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- EMDB-21530: Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-I... -

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Basic information

Entry
Database: EMDB / ID: EMD-21530
TitleStructure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the closed state (Class 2)
Map dataSharpened map after post processing for Class 2 (closed)
Sample
  • Complex: Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the closed state (Class 2)
    • RNA: x 2 types
    • Protein or peptide: x 42 types
  • Ligand: x 2 types
KeywordsCrPV 5'-UTR IRES / Internal ribosome entry site / RIBOSOME
Function / homology
Function and homology information


viral translational termination-reinitiation / eukaryotic translation initiation factor 3 complex, eIF3e / cap-dependent translational initiation / eukaryotic translation initiation factor 3 complex, eIF3m / IRES-dependent viral translational initiation / eukaryotic translation initiation factor 3 complex / eukaryotic 43S preinitiation complex / mRNA cap binding / formation of cytoplasmic translation initiation complex / eukaryotic 48S preinitiation complex ...viral translational termination-reinitiation / eukaryotic translation initiation factor 3 complex, eIF3e / cap-dependent translational initiation / eukaryotic translation initiation factor 3 complex, eIF3m / IRES-dependent viral translational initiation / eukaryotic translation initiation factor 3 complex / eukaryotic 43S preinitiation complex / mRNA cap binding / formation of cytoplasmic translation initiation complex / eukaryotic 48S preinitiation complex / ribosomal subunit / metal-dependent deubiquitinase activity / regulation of translational initiation / nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / translation initiation factor binding / translation initiation factor activity / DNA-(apurinic or apyrimidinic site) lyase / positive regulation of translation / PML body / fibrillar center / metallopeptidase activity / ribosome binding / cysteine-type deubiquitinase activity / postsynaptic density / structural constituent of ribosome / translation / synapse / nucleolus / RNA binding / nucleoplasm / identical protein binding / nucleus / cytosol
Similarity search - Function
Eukaryotic translation initiation factor 3 subunit D / Eukaryotic translation initiation factor 3 subunit 7 (eIF-3) / Eukaryotic translation initiation factor 3 subunit H / eIF3h, C-terminal / C-terminal region of eIF3h / Eukaryotic translation initiation factor 3 subunit F / Translation initiation factor 3 complex subunit L / RNA polymerase I-associated factor PAF67 / Eukaryotic translation initiation factor 3 subunit M / eIF3 subunit M, C-terminal helix domain ...Eukaryotic translation initiation factor 3 subunit D / Eukaryotic translation initiation factor 3 subunit 7 (eIF-3) / Eukaryotic translation initiation factor 3 subunit H / eIF3h, C-terminal / C-terminal region of eIF3h / Eukaryotic translation initiation factor 3 subunit F / Translation initiation factor 3 complex subunit L / RNA polymerase I-associated factor PAF67 / Eukaryotic translation initiation factor 3 subunit M / eIF3 subunit M, C-terminal helix domain / eIF3 subunit M, C-terminal helix / Eukaryotic translation initiation factor 3 subunit E, C-terminal / Eukaryotic translation initiation factor 3 subunit E / Eukaryotic translation initiation factor 3 subunit E, N-terminal / eIF3 subunit 6 N terminal domain / eIF3 subunit 6 N terminal domain / Eukaryotic translation initiation factor 3 subunit K / Translation initiation factor 3, subunit 12, N-terminal, eukaryotic / Eukaryotic translation initiation factor 3 subunit M eIF3m/COP9 signalosome complex subunit 7 COPS7 / Eukaryotic translation initiation factor 3 subunit C, N-terminal domain / Eukaryotic translation initiation factor 3 subunit C / Eukaryotic translation initiation factor 3 subunit 8 N-terminus / Eukaryotic translation initiation factor 3 subunit A / CSN8/PSMD8/EIF3K / CSN8/PSMD8/EIF3K family / Rpn11/EIF3F, C-terminal / Maintenance of mitochondrial structure and function / motif in proteasome subunits, Int-6, Nip-1 and TRIP-15 / PCI domain / Proteasome component (PCI) domain / PCI domain profile. / JAB1/Mov34/MPN/PAD-1 ubiquitin protease / Ribosomal protein S26e signature. / Ribosomal protein S12e signature. / Ribosomal protein S19e signature. / 40S Ribosomal protein S10 / Plectin/S10, N-terminal / Plectin/S10 domain / Ribosomal protein S17e signature. / Ribosomal protein S7e signature. / Ribosomal protein S3Ae signature. / Ribosomal protein S27e signature. / Ribosomal protein S4e signature. / Ribosomal protein S8e signature. / Ribosomal S24e conserved site / Ribosomal protein S24e signature. / Ribosomal protein S24e / Ribosomal protein S24e / Ribosomal protein S6e signature. / Ribosomal protein S28e signature. / JAB/MPN domain / JAB1/MPN/MOV34 metalloenzyme domain / MPN domain / MPN domain profile. / Ribosomal protein S2 signature 2. / Ubiquitin domain signature. / Ribosomal protein S3 signature. / Ribosomal protein S10 signature. / Ribosomal protein S14 signature. / Ribosomal protein S2 signature 1. / Type-2 KH domain profile. / Ribosomal protein S19 signature. / Ribosomal protein S5 signature. / Ribosomal protein S7 signature. / S5 double stranded RNA-binding domain profile. / Ribosomal protein S17 signature. / Ribosomal protein S13 signature. / Ribosomal protein S13 family profile. / Ribosomal protein S8 signature. / Ribosomal protein S4 signature. / Ribosomal protein S15 signature. / S4 RNA-binding domain profile. / Ribosomal protein S11 signature. / Ubiquitin domain profile. / Ribosomal protein S9 signature. / Tetratricopeptide-like helical domain superfamily / Ribosomal protein S12 signature. / Ribosomal protein L23/L15e core domain superfamily / Armadillo-type fold / Winged helix DNA-binding domain superfamily / Trp-Asp (WD) repeats signature. / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / Winged helix-like DNA-binding domain superfamily
Similarity search - Domain/homology
Small ribosomal subunit protein uS4 / Eukaryotic translation initiation factor 3 subunit L / Small ribosomal subunit protein eS12 / Small ribosomal subunit protein uS9 / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein RACK1 / Ubiquitin-ribosomal protein eS31 fusion protein / Eukaryotic translation initiation factor 3 subunit F / Eukaryotic translation initiation factor 3 subunit M / Eukaryotic translation initiation factor 3 subunit A ...Small ribosomal subunit protein uS4 / Eukaryotic translation initiation factor 3 subunit L / Small ribosomal subunit protein eS12 / Small ribosomal subunit protein uS9 / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein RACK1 / Ubiquitin-ribosomal protein eS31 fusion protein / Eukaryotic translation initiation factor 3 subunit F / Eukaryotic translation initiation factor 3 subunit M / Eukaryotic translation initiation factor 3 subunit A / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein eS1 / Eukaryotic translation initiation factor 3 subunit H / Eukaryotic translation initiation factor 3 subunit E / Small ribosomal subunit protein eS7 / Small ribosomal subunit protein uS5 / Small ribosomal subunit protein uS12 / 40S ribosomal protein S24 / Eukaryotic translation initiation factor 3 subunit K / Ubiquitin-like FUBI-ribosomal protein eS30 fusion protein / Small ribosomal subunit protein eS25 / Small ribosomal subunit protein eS26 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein eS28 / Small ribosomal subunit protein eS8 / Small ribosomal subunit protein eS4 / Small ribosomal subunit protein eS6 / Small ribosomal subunit protein eS19 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS13 / Plectin/eS10 N-terminal domain-containing protein / Small ribosomal subunit protein uS17 / Small ribosomal subunit protein eS17 / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein eS27 / Small ribosomal subunit protein uS19 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein uS14 / Eukaryotic translation initiation factor 3 subunit C / Eukaryotic translation initiation factor 3 subunit D
Similarity search - Component
Biological speciesOryctolagus cuniculus (rabbit) / Cricket paralysis virus
Methodsingle particle reconstruction / cryo EM / Resolution: 3.36 Å
AuthorsNeupane R / Pisareva V
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM097014 United States
CitationJournal: Elife / Year: 2020
Title: A complex IRES at the 5'-UTR of a viral mRNA assembles a functional 48S complex via an uAUG intermediate.
Authors: Ritam Neupane / Vera P Pisareva / Carlos F Rodriguez / Andrey V Pisarev / Israel S Fernández /
Abstract: Taking control of the cellular apparatus for protein production is a requirement for virus progression. To ensure this control, diverse strategies of cellular mimicry and/or ribosome hijacking have ...Taking control of the cellular apparatus for protein production is a requirement for virus progression. To ensure this control, diverse strategies of cellular mimicry and/or ribosome hijacking have evolved. The initiation stage of translation is especially targeted as it involves multiple steps and the engagement of numerous initiation factors. The use of structured RNA sequences, called nternal ibosomal ntry ites (IRES), in viral RNAs is a widespread strategy for the exploitation of eukaryotic initiation. Using a combination of electron cryo-microscopy (cryo-EM) and reconstituted translation initiation assays with native components, we characterized how a novel IRES at the 5'-UTR of a viral RNA assembles a functional initiation complex via an uAUG intermediate. The IRES features a novel extended, multi-domain architecture, that circles the 40S head. The structures and accompanying functional data illustrate the importance of 5'-UTR regions in translation regulation and underline the relevance of the untapped diversity of viral IRESs.
History
DepositionMar 8, 2020-
Header (metadata) releaseApr 22, 2020-
Map releaseApr 22, 2020-
UpdateMar 20, 2024-
Current statusMar 20, 2024Processing site: RCSB / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.018
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by height
  • Surface level: 0.018
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6w2t
  • Surface level: 0.018
  • Imaged by UCSF Chimera
  • Download
Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_21530.png

