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- EMDB-5337: Unprocessed map of GroEL obtained with a DDD camera to compare re... -

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Basic information

Entry
Database: EMDB / ID: EMD-5337
TitleUnprocessed map of GroEL obtained with a DDD camera to compare results from cryo SPA with a CCD camera using the same grid. Initial evaluation of a Direct Detection Device detector for single particle cryo-electron microscopy.
Map dataUnprocessed map of GroEL from a DDD camera to compare to map obtained with CCD camera using same specimen.
Sample
  • Sample: GroEL
  • Protein or peptide: GroEL
KeywordsGroEL
Biological speciesEscherichia coli (E. coli)
Methodsingle particle reconstruction / cryo EM / Resolution: 6.7 Å
AuthorsMilazzo AC / Cheng A / Moeller A / Lyumkis D / Jacovetty E / Polukas J / Ellisman MH / Xuong NH / Carragher B / Potter CS
CitationJournal: J Struct Biol / Year: 2011
Title: Initial evaluation of a direct detection device detector for single particle cryo-electron microscopy.
Authors: Anna-Clare Milazzo / Anchi Cheng / Arne Moeller / Dmitry Lyumkis / Erica Jacovetty / James Polukas / Mark H Ellisman / Nguyen-Huu Xuong / Bridget Carragher / Clinton S Potter /
Abstract: We report on initial results of using a new direct detection device (DDD) for single particle reconstruction of vitreous ice embedded specimens. Images were acquired on a Tecnai F20 at 200keV and a ...We report on initial results of using a new direct detection device (DDD) for single particle reconstruction of vitreous ice embedded specimens. Images were acquired on a Tecnai F20 at 200keV and a nominal magnification of 29,000×. This camera has a significantly improved signal to noise ratio and modulation transfer function (MTF) at 200keV compared to a standard CCD camera installed on the same microscope. Control of the DDD has been integrated into Leginon, an automated data collection system. Using GroEL as a test specimen, we obtained images of ∼30K particles with the CCD and the DDD from the same specimen sample using essentially identical imaging conditions. Comparison of the maps reconstructed from the CCD images and the DDD images demonstrates the improved performance of the DDD. We also obtained a 3D reconstruction from ∼70K GroEL particles acquired using the DDD; the quality of the density map demonstrates the potential of this new recording device for cryoEM data acquisition.
History
DepositionAug 30, 2011-
Header (metadata) releaseNov 28, 2011-
Map releaseNov 29, 2011-
UpdateNov 29, 2011-
Current statusNov 29, 2011Processing site: RCSB / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.00433
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by cylindrical radius
  • Surface level: 0.00433
  • Imaged by UCSF Chimera
  • Download
Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_5337_1.png

Downloads & links

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Map

FileDownload / File: emd_5337.map.gz / Format: CCP4 / Size: 26.4 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationUnprocessed map of GroEL from a DDD camera to compare to map obtained with CCD camera using same specimen.
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.37 Å/pix.
x 192 pix.
= 263.04 Å		1.37 Å/pix.
x 192 pix.
= 263.04 Å		1.37 Å/pix.
x 192 pix.
= 263.04 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.37 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.00433 / Movie #1: 0.00433
Minimum - Maximum-0.0173935 - 0.017467
Average (Standard dev.)0.000014234 (±0.00259279)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin-96-96-96
Dimensions192192192
Spacing192192192
CellA=B=C: 263.04 Å
α=β=γ: 90 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.371.371.37
M x/y/z192192192
origin x/y/z0.0000.0000.000
length x/y/z263.040263.040263.040
α/β/γ90.00090.00090.000
start NX/NY/NZ-62-62-62
NX/NY/NZ125125125
MAP C/R/S123
start NC/NR/NS-96-96-96
NC/NR/NS192192192
D min/max/mean-0.0170.0170.000

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Supplemental data

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Sample components

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Entire : GroEL

EntireName: GroEL
Components
  • Sample: GroEL
  • Protein or peptide: GroEL

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Supramolecule #1000: GroEL

SupramoleculeName: GroEL / type: sample / ID: 1000 / Oligomeric state: D7 14-mer / Number unique components: 1
Molecular weightExperimental: 800 KDa / Theoretical: 800 KDa

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Macromolecule #1: GroEL

MacromoleculeName: GroEL / type: protein_or_peptide / ID: 1 / Name.synonym: GroEL / Number of copies: 1 / Oligomeric state: homotetradecamer / Recombinant expression: Yes / Database: NCBI
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightExperimental: 800 KDa / Theoretical: 800 KDa

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration1.5 mg/mL
BufferpH: 7.5 / Details: 100mM HEPES, 10mM Mg(OAc)2, 10mM KOAc, 2mM DTT
GridDetails: Protochips C-flat grid, holey carbon with 2um holes and 2um spacing 400 mesh copper grid
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 93 K / Instrument: OTHER
Details: Vitrification instrument: Vitrobot. Grid plasma cleaned for 20 seconds with Fischione 1020 plasma cleaner using 75 percent argon, 25 percent oxygen mix.
Method: Temperature of chamber was 4 degrees C. 0 seconds drain time. Single blot. 0 mm offset. 4 uL sample applied to grid. Blot for 3.5 seconds before plunging.

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Electron microscopy

MicroscopeFEI TECNAI F20
TemperatureMin: 102 K / Max: 102 K / Average: 102 K
Alignment procedureLegacy - Astigmatism: The objective lens astigmatism was corrected automatically using Leginon
Legacy - Electron beam tilt params: beam tilt was less than 0.1 mrad
DateFeb 24, 2011
Image recordingCategory: CCD / Film or detector model: DIRECT ELECTRON DE-12 (4k x 3k) / Number real images: 2313 / Average electron dose: 20 e/Å2 / Bits/pixel: 16
Tilt angle min0
Tilt angle max0
Electron beamAcceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
Electron opticsCalibrated magnification: 43796 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2 mm / Nominal defocus max: 2.5 µm / Nominal defocus min: 0.8 µm / Nominal magnification: 29000
Sample stageSpecimen holder: Gatan side-entry 626 cryostage / Specimen holder model: GATAN LIQUID NITROGEN
Experimental equipment
Model: Tecnai F20 / Image courtesy: FEI Company

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Image processing

DetailsThe particles were selected using an automatic selection program.
CTF correctionDetails: CTF correction of each particle
Final reconstructionAlgorithm: OTHER / Resolution.type: BY AUTHOR / Resolution: 6.7 Å / Resolution method: FSC 0.5 CUT-OFF / Software - Name: XMIPP / Number images used: 30711

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