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- EMDB-10417: GroEL map obtained using the Preassis method for grid preparation -
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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-10417 | |||||||||
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Title | GroEL map obtained using the Preassis method for grid preparation | |||||||||
![]() | GroEL map obtained using the Preassis method for sample preparation | |||||||||
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Function / homology | ![]() GroEL-GroES complex / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Zhao J / Xu H / Carroni M / Zou X | |||||||||
Funding support | ![]()
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![]() | ![]() Title: A simple pressure-assisted method for MicroED specimen preparation. Authors: Jingjing Zhao / Hongyi Xu / Hugo Lebrette / Marta Carroni / Helena Taberman / Martin Högbom / Xiaodong Zou / ![]() ![]() Abstract: Micro-crystal electron diffraction (MicroED) has shown great potential for structure determination of macromolecular crystals too small for X-ray diffraction. However, specimen preparation remains a ...Micro-crystal electron diffraction (MicroED) has shown great potential for structure determination of macromolecular crystals too small for X-ray diffraction. However, specimen preparation remains a major bottleneck. Here, we report a simple method for preparing MicroED specimens, named Preassis, in which excess liquid is removed through an EM grid with the assistance of pressure. We show the ice thicknesses can be controlled by tuning the pressure in combination with EM grids with appropriate carbon hole sizes. Importantly, Preassis can handle a wide range of protein crystals grown in various buffer conditions including those with high viscosity, as well as samples with low crystal concentrations. Preassis is a simple and universal method for MicroED specimen preparation, and will significantly broaden the applications of MicroED. | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 25.2 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 15.5 KB 15.5 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 12.5 KB | Display | ![]() |
Images | ![]() | 89 KB | ||
Masks | ![]() | 163.6 MB | ![]() | |
Others | ![]() ![]() ![]() | 153.2 MB 129.3 MB 129.1 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | C: citing same article ( |
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Similar structure data |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | GroEL map obtained using the Preassis method for sample preparation | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 0.83 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Mask #1
File | ![]() | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Additional map: postprocessed unmasked GroEL map obtained using the Preassis...
File | emd_10417_additional_1.map | ||||||||||||
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Annotation | postprocessed unmasked GroEL map obtained using the Preassis method for sample preparation | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Half map 2 GroEL map obtained using the...
File | emd_10417_half_map_1.map | ||||||||||||
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Annotation | Half map 2 GroEL map obtained using the Preassis method for sample preparation | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Half map 1 GroEL map obtained using the...
File | emd_10417_half_map_2.map | ||||||||||||
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Annotation | Half map 1 GroEL map obtained using the Preassis method for sample preparation | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : GroEL
Entire | Name: GroEL![]() |
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Components |
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-Supramolecule #1: GroEL
Supramolecule | Name: GroEL / type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: ![]() ![]() ![]() |
Recombinant expression | Organism: ![]() ![]() ![]() |
-Experimental details
-Structure determination
Method | ![]() |
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Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.4 |
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Vitrification | Cryogen name: ETHANE |
Details | Specimen prepared using the novel freezing system Preassis |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD![]() |
Image recording | Film or detector model: GATAN K2 QUANTUM (4k x 4k) / Average electron dose: 40.0 e/Å2 |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |