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- EMDB-0441: Conformational switches control early maturation of the eukaryoti... -

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Basic information

Entry
Database: EMDB / ID: EMD-0441
TitleConformational switches control early maturation of the eukaryotic small ribosomal subunit
Map datamasked, sharpened map
Sample
  • Complex: 5' ETS particle
    • RNA: x 3 types
    • Protein or peptide: x 25 types
KeywordsRibosome assembly / RIBOSOME
Function / homology
Function and homology information


RNA fragment catabolic process / rRNA 2'-O-methylation / t-UTP complex / Mpp10 complex / snoRNA guided rRNA 2'-O-methylation / Pwp2p-containing subcomplex of 90S preribosome / rRNA modification / septum digestion after cytokinesis / snRNA binding / tRNA re-export from nucleus ...RNA fragment catabolic process / rRNA 2'-O-methylation / t-UTP complex / Mpp10 complex / snoRNA guided rRNA 2'-O-methylation / Pwp2p-containing subcomplex of 90S preribosome / rRNA modification / septum digestion after cytokinesis / snRNA binding / tRNA re-export from nucleus / box C/D sno(s)RNA 3'-end processing / endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rRNA methyltransferase activity / rDNA heterochromatin / endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / regulation of transcription by RNA polymerase I / box C/D methylation guide snoRNP complex / U4/U6 snRNP / positive regulation of rRNA processing / tRNA export from nucleus / sno(s)RNA-containing ribonucleoprotein complex / SUMOylation of RNA binding proteins / protein localization to nucleolus / U4 snRNA binding / O-methyltransferase activity / rRNA methylation / histone H2AQ104 methyltransferase activity / U4 snRNP / U3 snoRNA binding / snoRNA binding / precatalytic spliceosome / preribosome, small subunit precursor / establishment of cell polarity / Major pathway of rRNA processing in the nucleolus and cytosol / positive regulation of transcription by RNA polymerase I / spliceosomal complex assembly / 90S preribosome / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / U4/U6 x U5 tri-snRNP complex / RNA endonuclease activity / Transferases; Transferring one-carbon groups; Methyltransferases / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / small-subunit processome / spliceosomal complex / mRNA splicing, via spliceosome / rRNA processing / peroxisome / ribosomal small subunit biogenesis / ribosomal small subunit assembly / tRNA binding / rRNA binding / mRNA binding / nucleolus / mitochondrion / RNA binding / nucleoplasm / identical protein binding / nucleus / cytoplasm
Similarity search - Function
U3 small nucleolar RNA-associated protein 8 / : / Utp8, N-terminal beta propeller / Utp8, C-terminal / U3 snoRNA associated / U3 snoRNA associated / WD repeat-containing protein 75 second beta-propeller / U3 small nucleolar RNA-associated protein 6 / U3 small nucleolar RNA-associated protein 4 / : ...U3 small nucleolar RNA-associated protein 8 / : / Utp8, N-terminal beta propeller / Utp8, C-terminal / U3 snoRNA associated / U3 snoRNA associated / WD repeat-containing protein 75 second beta-propeller / U3 small nucleolar RNA-associated protein 6 / U3 small nucleolar RNA-associated protein 4 / : / U3 small nucleolar RNA-associated protein 6 / Sof1-like protein / BING4, C-terminal domain / rRNA-processing protein Fcf1, PIN domain / WD repeat-containing protein WDR46/Utp7 / : / : / Sof1-like domain / BING4CT (NUC141) domain / BING4CT (NUC141) domain / : / U3 small nucleolar RNA-associated protein 15, C-terminal / UTP15 C terminal / rRNA-processing protein Fcf1/Utp23 / Fcf1 / Nucleolar protein 58/56, N-terminal / U3 small nucleolar RNA-associated protein 18 / NOP5NT (NUC127) domain / : / BP28, C-terminal domain / U3 small nucleolar RNA-associated protein 10, N-terminal / U3 small nucleolar RNA-associated protein 10 / : / BP28CT (NUC211) domain / U3 small nucleolar RNA-associated protein 10 / HEATR1-like, HEAT repeats / BP28CT (NUC211) domain / U3 small nucleolar RNA-associated protein 13, C-terminal / Periodic tryptophan protein 2 / Utp13 specific WD40 associated domain / Small-subunit processome, Utp12 / Dip2/Utp12 Family / Small-subunit processome, Utp21 / U3 small nucleolar ribonucleoprotein complex, subunit Mpp10 / Mpp10 protein / Utp21 specific WD40 associated putative domain / Ribosomal RNA-processing protein Rrp9-like / PRP39 N-terminal HAT repeat / Nucleolar protein Nop56/Nop58 / rRNA 2'-O-methyltransferase fibrillarin-like / Fibrillarin, conserved site / Fibrillarin / Fibrillarin signature. / Fibrillarin / Large family of predicted nucleotide-binding domains / Anaphase-promoting complex subunit 4, WD40 domain / PIN domain / NOSIC / NOSIC (NUC001) domain / Nop domain / Nop domain superfamily / Nop, C-terminal domain / snoRNA binding domain, fibrillarin / Nop domain profile. / H/ACA ribonucleoprotein complex, subunit Nhp2-like / Anaphase-promoting complex subunit 4 WD40 domain / HEAT repeat profile. / HEAT, type 2 / HAT (Half-A-TPR) repeat / HAT (Half-A-TPR) repeats / PIN-like domain superfamily / Quinoprotein alcohol dehydrogenase-like superfamily / Ribosomal protein L7Ae conserved site / Ribosomal protein L7Ae signature. / Ribosomal protein L7Ae/L8/Nhp2 family / : / Ribosomal protein L7Ae/L30e/S12e/Gadd45 / Ribosomal protein L7Ae/L30e/S12e/Gadd45 family / 50S ribosomal protein L30e-like / Armadillo-like helical / Ribosomal protein S4/S9 N-terminal domain / Ribosomal protein S4/S9 N-terminal domain / Ribosomal protein S4/S9, N-terminal / Ribosomal protein S4/S9 / S4 RNA-binding domain profile. / Tetratricopeptide-like helical domain superfamily / S4 RNA-binding domain / S4 domain / RNA-binding S4 domain / RNA-binding S4 domain superfamily / Armadillo-type fold / G-protein beta WD-40 repeat / WD40 repeat, conserved site / Trp-Asp (WD) repeats signature. / WD domain, G-beta repeat / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily
Similarity search - Domain/homology
rRNA 2'-O-methyltransferase fibrillarin / Periodic tryptophan protein 2 / U3 small nucleolar ribonucleoprotein protein IMP3 / Protein SOF1 / U3 small nucleolar RNA-associated protein 9 / 13 kDa ribonucleoprotein-associated protein / U3 small nucleolar RNA-associated protein 7 / U3 small nucleolar RNA-associated protein 18 / U3 small nucleolar RNA-associated protein 10 / U3 small nucleolar RNA-associated protein MPP10 ...rRNA 2'-O-methyltransferase fibrillarin / Periodic tryptophan protein 2 / U3 small nucleolar ribonucleoprotein protein IMP3 / Protein SOF1 / U3 small nucleolar RNA-associated protein 9 / 13 kDa ribonucleoprotein-associated protein / U3 small nucleolar RNA-associated protein 7 / U3 small nucleolar RNA-associated protein 18 / U3 small nucleolar RNA-associated protein 10 / U3 small nucleolar RNA-associated protein MPP10 / U3 small nucleolar RNA-associated protein 8 / U3 small nucleolar RNA-associated protein 6 / NET1-associated nuclear protein 1 / U3 small nucleolar RNA-associated protein 5 / U3 small nucleolar RNA-associated protein 15 / rRNA-processing protein FCF1 / U3 small nucleolar RNA-associated protein 13 / U3 small nucleolar RNA-associated protein 21 / Ribosomal RNA-processing protein 9 / U3 small nucleolar RNA-associated protein 4 / U3 small nucleolar RNA-associated protein 16 / U3 small nucleolar RNA-associated protein 12 / Nucleolar protein 56 / Nucleolar protein 58
Similarity search - Component
Biological speciesSaccharomyces cerevisiae BY4741 (yeast)
Methodsingle particle reconstruction / cryo EM / Resolution: 4.3 Å
AuthorsHunziker M / Barandun J
Funding support United States, Switzerland, 2 items
OrganizationGrant numberCountry
National Institutes of Health/Office of the Director1DP2GM123459 United States
Swiss National Science Foundation155515 Switzerland
CitationJournal: Elife / Year: 2019
Title: Conformational switches control early maturation of the eukaryotic small ribosomal subunit.
Authors: Mirjam Hunziker / Jonas Barandun / Olga Buzovetsky / Caitlin Steckler / Henrik Molina / Sebastian Klinge /
Abstract: Eukaryotic ribosome biogenesis is initiated with the transcription of pre-ribosomal RNA at the 5' external transcribed spacer, which directs the early association of assembly factors but is absent ...Eukaryotic ribosome biogenesis is initiated with the transcription of pre-ribosomal RNA at the 5' external transcribed spacer, which directs the early association of assembly factors but is absent from the mature ribosome. The subsequent co-transcriptional association of ribosome assembly factors with pre-ribosomal RNA results in the formation of the small subunit processome. Here we show that stable rRNA domains of the small ribosomal subunit can independently recruit their own biogenesis factors in vivo. The final assembly and compaction of the small subunit processome requires the presence of the 5' external transcribed spacer RNA and all ribosomal RNA domains. Additionally, our cryo-electron microscopy structure of the earliest nucleolar pre-ribosomal assembly - the 5' external transcribed spacer ribonucleoprotein - provides a mechanism for how conformational changes in multi-protein complexes can be employed to regulate the accessibility of binding sites and therefore define the chronology of maturation events during early stages of ribosome assembly.
History
DepositionDec 13, 2018-
Header (metadata) releaseFeb 20, 2019-
Map releaseJun 19, 2019-
UpdateMar 20, 2024-
Current statusMar 20, 2024Processing site: RCSB / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.0203
  • Imaged by UCSF Chimera
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  • Surface view colored by radius
  • Surface level: 0.0203
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6nd4
  • Surface level: 0.0203
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_0441.png

