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- EMDB-9358: Structure of human mitochondrial translation initiation factor 3 ... -

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Basic information

Entry
Database: EMDB / ID: EMD-9358
TitleStructure of human mitochondrial translation initiation factor 3 bound to the small ribosomal subunit-Class-II
Map dataStructure of human mitochondrial translation initiation factor 3 bound to the smallribosomal subunit
Sample
  • Complex: Structure of human mitochondrial translation initiation factor 3 bound to the small ribosomal subunit
    • RNA: x 1 types
    • Protein or peptide: x 31 types
Function / homology
Function and homology information


Mitochondrial translation elongation / Mitochondrial translation termination / mitochondrial translational initiation / peptide biosynthetic process / mitochondrial ribosome binding / translation factor activity, RNA binding / ribosome disassembly / mitochondrial ribosome assembly / positive regulation of mitochondrial translation / Mitochondrial translation initiation ...Mitochondrial translation elongation / Mitochondrial translation termination / mitochondrial translational initiation / peptide biosynthetic process / mitochondrial ribosome binding / translation factor activity, RNA binding / ribosome disassembly / mitochondrial ribosome assembly / positive regulation of mitochondrial translation / Mitochondrial translation initiation / mitochondrial small ribosomal subunit / mitochondrial ribosome / mitochondrial translation / ribosomal small subunit binding / translation initiation factor activity / small ribosomal subunit rRNA binding / ribosomal small subunit assembly / ribosome binding / regulation of translation / small ribosomal subunit / kinase activity / mitochondrial inner membrane / cell population proliferation / tRNA binding / rRNA binding / ribosome / structural constituent of ribosome / translation / ribonucleoprotein complex / protein domain specific binding / phosphorylation / mRNA binding / apoptotic process / nucleolus / mitochondrion / RNA binding / nucleoplasm
Similarity search - Function
Translation initiation factor 3 / Translation initiation factor 3, N-terminal / Translation initiation factor 3 (IF-3), N-terminal domain superfamily / Translation initiation factor 3 (IF-3), C-terminal domain superfamily / Translation initiation factor IF-3, N-terminal domain / Coiled-coil-helix-coiled-coil-helix domain-containing protein 1 / 28S ribosomal protein S26 / Mitochondrial ribosome subunit S26 / Cysteine alpha-hairpin motif superfamily / 28S ribosomal protein S27, mitochondrial ...Translation initiation factor 3 / Translation initiation factor 3, N-terminal / Translation initiation factor 3 (IF-3), N-terminal domain superfamily / Translation initiation factor 3 (IF-3), C-terminal domain superfamily / Translation initiation factor IF-3, N-terminal domain / Coiled-coil-helix-coiled-coil-helix domain-containing protein 1 / 28S ribosomal protein S26 / Mitochondrial ribosome subunit S26 / Cysteine alpha-hairpin motif superfamily / 28S ribosomal protein S27, mitochondrial / MRPS27/PTCD2 / Mitochondrial 28S ribosomal protein S27 / Ribosomal protein S22, mitochondrial / Ribosomal protein S23, mitochondrial / Ribosomal protein S23/S25, mitochondrial / Mitochondrial 28S ribosomal protein S31 / Mitochondrial 28S ribosomal protein S22 / Mitochondrial ribosomal protein S23 / Mitochondrial 28S ribosomal protein S31 / Ribosomal protein S29, mitochondrial / Ribosomal protein S28, mitochondrial / 28S ribosomal protein S10, mitochondrial / Mitochondrial ribosomal protein MRP-S35 / 28S ribosomal protein S24, mitochondrial / Mitochondrial 28S ribosomal protein S34 / Pentatricopeptide repeat domain-containing protein 3 / 28S ribosomal protein S17, mitochondrial / 28S ribosomal protein S18b, mitochondrial / : / Mitochondrial ribosome subunit S24 / Mitochondrial 28S ribosomal protein S34 / 40S ribosomal protein rpS2 (S5p), N-terminal / 28S ribosomal protein S25, mitochondrial / Pentatricopeptide repeat domain / Ribosomal protein S27/S33, mitochondrial / Ribosomal protein S24/S35, mitochondrial / Mitochondrial ribosomal subunit S27 / Ribosomal protein S24/S35, mitochondrial, conserved domain / Mitochondrial ribosomal subunit protein / Pentatricopeptide (PPR) repeat profile. / Ribosomal protein S23/S29, mitochondrial / Mitochondrial ribosomal death-associated protein 3 / Pentatricopeptide repeat / Mitochondrial mRNA-processing protein COX24, C-terminal / Mitochondrial mRNA-processing protein COX24, C-terminal / Mitochondrial domain of unknown function (DUF1713) / CHCH / CHCH domain / Coiled coil-helix-coiled coil-helix (CHCH) domain profile. / Ribosomal protein/NADH dehydrogenase domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / Ribosomal protein S21 superfamily / Ribosomal protein S21 / Ribosomal protein S21 / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal protein S18, conserved site / Ribosomal protein S18 signature. / Ribosomal protein S16 / Ribosomal protein S16 / Ribosomal protein S16 domain superfamily / Ribosomal protein S15, bacterial-type / Ribosomal protein S6 / Ribosomal protein S6 / Ribosomal protein S6 superfamily / Ribosomal protein S12, bacterial-type / Translation elongation factor EF1B/ribosomal protein S6 / Ribosomal protein S18 / Ribosomal protein S18 / Ribosomal protein S18 superfamily / Ribosomal protein S2 signature 1. / Ribosomal protein S2, conserved site / Ribosomal protein S2 / Ribosomal protein S2, flavodoxin-like domain superfamily / Ribosomal protein S2 / Ribosomal protein S14 / Ribosomal protein S14p/S29e / Ribosomal protein S5, N-terminal, conserved site / Ribosomal protein S5 signature. / Ribosomal protein S5 / Ribosomal protein S5, N-terminal / Ribosomal protein S10p/S20e / S5 double stranded RNA-binding domain profile. / Ribosomal protein S5, C-terminal / Ribosomal protein S9, conserved site / Ribosomal protein S5, N-terminal domain / Ribosomal protein S10 domain / Ribosomal protein S10 domain superfamily / Ribosomal protein S5, C-terminal domain / Ribosomal protein S5/S7 / Ribosomal protein S7 domain / Ribosomal protein S7 domain superfamily / Ribosomal protein S10p/S20e / Ribosomal protein S9 / Ribosomal S11, conserved site / Ribosomal protein S17/S11 / Ribosomal_S15 / Ribosomal protein S15 / Ribosomal protein S12/S23 / Ribosomal protein S7p/S5e
Similarity search - Domain/homology
DAP3 protein / Small ribosomal subunit protein mS22 / Small ribosomal subunit protein mS25 / Small ribosomal subunit protein uS10m / Small ribosomal subunit protein uS11m / Small ribosomal subunit protein uS15m / Small ribosomal subunit protein bS16m / Small ribosomal subunit protein uS17m / Small ribosomal subunit protein bS18m / Small ribosomal subunit protein mS40 ...DAP3 protein / Small ribosomal subunit protein mS22 / Small ribosomal subunit protein mS25 / Small ribosomal subunit protein uS10m / Small ribosomal subunit protein uS11m / Small ribosomal subunit protein uS15m / Small ribosomal subunit protein bS16m / Small ribosomal subunit protein uS17m / Small ribosomal subunit protein bS18m / Small ribosomal subunit protein mS40 / Small ribosomal subunit protein bS21m / Small ribosomal subunit protein uS2m / Small ribosomal subunit protein mS31 / Small ribosomal subunit protein mS33 / Small ribosomal subunit protein bS1m / Small ribosomal subunit protein mS34 / Small ribosomal subunit protein bS6m / Small ribosomal subunit protein mS38 / Small ribosomal subunit protein uS12m / Coiled-coil-helix-coiled-coil-helix domain containing 1 / Small ribosomal subunit protein mS39 / Small ribosomal subunit protein uS5m / Small ribosomal subunit protein uS3m / Small ribosomal subunit protein mS23 / Small ribosomal subunit protein mS35 / Small ribosomal subunit protein mS27 / Small ribosomal subunit protein mS26 / Small ribosomal subunit protein uS7m / Small ribosomal subunit protein uS9m / Small ribosomal subunit protein uS14m / Translation initiation factor IF-3, mitochondrial
Similarity search - Component
Biological speciesHomo sapiens (human) / Bovine (cattle) / Bos taurus (cattle) / Human (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.32 Å
AuthorsSharma M / Koripella R / Agrawal R
Funding support United States, 2 items
OrganizationGrant numberCountry
National Institutes of Health/Eunice Kennedy Shriver National Institute of Child Health & Human Development (NIH/NICHD)R01 GM61576 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R01 GM 32734 United States
CitationJournal: iScience / Year: 2019
Title: Structure of Human Mitochondrial Translation Initiation Factor 3 Bound to the Small Ribosomal Subunit.
Authors: Ravi K Koripella / Manjuli R Sharma / Md Emdadul Haque / Paul Risteff / Linda L Spremulli / Rajendra K Agrawal /
Abstract: The human mitochondrial translational initiation factor 3 (IF3) carries mitochondrial-specific amino acid extensions at both its N and C termini (N- and C-terminal extensions [NTE and CTE, ...The human mitochondrial translational initiation factor 3 (IF3) carries mitochondrial-specific amino acid extensions at both its N and C termini (N- and C-terminal extensions [NTE and CTE, respectively]), when compared with its eubacterial counterpart. Here we present 3.3- to 3.5-Å-resolution cryoelectron microscopic structures of the mammalian 28S mitoribosomal subunit in complex with human IF3. Unique contacts observed between the 28S subunit and N-terminal domain of IF3 explain its unusually high affinity for the 28S subunit, whereas the position of the mito-specific NTE suggests NTE's role in binding of initiator tRNA to the 28S subunit. The location of the C-terminal domain (CTD) clarifies its anti-association activity, whereas the orientation of the mito-specific CTE provides a mechanistic explanation for its role in destabilizing initiator tRNA in the absence of mRNA. Furthermore, our structure hints at a possible role of the CTD in recruiting leaderless mRNAs for translation initiation. Our findings highlight unique features of IF3 in mitochondrial translation initiation.
History
DepositionDec 18, 2018-
Header (metadata) releaseFeb 27, 2019-
Map releaseFeb 27, 2019-
UpdateDec 11, 2019-
Current statusDec 11, 2019Processing site: RCSB / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.3
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.3
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6neq
  • Surface level: 0.3
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_9358.png