Downloads & links

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Map

FileDownload / File: emd_21530.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationSharpened map after post processing for Class 2 (closed)
Voxel sizeX=Y=Z: 1.0605 Å
Density
Contour LevelBy AUTHOR: 0.018 / Movie #1: 0.018
Minimum - Maximum-0.10054721 - 0.1714331
Average (Standard dev.)0.00037778862 (±0.0043628775)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions400400400
Spacing400400400
CellA=B=C: 424.2 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.06051.06051.0605
M x/y/z400400400
origin x/y/z0.0000.0000.000
length x/y/z424.200424.200424.200
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS400400400
D min/max/mean-0.1010.1710.000

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Supplemental data

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Mask #1

Fileemd_21530_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: Unsharpened map before post-processing for Class 2 (closed)

Fileemd_21530_additional.map
AnnotationUnsharpened map before post-processing for Class 2 (closed)
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half map 2 for Class 2 (closed)

Fileemd_21530_half_map_1.map
AnnotationHalf map 2 for Class 2 (closed)
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half map 1 for Class 2 (closed)

Fileemd_21530_half_map_2.map
AnnotationHalf map 1 for Class 2 (closed)
Projections & Slices
AxesZYX

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Slices (1/2)
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Sample components

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Entire : Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-I...

EntireName: Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the closed state (Class 2)
Components
  • Complex: Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the closed state (Class 2)
    • RNA: 18S rRNA18S ribosomal RNA
    • Protein or peptide: uS2
    • Protein or peptide: eS1
    • Protein or peptide: uS5
    • Protein or peptide: eS4
    • Protein or peptide: eS6
    • Protein or peptide: eS7
    • Protein or peptide: eS8
    • Protein or peptide: uS4
    • Protein or peptide: uS17
    • Protein or peptide: uS15
    • Protein or peptide: uS11
    • Protein or peptide: eS21
    • Protein or peptide: uS8
    • Protein or peptide: uS12
    • Protein or peptide: eS24
    • Protein or peptide: eS26
    • Protein or peptide: eS27
    • Protein or peptide: eS30
    • Protein or peptide: uS3
    • Protein or peptide: uS7
    • Protein or peptide: eS10
    • Protein or peptide: eS12
    • Protein or peptide: uS19
    • Protein or peptide: uS9
    • Protein or peptide: eS17
    • Protein or peptide: uS13
    • Protein or peptide: eS19
    • Protein or peptide: uS10
    • Protein or peptide: eS25
    • Protein or peptide: eS28
    • Protein or peptide: eS29
    • Protein or peptide: eS31
    • Protein or peptide: RACK1Receptor for activated C kinase 1
    • Protein or peptide: Eukaryotic translation initiation factor 3 subunit EEukaryotic initiation factor 3
    • Protein or peptide: Eukaryotic translation initiation factor 3 subunit FEukaryotic initiation factor 3
    • Protein or peptide: Eukaryotic translation initiation factor 3 subunit KEukaryotic initiation factor 3
    • Protein or peptide: Eukaryotic translation initiation factor 3 subunit LEukaryotic initiation factor 3
    • Protein or peptide: Eukaryotic translation initiation factor 3 subunit MEukaryotic initiation factor 3
    • Protein or peptide: Eukaryotic translation initiation factor 3 subunit AEukaryotic initiation factor 3
    • Protein or peptide: Eukaryotic translation initiation factor 3 subunit CEukaryotic initiation factor 3
    • Protein or peptide: Eukaryotic translation initiation factor 3 subunit DEukaryotic initiation factor 3
    • Protein or peptide: Eukaryotic translation initiation factor 3 subunit HEukaryotic initiation factor 3
    • RNA: CrPV 5'-UTR IRES
  • Ligand: MAGNESIUM ION
  • Ligand: ZINC ION

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Supramolecule #1: Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-I...

SupramoleculeName: Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the closed state (Class 2)
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#44
Source (natural)Organism: Oryctolagus cuniculus (rabbit)