Downloads & links

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Map

FileDownload / File: emd_0441.map.gz / Format: CCP4 / Size: 178 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotationmasked, sharpened map
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.6 Å/pix.
x 360 pix.
= 576. Å		1.6 Å/pix.
x 360 pix.
= 576. Å		1.6 Å/pix.
x 360 pix.
= 576. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.6 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.0203 / Movie #1: 0.0203
Minimum - Maximum-0.04939924 - 0.09136246
Average (Standard dev.)0.0001420385 (±0.001903109)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions360360360
Spacing360360360
CellA=B=C: 576.0 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.61.61.6
M x/y/z360360360
origin x/y/z0.0000.0000.000
length x/y/z576.000576.000576.000
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS360360360
D min/max/mean-0.0490.0910.000

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Supplemental data

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Additional map: unsharpened map

Fileemd_0441_additional.map
Annotationunsharpened map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : 5' ETS particle

EntireName: 5' ETS particle
Components
  • Complex: 5' ETS particle
    • RNA: 5'ETS rRNA
    • RNA: 18S rRNA 5' domain start
    • RNA: U3 snoRNA
    • Protein or peptide: Utp17
    • Protein or peptide: Utp8
    • Protein or peptide: Utp15
    • Protein or peptide: Utp9
    • Protein or peptide: Utp5
    • Protein or peptide: Utp10
    • Protein or peptide: Utp4
    • Protein or peptide: Utp1
    • Protein or peptide: Utp6
    • Protein or peptide: Utp12
    • Protein or peptide: Utp13
    • Protein or peptide: Utp18
    • Protein or peptide: Utp21
    • Protein or peptide: Sof1
    • Protein or peptide: Utp7
    • Protein or peptide: Imp3
    • Protein or peptide: Mpp10
    • Protein or peptide: Bud21
    • Protein or peptide: Nop56
    • Protein or peptide: Nop58
    • Protein or peptide: Nop1.1
    • Protein or peptide: Snu13
    • Protein or peptide: Rrp9
    • Protein or peptide: Utp24
    • Protein or peptide: Unidentified fragment

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Supramolecule #1: 5' ETS particle

SupramoleculeName: 5' ETS particle / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Saccharomyces cerevisiae BY4741 (yeast)

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Macromolecule #1: 5'ETS rRNA

MacromoleculeName: 5'ETS rRNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Saccharomyces cerevisiae BY4741 (yeast)
Molecular weightTheoretical: 220.317 KDa
SequenceString: AUGCGAAAGC AGUUGAAGAC AAGU(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N) (N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)GCUU GUCGUUCGUU AUGUUUUUGU AAAUGGCCUC GUCAAAC GG UGGAGAGAGU ...String:
AUGCGAAAGC AGUUGAAGAC AAGU(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N) (N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)GCUU GUCGUUCGUU AUGUUUUUGU AAAUGGCCUC GUCAAAC GG UGGAGAGAGU CGCUAGGUGA UCGUCAGAUC UGCCUAGUCU CUAUACAGCG UGUUUAAUUG ACAUGGGUUG AUGCGUAU U GAGAGAUACA AUUUGGGAAG AAAUUCCCAG AGUGUGUUUC UUUUGCGUUU AACCUGAACA GUCUCAUCGU GGGCAUCUU GCGAUUCCAU UGGUGAGCAG CGAAGGAUUU GGUGGAUUAC UAGCUAAUAG CAAUCUAUUU CAAAGAAUUC AAACUUGGGG GAAUGCCUU GUUGAAUAGC CGGUCGCAAG ACUGUGAUUC UUCAAGUGUA ACCUCCUCUC AAAUCAGCGA UAUCAAACGU A CCAUUCCG UGAAACACCG GGGUAUCUGU UUGGUGGAAC CUGAUUAGAG GAAACUCAAA GAGUGCUAUG GUAUGGUGAC GG AGUGCGC UGGUCAAGAG UGUAAAAGCU UUUUGAACAG AGAGCAUUUC CGGCAGCAGA GAGACCUGAA AAAGCAAUUU UUC UGGAAU UUCAGCUGUU (N)(N)(N)(N)(N)(N)(N)(N)(N)(N) AUAAGUAUCU UCUAGCAAGA GGGAAUAGGU GGGA AAAAA AAAAAGAGAU UUCGGUUUCU UUCUUUUUUA CUGCUUGUUG CUUCUUCUUU UAAGAUAGU

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Macromolecule #2: 18S rRNA 5' domain start

MacromoleculeName: 18S rRNA 5' domain start / type: rna / ID: 2 / Number of copies: 1
Source (natural)Organism: Saccharomyces cerevisiae BY4741 (yeast)
Molecular weightTheoretical: 3.727073 KDa
SequenceString:
(N)(N)(N)(N)(N)(N)(N)(N)UG AUCCU

