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- EMDB-10022: Structure of human mitochondrial 28S ribosome in complex with mit... -

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Basic information

Entry
Database: EMDB / ID: EMD-10022
TitleStructure of human mitochondrial 28S ribosome in complex with mitochondrial IF2 and IF3
Map dataComposite map, local resolution filtered
Sample
  • Complex: IF2 and IF3 bound mitochondrial translation pre-initiation complex
    • Complex: Ribosome
      • RNA: x 1 types
      • Protein or peptide: x 30 types
    • Complex: IF2
      • Protein or peptide: x 1 types
    • Complex: IF3
      • Protein or peptide: x 1 types
  • Ligand: x 10 types
Keywordsribosomal small subunit / initiation complex / initiation factor / RIBOSOME
Function / homology
Function and homology information


mitochondrial translational initiation / mitochondrial ribosome binding / translation factor activity, RNA binding / mitochondrial ribosome assembly / ribosome disassembly / Mitochondrial translation elongation / Mitochondrial translation termination / Mitochondrial translation initiation / positive regulation of mitochondrial translation / negative regulation of mitotic nuclear division ...mitochondrial translational initiation / mitochondrial ribosome binding / translation factor activity, RNA binding / mitochondrial ribosome assembly / ribosome disassembly / Mitochondrial translation elongation / Mitochondrial translation termination / Mitochondrial translation initiation / positive regulation of mitochondrial translation / negative regulation of mitotic nuclear division / mitochondrial ribosome / mitochondrial small ribosomal subunit / mitochondrial translation / regulation of translational initiation / positive regulation of proteolysis / ribosomal small subunit binding / Mitochondrial protein degradation / translation initiation factor activity / apoptotic signaling pathway / fibrillar center / small ribosomal subunit rRNA binding / cell junction / ribosome binding / regulation of translation / ribosomal small subunit assembly / small ribosomal subunit / nuclear membrane / cell population proliferation / tRNA binding / mitochondrial inner membrane / rRNA binding / ribosome / structural constituent of ribosome / mitochondrial matrix / translation / protein domain specific binding / intracellular membrane-bounded organelle / GTPase activity / mRNA binding / nucleolus / GTP binding / mitochondrion / RNA binding / nucleoplasm / nucleus / plasma membrane / cytosol / cytoplasm
Similarity search - Function
Translation initiation factor 3 / Translation initiation factor 3, N-terminal / Translation initiation factor 3 (IF-3), N-terminal domain superfamily / Translation initiation factor 3 (IF-3), C-terminal domain superfamily / Translation initiation factor IF-3, N-terminal domain / Translation initiation factor IF-2, domain II / Initiation factor 2 signature. / Translation initiation factor IF-2, bacterial-like / Coiled-coil-helix-coiled-coil-helix domain-containing protein 1 / Translation initiation factor IF- 2, domain 3 ...Translation initiation factor 3 / Translation initiation factor 3, N-terminal / Translation initiation factor 3 (IF-3), N-terminal domain superfamily / Translation initiation factor 3 (IF-3), C-terminal domain superfamily / Translation initiation factor IF-3, N-terminal domain / Translation initiation factor IF-2, domain II / Initiation factor 2 signature. / Translation initiation factor IF-2, bacterial-like / Coiled-coil-helix-coiled-coil-helix domain-containing protein 1 / Translation initiation factor IF- 2, domain 3 / Translation-initiation factor 2 / : / Elongation factor G domain 2 / Translation initiation factor IF- 2 / Translation initiation factor IF-2, domain 3 superfamily / 28S ribosomal protein S26 / Mitochondrial ribosome subunit S26 / Cysteine alpha-hairpin motif superfamily / 28S ribosomal protein S27, mitochondrial / MRPS27/PTCD2 / Mitochondrial 28S ribosomal protein S27 / Ribosomal protein S22, mitochondrial / Ribosomal protein S23, mitochondrial / Ribosomal protein S23/S25, mitochondrial / Mitochondrial 28S ribosomal protein S31 / : / Mitochondrial 28S ribosomal protein S22 / Mitochondrial ribosomal protein S23 / Mitochondrial 28S ribosomal protein S31 / Ribosomal protein S29, mitochondrial / Ribosomal protein S28, mitochondrial / Mitochondrial 28S ribosomal protein S34 / 28S ribosomal protein S10, mitochondrial / Mitochondrial ribosomal protein MRP-S35 / Mitochondrial 28S ribosomal protein S34 / 28S ribosomal protein S24, mitochondrial / Pentatricopeptide repeat domain-containing protein 3 / 28S ribosomal protein S17, mitochondrial / 28S ribosomal protein S18b, mitochondrial / : / Mitochondrial ribosome subunit S24 / Small ribosomal subunit protein uS5m, N-terminal / Small ribosomal subunit protein mS39, PPR / 28S ribosomal protein S25, mitochondrial / Pentatricopeptide repeat domain / Ribosomal protein S27/S33, mitochondrial / Ribosomal protein S24/S35, mitochondrial / Mitochondrial ribosomal subunit S27 / Ribosomal protein S24/S35, mitochondrial, conserved domain / Mitochondrial ribosomal subunit protein / Pentatricopeptide (PPR) repeat profile. / Ribosomal protein S23/S29, mitochondrial / Mitochondrial ribosomal death-associated protein 3 / Pentatricopeptide repeat / Mitochondrial mRNA-processing protein COX24, C-terminal / Mitochondrial mRNA-processing protein COX24, C-terminal / Mitochondrial domain of unknown function (DUF1713) / CHCH / CHCH domain / Coiled coil-helix-coiled coil-helix (CHCH) domain profile. / Ribosomal protein/NADH dehydrogenase domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / Ribosomal protein S21 superfamily / Ribosomal protein S21 / Translational (tr)-type GTP-binding domain / Elongation factor Tu GTP binding domain / Translational (tr)-type guanine nucleotide-binding (G) domain profile. / Ribosomal protein S21 / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal protein S18, conserved site / Ribosomal protein S18 signature. / Ribosomal protein S16 / Ribosomal protein S16 domain superfamily / Ribosomal protein S16 / Ribosomal protein S15, bacterial-type / Ribosomal protein S6 / Ribosomal protein S6 / Ribosomal protein S6 superfamily / Ribosomal protein S12, bacterial-type / Translation elongation factor EF1B/ribosomal protein S6 / Ribosomal protein S18 / Ribosomal protein S18 / Ribosomal protein S18 superfamily / Ribosomal protein S2 signature 1. / Ribosomal protein S2, conserved site / Ribosomal protein S5, N-terminal, conserved site / Ribosomal protein S5 signature. / Ribosomal protein S2 / Ribosomal protein S2, flavodoxin-like domain superfamily / Ribosomal protein S2 / Ribosomal protein S5 / S5 double stranded RNA-binding domain profile. / Ribosomal protein S5, N-terminal / Ribosomal protein S5, N-terminal domain / Ribosomal protein S5, C-terminal / Ribosomal protein S5, C-terminal domain / Ribosomal protein S14 / Ribosomal protein S14p/S29e / Small GTP-binding protein domain
Similarity search - Domain/homology
Small ribosomal subunit protein uS12m / Small ribosomal subunit protein uS14m / Translation initiation factor IF-2, mitochondrial / Small ribosomal subunit protein mS29 / Small ribosomal subunit protein mS22 / Small ribosomal subunit protein mS25 / Small ribosomal subunit protein uS10m / Small ribosomal subunit protein mS35 / Small ribosomal subunit protein uS5m / Small ribosomal subunit protein uS11m ...Small ribosomal subunit protein uS12m / Small ribosomal subunit protein uS14m / Translation initiation factor IF-2, mitochondrial / Small ribosomal subunit protein mS29 / Small ribosomal subunit protein mS22 / Small ribosomal subunit protein mS25 / Small ribosomal subunit protein uS10m / Small ribosomal subunit protein mS35 / Small ribosomal subunit protein uS5m / Small ribosomal subunit protein uS11m / Small ribosomal subunit protein uS15m / Small ribosomal subunit protein bS21m / Small ribosomal subunit protein mS34 / Small ribosomal subunit protein bS6m / Small ribosomal subunit protein uS9m / Small ribosomal subunit protein mS27 / Small ribosomal subunit protein mS31 / Small ribosomal subunit protein mS37 / Small ribosomal subunit protein uS3m / Small ribosomal subunit protein mS39 / Small ribosomal subunit protein mS26 / Translation initiation factor IF-3, mitochondrial / Small ribosomal subunit protein mS38 / Small ribosomal subunit protein mS33 / Small ribosomal subunit protein bS1m / Small ribosomal subunit protein uS17m / Small ribosomal subunit protein uS7m / Small ribosomal subunit protein uS2m / Small ribosomal subunit protein bS16m / Small ribosomal subunit protein bS18m / Small ribosomal subunit protein mS23 / Small ribosomal subunit protein mS40
Similarity search - Component
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.14 Å
AuthorsItoh Y / Khawaja A
CitationJournal: Nat Commun / Year: 2020
Title: Distinct pre-initiation steps in human mitochondrial translation.
Authors: Anas Khawaja / Yuzuru Itoh / Cristina Remes / Henrik Spåhr / Olessya Yukhnovets / Henning Höfig / Alexey Amunts / Joanna Rorbach /
Abstract: Translation initiation in human mitochondria relies upon specialized mitoribosomes and initiation factors, mtIF2 and mtIF3, which have diverged from their bacterial counterparts. Here we report two ...Translation initiation in human mitochondria relies upon specialized mitoribosomes and initiation factors, mtIF2 and mtIF3, which have diverged from their bacterial counterparts. Here we report two distinct mitochondrial pre-initiation assembly steps involving those factors. Single-particle cryo-EM revealed that in the first step, interactions between mitochondria-specific protein mS37 and mtIF3 keep the small mitoribosomal subunit in a conformation favorable for a subsequent accommodation of mtIF2 in the second step. Combination with fluorescence cross-correlation spectroscopy analyses suggests that mtIF3 promotes complex assembly without mRNA or initiator tRNA binding, where exclusion is achieved by the N-terminal and C-terminal domains of mtIF3. Finally, the association of large mitoribosomal subunit is required for initiator tRNA and leaderless mRNA recruitment to form a stable initiation complex. These data reveal fundamental aspects of mammalian protein synthesis that are specific to mitochondria.
History
DepositionJun 3, 2019-
Header (metadata) releaseJun 3, 2020-
Map releaseJun 3, 2020-
UpdateApr 24, 2024-
Current statusApr 24, 2024Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.025
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.025
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6rw5
  • Surface level: 0.025
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_10022.png

