: 11.428407 KDa
Sequence	String: MAQFVRNLAE KAPALVNAAV TYSKPRLATF WYYAKVELVP PTPAEIPTAI QSLKKIINSA KTGSFKQLTV KEALLNGLVA TEVWMWFYV GEIIGKRGII GYDV

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Macromolecule #17: Subunit e

Macromolecule	Name: Subunit e / type: protein_or_peptide / ID: 17 / Details: XP_027827162.1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Sheep (sheep)
Molecular weight	Theoretical: 8.336688 KDa
Sequence	String: MVPPVQVSPL IKLGRYSALF LGMAYGAKRY NYLKPRAEEE RRLAAEEKKK RDEQKRIERE LAEAQEDTIL K

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Macromolecule #18: MAGNESIUM ION

Macromolecule	Name: MAGNESIUM ION / type: ligand / ID: 18 / Number of copies: 3 / Formula: MG
Molecular weight	Theoretical: 24.305 Da

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Macromolecule #19: ADENOSINE-5'-DIPHOSPHATE

Macromolecule	Name: ADENOSINE-5'-DIPHOSPHATE / type: ligand / ID: 19 / Number of copies: 5 / Formula: ADP
Molecular weight	Theoretical: 427.201 Da
Chemical component information	ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM / Adenosine diphosphate

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Macromolecule #20: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE

Macromolecule	Name: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / type: ligand / ID: 20 / Number of copies: 6 / Formula: LHG
Molecular weight	Theoretical: 722.97 Da
Chemical component information	ChemComp-LHG: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM / Phosphatidylglycerol

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Macromolecule #21: O-[(R)-{[(2R)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phospho...

Macromolecule	Name: O-[(R)-{[(2R)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-L-serine type: ligand / ID: 21 / Number of copies: 1 / Formula: P5S
Molecular weight	Theoretical: 792.075 Da
Chemical component information	ChemComp-P5S: O-[(R)-{[(2R)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-L-serine / Phosphatidylserine

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Macromolecule #22: CARDIOLIPIN

Macromolecule	Name: CARDIOLIPIN / type: ligand / ID: 22 / Number of copies: 3 / Formula: CDL
Molecular weight	Theoretical: 1.464043 KDa
Chemical component information	ChemComp-CDL: CARDIOLIPIN / phospholipid*YM / Cardiolipin

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Macromolecule #23: O-[(S)-hydroxy{[(2S)-2-hydroxy-3-(octadec-9-enoyloxy)propyl]oxy}p...

Macromolecule	Name: O-[(S)-hydroxy{[(2S)-2-hydroxy-3-(octadec-9-enoyloxy)propyl]oxy}phosphoryl]-L-serine type: ligand / ID: 23 / Number of copies: 1 / Formula: S12
Molecular weight	Theoretical: 523.597 Da
Chemical component information	ChemComp-S12: O-[(S)-hydroxy{[(2S)-2-hydroxy-3-(octadec-9-enoyloxy)propyl]oxy}phosphoryl]-L-serine

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Experimental details

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Structure determination

Method	cryo EM
Processing	single particle reconstruction
Aggregation state	particle

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Sample preparation

Concentration	2.8 mg/mL
Buffer	pH: 7.4 Details: 20 mM HEPES pH7.4, 35 mM NaCl, 2 mM EDTA, 1% sucrose, 1 mM DTT, 0.1% LMNG, 0.2% CHAPS
Grid	Model: Quantifoil R0.6/1 / Material: COPPER / Mesh: 400 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Atmosphere: AIR
Vitrification	Cryogen name: ETHANE-PROPANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

Microscope	FEI TITAN KRIOS
Electron beam	Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron optics	Calibrated defocus max: 2.4 µm / Calibrated defocus min: 1.0 µm / Calibrated magnification: 131951 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm
Sample stage	Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Image recording	Film or detector model: FEI FALCON III (4k x 4k) / Detector mode: INTEGRATING / Digitization - Dimensions - Width: 4096 pixel / Digitization - Dimensions - Height: 4096 pixel / Digitization - Sampling interval: 14.0 µm / Number grids imaged: 1 / Number real images: 3053 / Average exposure time: 1.0 sec. / Average electron dose: 106.0 e/Å² / Details: 40 frames per movie
Experimental equipment	Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selection	Number selected: 809000
CTF correction	Software - Name: CTFFIND (ver. 4.1.13)
Startup model	Type of model: PDB ENTRY PDB model - PDB ID: 5ara
Initial angle assignment	Type: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.0)
Final 3D classification	Software - Name: RELION (ver. 3.0)
Final angle assignment	Type: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.0)
Final reconstruction	Applied symmetry - Point group: C₁ (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.5 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.0) / Number images used: 35000
FSC plot (resolution estimation)