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Macromolecule #3: U3 snoRNA

MacromoleculeName: U3 snoRNA / type: rna / ID: 3 / Number of copies: 1
Source (natural)Organism: Saccharomyces cerevisiae BY4741 (yeast)
Molecular weightTheoretical: 46.932852 KDa
SequenceString:
AGGAUCAGGA AUCGUCACUC UUUGACUCUU CAAAAGAGCC ACUGAAUCCA ACUUGGUUGA UGAGUCCCAU AACCUUUGUA CCCAGUGAG AAACCGUAUG GCGCGAUGAU CUACCCAUGG GUGGGUACAA AUGGCAGUCU GACAAGU

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Macromolecule #4: Utp17

MacromoleculeName: Utp17 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae BY4741 (yeast)
Molecular weightTheoretical: 96.36357 KDa
SequenceString: MTQSLGIEQY KLSVVSGGKP ALNNLSSVTG NKNIARLSQD QRNYIIPFNN QIKVYSVETR QCVKTLKFAN NSLLSGIFLQ EEENNESIV KILLGDITVP QQEDAHLITV FTNNGHVIVL NYKGKLVESP KHFKISLADE KLANVFHSEG NYRILTTFKD P SQKAHNSL ...String:
MTQSLGIEQY KLSVVSGGKP ALNNLSSVTG NKNIARLSQD QRNYIIPFNN QIKVYSVETR QCVKTLKFAN NSLLSGIFLQ EEENNESIV KILLGDITVP QQEDAHLITV FTNNGHVIVL NYKGKLVESP KHFKISLADE KLANVFHSEG NYRILTTFKD P SQKAHNSL QSYRLYALTF DDAKKQFEVA HQAEWHNVIL SNISSNGKLL AHMCKDVSTK DHEHKSISVV SLFDDSVNLS FP LGSILSS QTQSLSYNTR YVSSMAIDNM GQQLAVGFAS GVISIVSLAD LQIRLLKWHI DSVLSLSFSH DGSYLLSGGW EKV MSLWQL ETNSQQFLPR LNGIIIDCQV LGPQGNYYSL ILQMTENNSN SDYQFLLLNA SDLTSKLSIN GPLPVFNSTI KHIQ QPISA MNTKNSNSIT SLNHSKKKQS RKLIKSRRQD FTTNVEINPI NKNLYFPHIS AVQIFDFYKN EQVNYQYLTS GVNNS MGKV RFELNLQDPI ITDLKFTKDG QWMITYEIEY PPNDLLSSKD LTHILKFWTK NDNETNWNLK TKVINPHGIS VPITKI LPS PRSVNNSQGC LTADNNGGLK FWSFDSHESN WCLKKISLPN FNHFSNSVSL AWSQDGSLIF HGFDDKLQIL DFDTFKK FE SLENTKTVSE FTLDSEIQTV KLINDTNLIV ATRTTLNAIN LLRGQVINSF DLYPFVNGVY KNGHMDRLIT CDERTGNI A LVINQQLTDL DGVPTINYKS RIIIFDSDLS TKLGNFTHHE YISWIGWNYD TDFIFLDIES TLGVVGT(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)NDEDEEDI ALEFINGEKK DK LVNMNSF TSMFDNIQNV QMDTFFDRVM KVLT

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Macromolecule #5: Utp8

MacromoleculeName: Utp8 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae BY4741 (yeast)
Molecular weightTheoretical: 49.062371 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)RLFK QAIVTCPNLP LNELLEELFS IRNRELLLDI SFRILQDFTR DSIKQEMKKL SKLDVQNFIE FITSGG EDS SPECFNPSQS TQLFQLLSLV LDSIGLFSLE GALLENLTLY IDKQVEIAER NTELWNLIDT KGFQHGFASS TFDNGTS QK RALPTYTMEY LDI

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Macromolecule #6: Utp15

MacromoleculeName: Utp15 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae BY4741 (yeast)
Molecular weightTheoretical: 57.765289 KDa
SequenceString: MSTARPRIIT SKAPLLPQQT TPEQRYWRQY TSAQLVKEHN SVTHISFNPQ HPHDFAVTSS TRVQIFSSRT RQVIKTFSRF KDVVYSASF RSDGKLLCAG DATGLVSVYD SYNPRTILLS INASTHPTHV TKFHTQDNKI LATASDDRVT RLWDISNAYE P QLELTGAT ...String:
MSTARPRIIT SKAPLLPQQT TPEQRYWRQY TSAQLVKEHN SVTHISFNPQ HPHDFAVTSS TRVQIFSSRT RQVIKTFSRF KDVVYSASF RSDGKLLCAG DATGLVSVYD SYNPRTILLS INASTHPTHV TKFHTQDNKI LATASDDRVT RLWDISNAYE P QLELTGAT DYVRTLSFIP AAPHLVATGS YDGLIRLYDT RSSGSTPIYS LNHDQPVENV IAVSPTQIVS CGGNNFKVWD LT SNKKLYE RGNFNKAVTC LDYVENFDSP MQSALIASSL DGHVKVFDPL DNFQVKFGWK FSGPVLSCAV SPSTAQGNRH LVA GLSSGL LAIRTKKKEK RSSDKENAPA SFNKNAKSNN FQRMMRGSEY QGDQEHIIHN DKVRSQRRMR AFERNINQFK WSEA LDNAF VPGMAKELTL TVLQELRKRG KVRVALYGRD ESTLEPLLNW CLKGIEDVRS ASIVADWVAV VLELYGNTLE SSPVL QELM IDLKTKVRHE IHKSKEAQRI EGMLQLLTS

UniProtKB: U3 small nucleolar RNA-associated protein 15

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Macromolecule #7: Utp9

MacromoleculeName: Utp9 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae BY4741 (yeast)
Molecular weightTheoretical: 19.93582 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)DHL ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)DHL SKIFLTISKS ITQNPWNEEN LLPLWLKWLL TLKSGELNSI KDKHTKKNCK HLKSALRSSE EI LPVLLGI QGRLEMLRRQ AKLREDLAQL SMQEGEDDEI EVIEHSNVIS NPLQDQASPV EKLEPDSIVY ANGESDEFVD ASE YKD

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Macromolecule #8: Utp5

MacromoleculeName: Utp5 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae BY4741 (yeast)
Molecular weightTheoretical: 72.079445 KDa
SequenceString: MDSPVLQSAY DPSGQYLCYV TVALDKQRVG VQPTQRATSS GVDTVWNENF LYLEDSKLKV TCLKWVNLAS SDTVAIILGM NNGEIWLYS VLANEVTYKF TTGNSYEIKD IDLMGNQLWC IDSSDAFYQF DLLQFKLLQH FRINNCVQLN KLTIVPAGDS V AQLLVASH ...String:
MDSPVLQSAY DPSGQYLCYV TVALDKQRVG VQPTQRATSS GVDTVWNENF LYLEDSKLKV TCLKWVNLAS SDTVAIILGM NNGEIWLYS VLANEVTYKF TTGNSYEIKD IDLMGNQLWC IDSSDAFYQF DLLQFKLLQH FRINNCVQLN KLTIVPAGDS V AQLLVASH SISLIDIEEK KVVMTFPGHV SPVSTLQVIT NEFFISGAEG DRFLNVYDIH SGMTKCVLVA ESDIKELSHS GQ ADSIAVT TEDGSLEIFV DPLVSSSTKK RGNKSKKSSK KIQIVSKDGR KVPIYNAFIN KDLLNVSWLQ NATMPYFKNL QWR EIPNEY TVEISLNWNN KNKSADRDLH GKDLASATNY VEGNARVTSG DNFKHVDDAI KSWERELTSL EQEQAKPPQA NELL TETFG DKLESSTVAR ISGKKTNLKG SNLKTATTTG TVTVILSQAL QSNDHSLLET VLNNRDERVI RDTIFRLKPA LAVIL LERL AERIARQTHR QGPLNVWVKW CLIIHGGYLV SIPNLMSTLS SLHSTLKRRS DLLPRLLALD ARLDCTINKF KTLNYE AGD IHSSEPVVEE DEDDVEYNEE LDDAGLIEDG EESYGSEEEE EGDSDNEEEQ KHTSSKQDGR LETEQSDGEE EAGYSDV EM E