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Macromolecule #1: 18S rRNA

MacromoleculeName: 18S rRNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 547.733062 KDa
SequenceString: UACCUGGUUG AUCCUGCCAG UAGCAUAUGC UUGUCUCAAA GAUUAAGCCA UGCAUGUCUA AGUACGCACG GCCGGUACAG UGAAACUGC GAAUGGCUCA UUAAAUCAGU UAUGGUUCCU UUGGUCGCUC GACUUGGAUA ACUGUGGUAA UUCUAGAGCU A AUACAUGC ...String:
UACCUGGUUG AUCCUGCCAG UAGCAUAUGC UUGUCUCAAA GAUUAAGCCA UGCAUGUCUA AGUACGCACG GCCGGUACAG UGAAACUGC GAAUGGCUCA UUAAAUCAGU UAUGGUUCCU UUGGUCGCUC GACUUGGAUA ACUGUGGUAA UUCUAGAGCU A AUACAUGC CGACGGGCGC UGACCCCCUU CGCGGGGGGG AUGCGUGCAU UUAUCAGUGG UGACUCUAGA UAACCUCGGG CC GAUCGCA CGCCCGGCGG CGACGACCCA UUCGAACGUC UGCCCUAUCA ACUUUCGAUG GUAGUCGCCG UGCCUACCAU GGU GACCAC GGGUGACGGG GAAUCAGGGU UCGAUUCCGG AGAGGGAGCC UGAGAAACGG CUACCACAUC CAAGGAAGGC AGCA GGCGC GCAAAUUACC CACUCCCGAC CCGGGGAGGU AGUGACGAAA AAUAACAAUA CAGGACUCUU UCGAGGCCCU GUAAU UGGA AUGAGUCCAC UUUAAAUCCU UUAACGAGGA UCCAUUGGAG GGCAAGUCUG GUGCCAGCAG CCGCGGUAAU UCCAGC UCC AAUAGCGUAU AUUAAAGUUG CUGCAGUUAA AAAGCUCGUA GUUGGAUCUU GGGAGCGGCC GUCCCCUGCU CGGCGCC GG CCCGAAGCGU UUACUUUGAA AAAAUUAGAG UGUUCAAAGC AGGCCGCCUG GAUACCGCAG CUAGGAAUAA UGGAAUAG G ACCGCGGUUC UAUUUUGUUG GUUUUCGGAA CUGAGGCCAU GAUUAAGAGG GACGGCCGGG GGCAUUCGUA UUGCGCCGC UAGAGGUGAA AUUCUUGGAC CGGCGCAAGA CGGACCAGAG CGAAAGCAUU UGCCAAGAAU GUUUUCAUUA AUCAAGAACG AAAGUCGGA GGUUCGAAGA CGAUCAGAUA CCGUCGUAGU UCCGACCAUA AACGAUGCCG ACCGGCGAUG CGGCGGCGUU A UUCCCAUG ACCCGCCGGG CAGCUUCCGG GAAACCAAAG UCUUUGGGUU CCGGGGGGAG UAUGGUUGCA AAGCUGAAAC UU AAAGGAA UUGACGGAAG GGCACCACCA GGAGUGGAGC CUGCGGCUUA AUUUGACUCA ACACGGGAAA CCUCACCCGG CCC GGACAC GGACAGGAUU GACAGAUUGA UAGCUCUUUC UCGAUUCCGU GGGUGGUGGU GCAUGGCCGU UCUUAGUUGG UGGA GCGAU UUGUCUGGUU AAUUCCGAUA ACGAACGAGA CUCUGGCAUG CUAACUAGUU ACGCGACCCC GGUCGGCGUA ACUUC UUAG AGGGACAAGU GGCGUUCAGC CACCCGAGAU UGAGCAAUAA CAGGUCUGUG AUGCCCUUAG AUGUCCGGGG CUGCAC GCG CGCUACACUG ACUGGCUCAG CGUGUGCCUA CCCUACGCCG GCAGGCGCGG GUAACCCGUU GAACCCCAUU CGUGAUG GG GAUCGGGGAU UGCAAUUAUU CCCCAUGAAC GAGGAAUUCC CAGUAAGUGC GGGUCAUAAG CUUGCGUUGA UUAAGUCC C UGCCCUUUGU ACACACCGCC CGUCGCUACU ACCGAUUGGA UGGUUUAGUG AGGCCCUCGG AUCGGCCCCG CCGGGGUGC CCUGGCGGAG CGCUGAGAAG ACGGUCGAAC UUGACUAUCU AGAGGAAGUA AAAGUCGUAA CAAGGUUUCC GUAGGUGAAC CUGCGGAAG GAUCAUUA

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Macromolecule #44: CrPV 5'-UTR IRES

MacromoleculeName: CrPV 5'-UTR IRES / type: rna / ID: 44
Details: Positions 211 to 220 are only modeled as a polyuridine stretch.
Number of copies: 1
Source (natural)Organism: Cricket paralysis virus
Molecular weightTheoretical: 121.094953 KDa
SequenceString: UUCGAUACCA AGAGCUGGUG UCUGAGGGAU UACGACUCGC GUGGUCGGAC AGCACAUGUU AGUCAUGUAU UAUGGUGUUU CCCAACACG UAUCUUUGCU CUAGUAUUGA ACUUUAGUGA UGAGUGUACU UGGCAUGUAC ACCUGAUUAG UUAGCCCUUG U GGAUACGC ...String:
UUCGAUACCA AGAGCUGGUG UCUGAGGGAU UACGACUCGC GUGGUCGGAC AGCACAUGUU AGUCAUGUAU UAUGGUGUUU CCCAACACG UAUCUUUGCU CUAGUAUUGA ACUUUAGUGA UGAGUGUACU UGGCAUGUAC ACCUGAUUAG UUAGCCCUUG U GGAUACGC CACUGCCGCU CAGGUACAGU AGGACAUACC CCAAAUAUUG GGUUUUUUUU UUAGAGAAGA AAAUUAGUGG CG AAACGUA CGGUUUAUGU CAAAGGAGAA GGUGAUUUUA AUUGCACCUG AAGCAACCCU AGGUCUUCCC UUUGAUGUGA GCU AAUAAG GAGUAACCGA UUCUUACAAU GUGAUCAUGU CUUUUCAACA AACAAAGGAU C

GENBANK: GENBANK: AF218039.1

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Macromolecule #2: uS2

MacromoleculeName: uS2 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 33.022055 KDa
SequenceString: MSGALDVLQM KEEDVLKFLA AGTHLGGTNL DFQMEQYIYK RKSDGIYIIN LKRTWEKLLL AARAIVAIEN PADVSVISSR NTGQRAVLK FAAATGATPI AGRFTPGTFT NQIQTAFREP RLLVVTDPQA DHQPLTEASY VNLPTIALCN TDSPLRYVDI A IPCNNKGA ...String:
MSGALDVLQM KEEDVLKFLA AGTHLGGTNL DFQMEQYIYK RKSDGIYIIN LKRTWEKLLL AARAIVAIEN PADVSVISSR NTGQRAVLK FAAATGATPI AGRFTPGTFT NQIQTAFREP RLLVVTDPQA DHQPLTEASY VNLPTIALCN TDSPLRYVDI A IPCNNKGA HSVGLMWWML AQEVLRMRGT ISREHPWEVM PDLYFYRDPE EIEKEEQAAA EKAVTKEEFQ GEWTAPEPEF TA TQPEVAD WSEGMQVPSV PIQQFPTEDW SAQPATEDWS AAPTAQATEW MGTTTEWS

UniProtKB: Small ribosomal subunit protein uS2

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Macromolecule #3: eS1

MacromoleculeName: eS1 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 30.002061 KDa
SequenceString: MAVGKNKRLT KGGKKGAKKK VVDPFSKKDW YDVKAPAMFN IRNIGKTLVT RTQGTKIASD GLKGRVFEVS LADLQNDEVA FRKFKLITE DVQGKNCLTN FHGMDLTRDK MCSMVKKWQT MIEAHVDVKT TDGYLLRLFC VGFTKKRNNQ IRKTSYAQHQ Q VRQIRKKM ...String:
MAVGKNKRLT KGGKKGAKKK VVDPFSKKDW YDVKAPAMFN IRNIGKTLVT RTQGTKIASD GLKGRVFEVS LADLQNDEVA FRKFKLITE DVQGKNCLTN FHGMDLTRDK MCSMVKKWQT MIEAHVDVKT TDGYLLRLFC VGFTKKRNNQ IRKTSYAQHQ Q VRQIRKKM MEIMTREVQT NDLKEVVNKL IPDSIGKDIE KACQSIYPLH DVFVRKVKML KKPKFELGKL MELHGEGSSS GK ATGDETG AKVERADGYE PPVQESV

UniProtKB: Small ribosomal subunit protein eS1

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Macromolecule #4: uS5

MacromoleculeName: uS5 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 27.529238 KDa
SequenceString: MADDAGAAGG PGGPGGPGNK EWLPVTKLGR LVKDMKIKSL EEIYLFSLPI KESEIIDFFL GAALKDEVLK IMPVQKQTRA GQRTRFKAF VAIGDYNGHV GLGLKCSKEV ATAIRGAIIL AKLSIVPVRR GYWGNKIGKP HTVPCKVTGR CGSVLVRLIP A PRGTGIVS ...String:
MADDAGAAGG PGGPGGPGNK EWLPVTKLGR LVKDMKIKSL EEIYLFSLPI KESEIIDFFL GAALKDEVLK IMPVQKQTRA GQRTRFKAF VAIGDYNGHV GLGLKCSKEV ATAIRGAIIL AKLSIVPVRR GYWGNKIGKP HTVPCKVTGR CGSVLVRLIP A PRGTGIVS APVPKKLLLM AGIDDCYTSA RGCTATLGNF AKATFDAISK TYSYLTPDLW KETVFTKSPY QEFTNHLMKT HT RVSVQRT QAPAVATT