Downloads & links

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Map

FileDownload / File: emd_10022.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationComposite map, local resolution filtered
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.83 Å/pix.
x 480 pix.
= 398.4 Å		0.83 Å/pix.
x 480 pix.
= 398.4 Å		0.83 Å/pix.
x 480 pix.
= 398.4 Å

Surface

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Slices (1/2)

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Images are generated by Spider.

Voxel sizeX=Y=Z: 0.83 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.025 / Movie #1: 0.025
Minimum - Maximum0.0 - 0.41354424
Average (Standard dev.)0.0007958906 (±0.007835005)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions480480480
Spacing480480480
CellA=B=C: 398.4 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z0.830.830.83
M x/y/z480480480
origin x/y/z0.0000.0000.000
length x/y/z398.400398.400398.400
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS480480480
D min/max/mean0.0000.4140.001

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Supplemental data

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Mask #1

Fileemd_10022_msk_1.map
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Additional map: Overall map without sharpening

Fileemd_10022_additional.map
AnnotationOverall map without sharpening
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Half map: #1

Fileemd_10022_half_map_1.map
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Half map: #2

Fileemd_10022_half_map_2.map
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Sample components

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Entire : IF2 and IF3 bound mitochondrial translation pre-initiation complex

EntireName: IF2 and IF3 bound mitochondrial translation pre-initiation complex
Components
  • Complex: IF2 and IF3 bound mitochondrial translation pre-initiation complex
    • Complex: Ribosome
      • RNA: 12S mitochondrial rRNA
      • Protein or peptide: 28S ribosomal protein S2, mitochondrial
      • Protein or peptide: 28S ribosomal protein S24, mitochondrial
      • Protein or peptide: 28S ribosomal protein S5, mitochondrial
      • Protein or peptide: 28S ribosomal protein S6, mitochondrial
      • Protein or peptide: 28S ribosomal protein S7, mitochondrial
      • Protein or peptide: 28S ribosomal protein S9, mitochondrial
      • Protein or peptide: 28S ribosomal protein S10, mitochondrial
      • Protein or peptide: 28S ribosomal protein S11, mitochondrial
      • Protein or peptide: 28S ribosomal protein S12, mitochondrial
      • Protein or peptide: 28S ribosomal protein S14, mitochondrial
      • Protein or peptide: 28S ribosomal protein S15, mitochondrial
      • Protein or peptide: 28S ribosomal protein S16, mitochondrial
      • Protein or peptide: 28S ribosomal protein S17, mitochondrial
      • Protein or peptide: 28S ribosomal protein S18b, mitochondrial
      • Protein or peptide: 28S ribosomal protein S18c, mitochondrial
      • Protein or peptide: 28S ribosomal protein S21, mitochondrial
      • Protein or peptide: 28S ribosomal protein S22, mitochondrial
      • Protein or peptide: 28S ribosomal protein S23, mitochondrial
      • Protein or peptide: 28S ribosomal protein S25, mitochondrial
      • Protein or peptide: 28S ribosomal protein S26, mitochondrial
      • Protein or peptide: 28S ribosomal protein S27, mitochondrial
      • Protein or peptide: 28S ribosomal protein S28, mitochondrial
      • Protein or peptide: 28S ribosomal protein S29, mitochondrial
      • Protein or peptide: 28S ribosomal protein S31, mitochondrial
      • Protein or peptide: 28S ribosomal protein S33, mitochondrial
      • Protein or peptide: 28S ribosomal protein S34, mitochondrial
      • Protein or peptide: 28S ribosomal protein S35, mitochondrial
      • Protein or peptide: Coiled-coil-helix-coiled-coil-helix domain-containing protein 1
      • Protein or peptide: Aurora kinase A-interacting protein
      • Protein or peptide: Pentatricopeptide repeat domain-containing protein 3, mitochondrial
    • Complex: IF2
      • Protein or peptide: Translation initiation factor IF-2, mitochondrial
    • Complex: IF3
      • Protein or peptide: Translation initiation factor IF-3, mitochondrial
  • Ligand: NICOTINAMIDE-ADENINE-DINUCLEOTIDE
  • Ligand: SPERMINE
  • Ligand: STREPTOMYCIN
  • Ligand: MAGNESIUM ION
  • Ligand: POTASSIUM ION
  • Ligand: ZINC ION
  • Ligand: FE2/S2 (INORGANIC) CLUSTER
  • Ligand: ADENOSINE-5'-TRIPHOSPHATE
  • Ligand: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER
  • Ligand: water