UniProtKB: U3 small nucleolar RNA-associated protein 5

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Macromolecule #9: Utp10

MacromoleculeName: Utp10 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae BY4741 (yeast)
Molecular weightTheoretical: 200.298984 KDa
SequenceString: MSSLSDQLAQ VASNNATVAL DRKRRQKLHS ASLIYNSKTA ATQDYDFIFE NASKALEELS QIEPKFAIFS RTLFSESSIS LDRNVQTKE EIKDLDNAIN AYLLLASSKW YLAPTLHATE WLVRRFQIHV KNTEMLLLST LNYYQTPVFK RILSIIKLPP L FNCLSNFV ...String:
MSSLSDQLAQ VASNNATVAL DRKRRQKLHS ASLIYNSKTA ATQDYDFIFE NASKALEELS QIEPKFAIFS RTLFSESSIS LDRNVQTKE EIKDLDNAIN AYLLLASSKW YLAPTLHATE WLVRRFQIHV KNTEMLLLST LNYYQTPVFK RILSIIKLPP L FNCLSNFV RSEKPPTALT MIKLFNDMDF LKLYTSYLDQ CIKHNATYTN QLLFTTCCFI NVVAFNSNND EKLNQLVPIL LE ISAKLLA SKSKDCQIAA HTILVVFATA LPLKKTIILA AMETILSNLD AKEAKHSALL TICKLFQTLK GQGNVDQLPS KIF KLFDSK FDTVSILTFL DKEDKPVCDK FITSYTRSIA RYDRSKLNII LSLLKKIRLE RYEVRLIITD LIYLSEILED KSQL VELFE YFISINEDLV LKCLKSLGLT GELFEIRLTT SLFTNADVNT DIVKQLSDPV ETTKKDTASF QTFLDKHSEL INTTN VSML TETGERYKKV LSLFTEAIGK GYKASSFLTS FFTTLESRIT FLLRVTISPA APTALKLISL NNIAKYINSI EKEVNI FTL VPCLICALRD ASIKVRTGVK KILSLIAKRP STKHYFLSDK LYGENVTIPM LNPKDSEAWL SGFLNEYVTE NYDISRI LT PKRNEKVFLM FWANQALLIP SPYAKTVLLD NLNKSPTYAS SYSSLFEEFI SHYLENRSSW EKSCIANKTN FEHFERSL V NLVSPKEKQS FMIDFVLSAL NSDYEQLANI AAERLISIFA SLNNAQKLKI VQNIVDSSSN VESSYDTVGV LQSLPLDSD IFVSILNQNS ISNEMDQTDF SKRRRRRSST SKNAFLKEEV SQLAELHLRK LTIILEALDK VRNVGSEKLL FTLLSLLSDL ETLDQDGGL PVLYAQETLI SCTLNTITYL KEHGCTELTN VRADILVSAI RNSASPQVQN KLLLVIGSLA TLSSEVILHS V MPIFTFMG AHSIRQDDEF TTKVVERTIL TVVPALIKNS KGNEKEEMEF LLLSFTTALQ HVPRHRRVKL FSTLIKTLDP VK ALGSFLF LIAQQYSSAL VNFKIGEARI LIEFIKALLV DLHVNEELSG LNDLLDIIKL LTSSKSSSEK KKSLESRVLF SNG VLNFSE SEFLTFMNNT FEFINKITEE TDQDYYDVRR NLRLKVYSVL LDETSDKKLI RNIREEFGTL LEGVLFFINS VELT FSCIT SQENEEASDS ETSLSDHTTE IKEILFKVLG NVLQILPVDE FVNAVLPLLS TSTNEDIRYH LTLVIGSKFE LEGSE AIPI VNNVMKVLLD RMPLESKSVV ISQVILNTMT ALVSKYGKKL EGSILTQALT LATEKVSSDM TEVKISSLAL ITNCVQ VLG VKSIAFYPKI VPPSIKLFDA SLADSSNPLK EQLQVAILLL FAGLIKRIPS FLMSNILDVL HVIYFSREVD SSIRLSV IS LIIENIDLKE VLKVLFRIWS TEIATSNDTV AVSLFLSTLE STVENIDKKS ATSQSPIFFK LLLSLFEFRS ISSFDNNT I SRIEASVHEI SNSYVLKMND KVFRPLFVIL VRWAFDGEGV TNAGITETER LLAFFKFFNK LQENLRGIIT SYFTYLLEP VDMLLKRFIS KDMENVNLRR LVINSLTSSL KFDRDEYWKS TSRFELISVS LVNQLSNIEN SIGKYLVKAI GALASNNSGV DEHNQILNK LIVEHMKASC SSNEKLWAIR AMKLIYSKIG ESWLVLLPQL VPVIAELLED DDEEIEREVR TGLVKVVENV L GEPFDRYL D

UniProtKB: U3 small nucleolar RNA-associated protein 10

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Macromolecule #10: Utp4

MacromoleculeName: Utp4 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae BY4741 (yeast)
Molecular weightTheoretical: 81.782867 KDa
SequenceString: MSSSLLSVLK EKSRSLKIRN KPVKMTSQER MIVHRCRFVD FTPATITSLA FSHKSNINKL TPSDLRLAIG RSNGNIEIWN PRNNWFQEM VIEGGKDRSI EGLCWSNVNG ESLRLFSIGG STVVTEWDLA TGLPLRNYDC NSGVIWSISI NDSQDKLSVG C DNGTVVLI ...String:
MSSSLLSVLK EKSRSLKIRN KPVKMTSQER MIVHRCRFVD FTPATITSLA FSHKSNINKL TPSDLRLAIG RSNGNIEIWN PRNNWFQEM VIEGGKDRSI EGLCWSNVNG ESLRLFSIGG STVVTEWDLA TGLPLRNYDC NSGVIWSISI NDSQDKLSVG C DNGTVVLI DISGGPGVLE HDTILMRQEA RVLTLAWKKD DFVIGGCSDG RIRIWSAQKN DENMGRLLHT MKVDKAKKES TL VWSVIYL PRTDQIASGD STGSIKFWDF QFATLNQSFK AHDADVLCLT TDTDNNYVFS AGVDRKIFQF SQNTNKSQKN NRW VNSSNR LLHGNDIRAI CAYQSKGADF LVSGGVEKTL VINSLTSFSN GNYRKMPTVE PYSKNVLVNK EQRLVVSWSE STVK IWTMG TDSSTEQNYK LVCKLTLKDD QNISTCSLSP DGQVLVVGRP STTKVFHLQP VGNKLKVTKL DNDLLLRTST KLVKF IDNS KIVICSCEDD VFIVDLESEE DEKPQEVELL EVTSTKSSIK VPYINRINHL EVDQNIAVIS RGCGVVDILD LKARIS KPL ARLNNFITAV HINTSRKSVV VITADNKIYE FNMNLNSEAE NEDSESVLTQ WSKNNTDNLP KEWKTLKENC VGIFSDI EN SSRLWFWGAT WISRIDFDVD FPINK(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) HFFFTDKYKP LLFVDLISSN ELAIIERNPL TFHSKQKAFI QPKLVF