Downloads & links

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Map

FileDownload / File: emd_9358.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationStructure of human mitochondrial translation initiation factor 3 bound to the smallribosomal subunit
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.07 Å/pix.
x 400 pix.
= 429.3 Å		1.07 Å/pix.
x 400 pix.
= 429.3 Å		1.07 Å/pix.
x 400 pix.
= 429.3 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.07325 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.2 / Movie #1: 0.3
Minimum - Maximum-0.8960064 - 2.475344
Average (Standard dev.)-0.0011143155 (±0.07684857)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions400400400
Spacing400400400
CellA=B=C: 429.30002 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.073251.073251.07325
M x/y/z400400400
origin x/y/z0.0000.0000.000
length x/y/z429.300429.300429.300
α/β/γ90.00090.00090.000
start NX/NY/NZ000
NX/NY/NZ364364364
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS400400400
D min/max/mean-0.8962.475-0.001

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Supplemental data

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Sample components

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Entire : Structure of human mitochondrial translation initiation factor 3 ...

EntireName: Structure of human mitochondrial translation initiation factor 3 bound to the small ribosomal subunit
Components
  • Complex: Structure of human mitochondrial translation initiation factor 3 bound to the small ribosomal subunit
    • RNA: 28S ribosomal RNA, mitochondrial
    • Protein or peptide: 28S ribosomal protein S24, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S5, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S12, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S16, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S17, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S22, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S25, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S26, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S27, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S34, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S18b, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S7, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S9, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S10, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S14, mitochondrialRibosome
    • Protein or peptide: DAP3 protein
    • Protein or peptide: 28S ribosomal protein S31, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S33, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S35, mitochondrialRibosome
    • Protein or peptide: Coiled-coil-helix-coiled-coil-helix domain containing 1
    • Protein or peptide: Pentatricopeptide repeat domain-containing protein 3, mitochondrial
    • Protein or peptide: 28S ribosomal protein S2, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S6, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S11, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S15, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S18c, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S21, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S23, mitochondrialRibosome
    • Protein or peptide: 28S ribosomal protein S28, mitochondrialRibosome
    • Protein or peptide: Aurora kinase A interacting protein 1
    • Protein or peptide: Translation initiation factor IF-3, mitochondrial

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Supramolecule #1: Structure of human mitochondrial translation initiation factor 3 ...

SupramoleculeName: Structure of human mitochondrial translation initiation factor 3 bound to the small ribosomal subunit
type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Details: Structure of human mitochondrial translation initiation factor 3 bound to the small ribosomal subunit
Source (natural)Organism: Homo sapiens (human)

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Macromolecule #1: 28S ribosomal RNA, mitochondrial

MacromoleculeName: 28S ribosomal RNA, mitochondrial / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Bovine (cattle)
Molecular weightTheoretical: 306.932719 KDa
SequenceString: CAUAGGUUUG GUCCCAGCCU UCCUGUUAAC UCUUAAUAAA CUUACACAUG CAAGCAUCUA CACCCCAGUG AGAAUGCCCU CUAGGUUAU UAAAACUAAG AGGAGCUGGC AUCAAGCACA CACCCUGUAG CUCACGACGC CUUGCUUAAC CACACCCCAC G GGAAACAG ...String:
CAUAGGUUUG GUCCCAGCCU UCCUGUUAAC UCUUAAUAAA CUUACACAUG CAAGCAUCUA CACCCCAGUG AGAAUGCCCU CUAGGUUAU UAAAACUAAG AGGAGCUGGC AUCAAGCACA CACCCUGUAG CUCACGACGC CUUGCUUAAC CACACCCCAC G GGAAACAG CAGUGACAAA AAUUAAGCCA UAAACGAAAG UUUGACUAAG UUAUAUUAAU UAGGGUUGGU AAAUCUCGUG CC AGCCACC GCGGUCAUAC GAUUAACCCA AGCUAACAGG AGUACGGCGU AAAACGUGUU AAAGCACCAU ACCAAAUAGG GUU AAAUUC UAACUAAGCU GUAAAAAGCC AUGAUUAAAA UAAAAAUAAA UGACGAAAGU GACCCUACAA UAGCCGACGC ACUA UAGCU AAGACCCAAA CUGGGAUUAG AUACCCCACU AUGCUUAGCC CUAAACACAG AUAAUUACAU AAACAAAAUU AUUCG CCAG AGUACUACUA GCAACAGCUU AAAACUCAAA GGACUUGGCG GUGCUUUAUA UCCUUCUAGA GGAGCCUGUU CUAUAA UCG AUAAACCCCG AUAAACCUCA CCAAUUCUUG CUAAUACAGU CUAUAUACCG CCAUCUUCAG CAAACCCUAA AAAGGAA AA AAAGUAAGCG UAAUUAUGAU ACAUAAAAAC GUUAGGUCAA GGUGUAACCU AUGAAAUGGG AAGAAAUGGG CUACAUUC U CUACACCAAG AGAAUCAAGC ACGAAAGUUA UUAUGAAACC AAUAACCAAA GGAGGAUUUA GCAGUAAACU AAGAAUAGA GUGCUUAGUU GAAUUAGGCC AUGAAGCACG CACACACCGC CCGUCACCCU CCUCAAAUAG AUUCAGUGCA UCUAACCCUA UUUAAACGC ACUAGCUACA UGAGAGGAGA CAAGUCGUAA CAAGGUAAGC AUACUGGAAA GUGUGCUUGG AUAAAU

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Macromolecule #2: 28S ribosomal protein S24, mitochondrial

MacromoleculeName: 28S ribosomal protein S24, mitochondrial / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bovine (cattle)
Molecular weightTheoretical: 19.111455 KDa
SequenceString:
MVALYCGGGL RPLMLSWSRD LPCIWRALHT SAVCFKNRAA RVRVGKGNKP VTYEEAHAPH YIAHRKGWLS LHTGNLDGED HAAERTVED VFLRKFMLGT FPGCLADQLI LKRRANQVEI CALVLRQLPA HKFYFLVGYS ETLLSHFYKC PVRLHLQTVP S KVVYKYI

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Macromolecule #3: 28S ribosomal protein S5, mitochondrial

MacromoleculeName: 28S ribosomal protein S5, mitochondrial / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bovine (cattle)
Molecular weightTheoretical: 47.956941 KDa
SequenceString: MAAAVRAAGF LPALCGASAG RLWSRQLYLN TFPTASIWAL KAVPSNGPSS SAGARGRCRS THLGPALQTQ CCTPAPGNVT AQQYRSYSF FTKLTADELW KGALAETGAG ARKGRGKRTK RKRRKDLNRG QIIGEGRRGF LWPGLNAPLM KSGAIQTITQ R SKEEQEKV ...String:
MAAAVRAAGF LPALCGASAG RLWSRQLYLN TFPTASIWAL KAVPSNGPSS SAGARGRCRS THLGPALQTQ CCTPAPGNVT AQQYRSYSF FTKLTADELW KGALAETGAG ARKGRGKRTK RKRRKDLNRG QIIGEGRRGF LWPGLNAPLM KSGAIQTITQ R SKEEQEKV EADMVQQREE WDRKRKMKVK RERGWSGNSW GGISLGPPDP GPNGETYDDF DTRILEVRNV FNMTAKEGRK RS VRVLVAV GNGRGAAGFA IGKATERADA FRKAKNRAVH YLHYIERYED HTIYHDISLT FKRTHIKMKK QPRGYGLRCH RAI TTICRL IGIKDMYAKV SGSVNMLSLT RGLFQGLSRQ ETHQQLADKK SLHVVEFREE CGPLPIVVAS PQGALRKDPE PEDE VPDIK LDWDDVKAVQ GMKRSVWSGL KRAAT