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Supramolecule #1: IF2 and IF3 bound mitochondrial translation pre-initiation complex

SupramoleculeName: IF2 and IF3 bound mitochondrial translation pre-initiation complex
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#33
Details: Small subunit of mitochondrial ribosome in complex with mitochondrial IF2 and IF3
Molecular weightTheoretical: 1.1 MDa

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Supramolecule #2: Ribosome

SupramoleculeName: Ribosome / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1-#31
Source (natural)Organism: Homo sapiens (human)

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Supramolecule #3: IF2

SupramoleculeName: IF2 / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #32
Source (natural)Organism: Homo sapiens (human)

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Supramolecule #4: IF3

SupramoleculeName: IF3 / type: complex / ID: 4 / Parent: 1 / Macromolecule list: #33
Source (natural)Organism: Homo sapiens (human)

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Macromolecule #1: 12S mitochondrial rRNA

MacromoleculeName: 12S mitochondrial rRNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 306.547531 KDa
SequenceString: AAUAGGUUUG GUCCUAGCCU UUCUAUUAGC UCUUAGUAAG AUUACACAUG CAAGCAUCCC CGUUCCAGUG AGUUCACCCU CUAAAUCAC CACGAUCAAA AGGGACAAGC AUCAAGCACG CAGCAAUGCA GCUCAAAACG CUUAGCCUAG CCACACCCCC A CGGGAAAC ...String:
AAUAGGUUUG GUCCUAGCCU UUCUAUUAGC UCUUAGUAAG AUUACACAUG CAAGCAUCCC CGUUCCAGUG AGUUCACCCU CUAAAUCAC CACGAUCAAA AGGGACAAGC AUCAAGCACG CAGCAAUGCA GCUCAAAACG CUUAGCCUAG CCACACCCCC A CGGGAAAC AGCAGUGAUU AACCUUUAGC AAUAAACGAA AGUUUAACUA AGCUAUACUA ACCCCAGGGU UGGUCAAUUU CG UGCCAGC CACCGCGGUC ACACGAUUAA CCCAAGUCAA UAGAAGCCGG CGUAAAGAGU GUUUUAGAUC ACCCCCUCCC CAA UAAAGC UAAAACUCAC CUGAGUUGUA AAAAACUCCA GUUGACACAA AAUAGACUAC GAAAGUGGCU UUAACAUAUC UGAA CACAC AAUAGCUAAG ACCCAAACUG GGA(5MU)UAGAUA CCCCACUAUG CUUAGCCCUA AACCUCAACA GUUAAAUCAA C AAAACUGC UCGCCAGAAC ACUACGAGCC ACAGCUUAAA ACUCAAAGGA CCUGGCGGUG CUUCAUAUCC CUCUAGAGGA GC CUGUUCU GUAAUCGAUA AACCCCGAUC AACCUCACCA CCUCUUGCUC AGCCUAUAUA CCGCCAUCUU CAGCAAACCC UGA UGAAGG CUACAAAGUA AGCGCAAGUA CCCACGUAAA GACGUUAGGU CAAGGUGUAG CCCAUGAGGU GGCAAGAAAU GGGC UACAU UUUCUACCCC AGAAAACUAC GAUAGCCCUU AUGAAACUUA AGGGUCGAAG GUGGAUUUAG CAGUAAACUG AGAGU AGAG UGCUUAGUUG AACAGGGCCC UGAAGCGCGU ACACACCG(B8T)C (5MC)GUCACCCUC CUCAAGUAUA CUUCAAAG G ACAUUUAACU AAAACCCCUA CGCAUUUAUA UAGAGGAGAC AAGUCGUAAC AUGGUAAGUG UACUGG(MA6)(MA6)AG U GCACUUGG ACGAACC

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Macromolecule #2: 28S ribosomal protein S2, mitochondrial

MacromoleculeName: 28S ribosomal protein S2, mitochondrial / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 33.298238 KDa
SequenceString: MATSSAALPR ILGAGARAPS RWLGFLGKAT PRPARPSRRT LGSATALMIR ESEDSTDFND KILNEPLKHS DFFNVKELFS VRSLFDARV HLGHKAGCRH RFMEPYIFGS RLDHDIIDLE QTATHLQLAL NFTAHMAYRK GIILFISRNR QFSYLIENMA R DCGEYAHT ...String:
MATSSAALPR ILGAGARAPS RWLGFLGKAT PRPARPSRRT LGSATALMIR ESEDSTDFND KILNEPLKHS DFFNVKELFS VRSLFDARV HLGHKAGCRH RFMEPYIFGS RLDHDIIDLE QTATHLQLAL NFTAHMAYRK GIILFISRNR QFSYLIENMA R DCGEYAHT RYFRGGMLTN ARLLFGPTVR LPDLIIFLHT LNNIFEPHVA VRDAAKMNIP TVGIVDTNCN PCLITYPVPG ND DSPLAVH LYCRLFQTAI TRAKEKRQQV EALYRLQGQK EPGDQGPAHP PGADMSHSL

UniProtKB: Small ribosomal subunit protein uS2m

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Macromolecule #3: 28S ribosomal protein S24, mitochondrial

MacromoleculeName: 28S ribosomal protein S24, mitochondrial / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 19.046164 KDa
SequenceString:
MAASVCSGLL GPRVLSWSRE LPCAWRALHT SPVCAKNRAA RVRVSKGDKP VTYEEAHAPH YIAHRKGWLS LHTGNLDGED HAAERTVED VFLRKFMWGT FPGCLADQLV LKRRGNQLEI CAVVLRQLSP HKYYFLVGYS ETLLSYFYKC PVRLHLQTVP S KVVYKYL

UniProtKB: Small ribosomal subunit protein uS3m

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Macromolecule #4: 28S ribosomal protein S5, mitochondrial

MacromoleculeName: 28S ribosomal protein S5, mitochondrial / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 48.094594 KDa
SequenceString: MATAVRAVGC LPVLCSGTAG HLLGRQCSLN TLPAASILAW KSVLGNGHLS SLGTRDTHPY ASLSRALQTQ CCISSPSHLM SQQYRPYSF FTKLTADELW KGALAETGAG AKKGRGKRTK KKKRKDLNRG QIIGEGRYGF LWPGLNVPLM KNGAVQTIAQ R SKEEQEKV ...String:
MATAVRAVGC LPVLCSGTAG HLLGRQCSLN TLPAASILAW KSVLGNGHLS SLGTRDTHPY ASLSRALQTQ CCISSPSHLM SQQYRPYSF FTKLTADELW KGALAETGAG AKKGRGKRTK KKKRKDLNRG QIIGEGRYGF LWPGLNVPLM KNGAVQTIAQ R SKEEQEKV EADMIQQREE WDRKKKMKVK RERGWSGNSW GGISLGPPDP GPCGETYEDF DTRILEVRNV FTMTAKEGRK KS IRVLVAV GNGKGAAGFS IGKATDRMDA FRKAKNRAVH HLHYIERYED HTIFHDISLR FKRTHIKMKK QPKGYGLRCH RAI ITICRL IGIKDMYAKV SGSINMLSLT QGLFRGLSRQ ETHQQLADKK GLHVVEIREE CGPLPIVVAS PRGPLRKDPE PEDE VPDVK LDWEDVKTAQ GMKRSVWSNL KRAAT