UniProtKB: Small ribosomal subunit protein uS5

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Macromolecule #5: eS4

MacromoleculeName: eS4 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 29.59683 KDa
SequenceString: MARGPKKHLK RVAAPKHWML DKLTGVFAPR PSTGPHKLRE CLPLIIFLRN KLKYALTGDE VKKICMQRFI KIDGKVRADI TYPAGFMDV ISIDKTGENF RLIYDTKGRF AVHRITPEEA KYKLCKVRKI FVGTKGIPHL VTHDARTIRY PDPLIKVNDT I QIDLETGK ...String:
MARGPKKHLK RVAAPKHWML DKLTGVFAPR PSTGPHKLRE CLPLIIFLRN KLKYALTGDE VKKICMQRFI KIDGKVRADI TYPAGFMDV ISIDKTGENF RLIYDTKGRF AVHRITPEEA KYKLCKVRKI FVGTKGIPHL VTHDARTIRY PDPLIKVNDT I QIDLETGK ITDFIKFDTG NLCMVTGGAN LGRIGVITNR ERHPGSFDVV HVKDANGNSF ATRLSNIFVI GKGNKPWISL PR GKGIRLT IAEERDKRLA AKQSSG

UniProtKB: Small ribosomal subunit protein eS4

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Macromolecule #6: eS6

MacromoleculeName: eS6 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 28.751906 KDa
SequenceString: MKLNISFPAT GCQKLIEVDD ERKLRTFYEK RMATEVAADA LGEEWKGYVV RISGGNDKQG FPMKQGVLTH GRVRLLLSKG HSCYRPRRT GERKRKSVRG CIVDANLSVL NLVIVKKGEK DIPGLTDTTV PRRLGPKRAS RIRKLFNLSK EDDVRQYVVR K PLNKEGKK ...String:
MKLNISFPAT GCQKLIEVDD ERKLRTFYEK RMATEVAADA LGEEWKGYVV RISGGNDKQG FPMKQGVLTH GRVRLLLSKG HSCYRPRRT GERKRKSVRG CIVDANLSVL NLVIVKKGEK DIPGLTDTTV PRRLGPKRAS RIRKLFNLSK EDDVRQYVVR K PLNKEGKK PRTKAPKIQR LVTPRVLQHK RRRIALKKQR TKKNKEEAAE YAKLLAKRMK EAKEKRQEQI AKRRRLSSLR AS TSKSESS QK

UniProtKB: Small ribosomal subunit protein eS6

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Macromolecule #7: eS7

MacromoleculeName: eS7 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 22.168914 KDa
SequenceString: MFSSSAKIVK PNGEKPDEFE SGISQALLEL EMNSDLKAQL RELNITAAKE IEVGGGRKAI IIFVPVPQLK SFQKIQVRLV RELEKKFSG KHVVFIAQRR ILPKPTRKSR TKNKQKRPRS RTLTAVHDAI LEDLVFPSEI VGKRIRVKLD GSRLIKVHLD K AQQNNVEH ...String:
MFSSSAKIVK PNGEKPDEFE SGISQALLEL EMNSDLKAQL RELNITAAKE IEVGGGRKAI IIFVPVPQLK SFQKIQVRLV RELEKKFSG KHVVFIAQRR ILPKPTRKSR TKNKQKRPRS RTLTAVHDAI LEDLVFPSEI VGKRIRVKLD GSRLIKVHLD K AQQNNVEH KVETFSGVYK KLTGKDVNFE FPEFQL

UniProtKB: Small ribosomal subunit protein eS7

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Macromolecule #8: eS8

MacromoleculeName: eS8 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 24.263387 KDa
SequenceString: MGISRDNWHK RRKTGGKRKP YHKKRKYELG RPAANTKIGP RRIHTVRVRG GNKKYRALRL DVGNFSWGSE CCTRKTRIID VVYNASNNE LVRTKTLVKN CIVLIDSTPY RQWYESHYAL PLGRKKGAKL TPEEEEILNK KRSKKIQKKY DERKKNAKIS S LLEEQFQQ ...String:
MGISRDNWHK RRKTGGKRKP YHKKRKYELG RPAANTKIGP RRIHTVRVRG GNKKYRALRL DVGNFSWGSE CCTRKTRIID VVYNASNNE LVRTKTLVKN CIVLIDSTPY RQWYESHYAL PLGRKKGAKL TPEEEEILNK KRSKKIQKKY DERKKNAKIS S LLEEQFQQ GKLLACIASR PGQCGRADGY VLEGKELEFY LRKIKARKGK

UniProtKB: Small ribosomal subunit protein eS8

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Macromolecule #9: uS4

MacromoleculeName: uS4 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 22.641564 KDa
SequenceString: MPVARSWVCR KTYVTPRRPF EKSRLDQELK LIGEYGLRNK REVWRVKFTL AKIRKAAREL LTLDEKDPRR LFEGNALLRR LVRIGVLDE GKMKLDYILG LKIEDFLERR LQTQVFKLGL AKSIHHARVL IRQRHIRVRK QVVNIPSFIV RLDSQKHIDF S LRSPYGGG ...String:
MPVARSWVCR KTYVTPRRPF EKSRLDQELK LIGEYGLRNK REVWRVKFTL AKIRKAAREL LTLDEKDPRR LFEGNALLRR LVRIGVLDE GKMKLDYILG LKIEDFLERR LQTQVFKLGL AKSIHHARVL IRQRHIRVRK QVVNIPSFIV RLDSQKHIDF S LRSPYGGG RPGRVKRKNA KKGQGGAGAG DDEEED

UniProtKB: Small ribosomal subunit protein uS4

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Macromolecule #10: uS17

MacromoleculeName: uS17 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 18.468826 KDa
SequenceString:
MADIQTERAY QKQPTIFQNK KRVLLGETGK EKLPRYYKNI GLGFKTPKEA IEGTYIDKKC PFTGNVSIRG RILSGVVTKM KMQRTIVIR RDYLHYIRKY NRFEKRHKNM SVHLSPCFRD VQIGDIVTVG ECRPLSKTVR FNVLKVTKAA GTKKQFQKF

UniProtKB: Small ribosomal subunit protein uS17

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Macromolecule #11: uS15

MacromoleculeName: uS15 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 17.259389 KDa
SequenceString:
MGRMHAPGKG LSQSALPYRR SVPTWLKLTS DDVKEQIYKL AKKGLTPSQI GVILRDSHGV AQVRFVTGNK ILRILKSKGL APDLPEDLY HLIKKAVAVR KHLERNRKDK DAKFRLILIE SRIHRLARYY KTKRVLPPNW KYESSTASAL VA

UniProtKB: Small ribosomal subunit protein uS15

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Macromolecule #12: uS11

MacromoleculeName: uS11 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 16.302772 KDa
SequenceString:
MAPRKGKEKK EEQVISLGPQ VAEGENVFGV CHIFASFNDT FVHVTDLSGK ETICRVTGGM KVKADRDESS PYAAMLAAQD VAQRCKELG ITALHIKLRA TGGNRTKTPG PGAQSALRAL ARSGMKIGRI EDVTPIPSDS TRRKGGRRGR RL

UniProtKB: Small ribosomal subunit protein uS11

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Macromolecule #13: eS21

MacromoleculeName: eS21 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 9.098287 KDa
SequenceString:
MQSDAGEFVD LYVPRKCSAS NRIIGAKDHA SIQMNVAEVD KVTGRFNGQF KTYAICGAIR RMGESDDSIL RLAKSDGIVS NNF

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Macromolecule #14: uS8

MacromoleculeName: uS8 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 14.865555 KDa
SequenceString:
MVRMNVLADA LKSINNAEKR GKRQVLIRPC SKVIVRFLTV MMKHGYIGEF EIIDDHRAGK IVVNLTGRLN KCGVISPRFD VQLKDLEKW QNNLLPSRQF GFIVLTTSAG IMDHEEARRK HTGGKILGFF F

UniProtKB: Small ribosomal subunit protein uS8

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Macromolecule #15: uS12

MacromoleculeName: uS12 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 15.784549 KDa
SequenceString:
AGKCRGLRTA RKLRSHRRDQ KWHDKQYKKA HLGTALKANP FGGASHAKGI VLEKVGVEAK QPNSAIRKCV RVQLIKNGKK ITAFVPNDG CLNFIEENDE VLVAGFGRKG HAVGDIPGVR FKVVKVANVS LLALYKGKKE RPRS

UniProtKB: Small ribosomal subunit protein uS12

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Macromolecule #16: eS24

MacromoleculeName: eS24 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 15.548398 KDa
SequenceString:
MNDTVTIRTR KFMTNRLLQR KQMVIDVLHP GKATVPKTEI REKLAKMYKT TPDVIFVFGF RTHFGGGKTT GFGMIYDSLD YAKKNEPKH RLARHGLYEK KKTSRKQRKE RKNRMKKVRG TAKANVGAGK KEPRG

UniProtKB: 40S ribosomal protein S24

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Macromolecule #17: eS26

MacromoleculeName: eS26 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 13.015506 KDa
SequenceString:
MTKKRRNNGR AKKGRGHVQP IRCTNCARCV PKDKAIKKFV IRNIVEAAAV RDISEVSVFD AYVLPKLYVK LHYCVSCAIH SKVVRNRSR EARKDRTPPP RFRPAGAAPP PPPKPM

UniProtKB: Small ribosomal subunit protein eS26

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Macromolecule #18: eS27

MacromoleculeName: eS27 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 9.480186 KDa
SequenceString:
MPLAKDLLHP SPEEEKRKHK KKRLVQSPNS YFMDVKCPGC YKITTVFSHA QTVVLCVGCS TVLCQPTGGK ARLTEGCSFR RKQH

UniProtKB: Small ribosomal subunit protein eS27

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Macromolecule #19: eS30

MacromoleculeName: eS30 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 14.498884 KDa
SequenceString:
MQLFVRAQEL HTLEVTGRET VAQIKAHVAS LEGIAPEDQV VLLAGTPLED EATLGQCGVE ALSTLEVAGR MLGGKVHGSL ARVGKVRGQ TLKVAKQEKK KKRTGRAKRR MQYNRRFVNV VPTFGKKKGP NANS

UniProtKB: Ubiquitin-like FUBI-ribosomal protein eS30 fusion protein

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Macromolecule #20: uS3

MacromoleculeName: uS3 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 31.146607 KDa
SequenceString: MSARRRRRRA AFRRRAEPFI PISVREPLPF LSAARGGKMA VQISKKRKFV ADGIFKAELN EFLTRELAED GYSGVEVRVT PTRTEIIIL ATRTQNVLGE KGRRIRELTA VVQKRFGFPE GSVELYAEKV ATRGLCAIAQ AESLRYKLLG GLAVRRACYG V LRFIMESG ...String:
MSARRRRRRA AFRRRAEPFI PISVREPLPF LSAARGGKMA VQISKKRKFV ADGIFKAELN EFLTRELAED GYSGVEVRVT PTRTEIIIL ATRTQNVLGE KGRRIRELTA VVQKRFGFPE GSVELYAEKV ATRGLCAIAQ AESLRYKLLG GLAVRRACYG V LRFIMESG AKGCEVVVSG KLRGQRAKSM KFVDGLMIHS GDPVNYYVDT AVRHVLLRQG VLGIKVKIML PWDPSGKIGP KK PLPDHVS IVEPKDEILP TTPISEQKGG KPEPPAMPQP VPTA

UniProtKB: Small ribosomal subunit protein uS3

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Macromolecule #21: uS7

MacromoleculeName: uS7 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 22.913453 KDa
SequenceString: MTEWETAAPA VAETPDIKLF GKWSTDDVQI NDISLQDYIA VKEKYAKYLP HSAGRYAAKR FRKAQCPIVE RLTNSMMMHG RNNGKKLMT VRIVKHAFEI IHLLTGENPL QVLVNAIINS GPREDSTRIG RAGTVRRQAV DVSPLRRVNQ AIWLLCTGAR E AAFRNIKT ...String:
MTEWETAAPA VAETPDIKLF GKWSTDDVQI NDISLQDYIA VKEKYAKYLP HSAGRYAAKR FRKAQCPIVE RLTNSMMMHG RNNGKKLMT VRIVKHAFEI IHLLTGENPL QVLVNAIINS GPREDSTRIG RAGTVRRQAV DVSPLRRVNQ AIWLLCTGAR E AAFRNIKT IAECLADELI NAAKGSSNSY AIKKKDELER VAKSNR

UniProtKB: Small ribosomal subunit protein uS7

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Macromolecule #22: eS10

MacromoleculeName: eS10 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 17.156865 KDa
SequenceString:
MLMPKKNRIA IYELLFKEGV MVAKKDVHMP KHPELADKNV PNLHVMKAMQ SLKSRGYVKE QFAWRHFYWY LTNEGIQYLR DYLHLPPEI VPATLRRSRP ETGRPRPKGL EGERPARLTR GEADRDTYRR SAVPPGADKK AEAGAGSATE

UniProtKB: Plectin/eS10 N-terminal domain-containing protein

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Macromolecule #23: eS12

MacromoleculeName: eS12 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 14.538987 KDa
SequenceString:
MAEEGIAAGG VMDVNTALQE VLKTALIHDG LARGIREAAK ALDKRQAHLC VLASNCDEPM YVKLVEALCA EHQINLIKVD DNKKLGEWV GLCKIDREGK PRKVVGCSCV VVKDYGKESQ AKDVIEEYFK CKK

UniProtKB: Small ribosomal subunit protein eS12

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Macromolecule #24: uS19

MacromoleculeName: uS19 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 17.049182 KDa
SequenceString:
MAEVEQKKKR TFRKFTYRGV DLDQLLDMSY EQLMQLYSAR QRRRLSRGLR RKQHSLLKRL RKAKKEAPPM EKPEVVKTHL RDMIILPEM VGSMVGVYNG KTFNQVEIKP EMIGHYLGEF SITYKPVKHG RPGIGATHSS RFIPLK

UniProtKB: Small ribosomal subunit protein uS19

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Macromolecule #25: uS9

MacromoleculeName: uS9 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 19.213465 KDa
SequenceString:
SARVAPRFPF PVVAPRGEDR HSSAAAMPSK GPLQSVQVFG RKKTATAVAH CKRGNGLIKV NGRPLEMIEP RTLQYKLLEP VLLLGKERF AGVDIRVRVK GGGHVAQIYA IRQSISKALV AYYQKYVDEA SKKEIKDILI QYDRTLLVAD PRRCESKKFG G PGARARYQ KSYR

UniProtKB: Small ribosomal subunit protein uS9

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Macromolecule #26: eS17

MacromoleculeName: eS17 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 15.552119 KDa
SequenceString:
MGRVRTKTVK KAARVIIEKY YTRLGNDFHT NKRVCEEIAI IPSKKLRNKI AGYVTHLMKR IQRGPVRGIS IKLQEEERER RDNYVPEVS ALDQEIIEVD PDTKEMLKLL DFGSLSNLQV TQPTVGMNFK TPRGAV

