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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-10013 | |||||||||
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Title | Cryo-ET on membrane protein | |||||||||
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Biological species | ![]() | |||||||||
Method | electron tomography / cryo EM | |||||||||
![]() | Zou XD / Zhao JJ / Xu HY / Carroni M | |||||||||
Funding support | ![]()
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![]() | ![]() Title: A simple pressure-assisted method for cryo-EM specimen preparation Authors: Zou XD / Zhao JJ / Xu HY / Carroni M | |||||||||
History |
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Structure visualization
Movie |
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Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 67.4 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 6.9 KB 6.9 KB | Display Display | ![]() |
Images | ![]() | 258.3 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 156.9 KB | Display | ![]() |
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Full document | ![]() | 156 KB | Display | |
Data in XML | ![]() | 4.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Voxel size | X=Y=Z: 13.8 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire : Na+/H+ antiporter
Entire | Name: Na+/H+ antiporter |
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Components |
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-Supramolecule #1: Na+/H+ antiporter
Supramolecule | Name: Na+/H+ antiporter / type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: ![]() |
Recombinant expression | Organism: ![]() ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | electron tomography |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 8 |
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Vitrification | Cryogen name: ETHANE |
Sectioning | Other: NO SECTIONING |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Average electron dose: 1.4 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: OTHER / Imaging mode: BRIGHT FIELD |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Final reconstruction | Algorithm: BACK PROJECTION / Number images used: 50 |
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