UniProtKB: Small ribosomal subunit protein uS5m

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Macromolecule #5: 28S ribosomal protein S6, mitochondrial

MacromoleculeName: 28S ribosomal protein S6, mitochondrial / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 14.250588 KDa
SequenceString:
MPRYELALIL KAMQRPETAA TLKRTIEALM DRGAIVRDLE NLGERALPYR ISAHSQQHNR GGYFLVDFYA PTAAVESMVE HLSRDIDVI RGNIVKHPLT QELKECEGIV PVPLAEKLYS TKKRKK

UniProtKB: Small ribosomal subunit protein bS6m

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Macromolecule #6: 28S ribosomal protein S7, mitochondrial

MacromoleculeName: 28S ribosomal protein S7, mitochondrial / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 28.186154 KDa
SequenceString: MAAPAVKVAR GWSGLALGVR RAVLQLPGLT QVRWSRYSPE FKDPLIDKEY YRKPVEELTE EEKYVRELKK TQLIKAAPAG KTSSVFEDP VISKFTNMMM IGGNKVLARS LMIQTLEAVK RKQFEKYHAA SAEEQATIER NPYTIFHQAL KNCEPMIGLV P ILKGGRFY ...String:
MAAPAVKVAR GWSGLALGVR RAVLQLPGLT QVRWSRYSPE FKDPLIDKEY YRKPVEELTE EEKYVRELKK TQLIKAAPAG KTSSVFEDP VISKFTNMMM IGGNKVLARS LMIQTLEAVK RKQFEKYHAA SAEEQATIER NPYTIFHQAL KNCEPMIGLV P ILKGGRFY QVPVPLPDRR RRFLAMKWMI TECRDKKHQR TLMPEKLSHK LLEAFHNQGP VIKRKHDLHK MAEANRALAH YR WW

UniProtKB: Small ribosomal subunit protein uS7m

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Macromolecule #7: 28S ribosomal protein S9, mitochondrial

MacromoleculeName: 28S ribosomal protein S9, mitochondrial / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 45.909859 KDa
SequenceString: MAAPCVSYGG AVSYRLLLWG RGSLARKQGL WKTAAPELQT NVRSQILRLR HTAFVIPKKN VPTSKRETYT EDFIKKQIEE FNIGKRHLA NMMGEDPETF TQEDIDRAIA YLFPSGLFEK RARPVMKHPE QIFPRQRAIQ WGEDGRPFHY LFYTGKQSYY S LMHDVYGM ...String:
MAAPCVSYGG AVSYRLLLWG RGSLARKQGL WKTAAPELQT NVRSQILRLR HTAFVIPKKN VPTSKRETYT EDFIKKQIEE FNIGKRHLA NMMGEDPETF TQEDIDRAIA YLFPSGLFEK RARPVMKHPE QIFPRQRAIQ WGEDGRPFHY LFYTGKQSYY S LMHDVYGM LLNLEKHQSH LQAKSLLPEK TVTRDVIGSR WLIKEELEEM LVEKLSDLDY MQFIRLLEKL LTSQCGAAEE EF VQRFRRS VTLESKKQLI EPVQYDEQGM AFSKSEGKRK TAKAEAIVYK HGSGRIKVNG IDYQLYFPIT QDREQLMFPF HFV DRLGKH DVTCTVSGGG RSAQAGAIRL AMAKALCSFV TEDEVEWMRQ AGLLTTDPRV RERKKPGQEG ARRKFTWKKR

UniProtKB: Small ribosomal subunit protein uS9m

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Macromolecule #8: 28S ribosomal protein S10, mitochondrial

MacromoleculeName: 28S ribosomal protein S10, mitochondrial / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 23.0335 KDa
SequenceString: MAARTAFGAV CRRLWQGLGN FSVNTSKGNT AKNGGLLLST NMKWVQFSNL HVDVPKDLTK PVVTISDEPD ILYKRLSVLV KGHDKAVLD SYEYFAVLAA KELGISIKVH EPPRKIERFT LLQSVHIYKK HRVQYEMRTL YRCLELEHLT GSTADVYLEY I QRNLPEGV ...String:
MAARTAFGAV CRRLWQGLGN FSVNTSKGNT AKNGGLLLST NMKWVQFSNL HVDVPKDLTK PVVTISDEPD ILYKRLSVLV KGHDKAVLD SYEYFAVLAA KELGISIKVH EPPRKIERFT LLQSVHIYKK HRVQYEMRTL YRCLELEHLT GSTADVYLEY I QRNLPEGV AMEVTKTQLE QLPEHIKEPI WETLSEEKEE SKS

UniProtKB: Small ribosomal subunit protein uS10m

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Macromolecule #9: 28S ribosomal protein S11, mitochondrial

MacromoleculeName: 28S ribosomal protein S11, mitochondrial / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 20.648793 KDa
SequenceString: MQAVRNAGSR FLRSWTWPQT AGRVVARTPA GTICTGARQL QDAAAKQKVE QNAAPSHTKF SIYPPIPGEE SSLRWAGKKF EEIPIAHIK ASHNNTQIQV VSASNEPLAF ASCGTEGFRN AKKGTGIAAQ TAGIAAAARA KQKGVIHIRV VVKGLGPGRL S AMHGLIMG ...String:
MQAVRNAGSR FLRSWTWPQT AGRVVARTPA GTICTGARQL QDAAAKQKVE QNAAPSHTKF SIYPPIPGEE SSLRWAGKKF EEIPIAHIK ASHNNTQIQV VSASNEPLAF ASCGTEGFRN AKKGTGIAAQ TAGIAAAARA KQKGVIHIRV VVKGLGPGRL S AMHGLIMG GLEVISITDN TPIPHNGCRP RKARKL

UniProtKB: Small ribosomal subunit protein uS11m

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Macromolecule #10: 28S ribosomal protein S12, mitochondrial

MacromoleculeName: 28S ribosomal protein S12, mitochondrial / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 15.200947 KDa
SequenceString:
MSWSGLLHGL NTSLTCGPAL VPRLWATCSM ATLNQMHRLG PPKRPPRKLG PTEGRPQLKG VVLCTFTRKP KKPNSANRKC CRVRLSTGR EAVCFIPGEG HTLQEHQIVL VEGGRTQDLP GVKLTVVRGK YDCGHVQKK

UniProtKB: Small ribosomal subunit protein uS12m

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Macromolecule #11: 28S ribosomal protein S14, mitochondrial

MacromoleculeName: 28S ribosomal protein S14, mitochondrial / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 15.168788 KDa
SequenceString:
MAAFMLGSLL RTFKQMVPSS ASGQVRSHYV DWRMWRDVKR RKMAYEYADE RLRINSLRKN TILPKILQDV ADEEIAALPR DSCPVRIRN RCVMTSRPRG VKRRWRLSRI VFRHLADHGQ LSGIQRATW

UniProtKB: Small ribosomal subunit protein uS14m

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Macromolecule #12: 28S ribosomal protein S15, mitochondrial