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Macromolecule #4: 28S ribosomal protein S12, mitochondrial

MacromoleculeName: 28S ribosomal protein S12, mitochondrial / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bovine (cattle)
Molecular weightTheoretical: 15.479285 KDa
SequenceString:
MSWSGLLRGL SMSLNYGLAL APRPWGTRPM ATLNQLHRRG PPKFPPSKPG PTEGRPQLKG VVLRTFIRKP KKPNSANRKC CRVRLSTGR EAVCFIPGEG HNLQEHHVVL VQGGRTQDLP GVKLKVVRGK YDCGHVQKKK

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Macromolecule #5: 28S ribosomal protein S16, mitochondrial

MacromoleculeName: 28S ribosomal protein S16, mitochondrial / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bovine (cattle)
Molecular weightTheoretical: 15.157631 KDa
SequenceString:
MVQLTTVLCK AYRGGHLTIR LALGGCTNRP FYRIVAAHNK CPRDGRFVEQ LGSYDPMPNS HGEKLVALNL DRIRHWIGCG AHLSKPVEK LLGLSGFFPL HPMVITNAER LRRKRAQEVL LAAQKTDTEA TETKEN

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Macromolecule #6: 28S ribosomal protein S17, mitochondrial

MacromoleculeName: 28S ribosomal protein S17, mitochondrial / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bovine (cattle)
Molecular weightTheoretical: 14.497051 KDa
SequenceString:
MSVVRSSVHA KWIVGKVIGT AMQKTAKVRV TRLVLDPYLL KYFNKRKTYF AHDALQQCTV GDIVLLKALP VPRTKHVKHE LAEIVFKVG QVVDPVTGKR CAGTTYLESP VDLETTPLAK NLEELSLSTT Q

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Macromolecule #7: 28S ribosomal protein S22, mitochondrial

MacromoleculeName: 28S ribosomal protein S22, mitochondrial / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bovine (cattle)
Molecular weightTheoretical: 40.720562 KDa
SequenceString: MATLRVSLSL WNLHAGSRGA GRVYFRARAR PRPGDLFQPL PGVCGAGTPC RGLCSEAESG SPKIKKPTFM DEEVQSILIK MTGLDLLKI FKPAVQETKP PTYKLMTQAQ LEEATRQAIE AAKVRLKMPP VLEERTPIND VLAEDKILEG TETGKYVFTD I SYSIPHRE ...String:
MATLRVSLSL WNLHAGSRGA GRVYFRARAR PRPGDLFQPL PGVCGAGTPC RGLCSEAESG SPKIKKPTFM DEEVQSILIK MTGLDLLKI FKPAVQETKP PTYKLMTQAQ LEEATRQAIE AAKVRLKMPP VLEERTPIND VLAEDKILEG TETGKYVFTD I SYSIPHRE RFIVVREPSG TLRKASWEER DRMIQIYFPK EGRRVLTPVI FREENLQTMY SQDRHVDVLN LCVAQFEPDS AD YIKVHHQ TYEDIDKYGK YDLLRSTRHF GGMAWYFVNK KKIDGLLIDQ IQRDLVDDAA SLVQLYHILH PDGQSAQEAK EQA AEGLQL IKVFAKTEAQ KGAYIELTLQ AYQEAFISSS AAS

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Macromolecule #8: 28S ribosomal protein S25, mitochondrial

MacromoleculeName: 28S ribosomal protein S25, mitochondrial / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bovine (cattle)
Molecular weightTheoretical: 20.085312 KDa
SequenceString:
MPMKGRFPIR RTLQYLSQGD VVFKDSVKVM TVNYNTHGEL GEGARKFVFF NIPQIQYKNP WVQIMLFKNM TPTPFLRFYL DSGEQVLVD VETKSNKEIM EHVKKILGKN EETLRRERQE REQLSHPAHF GPRKYCLREC ICEVEGQVPC PAVVPLPREL T GKFQAALR AGAQD

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Macromolecule #9: 28S ribosomal protein S26, mitochondrial

MacromoleculeName: 28S ribosomal protein S26, mitochondrial / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bovine (cattle)
Molecular weightTheoretical: 23.815941 KDa
SequenceString: MLRALSTLGA RPLGRPPAQF LLLARGRKTR HDPPAKSKIG RVATPPAVDP AEFFVLTERY RQYRQTVRAL RQEFVTEVRR KVHEARAGV LAERKALQDA AEHRELMAWN QAENQRLHEL RMARLRQEAR EQEQWQAEEA AREAREAEAW ARLKEQEVLQ L QEDAKNFI ...String:
MLRALSTLGA RPLGRPPAQF LLLARGRKTR HDPPAKSKIG RVATPPAVDP AEFFVLTERY RQYRQTVRAL RQEFVTEVRR KVHEARAGV LAERKALQDA AEHRELMAWN QAENQRLHEL RMARLRQEAR EQEQWQAEEA AREAREAEAW ARLKEQEVLQ L QEDAKNFI TRENLEARVE EALDSPKSYN WAVTREGQVV TPQHKGS

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Macromolecule #10: 28S ribosomal protein S27, mitochondrial

MacromoleculeName: 28S ribosomal protein S27, mitochondrial / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bos taurus (cattle)
Molecular weightTheoretical: 48.017867 KDa
Recombinant expressionOrganism: Bos taurus (cattle)
SequenceString: MAAPMVRRAI FLARNVLLPQ LSLAGKRYLL SAAYEDSRKW EARAKEDCHL ADLASLMDKT YERKLPVSSL TISRFVDNIS SREEIDHAE YYLYKFRHSP NCWYLRNWTI HTWIRQCLKY GAQDKALYTL VNKVQYGIFP DNYTFNLLMD HFIKKENYKD A LSVVFEIM ...String:
MAAPMVRRAI FLARNVLLPQ LSLAGKRYLL SAAYEDSRKW EARAKEDCHL ADLASLMDKT YERKLPVSSL TISRFVDNIS SREEIDHAE YYLYKFRHSP NCWYLRNWTI HTWIRQCLKY GAQDKALYTL VNKVQYGIFP DNYTFNLLMD HFIKKENYKD A LSVVFEIM MQEAFEVPST QLLSLYVLYQ CLAKKTDFSW EEERNFGASL LLPGLKQKNS VGLSSQLYGY ALLGKVELQQ GL RAVYHNM PLLWRPGYLD RALQVMEKVA SSPEDGKLCR EALGVLDRAL KALTAPAQES PEEQPQEGEE SPASEELMEQ LDV EETEQS KLPRYVERYE ALHSKLQALG KVESESLLTL TTQLVKEQLP TCEAEDIATY EQKLQEWHLE LVNLIEREKE MREK ARLKH EARRAAKAAA