UniProtKB: Small ribosomal subunit protein eS17

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Macromolecule #27: uS13

MacromoleculeName: uS13 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 17.759777 KDa
SequenceString:
MSLVIPEKFQ HILRVLNTNI DGRRKIAFAI TAIKGVGRRY AHVVLRKADI DLTKRAGELT EDEVERVITI MQNPRQYKIP DWFLNRQKD VKDGKYSQVL ANGLDNKLRE DLERLKKIRA HRGLRHFWGL RVRGQHTKTT GRRGRTVGVS KKK

UniProtKB: Small ribosomal subunit protein uS13

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Macromolecule #28: eS19

MacromoleculeName: eS19 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 16.235796 KDa
SequenceString:
MPGVTVKDVN QQEFVRALAA FLKKSGKLKV PEWVDTVKLA KHKELAPYDE NWFYTRAAST ARHLYLRGGA GVGSMTKIYG GRQRNGVMP SHFSRGSKSV ARRVLQALEG LKMVEKDQDW GRKLTPQGQR DLDRIAGQVA AAKKKH

UniProtKB: Small ribosomal subunit protein eS19

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Macromolecule #29: uS10

MacromoleculeName: uS10 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 13.398763 KDa
SequenceString:
MAFKDTGKTP VEPEVAIHRI RITLTSRNVK SLEKVCADLI RGAKEKNLKV KGPVRMPTKT LRITTRKTPC GEGSKTWDRF QMRIHKRLI DLHSPSEIVK QITSISIEPG VEVEVTIADA

UniProtKB: Small ribosomal subunit protein uS10

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Macromolecule #30: eS25

MacromoleculeName: eS25 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 13.776224 KDa
SequenceString:
MPPKDDKKKK DAGKSAKKDK DPVNKSGGKA KKKKWSKGKV RDKLNNLVLF DKATYDKLCK EVPNYKLITP AVVSERLKIR GSLARAALQ ELLSKGLIKL VSKHRAQVIY TRNTKGGDAP AAGEDA

UniProtKB: Small ribosomal subunit protein eS25

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Macromolecule #31: eS28

MacromoleculeName: eS28 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 7.855052 KDa
SequenceString:
MDTSRVQPIK LARVTKVLGR TGSQGQCTQV RVEFMDDTSR SIIRNVKGPV REGDVLTLLE SEREARRLR

UniProtKB: Small ribosomal subunit protein eS28

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Macromolecule #32: eS29

MacromoleculeName: eS29 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 6.690821 KDa
SequenceString:
MGHQQLYWSH PRKFGQGSRS CRVCSNRHGL IRKYGLNMCR QCFRQYAKDI GFIKLD

UniProtKB: Small ribosomal subunit protein uS14

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Macromolecule #33: eS31

MacromoleculeName: eS31 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 18.004041 KDa
SequenceString:
MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGGAKKR KKKSYTTPK KNKHKRKKVK LAVLKYYKVD ENGKISRLRR ECPSDECGAG VFMASHFDRH YCGKCCLTYC FNKPEDK

UniProtKB: Ubiquitin-ribosomal protein eS31 fusion protein

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Macromolecule #34: RACK1

MacromoleculeName: RACK1 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 35.115652 KDa
SequenceString: MTEQMTLRGT LKGHNGWVTQ IATTPQFPDM ILSASRDKTI IMWKLTRDET NYGIPQRALR GHSHFVSDVV ISSDGQFALS GSWDGTLRL WDLTTGTTTR RFVGHTKDVL SVAFSSDNRQ IVSGSRDKTI KLWNTLGVCK YTVQDESHSE WVSCVRFSPN S SNPIIVSC ...String:
MTEQMTLRGT LKGHNGWVTQ IATTPQFPDM ILSASRDKTI IMWKLTRDET NYGIPQRALR GHSHFVSDVV ISSDGQFALS GSWDGTLRL WDLTTGTTTR RFVGHTKDVL SVAFSSDNRQ IVSGSRDKTI KLWNTLGVCK YTVQDESHSE WVSCVRFSPN S SNPIIVSC GWDKLVKVWN LANCKLKTNH IGHTGYLNTV TVSPDGSLCA SGGKDGQAML WDLNEGKHLY TLDGGDIINA LC FSPNRYW LCAATGPSIK IWDLEGKIIV DELKQEVIST SSKAEPPQCT SLAWSADGQT LFAGYTDNLV RVWQVTIGTR

UniProtKB: Small ribosomal subunit protein RACK1

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Macromolecule #35: Eukaryotic translation initiation factor 3 subunit E

MacromoleculeName: Eukaryotic translation initiation factor 3 subunit E / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 54.199863 KDa
SequenceString: MLKIFTCNSH SDLPTNLMAE YDLTTRIAHF LDRHLVFPLL EFLSVKEIYN EKELLQGKLD LLSDTNMVDF AMDVYKNLYS DDIPHALRE KRTTVVAQLK QLQAETEPIV KMFEDPETTR QMQSTRDGRM LFDYLADKHG FRQEYLDTLY RYAKFQYECG N YSGAAEYL ...String:
MLKIFTCNSH SDLPTNLMAE YDLTTRIAHF LDRHLVFPLL EFLSVKEIYN EKELLQGKLD LLSDTNMVDF AMDVYKNLYS DDIPHALRE KRTTVVAQLK QLQAETEPIV KMFEDPETTR QMQSTRDGRM LFDYLADKHG FRQEYLDTLY RYAKFQYECG N YSGAAEYL YFFRVLVPAT DRNALSSLWG KLASEILMQN WDAAMEDLTR LKETIDNNSV SSPLQSLQQR TWLIHWSLFV FF NHPKGRD NIIDLFLYQP QYLNAIQTMC PHILRYLTTA VITNKDVRKR RQVLKDLVKV IQQESYTYKD PITEFVECLY VNF DFDGAQ KKLRECESVL VNDFFLVACL EDFIENARLF IFETFCRIHQ CISINMLADK LNMTPEEAER WIVNLIRNAR LDAK IDSKL GHVVMGNNAV SPYQQVIEKT KSLSFRSQML AMNIEKKLNQ NSRSEAPNWA TQDSGFY

UniProtKB: Eukaryotic translation initiation factor 3 subunit E

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Macromolecule #36: Eukaryotic translation initiation factor 3 subunit F

MacromoleculeName: Eukaryotic translation initiation factor 3 subunit F / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 37.84673 KDa
SequenceString: MATPAVSASA PPATPAATPA AAAAPASAPA AAPAPTPAPA PAAAPAAGAA PAAAADPAAA AAAAGTGQTP ASAQAPAQTS APSLPGPAL PGPFPGGRVV RLHPVILASI VDSYERRNEG AARVIGTLLG TVDKHSVEVT NCFSVPHNES EDEVAVDMEF A KNMYELHK ...String:
MATPAVSASA PPATPAATPA AAAAPASAPA AAPAPTPAPA PAAAPAAGAA PAAAADPAAA AAAAGTGQTP ASAQAPAQTS APSLPGPAL PGPFPGGRVV RLHPVILASI VDSYERRNEG AARVIGTLLG TVDKHSVEVT NCFSVPHNES EDEVAVDMEF A KNMYELHK KVSPNELILG WYATGHDITE HSVLIHEYYS REAPNPIHLT VDTSLQNGRM SIKAYVSTSM GVPGRTMGVM FT PLTVKYA YYDTERIGVD LIMKTCFSPN RVIGLSSDLQ QVGGASARIQ DALSTVLQYA EDVLSGKVSA DNTVGRFLMS LVN QVPKIV PDDFETMLNS NINDLLMVTY LANLTQSQIA LNEKLVNL

UniProtKB: Eukaryotic translation initiation factor 3 subunit F

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Macromolecule #37: Eukaryotic translation initiation factor 3 subunit K