MacromoleculeName: 28S ribosomal protein S15, mitochondrial / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 29.903293 KDa
SequenceString: MLRVAWRTLS LIRTRAVTQV LVPGLPGGGS AKFPFNQWGL QPRSLLLQAA RGYVVRKPAQ SRLDDDPPPS TLLKDYQNVP GIEKVDDVV KRLLSLEMAN KKEMLKIKQE QFMKKIVANP EDTRSLEARI IALSVKIRSY EEHLEKHRKD KAHKRYLLMS I DQRKKMLK ...String:
MLRVAWRTLS LIRTRAVTQV LVPGLPGGGS AKFPFNQWGL QPRSLLLQAA RGYVVRKPAQ SRLDDDPPPS TLLKDYQNVP GIEKVDDVV KRLLSLEMAN KKEMLKIKQE QFMKKIVANP EDTRSLEARI IALSVKIRSY EEHLEKHRKD KAHKRYLLMS I DQRKKMLK NLRNTNYDVF EKICWGLGIE YTFPPLYYRR AHRRFVTKKA LCIRVFQETQ KLKKRRRALK AAAAAQKQAK RR NPDSPAK AIPKTLKDSQ

UniProtKB: Small ribosomal subunit protein uS15m

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Macromolecule #13: 28S ribosomal protein S16, mitochondrial

MacromoleculeName: 28S ribosomal protein S16, mitochondrial / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 15.371899 KDa
SequenceString:
MVHLTTLLCK AYRGGHLTIR LALGGCTNRP FYRIVAAHNK CPRDGRFVEQ LGSYDPLPNS HGEKLVALNL DRIRHWIGCG AHLSKPMEK LLGLAGFFPL HPMMITNAER LRRKRAREVL LASQKTDAEA TDTEATET

UniProtKB: Small ribosomal subunit protein bS16m

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Macromolecule #14: 28S ribosomal protein S17, mitochondrial

MacromoleculeName: 28S ribosomal protein S17, mitochondrial / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 14.526078 KDa
SequenceString:
MSVVRSSVHA RWIVGKVIGT KMQKTAKVRV TRLVLDPYLL KYFNKRKTYF AHDALQQCTV GDIVLLRALP VPRAKHVKHE LAEIVFKVG KVIDPVTGKP CAGTTYLESP LSSETTQLSK NLEELNISSA Q

UniProtKB: Small ribosomal subunit protein uS17m

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Macromolecule #15: 28S ribosomal protein S18b, mitochondrial

MacromoleculeName: 28S ribosomal protein S18b, mitochondrial / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 29.440629 KDa
SequenceString: MAASVLNTVL RRLPMLSLFR GSHRVQVPLQ TLCTKAPSEE DSLSSVPISP YKDEPWKYLE SEEYQERYGS RPVWADYRRN HKGGVPPQR TRKTCIRRNK VVGNPCPICR DHKLHVDFRN VKLLEQFVCA HTGIIFYAPY TGVCVKQHKR LTQAIQKARD H GLLIYHIP ...String:
MAASVLNTVL RRLPMLSLFR GSHRVQVPLQ TLCTKAPSEE DSLSSVPISP YKDEPWKYLE SEEYQERYGS RPVWADYRRN HKGGVPPQR TRKTCIRRNK VVGNPCPICR DHKLHVDFRN VKLLEQFVCA HTGIIFYAPY TGVCVKQHKR LTQAIQKARD H GLLIYHIP QVEPRDLDFS TSHGAVSATP PAPTLVSGDP WYPWYNWKQP PERELSRLRR LYQGHLQEES GPPPESMPKM PP RTPAEAS STGQTGPQSA L

UniProtKB: Small ribosomal subunit protein mS40

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Macromolecule #16: 28S ribosomal protein S18c, mitochondrial

MacromoleculeName: 28S ribosomal protein S18c, mitochondrial / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 15.876786 KDa
SequenceString:
MAAVVAVCGG LGRKKLTHLV TAAVSLTHPG THTVLWRRGC SQQVSSNEDL PISMENPYKE PLKKCILCGK HVDYKNVQLL SQFVSPFTG CIYGRHITGL CGKKQKEITK AIKRAQIMGF MPVTYKDPAY LKDPKVCNIR YRE

UniProtKB: Small ribosomal subunit protein bS18m

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Macromolecule #17: 28S ribosomal protein S21, mitochondrial

MacromoleculeName: 28S ribosomal protein S21, mitochondrial / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 10.675494 KDa
SequenceString:
(AYA)KHLKFIART VMVQEGNVES AYRTLNRILT MDGLIEDIKH RRYYEKPCRR RQRESYERCR RIYNMEMARK INFLMR KNR ADPWQGC

UniProtKB: Small ribosomal subunit protein bS21m

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Macromolecule #18: 28S ribosomal protein S22, mitochondrial

MacromoleculeName: 28S ribosomal protein S22, mitochondrial / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 41.337316 KDa
SequenceString: MAPLGTTVLL WSLLRSSPGV ERVCFRARIQ PWHGGLLQPL PCSFEMGLPR RRFSSEAAES GSPETKKPTF MDEEVQSILT KMTGLNLQK TFKPAIQELK PPTYKLMTQA QLEEATRQAV EAAKVRLKMP PVLEERVPIN DVLAEDKILE GTETTKYVFT D ISYSIPHR ...String:
MAPLGTTVLL WSLLRSSPGV ERVCFRARIQ PWHGGLLQPL PCSFEMGLPR RRFSSEAAES GSPETKKPTF MDEEVQSILT KMTGLNLQK TFKPAIQELK PPTYKLMTQA QLEEATRQAV EAAKVRLKMP PVLEERVPIN DVLAEDKILE GTETTKYVFT D ISYSIPHR ERFIVVREPS GTLRKASWEE RDRMIQVYFP KEGRKILTPI IFKEENLRTM YSQDRHVDVL NLCFAQFEPD ST EYIKVHH KTYEDIDKRG KYDLLRSTRY FGGMVWYFVN NKKIDGLLID QIQRDLIDDA TNLVQLYHVL HPDGQSAQGA KDQ AAEGIN LIKVFAKTEA QKGAYIELTL QTYQEALSRH SAAS

UniProtKB: Small ribosomal subunit protein mS22

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Macromolecule #19: 28S ribosomal protein S23, mitochondrial

MacromoleculeName: 28S ribosomal protein S23, mitochondrial / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 21.805664 KDa
SequenceString:
MAGSRLETVG SIFSRTRDLV RAGVLKEKPL WFDVYDAFPP LREPVFQRPR VRYGKAKAPI QDIWYHEDRI RAKFYSVYGS GQRAFDLFN PNFKSTCQRF VEKYTELQKL GETDEEKLFV ETGKALLAEG VILRRVGEAR TQHGGSHVSR KSEHLSVRPQ T ALEENETQ KEVPQDQHLE APADQSKGLL PP

UniProtKB: Small ribosomal subunit protein mS23

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Macromolecule #20: 28S ribosomal protein S25, mitochondrial

MacromoleculeName: 28S ribosomal protein S25, mitochondrial / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 20.146428 KDa
SequenceString:
MPMKGRFPIR RTLQYLSQGN VVFKDSVKVM TVNYNTHGEL GEGARKFVFF NIPQIQYKNP WVQIMMFKNM TPSPFLRFYL DSGEQVLVD VETKSNKEIM EHIRKILGKN EETLREEEEE KKQLSHPANF GPRKYCLREC ICEVEGQVPC PSLVPLPKEM R GKYKAALK ADAQD