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Macromolecule #11: 28S ribosomal protein S34, mitochondrial

MacromoleculeName: 28S ribosomal protein S34, mitochondrial / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bovine (cattle)
Molecular weightTheoretical: 25.880799 KDa
SequenceString: MARRKVRPRL IAELARRVRA LREQRERPRD SVRYALDYET LIRPHSGRKL PLRAWVDVRR ESRLLQLLGR LPFFGLGRLV TRKSWLWQH DEPCYWRLTR VRPDYTAQNL DHGKAWGILT FKGKTESEAR EIEQVMHHDW RLVPKHEEAA FTSFTPAPEE T PRPVPYPP ...String:
MARRKVRPRL IAELARRVRA LREQRERPRD SVRYALDYET LIRPHSGRKL PLRAWVDVRR ESRLLQLLGR LPFFGLGRLV TRKSWLWQH DEPCYWRLTR VRPDYTAQNL DHGKAWGILT FKGKTESEAR EIEQVMHHDW RLVPKHEEAA FTSFTPAPEE T PRPVPYPP LLRAMILAER QKNGDPSTEE PMLSLERIRT DPWDYPENQE AKKKTKGTAV

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Macromolecule #12: 28S ribosomal protein S18b, mitochondrial

MacromoleculeName: 28S ribosomal protein S18b, mitochondrial / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bovine (cattle)
Molecular weightTheoretical: 29.217355 KDa
SequenceString: MAASVLNVLL RRLPYFSPFR GAYGVQVPLQ TLCTKAPPED DSLPPIPVSP YEDEPWKYLD SEEYHNRNGS RPVWADYRRN HKGGIPPQR TRKMCIRGNK VAGNPCPICR DQKLHVDFRN VKLLKQFVCA HTGIIFHAPY TGVCMKQHKK LTQAIQKARD H GLLSYHIP ...String:
MAASVLNVLL RRLPYFSPFR GAYGVQVPLQ TLCTKAPPED DSLPPIPVSP YEDEPWKYLD SEEYHNRNGS RPVWADYRRN HKGGIPPQR TRKMCIRGNK VAGNPCPICR DQKLHVDFRN VKLLKQFVCA HTGIIFHAPY TGVCMKQHKK LTQAIQKARD H GLLSYHIP QVEPRDLDFS TSHGAVSATP PAPTLVSGDP WYPWYSWKQP PERELSRLRR LYQGHLREES GPPPESMPKV PL TAPNEAT STEQAGPQSA L

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Macromolecule #13: 28S ribosomal protein S7, mitochondrial

MacromoleculeName: 28S ribosomal protein S7, mitochondrial / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bovine (cattle)
Molecular weightTheoretical: 28.17901 KDa
SequenceString: MAAPTAKVSR GWSGLALGVR IAVLRLPGLT QVRWSRYGPE YQDPQIDKEY YRKPLAQLTE EETYERELRK TQVIKAAPAT KTSSVFEDP VISKFTNMMM KGGNKILARS LMTQTLEAVK RKQFEKYHAA SAEEQATVER NPYTIFHQAL KNCEPVIGLV P ILKGGHFY ...String:
MAAPTAKVSR GWSGLALGVR IAVLRLPGLT QVRWSRYGPE YQDPQIDKEY YRKPLAQLTE EETYERELRK TQVIKAAPAT KTSSVFEDP VISKFTNMMM KGGNKILARS LMTQTLEAVK RKQFEKYHAA SAEEQATVER NPYTIFHQAL KNCEPVIGLV P ILKGGHFY QVPVPLAERR RRFLAMKWMI TECREKKPRR MLMPEKLSQE LLEAFCNRGP VIKRKHDMHK MAEANRALAH YR WW

+
Macromolecule #14: 28S ribosomal protein S9, mitochondrial

MacromoleculeName: 28S ribosomal protein S9, mitochondrial / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bovine (cattle)
Molecular weightTheoretical: 45.302754 KDa
SequenceString: MAAPSVSCGA AVPYRLFLAG RVSFAREQGL WKAAASGLQT GTRCQILRLK HSPAVTTTKN VAALRRESYT VDFIKKQIEE FNIGKRHLA NMMGEDPETF TQEDVDRAIT YLFPSGLFEK RARPIMKHPE EIFPKQRAVQ WGEDGRPFHF LFYTGKQSYY S LMHEAYGK ...String:
MAAPSVSCGA AVPYRLFLAG RVSFAREQGL WKAAASGLQT GTRCQILRLK HSPAVTTTKN VAALRRESYT VDFIKKQIEE FNIGKRHLA NMMGEDPETF TQEDVDRAIT YLFPSGLFEK RARPIMKHPE EIFPKQRAVQ WGEDGRPFHF LFYTGKQSYY S LMHEAYGK VLHAEERQDQ LRAKGLFSEK SKSKDLIGSR WLIKEELEEM LVEKLSDQDY AQFIRLLERL SALPCDAAEE EF VGRFRRT VTVQSKKHLI EPLQYDEQGM AFSTGQGKRK TANAEAVVYG HGSGKIEING VDYLLYFPVT QDREQLMFPF HFL DRLGKH DVTCTVSGGG RSSQAGAIRL AMSRALCSFI TEDEVEWMRQ AGLLTTDPRV RERKKPGQEG ARRKFTWKKR