MacromoleculeName: Eukaryotic translation initiation factor 3 subunit K / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 25.129709 KDa
SequenceString: MAMFEQMRAN VGKLLKGIDR YNPENLATLE RYVETQAKEN AYDLEANLAV LKLYQFNPAF FQTTVTAQIL LKALTNLPHT DFTLCKCMI DQAHQEERPI RQILYLGDLL ETCHFQAFWQ ALDENMDLLE GITGFEDSVR KFICHVVGIT YQHIDRWLLA E MLGDLTDS ...String:
MAMFEQMRAN VGKLLKGIDR YNPENLATLE RYVETQAKEN AYDLEANLAV LKLYQFNPAF FQTTVTAQIL LKALTNLPHT DFTLCKCMI DQAHQEERPI RQILYLGDLL ETCHFQAFWQ ALDENMDLLE GITGFEDSVR KFICHVVGIT YQHIDRWLLA E MLGDLTDS QLKMWMSKYG WSADESGQIF ICSQEESIKP KNIVEKIDFD SVSSIMASSQ

UniProtKB: Eukaryotic translation initiation factor 3 subunit K

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Macromolecule #38: Eukaryotic translation initiation factor 3 subunit L

MacromoleculeName: Eukaryotic translation initiation factor 3 subunit L / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 71.001477 KDa
SequenceString: PAGGCAQTSA FPAGPRRAEL PAPRRTRGSL SSLSPAVFAS AAAMSYPADD YESEAVYDPY AYPSDYDMHT GDPKQDLAYE RQYEQQTYQ VIPEVIRNFI QYFHKTVSDL IDQKVYELQA SRVSSDVIDQ KVYEIQDIYE NSWTKLTERF FKNTPWPEAE T IAPQVGND ...String:
PAGGCAQTSA FPAGPRRAEL PAPRRTRGSL SSLSPAVFAS AAAMSYPADD YESEAVYDPY AYPSDYDMHT GDPKQDLAYE RQYEQQTYQ VIPEVIRNFI QYFHKTVSDL IDQKVYELQA SRVSSDVIDQ KVYEIQDIYE NSWTKLTERF FKNTPWPEAE T IAPQVGND AVFLILYKEL YYRHIYAKVS GGPSLAQRFE SYYNYCNLFN YILNADGPAP LELPNQWLWD IIDEFIYQFQ SF SQYRCKT AKKSEEEIDF LRSNPKIWNV HSVLNVLHSL VDKSNINRQL EVYTSGGDPE SVAGEYGRHS LYKMLGYFSL VGL LRLHSL LGDYYQAIKV LENIELNKKS MYSRVPECQV TTYYYVGFAY LMMRRYQDAI RVFANILLYI QRTKSMFQRT TYKY EMINK QNEQMHALLA IALTMYPMRI DESIHLQLRE KYGDKMLRMQ KGDPQVYEEL FSYSCPKFLS PVVPNYDSVH PNYHK EPFL QQLKVFSDEV QQQAQLSTIR SFLKLYTTMP VAKLAGFLDL TEQEFRIQLL VFKHKMKNLV WTSGISALDG EFQSAS EVD FYIDKDMIHI ADTKVARRYG DFFIRQIHKF EELNRTLKKM GQRP

UniProtKB: Eukaryotic translation initiation factor 3 subunit L

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Macromolecule #39: Eukaryotic translation initiation factor 3 subunit M

MacromoleculeName: Eukaryotic translation initiation factor 3 subunit M / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 42.555832 KDa
SequenceString: MSVPAFIDIS EEDQAAELRA YLKSKGAEIS EENSEGGLHV DLAQIIEACD VCLKEDDKDV ESVMNSVVSL LLILEPDKQE ALIESLCEK LVKFREGERP SLRLQLLSNL FHGMDKNTPV RYTVYCSLIK VAASCGAIQY IPTELDQVRK WISDWNLTTE K KHTLLRLL ...String:
MSVPAFIDIS EEDQAAELRA YLKSKGAEIS EENSEGGLHV DLAQIIEACD VCLKEDDKDV ESVMNSVVSL LLILEPDKQE ALIESLCEK LVKFREGERP SLRLQLLSNL FHGMDKNTPV RYTVYCSLIK VAASCGAIQY IPTELDQVRK WISDWNLTTE K KHTLLRLL YEALVDCKKS DAASKVMVEL LGSYTEDNAS QARVDAHRCI VRALKDPNAF LFDHLLTLKP VKFLEGELIH DL LTIFVSA KLASYVKFYQ NNKDFIDSLG LLHEQNMAKM RLLTFMGMAV ENKEISFDTM QQELQIGADD VEAFVIDAVR TKM VYCKID QTQRKVVVSH STHRTFGKQQ WQQLYDTLNA WKQNLNKVKN SLLSLSDT

UniProtKB: Eukaryotic translation initiation factor 3 subunit M

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Macromolecule #40: Eukaryotic translation initiation factor 3 subunit A

MacromoleculeName: Eukaryotic translation initiation factor 3 subunit A / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 164.902656 KDa
SequenceString: MPAYFQRPEN ALKRANEFLE VGKKQPALDV LYDVMKSKKH RTWQKIHEPI MLKYLELCVD LRKSHLAKEG LYQYKNICQQ VNIKSLEDV VRAYLKLAEE KTEAAKEESQ QMVLDIEDLD NIQTPESVLL SAVSGEDTQD RTDRLLLTPW VKFLWESYRQ C LDLLRNNS ...String:
MPAYFQRPEN ALKRANEFLE VGKKQPALDV LYDVMKSKKH RTWQKIHEPI MLKYLELCVD LRKSHLAKEG LYQYKNICQQ VNIKSLEDV VRAYLKLAEE KTEAAKEESQ QMVLDIEDLD NIQTPESVLL SAVSGEDTQD RTDRLLLTPW VKFLWESYRQ C LDLLRNNS RVERLYHDIA QQAFKFCLQY TRKAEFRKLC DNLRMHLSQI QRHHNQSTAI NLNNPESQSM HLETRLVQLD SA ISMELWQ EAFKAVEDIH GLFSLSKKPP KPQLMANYYN KVSTVFWKSG NALFHASTLH RLYHLSREMR KNLTQDEMQR MST RVLLAT LSIPITPERT DIARLLDMDG IIVEKQRRLA TLLGLQAPPT RIGLINDMVR FNVLQYVVPE VKDLYNWLEV EFNP LKLCE RVTKVLNWVR EQPEKEPELQ QYVPQLQSNT ILRLLQQVAQ IYQSIEFSRL TSLVPFVDAF QLERAIVDAA RHCDL QVRI DHTSRTLSFG SDLNYATRED APIGPHLQSM PSEQIRNQLT AMSSVLAKAL EVIKPAHILQ EKEEQHQLAV TAYLKN SRK EHQRILARRQ TIEERKERLE SLNIQREKEE LEQREAELQK VRKAEEERLR QEAKEREKER ILQEHEQIKK KTVRERL EQ IKKTELGAKA FKDIDIEDLE ELDPDFIMAK QVEQLEKEKK ELQERLKNQE KKIDYFERAK RLEEIPLIKS AYEEQRIK D MDLWEQQEEE RITTMQLERE KALEHKNRMS RMLEDRDLFV MRLKAARQSV YEEKLKQFEE RLAEERHNRL EERKRQRKE ERRITYYREK EEEEQRRAEE QMLKEREERE RAERAKREEE LREYQERVKK LEEVERKKRQ RELEIEERER RREEERRLGE DPLSRRDSR WGDRDSEGTW RKGPEADSEW RRGPPEKEWR RGEGRDDERS HRRDEERPRR VGDDEDRESS LRPDDDRTLR R GMEDDRGP RRGPDEERFS RRGLEDERPS WRSTDDDRPP RRIGEEDRGS WRHAEDDRPP RRGLDDDRGS WRTADEDRGP RR MMDEDRG PRRGGADDER PSWRSADDDR GPRRGIDDDR GPRRGLDDDR GPWRNADDDR IPRRGADDDR GPWRNMDDDR MSR RADDDR ISRRGEDSRP GPWRPFVKPG GWREKEKARE ESWGPPRESR PPEEREWERE RDRDNPDREE NDKDPERERD RERD GDREE RFRRPRDEGG WRRGPAEEAS SWRDSSRRDD RDRDDRRRER DDRRDLRDRR DLRDDRDRRG PPLRSERDEV SSWRR ADDR KDDRAEERDP PRRVPPPVLS RDRDRDRDRD RDRDRERERE REREGEKEKT SWRAEKDRES LRRTKNETDE DGWTTV RR