UniProtKB: Small ribosomal subunit protein mS25

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Macromolecule #21: 28S ribosomal protein S26, mitochondrial

MacromoleculeName: 28S ribosomal protein S26, mitochondrial / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 24.25982 KDa
SequenceString: MLRALSRLGA GTPCRPRAPL VLPARGRKTR HDPLAKSKIE RVNMPPAVDP AEFFVLMERY QHYRQTVRAL RMEFVSEVQR KVHEARAGV LAERKALKDA AEHRELMAWN QAENRRLHEL RIARLRQEER EQEQRQALEQ ARKAEEVQAW AQRKEREVLQ L QEEVKNFI ...String:
MLRALSRLGA GTPCRPRAPL VLPARGRKTR HDPLAKSKIE RVNMPPAVDP AEFFVLMERY QHYRQTVRAL RMEFVSEVQR KVHEARAGV LAERKALKDA AEHRELMAWN QAENRRLHEL RIARLRQEER EQEQRQALEQ ARKAEEVQAW AQRKEREVLQ L QEEVKNFI TRENLEARVE AALDSRKNYN WAITREGLVV RPQRRDS

UniProtKB: Small ribosomal subunit protein mS26

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Macromolecule #22: 28S ribosomal protein S27, mitochondrial

MacromoleculeName: 28S ribosomal protein S27, mitochondrial / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 47.669262 KDa
SequenceString: MAASIVRRGM LLARQVVLPQ LSPAGKRYLL SSAYVDSHKW EAREKEHYCL ADLASLMDKT FERKLPVSSL TISRLIDNIS SREEIDHAE YYLYKFRHSP NCWYLRNWTI HTWIRQCLKY DAQDKALYTL VNKVQYGIFP DNFTFNLLMD SFIKKENYKD A LSVVFEVM ...String:
MAASIVRRGM LLARQVVLPQ LSPAGKRYLL SSAYVDSHKW EAREKEHYCL ADLASLMDKT FERKLPVSSL TISRLIDNIS SREEIDHAE YYLYKFRHSP NCWYLRNWTI HTWIRQCLKY DAQDKALYTL VNKVQYGIFP DNFTFNLLMD SFIKKENYKD A LSVVFEVM MQEAFEVPST QLLSLYVLFH CLAKKTDFSW EEERNFGASL LLPGLKQKNS VGFSSQLYGY ALLGKVELQQ GL RAVYHNM PLIWKPGYLD RALQVMEKVA ASPEDIKLCR EALDVLGAVL KALTSADGAS EEQSQNDEDN QGSEKLVEQL DIE ETEQSK LPQYLERFKA LHSKLQALGK IESEGLLSLT TQLVKEKLST CEAEDIATYE QNLQQWHLDL VQLIQREQQQ REQA KQEYQ AQKAAKASA

UniProtKB: Small ribosomal subunit protein mS27

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Macromolecule #23: 28S ribosomal protein S28, mitochondrial

MacromoleculeName: 28S ribosomal protein S28, mitochondrial / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 20.878846 KDa
SequenceString:
MAALCRTRAV AAESHFLRVF LFFRPFRGVG TESGSESGSS NAKEPKTRAG GFASALERHS ELLQKVEPLQ KGSPKNVESF ASMLRHSPL TQMGPAKDKL VIGRIFHIVE NDLYIDFGGK FHCVCRRPEV DGEKYQKGTR VRLRLLDLEL TSRFLGATTD T TVLEANAV LLGIQESKDS RSKEEHHEK

UniProtKB: Small ribosomal subunit protein bS1m

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Macromolecule #24: 28S ribosomal protein S29, mitochondrial

MacromoleculeName: 28S ribosomal protein S29, mitochondrial / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 45.634406 KDa
SequenceString: MMLKGITRLI SRIHKLDPGR FLHMGTQARQ SIAAHLDNQV PVESPRAISR TNENDPAKHG DQHEGQHYNI SPQDLETVFP HGLPPRFVM QVKTFSEACL MVRKPALELL HYLKNTSFAY PAIRYLLYGE KGTGKTLSLC HVIHFCAKQD WLILHIPDAH L WVKNCRDL ...String:
MMLKGITRLI SRIHKLDPGR FLHMGTQARQ SIAAHLDNQV PVESPRAISR TNENDPAKHG DQHEGQHYNI SPQDLETVFP HGLPPRFVM QVKTFSEACL MVRKPALELL HYLKNTSFAY PAIRYLLYGE KGTGKTLSLC HVIHFCAKQD WLILHIPDAH L WVKNCRDL LQSSYNKQRF DQPLEASTWL KNFKTTNERF LNQIKVQEKY VWNKRESTEK GSPLGEVVEQ GITRVRNATD AV GIVLKEL KRQSSLGMFH LLVAVDGINA LWGRTTLKRE DKSPIAPEEL ALVHNLRKMM KNDWHGGAIV SALSQTGSLF KPR KAYLPQ ELLGKEGFDA LDPFIPILVS NYNPKEFESC IQYYLENNWL QHEKAPTEEG KKELLFLSNA NPSLLERHCA YL

UniProtKB: Small ribosomal subunit protein mS29

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Macromolecule #25: 28S ribosomal protein S31, mitochondrial

MacromoleculeName: 28S ribosomal protein S31, mitochondrial / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 45.391473 KDa
SequenceString: MFPRVSTFLP LRPLSRHPLS SGSPETSAAA IMLLTVRHGT VRYRSSALLA RTKNNIQRYF GTNSVICSKK DKQSVRTEET SKETSESQD SEKENTKKDL LGIIKGMKVE LSTVNVRTTK PPKRRPLKSL EATLGRLRRA TEYAPKKRIE PLSPELVAAA S AVADSLPF ...String:
MFPRVSTFLP LRPLSRHPLS SGSPETSAAA IMLLTVRHGT VRYRSSALLA RTKNNIQRYF GTNSVICSKK DKQSVRTEET SKETSESQD SEKENTKKDL LGIIKGMKVE LSTVNVRTTK PPKRRPLKSL EATLGRLRRA TEYAPKKRIE PLSPELVAAA S AVADSLPF DKQTTKSELL SQLQQHEEES RAQRDAKRPK ISFSNIISDM KVARSATARV RSRPELRIQF DEGYDNYPGQ EK TDDLKKR KNIFTGKRLN IFDMMAVTKE APETDTSPSL WDVEFAKQLA TVNEQPLQNG FEELIQWTKE GKLWEFPINN EAG FDDDGS EFHEHIFLEK HLESFPKQGP IRHFMELVTC GLSKNPYLSV KQKVEHIEWF RNYFNEKKDI LKESNIQFN

UniProtKB: Small ribosomal subunit protein mS31

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Macromolecule #26: 28S ribosomal protein S33, mitochondrial

MacromoleculeName: 28S ribosomal protein S33, mitochondrial / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 12.657776 KDa
SequenceString:
MSSLSEYAFR MSRLSARLFG EVTRPTNSKS MKVVKLFSEL PLAKKKETYD WYPNHHTYAE LMQTLRFLGL YRDEHQDFMD EQKRLKKLR GKEKPKKGEG KRAAKRK

