[English] 日本語
![](img/lk-miru.gif)
- EMDB-1009: A cryo-electron microscopic study of ribosome-bound termination f... -
+
Open data
-
Basic information
Entry | Database: EMDB / ID: EMD-1009 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | A cryo-electron microscopic study of ribosome-bound termination factor RF2. | |||||||||
![]() | The RF2(GAQ) mutant cryo-density from the 3D-EM map of RF2 mutant bound to the E.coli 70s ribosome | |||||||||
![]() |
| |||||||||
Function / homology | ![]() translation release factor activity, codon specific / translational termination / small ribosomal subunit / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / tRNA binding / rRNA binding / structural constituent of ribosome / translation / cytosol Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 10.9 Å | |||||||||
![]() | Rawat UB / Zavialov AV / Sengupta J / Valle M / Grassucci RA / Linde J / Vestergaard B / Ehrenberg M / Frank J | |||||||||
![]() | ![]() Title: A cryo-electron microscopic study of ribosome-bound termination factor RF2. Authors: Urmila B S Rawat / Andrey V Zavialov / Jayati Sengupta / Mikel Valle / Robert A Grassucci / Jamie Linde / Bente Vestergaard / Måns Ehrenberg / Joachim Frank / ![]() Abstract: Protein synthesis takes place on the ribosome, where genetic information carried by messenger RNA is translated into a sequence of amino acids. This process is terminated when a stop codon moves into ...Protein synthesis takes place on the ribosome, where genetic information carried by messenger RNA is translated into a sequence of amino acids. This process is terminated when a stop codon moves into the ribosomal decoding centre (DC) and is recognized by a class-1 release factor (RF). RFs have a conserved GGQ amino-acid motif, which is crucial for peptide release and is believed to interact directly with the peptidyl-transferase centre (PTC) of the 50S ribosomal subunit. Another conserved motif of RFs (SPF in RF2) has been proposed to interact directly with stop codons in the DC of the 30S subunit. The distance between the DC and PTC is approximately 73 A. However, in the X-ray structure of RF2, SPF and GGQ are only 23 A apart, indicating that they cannot be at DC and PTC simultaneously. Here we show that RF2 is in an open conformation when bound to the ribosome, allowing GGQ to reach the PTC while still allowing SPF-stop-codon interaction. The results indicate new interpretations of accuracy in termination, and have implications for how the presence of a stop codon in the DC is signalled to PTC. | |||||||||
History |
|
-
Structure visualization
Movie |
![]() |
---|---|
Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
-
Downloads & links
-EMDB archive
Map data | ![]() | 7.4 MB | ![]() | |
---|---|---|---|---|
Header (meta data) | ![]() ![]() | 8.8 KB 8.8 KB | Display Display | ![]() |
Images | ![]() | 15.3 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 278 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 277.6 KB | Display | |
Data in XML | ![]() | 5.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
-
Links
EMDB pages | ![]() ![]() |
---|---|
Related items in Molecule of the Month |
-
Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | The RF2(GAQ) mutant cryo-density from the 3D-EM map of RF2 mutant bound to the E.coli 70s ribosome | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 2.82 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
|
-Supplemental data
-
Sample components
-Entire : RF2 (GAQ) mutant from E.coli
Entire | Name: RF2 (GAQ) mutant from E.coli |
---|---|
Components |
|
-Supramolecule #1000: RF2 (GAQ) mutant from E.coli
Supramolecule | Name: RF2 (GAQ) mutant from E.coli / type: sample / ID: 1000 Details: SPIDER was used for the computational isolation of the RF2(GAQ) mutant cryo-density from the 3D-EM map of RF2 mutant bound to the E.coli 70s ribosome (EMD-1008). Number unique components: 1 |
---|
-Macromolecule #1: RF2 (GAQ) mutant
Macromolecule | Name: RF2 (GAQ) mutant / type: protein_or_peptide / ID: 1 / Recombinant expression: Yes |
---|---|
Source (natural) | Organism: ![]() ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
---|---|
![]() | single particle reconstruction |
Aggregation state | particle |
-
Sample preparation
Vitrification | Cryogen name: ETHANE |
---|
-
Electron microscopy
Microscope | FEI TECNAI F20 |
---|---|
Temperature | Average: 93 K |
Details | information is the same as in submission EMD-1008 |
Date | Aug 2, 2001 |
Image recording | Category: FILM / Film or detector model: KODAK SO-163 FILM / Digitization - Scanner: ZEISS SCAI / Average electron dose: 20 e/Å2 / Details: entry EMD-1008 |
Tilt angle min | 0 |
Tilt angle max | 0 |
Electron beam | Acceleration voltage: 200 kV / Electron source: ![]() |
Electron optics | Calibrated magnification: 49696 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.0 mm / Nominal defocus max: 4.05 µm / Nominal defocus min: 2.25 µm / Nominal magnification: 50000 |
Sample stage | Specimen holder: Oxford, cryo-transfer 3500 / Specimen holder model: GATAN LIQUID NITROGEN |
Experimental equipment | ![]() Model: Tecnai F20 / Image courtesy: FEI Company |
-
Image processing
Details | entry EMD-1008 |
---|---|
Final reconstruction | Applied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 10.9 Å Details: information is the same as in submission 1186. SPIDER was used for the computational isolation of the RF2 (GAQ) mutant cryo-density from the 3D-EM map of RF2 mutant bound to the E.coli 70s ribosome (EMD-1008). Number images used: 1 |
-Atomic model buiding 1
Initial model | (PDB ID: , ) |
---|---|
Output model | ![]() PDB-1mi6: ![]() PDB-1mvr: |