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Macromolecule #11: Utp1

MacromoleculeName: Utp1 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae BY4741 (yeast)
Molecular weightTheoretical: 104.097039 KDa
SequenceString: MKSDFKFSNL LGTVYRQGNI TFSDDGKQLL SPVGNRVSVF DLINNKSFTF EYEHRKNIAA IDLNKQGTLL ISIDEDGRAI LVNFKARNV LHHFNFKEKC SAVKFSPDGR LFALASGRFL QIWKTPDVNK DRQFAPFVRH RVHAGHFQDI TSLTWSQDSR F ILTTSKDL ...String:
MKSDFKFSNL LGTVYRQGNI TFSDDGKQLL SPVGNRVSVF DLINNKSFTF EYEHRKNIAA IDLNKQGTLL ISIDEDGRAI LVNFKARNV LHHFNFKEKC SAVKFSPDGR LFALASGRFL QIWKTPDVNK DRQFAPFVRH RVHAGHFQDI TSLTWSQDSR F ILTTSKDL SAKIWSVDSE EKNLAATTFN GHRDYVMGAF FSHDQEKIYT VSKDGAVFVW EFTKRPSDDD DNESEDDDKQ EE VDISKYS WRITKKHFFY ANQAKVKCVT FHPATRLLAV GFTSGEFRLY DLPDFTLIQQ LSMGQNPVNT VSVNQTGEWL AFG SSKLGQ LLVYEWQSES YILKQQGHFD STNSLAYSPD GSRVVTASED GKIKVWDITS GFCLATFEEH TSSVTAVQFA KRGQ VMFSS SLDGTVRAWD LIRYRNFRTF TGTERIQFNC LAVDPSGEVV CAGSLDNFDI HVWSVQTGQL LDALSGHEGP VSCLS FSQE NSVLASASWD KTIRIWSIFG RSQQVEPIEV YSDVLALSMR PDGKEVAVST LKGQISIFNI EDAKQVGNID CRKDII SGR FNQDRFTAKN SERSKFFTTI HYSFDGMAIV AGGNNNSICL YDVPNEVLLK RFIVSRNMAL NGTLEFLNSK KMTEAGS LD LIDDAGENSD LEDRIDNSLP GSQRGGDLST RKMRPEVRVT SVQFSPTANA FAAASTEGLL IYSTNDTILF DPFDLDVD V TPHSTVEALR EKQFLNALVM AFRLNEEYLI NKVYEAIPIK EIPLVASNIP AIYLPRILKF IGDFAIESQH IEFNLIWIK ALLSASGGYI NEHKYLFSTA MRSIQRFIVR VAKEVVNTTT DNKYTYRFLV STDGSMEDGA ADDDEVLLKD DADEDNEENE ENDVVMESD DEEGWIGFNG KDNKLPLSNE NDSSDEEENE KELP

UniProtKB: Periodic tryptophan protein 2

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Macromolecule #12: Utp6

MacromoleculeName: Utp6 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae BY4741 (yeast)
Molecular weightTheoretical: 46.820391 KDa
SequenceString: MSKTRYYLEQ CIPEMDDLVE KGLFTKNEVS LIMKKRTDFE HRLNSRGSSI NDYIKYINYE SNVNKLRAKR CKRILQVKKT NSLSDWSIQ QRIGFIYQRG TNKFPQDLKF WAMYLNYMKA RGNQTSYKKI HNIYNQLLKL HPTNVDIWIS CAKYEYEVHA N FKSCRNIF ...String:
MSKTRYYLEQ CIPEMDDLVE KGLFTKNEVS LIMKKRTDFE HRLNSRGSSI NDYIKYINYE SNVNKLRAKR CKRILQVKKT NSLSDWSIQ QRIGFIYQRG TNKFPQDLKF WAMYLNYMKA RGNQTSYKKI HNIYNQLLKL HPTNVDIWIS CAKYEYEVHA N FKSCRNIF QNGLRFNPDV PKLWYEYVKF ELNFITKLIN RRKVMGLINE REQELDMQNE QKNNQAPDEE KSHLQVPSTG DS MKDKLNE LPEADISVLG NAETNPALRG DIALTIFDVC MKTLGKHYIN KHKGYYAISD SKMNIELNKE TLNYLFSESL RYI KLFDEF (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)

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Macromolecule #13: Utp12

MacromoleculeName: Utp12 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae BY4741 (yeast)
Molecular weightTheoretical: 102.690555 KDa
SequenceString: MVKSYQRFEQ AAAFGVIASN ANCVWIPASS GNSNGSGPGQ LITSALEDVN IWDIKTGDLV SKLSDGLPPG ASDARGAKPA ECTYLEAHK DTDLLAVGYA DGVIKVWDLM SKTVLLNFNG HKAAITLLQF DGTGTRLISG SKDSNIIVWD LVGEVGLYKL R SHKDSITG ...String:
MVKSYQRFEQ AAAFGVIASN ANCVWIPASS GNSNGSGPGQ LITSALEDVN IWDIKTGDLV SKLSDGLPPG ASDARGAKPA ECTYLEAHK DTDLLAVGYA DGVIKVWDLM SKTVLLNFNG HKAAITLLQF DGTGTRLISG SKDSNIIVWD LVGEVGLYKL R SHKDSITG FWCQGEDWLI STSKDGMIKL WDLKTHQCIE THIAHTGECW GLAVKDDLLI TTGTDSQVKI WKLDIENDKM GG KLTEMGI FEKQSKQRGL KIEFITNSSD KTSFFYIQNA DKTIETFRIR KEEEIARGLK KREKRLKE(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)Y SSFILHPFQT IRSLYKIKSA SWTTVSSSKL ELVLTTSSNT IEYY SIPYE KRDPTSPAPL KTHTIELQGQ RTDVRSIDIS DDNKLLATAS NGSLKIWNIK THKCIRTFEC GYALTCKFLP GGLLV ILGT RNGELQLFDL ASSSLLDTIE DAHDAAIWSL DLTSDGKRLV TGSADKTVKF WDFKVENSLV PGTKNKFLPV LKLHHD TTL ELTDDILCVR VSPDDRYLAI SLLDNTVKVF FLDSMKFYLS LYGHKLPVLS IDISFDSKMI ITSSADKNIK IWGLDFG DC HKSLFAHQDS IMNVKFLPQS HNFFSCSKDA VVKYWDGEKF ECIQKLYAHQ SEVWALAVAT DGGFVVSSSH DHSIRIWE E TED(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)S GVHKQTLESL KAGERLMEAL DLGIAEIEG LEAYNRDMKL WQRKKLGEAP IKPQGNAVLI AVNKTPEQYI MDTLLRIRMS QLEDALMVMP FSYVLKFLKF IDTVMQNKTL LHSHLPLIC KNLFFIIKFN HKELVSQKNE ELKLQINRVK TELRSALKST EDDLGFNVQG LKFVKQQWNL RHNYEFVDEY D QQEKESNS ARKRVFGTVI