UniProtKB: Small ribosomal subunit protein mS33

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Macromolecule #27: 28S ribosomal protein S34, mitochondrial

MacromoleculeName: 28S ribosomal protein S34, mitochondrial / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 25.695549 KDa
SequenceString: MARKKVRPRL IAELARRVRA LREQLNRPRD SQLYAVDYET LTRPFSGRRL PVRAWADVRR ESRLLQLLGR LPLFGLGRLV TRKSWLWQH DEPCYWRLTR VRPDYTAQNL DHGKAWGILT FKGKTESEAR EIEHVMYHDW RLVPKHEEEA FTAFTPAPED S LASVPYPP ...String:
MARKKVRPRL IAELARRVRA LREQLNRPRD SQLYAVDYET LTRPFSGRRL PVRAWADVRR ESRLLQLLGR LPLFGLGRLV TRKSWLWQH DEPCYWRLTR VRPDYTAQNL DHGKAWGILT FKGKTESEAR EIEHVMYHDW RLVPKHEEEA FTAFTPAPED S LASVPYPP LLRAMIIAER QKNGDTSTEE PMLNVQRIRM EPWDYPAKQE DKGRAKGTPV

UniProtKB: Small ribosomal subunit protein mS34

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Macromolecule #28: 28S ribosomal protein S35, mitochondrial

MacromoleculeName: 28S ribosomal protein S35, mitochondrial / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 36.898328 KDa
SequenceString: MAAAALPAWL SLQSRARTLR AFSTAVYSAT PVPTPSLPER TPGNERPPRR KALPPRTEKM AVDQDWPSVY PVAAPFKPSA VPLPVRMGY PVKKGVPMAK EGNLELLKIP NFLHLTPVAI KKHCEALKDF CTEWPAALDS DEKCEKHFPI EIDSTDYVSS G PSVRNPRA ...String:
MAAAALPAWL SLQSRARTLR AFSTAVYSAT PVPTPSLPER TPGNERPPRR KALPPRTEKM AVDQDWPSVY PVAAPFKPSA VPLPVRMGY PVKKGVPMAK EGNLELLKIP NFLHLTPVAI KKHCEALKDF CTEWPAALDS DEKCEKHFPI EIDSTDYVSS G PSVRNPRA RVVVLRVKLS SLNLDDHAKK KLIKLVGERY CKTTDVLTIK TDRCPLRRQN YDYAVYLLTV LYHESWNTEE WE KSKTEAD MEEYIWENSS SERNILETLL QMKAAEKNME INKEELLGTK EIEEYKKSVV SLKNEEENEN SISQYKESVK RLL NVT

UniProtKB: Small ribosomal subunit protein mS35

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Macromolecule #29: Coiled-coil-helix-coiled-coil-helix domain-containing protein 1

MacromoleculeName: Coiled-coil-helix-coiled-coil-helix domain-containing protein 1
type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 13.409661 KDa
SequenceString:
(AYA)TPSLRGRLA RFGNPRKPVL KPNKPLILAN RVGERRREKG EATCITEMSV MMACWKQNEF RDDACRKEIQ GFLDCA ARA QEARKMRSIQ ETLGESGSLL PNKLNKLLQR FPNKPYLS

UniProtKB: Small ribosomal subunit protein mS37

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Macromolecule #30: Aurora kinase A-interacting protein

MacromoleculeName: Aurora kinase A-interacting protein / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 22.395326 KDa
SequenceString: MLLGRLTSQL LRAVPWAGGR PPWPVSGVLG SRVCGPLYST SPAGPGRAAS LPRKGAQLEL EEMLVPRKMS VSPLESWLTA RCFLPRLDT GTAGTVAPPQ SYQCPPSQIG EGAEQGDEGV ADAPQIQCKN VLKIRRRKMN HHKYRKLVKK TRFLRRKVQE G RLRRKQIK ...String:
MLLGRLTSQL LRAVPWAGGR PPWPVSGVLG SRVCGPLYST SPAGPGRAAS LPRKGAQLEL EEMLVPRKMS VSPLESWLTA RCFLPRLDT GTAGTVAPPQ SYQCPPSQIG EGAEQGDEGV ADAPQIQCKN VLKIRRRKMN HHKYRKLVKK TRFLRRKVQE G RLRRKQIK FEKDLRRIWL KAGLKEAPEG WQTPKIYLRG K

UniProtKB: Small ribosomal subunit protein mS38

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Macromolecule #31: Pentatricopeptide repeat domain-containing protein 3, mitochondrial

MacromoleculeName: Pentatricopeptide repeat domain-containing protein 3, mitochondrial
type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 78.648547 KDa
SequenceString: MAVVSAVRWL GLRSRLGQPL TGRRAGLCEQ ARSCRFYSGS ATLSKVEGTD VTGIEEVVIP KKKTWDKVAV LQALASTVNR DTTAVPYVF QDDPYLMPAS SLESRSFLLA KKSGENVAKF IINSYPKYFQ KDIAEPHIPC LMPEYFEPQI KDISEAALKE R IELRKVKA ...String:
MAVVSAVRWL GLRSRLGQPL TGRRAGLCEQ ARSCRFYSGS ATLSKVEGTD VTGIEEVVIP KKKTWDKVAV LQALASTVNR DTTAVPYVF QDDPYLMPAS SLESRSFLLA KKSGENVAKF IINSYPKYFQ KDIAEPHIPC LMPEYFEPQI KDISEAALKE R IELRKVKA SVDMFDQLLQ AGTTVSLETT NSLLDLLCYY GDQEPSTDYH FQQTGQSEAL EEENDETSRR KAGHQFGVTW RA KNNAERI FSLMPEKNEH SYCTMIRGMV KHRAYEQALN LYTELLNNRL HADVYTFNAL IEATVCAINE KFEEKWSKIL ELL RHMVAQ KVKPNLQTFN TILKCLRRFH VFARSPALQV LREMKAIGIE PSLATYHHII RLFDQPGDPL KRSSFIIYDI MNEL MGKRF SPKDPDDDKF FQSAMSICSS LRDLELAYQV HGLLKTGDNW KFIGPDQHRN FYYSKFFDLI CLMEQIDVTL KWYED LIPS AYFPHSQTMI HLLQALDVAN RLEVIPKIWK DSKEYGHTFR SDLREEILML MARDKHPPEL QVAFADCAAD IKSAYE SQP IRQTAQDWPA TSLNCIAILF LRAGRTQEAW KMLGLFRKHN KIPRSELLNE LMDSAKVSNS PSQAIEVVEL ASAFSLP IC EGLTQRVMSD FAINQEQKEA LSNLTALTSD SDTDSSSDSD SDTSEGK

UniProtKB: Small ribosomal subunit protein mS39

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Macromolecule #32: Translation initiation factor IF-2, mitochondrial