+
Macromolecule #15: 28S ribosomal protein S10, mitochondrial

MacromoleculeName: 28S ribosomal protein S10, mitochondrial / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bovine (cattle)
Molecular weightTheoretical: 23.069605 KDa
SequenceString: MAVRAVFGAL GRRLWQGSKN FSVSSSRSNI AKNDGFLLST SMKWVQFSNL HVDVPKDLTK PTITISDEPD TLYKRLSVLV KGHDKAVLD SYEYFAVLAA KELGISVKVH EPPRKIERFT LLKSVHIFKK HRVQYEMRTL YRCLELEHLT GSTADVYLEY I QRNLPEGV ...String:
MAVRAVFGAL GRRLWQGSKN FSVSSSRSNI AKNDGFLLST SMKWVQFSNL HVDVPKDLTK PTITISDEPD TLYKRLSVLV KGHDKAVLD SYEYFAVLAA KELGISVKVH EPPRKIERFT LLKSVHIFKK HRVQYEMRTL YRCLELEHLT GSTADVYLEY I QRNLPEGV AMEVTKTRLE QLPEHIKKPV WETTPEEKGD SKS

+
Macromolecule #16: 28S ribosomal protein S14, mitochondrial

MacromoleculeName: 28S ribosomal protein S14, mitochondrial / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bovine (cattle)
Molecular weightTheoretical: 15.073671 KDa
SequenceString:
MATSMLGSLL RIVRQVVPSS ASGQARSYYV DWRMLRDVKR RKMAYEYADE RLRINSLRKN TILPKHLQEV ADEEIAALPR DSCPVRIRN RCVMTSRPRG VKRRWRLSRI VFRHLADHGQ LSGIQRAIW

+
Macromolecule #17: DAP3 protein

MacromoleculeName: DAP3 protein / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bovine (cattle)
Molecular weightTheoretical: 40.505719 KDa
SequenceString: ISRTLENDPA KHGEQHVGQH YNISIQELKT VFPHGLPPRF VMQVKTFNEA CLMVRKPALE LLHYLKNTNF AHPAVRYVLY GEKGTGKTL SLCHIIHFCA KQDWLILHIP DAHLWVKNCR DLLQSTYNKQ RFDQPLEASI WLKNFKTANE RFLSQIKVQD K YIWNKRES ...String:
ISRTLENDPA KHGEQHVGQH YNISIQELKT VFPHGLPPRF VMQVKTFNEA CLMVRKPALE LLHYLKNTNF AHPAVRYVLY GEKGTGKTL SLCHIIHFCA KQDWLILHIP DAHLWVKNCR DLLQSTYNKQ RFDQPLEASI WLKNFKTANE RFLSQIKVQD K YIWNKRES TEKGSPLAEV VEQGIMRVRN ATDAVGIVLK ELKRQSSLGV FRLLVAVDGV NALWGRTTLK REDKSPITPE EL ALIYNLR KMVKNDWQGG AIVLTVSQTG SLFKPRKAYL PQELLGKEGF DTLDPFIPIL VSNYNPKEFE GCIQYYLENN WLQ HEKAHT EEGKKELLFL SNRNPGLLER LCAYL

+
Macromolecule #18: 28S ribosomal protein S31, mitochondrial

MacromoleculeName: 28S ribosomal protein S31, mitochondrial / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bovine (cattle)
Molecular weightTheoretical: 43.740461 KDa
SequenceString: MFPRVSAVLP FRPLSRLPLC SAGPEASAAT VVPLASPHGT VRTKCNIQRY FGTNSVIYSK KDDKSVPACE ISKETENQGS TKENKKKDL VNIIKGMKVE LSTVNVQTTK PPNRGQLKSL EAAIRRLQKS PEDAPQKSKS LSPELVAAAT AVADSLPFDK Q TTKSELLR ...String:
MFPRVSAVLP FRPLSRLPLC SAGPEASAAT VVPLASPHGT VRTKCNIQRY FGTNSVIYSK KDDKSVPACE ISKETENQGS TKENKKKDL VNIIKGMKVE LSTVNVQTTK PPNRGQLKSL EAAIRRLQKS PEDAPQKSKS LSPELVAAAT AVADSLPFDK Q TTKSELLR QLRQHEEDSK AQKDGEKPKI SFSNIISDMK VARSSTARAS TRPVHQIQFD EGADDFVDRE ETADLRKRFR KN IFKGKRL NIFELKPVTE EAPETEAAPS LWDVEFAKQL AAVTEQPFQN GFEEMIQWTK EGKLWEFPIN NEAGFDDDGS EFH EHIFLD KYLEGFPKQG PIRHFMELVT CGLSKNPYLS VKQKVEHIEW FRNYFNEKQD ILKESGINFS

+
Macromolecule #19: 28S ribosomal protein S33, mitochondrial

MacromoleculeName: 28S ribosomal protein S33, mitochondrial / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bovine (cattle)
Molecular weightTheoretical: 12.480582 KDa
SequenceString:
MSSLSEYALR MSRLSARLFS EVARPTDSKS MKVVKLFSEQ PLAKRKETYD WYPNHNTYFA LMGILRSVGL YRDEHQDFKD EQLRLKKLR GKVKPRKGEG KRAAKKK

+
Macromolecule #20: 28S ribosomal protein S35, mitochondrial

MacromoleculeName: 28S ribosomal protein S35, mitochondrial / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bovine (cattle)
Molecular weightTheoretical: 37.129746 KDa
SequenceString: MAAPSLPAWL ALQTRARTLR AFSTAVSPVT GAQRLSPPTT ERTSKHERAP RRKALPPRTE KMSVDQDWPS VYPVAAPFKP SAVPLPVRM GYPVKRGVPM AKEGNLELLK IPNFLHLTPV AIKKHCEALK DFCTEWPAAL DSDEKCEKHF PIEIDTADYI S SGPSIRNP ...String:
MAAPSLPAWL ALQTRARTLR AFSTAVSPVT GAQRLSPPTT ERTSKHERAP RRKALPPRTE KMSVDQDWPS VYPVAAPFKP SAVPLPVRM GYPVKRGVPM AKEGNLELLK IPNFLHLTPV AIKKHCEALK DFCTEWPAAL DSDEKCEKHF PIEIDTADYI S SGPSIRNP KARVVTLRVK LSSLNLDDHA KKKLIKLVGD RYCKSTDVLT IKTDRCPLKR QNYDYAMYLL TVLYHESWKT EE WEKKKTE ADMEEYVWKD SASEKNILET LFQIKAAEKN TELSKEELLS TKEVEDYKNS VVSLKNEGDN ENTISQYKES VKR LLHLM