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Atomic model buiding 1

Initial model	PDB ID: 5ara
Refinement	Space: REAL / Protocol: AB INITIO MODEL
Output model	PDB-6tt7: Ovine ATP synthase 1a state

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Atomic model buiding 2

Initial model	PDB ID: 5ara
Refinement	Space: REAL / Protocol: AB INITIO MODEL / Overall B value: 96
Output model	PDB-6tt7: Ovine ATP synthase 1a state

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-Mask #1

-Supplemental map: emd 10573 additional 1.map

-Supplemental map: emd 10573 additional 2.map

-Supplemental map: emd 10573 additional 3.map

-Supplemental map: emd 10573 half map 1.map

-Supplemental map: emd 10573 half map 2.map

-Sample components

+Entire : Purified ATP synthase from sheep heart mitochondria

+Supramolecule #1: Purified ATP synthase from sheep heart mitochondria

+Macromolecule #1: ATP synthase subunit alpha

+Macromolecule #2: ATP synthase subunit beta

+Macromolecule #3: ATP synthase subunit gamma

+Macromolecule #4: ATP synthase F1 subunit delta

+Macromolecule #5: ATP synthase F1 subunit epsilon

+Macromolecule #6: ATP synthase peripheral stalk subunit OSCP

+Macromolecule #7: ATP synthase subunit b

+Macromolecule #8: ATP synthase-coupling factor 6, mitochondrial

+Macromolecule #9: ATP synthase subunit d, mitochondrial

+Macromolecule #10: ATP synthase F(0) complex subunit C1, mitochondrial

+Macromolecule #11: ATP synthase subunit a

+Macromolecule #12: Subunit DAPIT

+Macromolecule #13: ATP synthase membrane subunit 6.8PL

+Macromolecule #14: ATP synthase protein 8

+Macromolecule #15: ATP synthase membrane subunit f

+Macromolecule #16: ATP synthase subunit

+Macromolecule #17: Subunit e

+Macromolecule #18: MAGNESIUM ION

+Macromolecule #19: ADENOSINE-5'-DIPHOSPHATE

+Macromolecule #20: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE

+Macromolecule #21: O-[(R)-{[(2R)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phospho...

+Macromolecule #22: CARDIOLIPIN

+Macromolecule #23: O-[(S)-hydroxy{[(2S)-2-hydroxy-3-(octadec-9-enoyloxy)propyl]oxy}p...

-Experimental details

-Structure determination

-Sample preparation

-Electron microscopy

-Image processing

-Atomic model buiding 1

-Atomic model buiding 2

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Supplemental data

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Mask #1

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Supplemental map: emd 10573 additional 1.map

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Supplemental map: emd 10573 additional 2.map

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Supplemental map: emd 10573 additional 3.map

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Supplemental map: emd 10573 half map 1.map

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Supplemental map: emd 10573 half map 2.map

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Sample components

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Entire : Purified ATP synthase from sheep heart mitochondria

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Supramolecule #1: Purified ATP synthase from sheep heart mitochondria

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Macromolecule #1: ATP synthase subunit alpha

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Macromolecule #2: ATP synthase subunit beta

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Macromolecule #3: ATP synthase subunit gamma

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Macromolecule #4: ATP synthase F1 subunit delta

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Macromolecule #5: ATP synthase F1 subunit epsilon

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Macromolecule #6: ATP synthase peripheral stalk subunit OSCP

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Macromolecule #7: ATP synthase subunit b

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Macromolecule #8: ATP synthase-coupling factor 6, mitochondrial

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Macromolecule #9: ATP synthase subunit d, mitochondrial

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Macromolecule #10: ATP synthase F(0) complex subunit C1, mitochondrial

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Macromolecule #11: ATP synthase subunit a

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Macromolecule #12: Subunit DAPIT

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Macromolecule #13: ATP synthase membrane subunit 6.8PL

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Macromolecule #14: ATP synthase protein 8

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Macromolecule #15: ATP synthase membrane subunit f

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Macromolecule #16: ATP synthase subunit

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Macromolecule #17: Subunit e

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Macromolecule #18: MAGNESIUM ION

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Macromolecule #19: ADENOSINE-5'-DIPHOSPHATE

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Macromolecule #20: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE

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Macromolecule #21: O-[(R)-{[(2R)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phospho...

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Macromolecule #22: CARDIOLIPIN

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Macromolecule #23: O-[(S)-hydroxy{[(2S)-2-hydroxy-3-(octadec-9-enoyloxy)propyl]oxy}p...

-
Experimental details

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Structure determination

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Sample preparation

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Electron microscopy

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Image processing

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Atomic model buiding 1

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Atomic model buiding 2

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