MacromoleculeName: Translation initiation factor IF-2, mitochondrial / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 77.944203 KDa
Recombinant expressionOrganism: Escherichia coli BL21(DE3) (bacteria)
SequenceString: MSSAYPVWTA QLCAWPWPTD VLTGAALSQY RLLVTKKEEG PWKSQLSSTK SKKVVEVWIG MTIEELARAM EKNTDYVYEA LLNTDIDID SLEADSHLDE VWIKEVITKA GMKLKWSKLK QDKVRKNKDA VRRPQADPAL LTPRSPVVTI MGHVDHGKTT L LDKFRKTQ ...String:
MSSAYPVWTA QLCAWPWPTD VLTGAALSQY RLLVTKKEEG PWKSQLSSTK SKKVVEVWIG MTIEELARAM EKNTDYVYEA LLNTDIDID SLEADSHLDE VWIKEVITKA GMKLKWSKLK QDKVRKNKDA VRRPQADPAL LTPRSPVVTI MGHVDHGKTT L LDKFRKTQ VAAVETGGIT QHIGAFLVSL PSGEKITFLD TPGHAAFSAM RARGAQVTDI VVLVVAADDG VMKQTVESIQ HA KDAQVPI ILAVNKCDKA EADPEKVKKE LLAYDVVCED YGGDVQAVPV SALTGDNLMA LAEATVALAE MLELKADPNG PVE GTVIES FTDKGRGLVT TAIIQRGTLR KGSVLVAGKC WAKVRLMFDE NGKTIDEAYP SMPVGITGWR DLPSAGEEIL EVES EPRAR EVVDWRKYEQ EQEKGQEDLK IIEEKRKEHK EAHQKAREKY GHLLWKKRSI LRFLERKEQI PLKPKEKRER DSNVL SVII KGDVDGSVEA ILNIIDTYDA SHECELELVH FGVGDVSAND VNLAETFDGV IYGFNVNAGN VIQQSAAKKG VKIKLH KII YRLVEDLQEE LSSRLPCAVE EHPVGEASIL ATFSVTEGKK KVPVAGCRVQ KGQLEKQKKF KLTRNGHVIW KGSLTSL KH HKDDISIVKT GMDCGLSLDE DNMEFQVGDR IVCYEEKQIQ AKTSWDPGFL EHHHHHH

UniProtKB: Translation initiation factor IF-2, mitochondrial

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Macromolecule #33: Translation initiation factor IF-3, mitochondrial

MacromoleculeName: Translation initiation factor IF-3, mitochondrial / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 32.545461 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MAALFLKRLT LQTVKSENSC IRCFGKHILQ KTAPAQLSPI ASAPRLSFLI HAKAFSTAED TQNEGKKIKK NKTAFSNVGR KISQRVIHL FDEKGNDLGN MHRANVIRLM DERDLRLVQR NTSTEPAEYQ LMTGLQILQE RQRLREMEKA NPKTGPTLRK E LILSSNIG ...String:
MAALFLKRLT LQTVKSENSC IRCFGKHILQ KTAPAQLSPI ASAPRLSFLI HAKAFSTAED TQNEGKKIKK NKTAFSNVGR KISQRVIHL FDEKGNDLGN MHRANVIRLM DERDLRLVQR NTSTEPAEYQ LMTGLQILQE RQRLREMEKA NPKTGPTLRK E LILSSNIG QHDLDTKTKQ IQQWIKKKHL VQITIKKGKN VDVSENEMEE IFHQILQTMP GIATFSSRPQ AVQGGKALMC VL RALSKNE EKAYKETQET QERDTLNKDH GNDKESNVLH QGLEVLFQ

UniProtKB: Translation initiation factor IF-3, mitochondrial

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Macromolecule #34: NICOTINAMIDE-ADENINE-DINUCLEOTIDE

MacromoleculeName: NICOTINAMIDE-ADENINE-DINUCLEOTIDE / type: ligand / ID: 34 / Number of copies: 1 / Formula: NAD
Molecular weightTheoretical: 663.425 Da
Chemical component information

ChemComp-NAD:
NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NAD*YM

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Macromolecule #35: SPERMINE

MacromoleculeName: SPERMINE / type: ligand / ID: 35 / Number of copies: 1 / Formula: SPM
Molecular weightTheoretical: 202.34 Da
Chemical component information

ChemComp-SPM:
SPERMINE

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Macromolecule #36: STREPTOMYCIN

MacromoleculeName: STREPTOMYCIN / type: ligand / ID: 36 / Number of copies: 1 / Formula: SRY
Molecular weightTheoretical: 581.574 Da
Chemical component information

ChemComp-SRY:
STREPTOMYCIN / medication, antibiotic*YM

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Macromolecule #37: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 37 / Number of copies: 61 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #38: POTASSIUM ION

MacromoleculeName: POTASSIUM ION / type: ligand / ID: 38 / Number of copies: 17 / Formula: K
Molecular weightTheoretical: 39.098 Da

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Macromolecule #39: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 39 / Number of copies: 1 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #40: FE2/S2 (INORGANIC) CLUSTER

MacromoleculeName: FE2/S2 (INORGANIC) CLUSTER / type: ligand / ID: 40 / Number of copies: 2 / Formula: FES
Molecular weightTheoretical: 175.82 Da
Chemical component information

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER

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Macromolecule #41: ADENOSINE-5'-TRIPHOSPHATE

MacromoleculeName: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 41 / Number of copies: 1 / Formula: ATP
Molecular weightTheoretical: 507.181 Da
Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

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Macromolecule #42: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER

MacromoleculeName: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / type: ligand / ID: 42 / Number of copies: 2 / Formula: GNP
Molecular weightTheoretical: 522.196 Da
Chemical component information

ChemComp-GNP:
PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / GppNHp, GMPPNP, energy-carrying molecule analogue*YM

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Macromolecule #43: water

MacromoleculeName: water / type: ligand / ID: 43 / Number of copies: 61 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.7 mg/mL
BufferpH: 7.5
Component:
ConcentrationFormulaName
0.1 MKClPotassium chloride
25.0 mMHEPES-KOH4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid-potassium hydroxide buffer
5.0 mMMg(CH3COO)2Magnesium acetate
0.25 mMC10H17N6O13P35'-Guanylyl imidodiphosphate
0.05 %C24H46O11n-Dodecyl-beta-D-maltopyranoside
GridModel: Quantifoil R2/2 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 40 sec. / Details: Grid was coated with 3 nm carbon film
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Specialist opticsEnergy filter - Name: GIF Quantum LS / Energy filter - Slit width: 20 eV
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Digitization - Dimensions - Width: 3838 pixel / Digitization - Dimensions - Height: 3710 pixel / Digitization - Frames/image: 2-20 / Number grids imaged: 1 / Number real images: 13831 / Average exposure time: 4.0 sec. / Average electron dose: 1.5 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 70.0 µm / Calibrated defocus max: 5.0 µm / Calibrated defocus min: 0.25 µm / Calibrated magnification: 165000 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 3.2 µm / Nominal defocus min: 1.0 µm / Nominal magnification: 130000
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 1103314
Startup modelType of model: EMDB MAP
EMDB ID:

2880

Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.14 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.0.2) / Number images used: 103165
Initial angle assignmentType: PROJECTION MATCHING / Software - Name: RELION (ver. 2.1.0)
Final angle assignmentType: ANGULAR RECONSTITUTION / Software - Name: RELION (ver. 2.1.0)
Final 3D classificationSoftware - Name: RELION (ver. 3.0.2)
FSC plot (resolution estimation)

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Atomic model buiding 1

Initial modelPDB ID:

3j9m


Chain - Source name: PDB / Chain - Initial model type: experimental model
RefinementSpace: REAL / Protocol: AB INITIO MODEL / Overall B value: 42 / Target criteria: Cross-correlation coefficient
Output model

PDB-6rw5:
Structure of human mitochondrial 28S ribosome in complex with mitochondrial IF2 and IF3

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