+
Macromolecule #21: Coiled-coil-helix-coiled-coil-helix domain containing 1

MacromoleculeName: Coiled-coil-helix-coiled-coil-helix domain containing 1
type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bovine (cattle)
Molecular weightTheoretical: 13.581974 KDa
SequenceString:
MATPSLRGRL ARLGNPRKPI LKPNKPLILA NHVGERRREK GEATCITEMS IMMACWKQNE FRDEACKKEI RDFFDCASRA EAARKMRSI QEDLGELGSL PPRKLNKLLQ RFPNKPHLS

+
Macromolecule #22: Pentatricopeptide repeat domain-containing protein 3, mitochondrial

MacromoleculeName: Pentatricopeptide repeat domain-containing protein 3, mitochondrial
type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bovine (cattle)
Molecular weightTheoretical: 60.587832 KDa
SequenceString: MASVASARWL RVSCGLCVPL TARRAGPCGR TPSSRFYSGS AAVPKDEGAD IAGTEEVVIP KKKTWDKVAV LQALASTVHR DTTAAPYAF QDDPYLIPTS SVESHSFLLA KKSGENAAKF IINSYPKYFQ KDIAEPHIPC LMPE(UNK)(UNK)(UNK) (UNK)(UNK) (UNK) ...String:
MASVASARWL RVSCGLCVPL TARRAGPCGR TPSSRFYSGS AAVPKDEGAD IAGTEEVVIP KKKTWDKVAV LQALASTVHR DTTAAPYAF QDDPYLIPTS SVESHSFLLA KKSGENAAKF IINSYPKYFQ KDIAEPHIPC LMPE(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)SLAT YHHIIQLFY(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)MFFQSA MRVCSSLRDL ELAYQVHGLL NTGDNRKFIG PDPRRNFYYS KFFSLLCLME QIDVTLKWY KDLIPSVFFP HSQTLIDLLQ ALDVANRLEM IPQIWKDSKE YGHTFRSDLK EEILMLMARD QHPPELQAAF ADCAADIKST YESQDARQT ASEWPANSLN YIAILFLRAG RTQEAWKMLG LFRKHNKIPR NELLNEFMDS AKASSSPAQA VEVVKLANSF S LPICEGLT QRLTADFTLS QEQKEALGDL TALTSDSESD SDSDTSKDK

+
Macromolecule #23: 28S ribosomal protein S2, mitochondrial

MacromoleculeName: 28S ribosomal protein S2, mitochondrial / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bovine (cattle)
Molecular weightTheoretical: 31.775492 KDa
SequenceString: MATGAVLPRL LGAGVRAAPR GRAAQRGRTL GSAAAAAARE PERDSDRSAR ILSEPLKHSD FFNVKELFSV RSLFNARVHL GHKAGCRHR FMEPYIFGSR LGQDIIDLEQ TATHLQLALN FTAHVAFRGG IILFVSRARQ FSHLIESTAR SCGEYAHTRY F KGGLLTNA ...String:
MATGAVLPRL LGAGVRAAPR GRAAQRGRTL GSAAAAAARE PERDSDRSAR ILSEPLKHSD FFNVKELFSV RSLFNARVHL GHKAGCRHR FMEPYIFGSR LGQDIIDLEQ TATHLQLALN FTAHVAFRGG IILFVSRARQ FSHLIESTAR SCGEYAHTRY F KGGLLTNA PLLLGARVRL PDLIIFLHTL NNVFEPHVAV RDAAKMSIPT VGVVDTNCNP CLITYPVPGN DDSPPAVQLF CQ LFQTAVT RAKEKRRQLE ALYRLQGAPG PHPANPAAPG APSPGAQAQL GMGHSP

+
Macromolecule #24: 28S ribosomal protein S6, mitochondrial

MacromoleculeName: 28S ribosomal protein S6, mitochondrial / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bovine (cattle)
Molecular weightTheoretical: 14.171551 KDa
SequenceString:
MPRYELALIL KAMQRPETAA ALKRTLEALM DRGAVVRSLE NLGERTLPYK MSAHSQRHTR GGYFLVDFYA PTTTVASIME HLSRDIDVI RPNVVKHPLT QEVKECEGIV PVPLEEKLYS TKKRK

+
Macromolecule #25: 28S ribosomal protein S11, mitochondrial

MacromoleculeName: 28S ribosomal protein S11, mitochondrial / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bovine (cattle)
Molecular weightTheoretical: 20.869008 KDa
SequenceString: MQVLRNSGSW LLRSWAWPPT TRVVAGVPAP TIHMSAQQMQ DAAAKEEVEK AETPAPAPSR SSFSIYPPIP GQESSLRWAG KKFEEIPIA HIKASYNNTQ IHVVSAAHQP LARASCGTEG FRNAKKGTGI AAQTAGIAAA AKATGKGVTH VRVVVKGLGP G RLSAIKGL ...String:
MQVLRNSGSW LLRSWAWPPT TRVVAGVPAP TIHMSAQQMQ DAAAKEEVEK AETPAPAPSR SSFSIYPPIP GQESSLRWAG KKFEEIPIA HIKASYNNTQ IHVVSAAHQP LARASCGTEG FRNAKKGTGI AAQTAGIAAA AKATGKGVTH VRVVVKGLGP G RLSAIKGL TMGGLEVISI TDNTPIPHNG CRPRKARRL