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Macromolecule #14: Utp13

MacromoleculeName: Utp13 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae BY4741 (yeast)
Molecular weightTheoretical: 68.142312 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)EQEIEEEQ EKAKLQVEQE QSLQNYMSKG DWTNAFLLAM TLDHPMR LF NVLKRALGES RSRQDTEEGK IEVIFNEELD QAISILNDEQ LILLMKRCRD WNTNAKTHTI AQRTIRCILM HHNIAKLS E IPGMVKIVDA IIPYTQRHFT RVDNLVEQSY ILDYALVEMD KLF

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Macromolecule #15: Utp18

MacromoleculeName: Utp18 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae BY4741 (yeast)
Molecular weightTheoretical: 66.49425 KDa
SequenceString: MTMATTAMNV SVPPPDEEEQ LLAKFVFGDT TDLQENLAKF NADFIFNEQE MDVEDQEDEG SESDNSEEDE AQNGELDHVN NDQLFFVDD GGNEDSQDKN EDTMDVDDED DSSSDDYSED SEEAAWIDSD DEKIKVPILV TNKTKKLRTS YNESKINGVH Y INRLRSQF ...String:
MTMATTAMNV SVPPPDEEEQ LLAKFVFGDT TDLQENLAKF NADFIFNEQE MDVEDQEDEG SESDNSEEDE AQNGELDHVN NDQLFFVDD GGNEDSQDKN EDTMDVDDED DSSSDDYSED SEEAAWIDSD DEKIKVPILV TNKTKKLRTS YNESKINGVH Y INRLRSQF EKIYPRPKWV DDESDSELDD EEDDEEEGSN NVINGDINAL TKILSTTYNY KDTLSNSKLL PPKKLDIVRL KD ANASHPS HSAIQSLSFH PSKPLLLTGG YDKTLRIYHI DGKTNHLVTS LHLVGSPIQT CTFYTSLSNQ NQQNIFTAGR RRY MHSWDL SLENLTHSQT AKIEKFSRLY GHESTQRSFE NFKVAHLQNS QTNSVHGIVL LQGNNGWINI LHSTSGLWLM GCKI EGVIT DFCIDYQPIS RGKFRTILIA VNAYGEVWEF DLNKNGHVIR RWKDQGGVGI TKIQVGGGTT TTCPALQISK IKQNR WLAV GSESGFVNLY DRNNAMTSST PTPVAALDQL TTTISNLQFS PDGQILCMAS RAVKDALRLV HLPSCSVFSN WPTSGT PLG KVTSVAFSPS GGLLAVGNEQ GKVRLWKLNH Y

UniProtKB: U3 small nucleolar RNA-associated protein 18

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Macromolecule #16: Utp21

MacromoleculeName: Utp21 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae BY4741 (yeast)
Molecular weightTheoretical: 104.927844 KDa
SequenceString: MSIDLKKRKV EEDVRSRGKN SKIFSPFRII GNVSNGVPFA TGTLGSTFYI VTCVGKTFQI YDANTLHLLF VSEKETPSSI VALSAHFHY VYAAYENKVG IYKRGIEEHL LELETDANVE HLCIFGDYLC ASTDDNSIFI YKKSDPQDKY PSEFYTKLTV T EIQGGEIV ...String:
MSIDLKKRKV EEDVRSRGKN SKIFSPFRII GNVSNGVPFA TGTLGSTFYI VTCVGKTFQI YDANTLHLLF VSEKETPSSI VALSAHFHY VYAAYENKVG IYKRGIEEHL LELETDANVE HLCIFGDYLC ASTDDNSIFI YKKSDPQDKY PSEFYTKLTV T EIQGGEIV SLQHLATYLN KLTVVTKSNV LLFNVRTGKL VFTSNEFPDQ ITTAEPAPVL DIIALGTVTG EVIMFNMRKG KR IRTIKIP QSRISSLSFR TDGSSHLSVG TSSGDLIFYD LDRRSRIHVL KNIHRESYGG VTQATFLNGQ PIIVTSGGDN SLK EYVFDP SLSQGSGDVV VQPPRYLRSR GGHSQPPSYI AFADSQSHFM LSASKDRSLW SFSLRKDAQS QEMSQRLHKK QDGG RVGGS TIKSKFPEIV ALAIENARIG EWENIITAHK DEKFARTWDM RNKRVGRWTF DTTDDGFVKS VAMSQCGNFG FIGSS NGSI TIYNMQSGIL RKKYKLHKRA VTGISLDGMN RKMVSCGLDG IVGFYDFNKS TLLGKLKLDA PITAMVYHRS SDLFAL ALD DLSIVVIDAV TQRVVRQLWG HSNRITAFDF SPEGRWIVSA SLDSTIRTWD LPTGGCIDGI IVDNVATNVK FSPNGDL LA TTHVTGNGIC IWTNRAQFKT VSTRTIDESE FARMALPSTS VRGNDSMLSG ALESNGGEDL NDIDFNTYTS LEQIDKEL L TLSIGPRSKM NTLLHLDVIR KRSKPKEAPK KSEKLPFFLQ LSGEKVGDEA SVREGIAHET PEEIHRRDQE AQKKLDAEE QMNKFKVTGR LGFESHFTKQ LREGSQSKDY SSLLATLINF SPAAVDLEIR SLNSFEPFDE IVWFIDALTQ GLKSNKNFEL YETFMSLLF KAHGDVIHAN NKNQDIASAL QNWEDVHKKE DRLDDLVKFC MGVAAFVTTA

UniProtKB: U3 small nucleolar RNA-associated protein 21

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Macromolecule #17: Sof1

MacromoleculeName: Sof1 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae BY4741 (yeast)
Molecular weightTheoretical: 56.888918 KDa
SequenceString: MKIKTIKRSA DDYVPVKSTQ ESQMPRNLNP ELHPFERARE YTKALNATKL ERMFAKPFVG QLGYGHRDGV YAIAKNYGSL NKLATGSAD GVIKYWNMST REEFVSFKAH YGLVTGLCVT QPRFHDKKPD LKSQNFMLSC SDDKTVKLWS INVDDYSNKN S SDNDSVTN ...String:
MKIKTIKRSA DDYVPVKSTQ ESQMPRNLNP ELHPFERARE YTKALNATKL ERMFAKPFVG QLGYGHRDGV YAIAKNYGSL NKLATGSAD GVIKYWNMST REEFVSFKAH YGLVTGLCVT QPRFHDKKPD LKSQNFMLSC SDDKTVKLWS INVDDYSNKN S SDNDSVTN EEGLIRTFDG ESAFQGIDSH RENSTFATGG AKIHLWDVNR LKPVSDLSWG ADNITSLKFN QNETDILAST GS DNSIVLY DLRTNSPTQK IVQTMRTNAI CWNPMEAFNF VTANEDHNAY YYDMRNLSRS LNVFKDHVSA VMDVDFSPTG DEI VTGSYD KSIRIYKTNH GHSREIYHTK RMQHVFQVKY SMDSKYIISG SDDGNVRLWR SKAWERSNVK TTREKNKLEY DEKL KERFR HMPEIKRISR HRHVPQVIKK AQEIKNIELS SIKRREANER RTRKDMPYIS ERKKQIVGTV HKYEDSGRDR KRRKE DDKR DTQEK