+
Macromolecule #26: 28S ribosomal protein S15, mitochondrial

MacromoleculeName: 28S ribosomal protein S15, mitochondrial / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bovine (cattle)
Molecular weightTheoretical: 29.245316 KDa
SequenceString: MLRAAWRALS SIRTQAVTQP PVLGLPGGGC AKLLSVQRDL PSSLILQAAR GYATQKPVQQ SQEDDPPPSM LLKDYQNVPG IEKVDDVVK RLISLEMANK KEMLKIKKEQ LMSKVVENPK DTSSLEAQIV ALTVKIRSYE EHMQKHQKDK AHKRYLLMSI D KRQKMLKN ...String:
MLRAAWRALS SIRTQAVTQP PVLGLPGGGC AKLLSVQRDL PSSLILQAAR GYATQKPVQQ SQEDDPPPSM LLKDYQNVPG IEKVDDVVK RLISLEMANK KEMLKIKKEQ LMSKVVENPK DTSSLEAQIV ALTVKIRSYE EHMQKHQKDK AHKRYLLMSI D KRQKMLKN LRKTNYPVFE KTCKELGIEY TFPPPYHRKI HRRLATKKAL CIRVFQEVQK LKKQKRALKT AAAAAQKQGQ RN PESPSEA RSEAIKETQ

+
Macromolecule #27: 28S ribosomal protein S18c, mitochondrial

MacromoleculeName: 28S ribosomal protein S18c, mitochondrial / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bovine (cattle)
Molecular weightTheoretical: 16.240283 KDa
SequenceString:
MAAVLGLCGG LGKRKFTRFP TAFVCLTNSG TRAVLWRSCS QCKQVTSSED LPIPMENPYK EPLKKCILCE KRVDYKNVQL LSQFISPFT GCIYGRHITG LCGKKQREIT KAIKRAQILG FMPVTYKDPA YLKDPKVCNI RYRE

+
Macromolecule #28: 28S ribosomal protein S21, mitochondrial

MacromoleculeName: 28S ribosomal protein S21, mitochondrial / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bovine (cattle)
Molecular weightTheoretical: 10.697583 KDa
SequenceString:
MAKHLKFIAR TVMVQEGNVE GAYRTLNRIL TMDGLIEDIK RRRYYEKPCR RRQRESYETC RRIYNMEMAR KINFLMRKNR ADPWQGC

+
Macromolecule #29: 28S ribosomal protein S23, mitochondrial

MacromoleculeName: 28S ribosomal protein S23, mitochondrial / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bovine (cattle)
Molecular weightTheoretical: 21.625533 KDa
SequenceString:
MAGSRLETVG SIFTRTRDLI RAGVLKEKPL WFDVYNAFPP LREPVFRRPR LRYGKAKSPT QDIYYHEDQI RAKFYAAYGS GPKAFDLFN PNFKSTCQRF VEKYIELQKL GETDEEKLFV EAGKALLAEG VILRRVEKAR TQQEGSQVSR KSESMGVESQ T ALEENPPL KEVPQAQHLE SPGEESKGLS PP

+
Macromolecule #30: 28S ribosomal protein S28, mitochondrial

MacromoleculeName: 28S ribosomal protein S28, mitochondrial / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bovine (cattle)
Molecular weightTheoretical: 21.077977 KDa
SequenceString:
MAALCRTRAV TAESHFLRVF LFSRSCRGAG TESGSGSESS NSTEPRPRPG GFASALERHS ELQRKAELAR TRGSPKNVES FASMLRHSP LTQMGPAKDK IVIGRIFHIV ENDLYIDFGG KFHCVCKRPE VDGEKYQKGT RVRLRLLDLE LTSRFLGATT D TTILEAEA VLLGLQESKD SKSKEERREN K

+
Macromolecule #31: Aurora kinase A interacting protein 1

MacromoleculeName: Aurora kinase A interacting protein 1 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bovine (cattle)
Molecular weightTheoretical: 22.968365 KDa
SequenceString: MFLMRLTSQL LRAVPRAGCS GPWPVLGVLG RHACRPCYST KPTGPSGVAS LPGRRVHMEL EEMLVPRKMS ISPLESWLTI RYLLPRLDT GAPGTVSPAQ LYECPPSRVG EGVEQGGKDV FDAPQMQCRN VLKIRRRKMN HHKYRKLVKR TRFLRRKVRE A RLKRKQMK ...String:
MFLMRLTSQL LRAVPRAGCS GPWPVLGVLG RHACRPCYST KPTGPSGVAS LPGRRVHMEL EEMLVPRKMS ISPLESWLTI RYLLPRLDT GAPGTVSPAQ LYECPPSRVG EGVEQGGKDV FDAPQMQCRN VLKIRRRKMN HHKYRKLVKR TRFLRRKVRE A RLKRKQMK FERDLRRIWQ KAGLKEAPPG WQTPKIYLKG K

+
Macromolecule #32: Translation initiation factor IF-3, mitochondrial

MacromoleculeName: Translation initiation factor IF-3, mitochondrial / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 28.21316 KDa
SequenceString: TAPAQLSPIA SAPRLSFLIH AKAFSTAEDT QNEGKKTKKN KTAFSNVGRK ISQRVIHLFD EKGNDLGNMH RANVIRLMDE RDLRLVQRN TSTEPAEYQL MTGLQILQER QRLREMEKAN PKTGPTLRKE LILSSNIGQH DLDTKTKQIQ QWIKKKHLVQ I TIKKGKNV ...String:
TAPAQLSPIA SAPRLSFLIH AKAFSTAEDT QNEGKKTKKN KTAFSNVGRK ISQRVIHLFD EKGNDLGNMH RANVIRLMDE RDLRLVQRN TSTEPAEYQL MTGLQILQER QRLREMEKAN PKTGPTLRKE LILSSNIGQH DLDTKTKQIQ QWIKKKHLVQ I TIKKGKNV DVSENEMEEI FHQILQTMPG IATFSSRPQA VQGGKALMCV LRAFSKNEEK AYKETQETQE RDTLNKDHGN DK ESNVLHQ

-
Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

BufferpH: 7.6
GridDetails: unspecified
VitrificationCryogen name: ETHANE

-
Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: OTHER
Electron opticsIllumination mode: OTHER / Imaging mode: OTHER
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 70.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

-
Image processing

Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

3jd5

Initial angle assignmentType: PROJECTION MATCHING
Final angle assignmentType: MAXIMUM LIKELIHOOD
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.32 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 198355

-
Atomic model buiding 1

Initial modelPDB ID:

3jd5

RefinementProtocol: OTHER / Overall B value: 97
Output model

PDB-6neq:
Structure of human mitochondrial translation initiation factor 3 bound to the small ribosomal subunit-Class-II

+
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