UniProtKB: Protein SOF1

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Macromolecule #18: Utp7

MacromoleculeName: Utp7 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae BY4741 (yeast)
Molecular weightTheoretical: 62.41857 KDa
SequenceString: MGHKKNGHRR QIKERENQNK FERSTYTNNA KNNHTQTKDK KLRAGLKKID EQYKKAVSSA AATDYLLPES NGYLEPENEL EKTFKVQQS EIKSSVDVST ANKALDLSLK EFGPYHIKYA KNGTHLLITG RKGHVASMDW RKGQLRAELF LNETCHSATY L QNEQYFAV ...String:
MGHKKNGHRR QIKERENQNK FERSTYTNNA KNNHTQTKDK KLRAGLKKID EQYKKAVSSA AATDYLLPES NGYLEPENEL EKTFKVQQS EIKSSVDVST ANKALDLSLK EFGPYHIKYA KNGTHLLITG RKGHVASMDW RKGQLRAELF LNETCHSATY L QNEQYFAV AQKKYTFIYD HEGTELHRLK QHIEARHLDF LPYHYLLVTA GETGWLKYHD VSTGQLVSEL RTKAGPTMAM AQ NPWNAVM HLGHSNGTVS LWSPSMPEPL VKLLSARGPV NSIAIDRSGY YMATTGADRS MKIWDIRNFK QLHSVESLPT PGT NVSISD TGLLALSRGP HVTLWKDALK LSGDSKPCFG SMGGNPHRNT PYMSHLFAGN KVENLGFVPF EDLLGVGHQT GITN LIVPG AGEANYDALE LNPFETKKQR QEQEVRTLLN KLPADTITLD PNSIGSVDKR SSTIRLNAKD LAQTTMDANN KAKTN SDIP DVKPDVKGKN SGLRSFLRKK TQNVIDERKL RVQKQLDKEK NIRKRNHQIK QGLISEDHKD VIEEALSRFG

UniProtKB: U3 small nucleolar RNA-associated protein 7

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Macromolecule #19: Imp3

MacromoleculeName: Imp3 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae BY4741 (yeast)
Molecular weightTheoretical: 21.928529 KDa
SequenceString:
MVRKLKHHEQ KLLKKVDFLE WKQDQGHRDT QVMRTYHIQN REDYHKYNRI CGDIRRLANK LSLLPPTDPF RRKHEQLLLD KLYAMGVLT TKSKISDLEN KVTVSAICRR RLPVIMHRLK MAETIQDAVK FIEQGHVRVG PNLINDPAYL VTRNMEDYVT W VDNSKIKK TLLRYRNQID DFDFS

UniProtKB: U3 small nucleolar ribonucleoprotein protein IMP3

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Macromolecule #20: Mpp10

MacromoleculeName: Mpp10 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae BY4741 (yeast)
Molecular weightTheoretical: 67.042492 KDa
SequenceString: MSELFGVLKS NAGRIILKDP SATSKDVKAY IDSVINTCKN GSITKKAELD EITVDGLDAN QVWWQVKLVL DSIDGDLIQG IQELKDVVT PSHNLSDGST LNSSSGEESE LEEAESVFKE KQMLSADVSE IEEQSNDSLS ENDEEPSMDD EKTSAEAARE E FAEEKRIS ...String:
MSELFGVLKS NAGRIILKDP SATSKDVKAY IDSVINTCKN GSITKKAELD EITVDGLDAN QVWWQVKLVL DSIDGDLIQG IQELKDVVT PSHNLSDGST LNSSSGEESE LEEAESVFKE KQMLSADVSE IEEQSNDSLS ENDEEPSMDD EKTSAEAARE E FAEEKRIS SGQDERHSSP DPYGINDKFF DLEKFNRDTL AAEDSNEASE GSEDEDIDYF QDMPSDDEEE EAIYYEDFFD KP TKEPVKK HSDVKDPKED EELDEEEHDS AMDKVKLDLF ADEEDEPNAE GVGEASDKNL SSFEKQQIEI RKQIEQLENE AVA EKKWSL KGEVKAKDRP EDALLTEELE FDRTAKPVPV ITSEVTESLE DMIRRRIQDS NFDDLQRRTL LDITRKSQRP QFEL SDVKS SKSLAEIYED DYTRAEDESA LSEELQKAHS EISELYANLV YKLDVLSSVH FVPKPASTSL EIRVETPTIS MEDAQ PLYM SNASSLAPQE IYNVGKAEKD GEIRLKNGVA MSKEELTRED KNRLRRALKR KRSKANLPNV NKRSKRNDVV DTLSKA KNI TVINQKGEKK DVSGKTKKSR SGPDSTNIKL

UniProtKB: U3 small nucleolar RNA-associated protein MPP10

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Macromolecule #21: Bud21

MacromoleculeName: Bud21 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae BY4741 (yeast)
Molecular weightTheoretical: 24.431016 KDa
SequenceString: MSNGHVKFDA DESQASASAV TDRQDDVLVI SKKDKEVHSS SDEESDDDDA PQEEGLHSGK SEVESQITQR EEAIRLEQSQ LRSKRRKQN ELYAKQKKSV NETEVTDEVI AELPEELLKN IDQKDEGSTQ YSSSRHVTFD KLDESDENEE ALAKAIKTKK R KTLKNLRK ...String:
MSNGHVKFDA DESQASASAV TDRQDDVLVI SKKDKEVHSS SDEESDDDDA PQEEGLHSGK SEVESQITQR EEAIRLEQSQ LRSKRRKQN ELYAKQKKSV NETEVTDEVI AELPEELLKN IDQKDEGSTQ YSSSRHVTFD KLDESDENEE ALAKAIKTKK R KTLKNLRK DSVKRGKFRV QLLSTTQDSK TLPPKKESSI IRSKDRWLNR KALNKG

UniProtKB: U3 small nucleolar RNA-associated protein 16

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Macromolecule #22: Nop56

MacromoleculeName: Nop56 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae BY4741 (yeast)
Molecular weightTheoretical: 55.307637 KDa
SequenceString: MAPIEYLLFE EPTGYAVFKV KLQQDDIGSR LKEVQEQIND FGAFTKLIEL VSFAPFKGAA EALENANDIS EGLVSESLKA ILDLNLPKA SSKKKNITLA ISDKNLGPSI KEEFPYVDCI SNELAQDLIR GVRLHGEKLF KGLQSGDLER AQLGLGHAYS R AKVKFSVQ ...String:
MAPIEYLLFE EPTGYAVFKV KLQQDDIGSR LKEVQEQIND FGAFTKLIEL VSFAPFKGAA EALENANDIS EGLVSESLKA ILDLNLPKA SSKKKNITLA ISDKNLGPSI KEEFPYVDCI SNELAQDLIR GVRLHGEKLF KGLQSGDLER AQLGLGHAYS R AKVKFSVQ KNDNHIIQAI ALLDQLDKDI NTFAMRVKEW YGWHFPELAK LVPDNYTFAK LVLFIKDKA(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)SET DMENVCVFAQ RVASLADYRR QLYDYLCEKM HTVAPNL SE LIGEVIGARL ISHAGSLTNL SKQAASTVQI LGAEKALFRA LKTKGNTPKY GLIYHSGFIS KASAKNKGRI SRYLANKC S MASRIDNYSE EPSNVFGSVL KKQVEQRLEF YNTGKPTLKN ELAIQEAMEL YNKDKPAAEV EETKEKESSK KRKLEDDDE EKKEKKEKKS KKEKKEKKEK KDKKEKKDKK EKKDKKKKSK D

+
Macromolecule #23: Nop58

MacromoleculeName: Nop58 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae BY4741 (yeast)
Molecular weightTheoretical: 53.13368 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)DNDLSKMSL GLAHSIGRHK L KFSADKVD VMIIQAIALL DDLDKELNTY AMRCKEWYGW HFPELAKIVT DSVAYARIIL TMGIRSKASE TDLSEILPEE IE ERVKTAA EVSMGTEITQ TDLDNINALA EQIVEFAAYR EQLSNYLSAR MKAIAPNLTQ LVGELVGARL IAHSGSLISL AKS PASTIQ ILGAEKALFR ALKTKHDTPK YGLLYHASLV GQATGKNKGK IARVLAAKAA VSLRYDALAE DRDDSGDIGL ESRA KVENR LSQLEGRDLR TTPKVVREAK KVEMTEARAY NADADTAKAA SDSESDSDDE EEEKKEKKEK KRKRDDDEDS KDSKK AKKE KKDKKEKKEK KEKKEKKEKK EKKEKKSKKE KKEKK

+
Macromolecule #24: Nop1.1

MacromoleculeName: Nop1.1 / type: protein_or_peptide / ID: 24 / Number of copies: 2 / Enantiomer: LEVO
EC number: Transferases; Transferring one-carbon groups; Methyltransferases
Source (natural)Organism: Saccharomyces cerevisiae BY4741 (yeast)
Molecular weightTheoretical: 34.525418 KDa
SequenceString: MSFRPGSRGG SRGGSRGGFG GRGGSRGGAR GGSRGGFGGR GGSRGGARGG SRGGFGGRGG SRGGARGGSR GGRGGAAGGA RGGAKVVIE PHRHAGVYIA RGKEDLLVTK NMAPGESVYG EKRISVEEPS KEDGVPPTKV EYRVWNPFRS KLAAGIMGGL D ELFIAPGK ...String:
MSFRPGSRGG SRGGSRGGFG GRGGSRGGAR GGSRGGFGGR GGSRGGARGG SRGGFGGRGG SRGGARGGSR GGRGGAAGGA RGGAKVVIE PHRHAGVYIA RGKEDLLVTK NMAPGESVYG EKRISVEEPS KEDGVPPTKV EYRVWNPFRS KLAAGIMGGL D ELFIAPGK KVLYLGAASG TSVSHVSDVV GPEGVVYAVE FSHRPGRELI SMAKKRPNII PIIEDARHPQ KYRMLIGMVD CV FADVAQP DQARIIALNS HMFLKDQGGV VISIKANCID STVDAETVFA REVQKLREER IKPLEQLTLE PYERDHCIVV GRY MRSGLK K

UniProtKB: rRNA 2'-O-methyltransferase fibrillarin

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Macromolecule #25: Snu13

MacromoleculeName: Snu13 / type: protein_or_peptide / ID: 25 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae BY4741 (yeast)
Molecular weightTheoretical: 13.582855 KDa
SequenceString:
MSAPNPKAFP LADAALTQQI LDVVQQAANL RQLKKGANEA TKTLNRGISE FIIMAADCEP IEILLHLPLL CEDKNVPYVF VPSRVALGR ACGVSRPVIA ASITTNDASA IKTQIYAVKD KIETLLI

UniProtKB: 13 kDa ribonucleoprotein-associated protein

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Macromolecule #26: Rrp9

MacromoleculeName: Rrp9 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae BY4741 (yeast)
Molecular weightTheoretical: 65.146969 KDa
SequenceString: MSDVTQQKKR KRSKGEVNPS KPTVDEEITD PSSNEDEQLE VSDEEDALES EEEFEGENPA DKRRRLAKQY LENLKSEAND ILTDNRNAE EKDLNNLKER TIDEYNNFDA GDLDKDIIAS RLKEDVAEQQ GRVFRYFGDK LLISEAKQSF TRVGENNLTC I SCFQPVLN ...String:
MSDVTQQKKR KRSKGEVNPS KPTVDEEITD PSSNEDEQLE VSDEEDALES EEEFEGENPA DKRRRLAKQY LENLKSEAND ILTDNRNAE EKDLNNLKER TIDEYNNFDA GDLDKDIIAS RLKEDVAEQQ GRVFRYFGDK LLISEAKQSF TRVGENNLTC I SCFQPVLN KYTFEESSNG DKNKGRLFAY TVSKDLQLTK YDITDFSKRP KKLKYAKGGA KYIPTSKHEY ENTTEGHYDE IL TVAASPD GKYVVTGGRD RKLIVWSTES LSPVKVIPTK DRRGEVLSLA FRKNSDQLYA SCADFKIRTY SINQFSQLEI LYG HHDIVE DISALAMERC VTVGARDRTA MLWKIPDETR LTFRGGDEPQ KLLRRWMKEN AKEGEDGEVK YPDESEAPLF FCEG SIDVV SMVDDFHFIT GSDNGNICLW SLAKKKPIFT ERIAHGILPE PSFNDISGET DEELRKRQLQ GKKLLQPFWI TSLYA IPYS NVFISGSWSG SLKVWKISDN LRSFELLGEL SGAKGVVTKI QVVESGKHGK EKFRILASIA KEHRLGRWIA NVSGAR NGI YSAVIDQTGF

UniProtKB: Ribosomal RNA-processing protein 9

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Macromolecule #27: Utp24

MacromoleculeName: Utp24 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae BY4741 (yeast)
Molecular weightTheoretical: 21.650729 KDa
SequenceString:
MGKAKKTRKF GLVKRTLNTK KDQRLKKNQE NIKTKEDPEL TRNIPQVSSA LFFQYNQAIK PPYQVLIDTN FINFSIQKKV DIVRGMMDC LLAKCNPLIT DCVMAELEKL GPKYRIALKL ARDPRIKRLS CSHKGTYADD CLVHRVLQHK CYIVATNDAG L KQRIRKIP GIPLMSVGGH AYVIEKLPDV F

UniProtKB: rRNA-processing protein FCF1

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Macromolecule #28: Unidentified fragment

MacromoleculeName: Unidentified fragment / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae BY4741 (yeast)
Molecular weightTheoretical: 2.060531 KDa
SequenceString:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.7
Component:
ConcentrationFormulaName
50.0 mM(HOCH2)3CNH2Tris
150.0 mMNaClsodium chloride
5.0 mMMgCl2magnesium chloride
5.0 mMbiotin
5.0 mMputrescine
1.0 mMspermidine
0.03 %Triton X-100
GridSupport film - #0 - Film type ID: 1 / Support film - #0 - Material: CARBON / Support film - #0 - topology: LACEY / Support film - #1 - Film type ID: 2 / Support film - #1 - Material: CARBON / Support film - #1 - topology: CONTINUOUS / Support film - #1 - Film thickness: 2
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 295 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Digitization - Frames/image: 1-32 / Number grids imaged: 1 / Number real images: 2750 / Average exposure time: 0.25 sec. / Average electron dose: 31.25 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 3.5 µm / Nominal defocus min: 1.0 µm
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 275080
Startup modelType of model: OTHER / Details: CryoSparc initial model
Final reconstructionNumber classes used: 1 / Applied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 4.3 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3) / Number images used: 52629
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 2.1)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3)
Final 3D classificationNumber classes: 7 / Software - Name: RELION (ver. 3)
FSC plot (resolution estimation)

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Atomic model buiding 1

Initial modelPDB ID:

5wlc


Chain - Source name: PDB / Chain - Initial model type: experimental model
RefinementSpace: REAL / Protocol: OTHER
Output model

PDB-6nd4:
Conformational switches control early maturation of the eukaryotic small ribosomal subunit

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