UniProtKB: Eukaryotic translation initiation factor 3 subunit A

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Macromolecule #41: Eukaryotic translation initiation factor 3 subunit C

MacromoleculeName: Eukaryotic translation initiation factor 3 subunit C / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 105.706156 KDa
SequenceString: MSRFFTTGSD SESESSLSGE ELVTKPVGGN YGKQPLLLSE DEEDTKRVVR SAKDKRFEEL TNLIRTIRNA MKIRDVTKCL EEFELLGKA YGKAKSIVDK EGVPRFYIRI LADLEDYLNE LWEDKEGKKK MNKNNAKALS TLRQKIRKYN RDFESHITNY K QNPEQSAD ...String:
MSRFFTTGSD SESESSLSGE ELVTKPVGGN YGKQPLLLSE DEEDTKRVVR SAKDKRFEEL TNLIRTIRNA MKIRDVTKCL EEFELLGKA YGKAKSIVDK EGVPRFYIRI LADLEDYLNE LWEDKEGKKK MNKNNAKALS TLRQKIRKYN RDFESHITNY K QNPEQSAD EDAEKNEEDS EGSSDEDEDE DGVSAAAFLK KKAEAPSGES RKFLKKMEDE DEDSEDSEDD EEWDTGSTSS DS DSEEEEG KQTALASRFL KKAPTTEEDK KAAEKKREDK AKKKHDRKSK RLDEEEEDNE GGEWERVRGG VPLVKEKPKM FAK GTEITH AVVIKKLNEI LQARGKKGTD RAAQIELLQL LVQIASENNL GEGVIVKIKF NIIASLYDYN PNLATYMKPE MWQK CLDCI NELMDILFAN PNIFVGENIL EESENLQNVD QPLRVRGCIL TLVERMDEEF TKIMQNTDPH SQEYVEHLKD EAQVC AIIE RVQRYLEEKG TTEEICRVYL RRILHTYYKF DYKAHQRQLT PPEGSSKSEQ DQAENEGEDS AVLMERLCKY IYAKDR TDR IRTCAILCHI YHHALHSRWY QARDLMLMSH LQDNIQHADP PVQILYNRTM VQLGICAFRQ GLTKDAHNAL LDIQSSG RA KELLGQGLLL RSLQERNQEQ EKVERRRQEP FHLHINLELL ECVYLVSAML LEIPYMAAHE SDARRRMISK QFHHQLRV G ERQPLLGPPE SMREHVVAAS KAMKMGDWKT CHSFIINEKM NGKVWDLFPE ADKVRTMLVR KIQEESLRTY LFTYSSVYD SISMETLSDM FELDLPTVHS IISKMIINEE LMASLDQPTQ TVVMHRTEPT AQQNLALQLA EKLGSLVENN ERVFDHKQGT YGGYFRDQK DGYRKNEGYM RRGGYRQQQS QTAY

UniProtKB: Eukaryotic translation initiation factor 3 subunit C

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Macromolecule #42: Eukaryotic translation initiation factor 3 subunit D

MacromoleculeName: Eukaryotic translation initiation factor 3 subunit D / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 64.517199 KDa
SequenceString: MANDENTDGV NKSEKMEKMC EFIRPMVQNN PDGWGPCELP DQFKDIPYQP FSKSDRLGKI SDWTGTAYQD KKFQNKYNSQ FGGSGMKAY AYEHDEDETT FHLVDTTRVQ KPPYQRGRFG QRNQRMRGRG GQRGGMSQMQ ALGKLKIHER DRRGQNKRWT R RNQAPIKM ...String:
MANDENTDGV NKSEKMEKMC EFIRPMVQNN PDGWGPCELP DQFKDIPYQP FSKSDRLGKI SDWTGTAYQD KKFQNKYNSQ FGGSGMKAY AYEHDEDETT FHLVDTTRVQ KPPYQRGRFG QRNQRMRGRG GQRGGMSQMQ ALGKLKIHER DRRGQNKRWT R RNQAPIKM RDASVTVRPD WVTIEEMDFP RLSKLTLPGV KEGEDVLCCG AVEYYDKSYD RVNVKNEKPL QRIDRIFHTV TT TDDPVIR KLSKTEGNVY ATDAILATIM CCTRSNYSWD IVIEKIGNKL FFDKRDNTEF DLLTVNETSV EPPQDDGNSL NSP RNLALE ATFINHNFSQ QVLKSNEPRY KFDEPNPFIS EEEEGEVASV AYRYRKWDLN NGITLIARCE HDAVMQGPNN ETQF LTIKA LNEWDSKLAN GVEWRRKLDT QRGAVLANEL RNNACKLAKW TVQALLAGSD QLKFGYVSRA SVRDSSKHVI LETQQ YKPN EFATQINLNM DNAWGILRCI IDICMNQKDG KYLIMKDPNK PMIRLYDIPD NTFESEGEEE DSDEEEQVKD AFQR

UniProtKB: Eukaryotic translation initiation factor 3 subunit D

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Macromolecule #43: Eukaryotic translation initiation factor 3 subunit H

MacromoleculeName: Eukaryotic translation initiation factor 3 subunit H / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 41.083531 KDa
SequenceString: LPTAHAQSVP QMASRKEGTG SGGTSSSSAT GVVGKGKGKG GSGDSAVKQV QIDGLVVLKI IKHYQEEGQG TEVVQGVLLG LVVEDRLEI TNCFPFPQHT EDDADFDEVQ YQMEMMRSLR HVNIDHLHVG WYQSTYYGSF VTRALLDSQF SYQHAIEESV V LIYDPIKT ...String:
LPTAHAQSVP QMASRKEGTG SGGTSSSSAT GVVGKGKGKG GSGDSAVKQV QIDGLVVLKI IKHYQEEGQG TEVVQGVLLG LVVEDRLEI TNCFPFPQHT EDDADFDEVQ YQMEMMRSLR HVNIDHLHVG WYQSTYYGSF VTRALLDSQF SYQHAIEESV V LIYDPIKT AQGSLSLKAY RLTPKLMEVC KEKDFSPEAL KKANITFEHM FEEVPIVIKN SHLINVLMWE LEKKSAVADK HE LLSLASS NHLGKNLQLL MDRVDEMSQD IVKYNTYMRN TSKQQQQKHQ YQQRRQQENM QRQSRGEPPL PEEDLSKLFK PPQ PPARMD SLLIAGQINT YCQNIKEFTA QNLGKLFMAQ ALQEYNN

UniProtKB: Eukaryotic translation initiation factor 3 subunit H

+
Macromolecule #45: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 45 / Number of copies: 1 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #46: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 46 / Number of copies: 1 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
GridModel: Quantifoil, UltrAuFoil, R1.2/1.3 / Material: GOLD / Mesh: 300
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK IV
Details: Grids were blotted for 2.5s and flash cooled in liquid ethane.

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Digitization - Frames/image: 1-40 / Number grids imaged: 1 / Average exposure time: 8.0 sec. / Average electron dose: 56.9 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 915647
Startup modelType of model: EMDB MAP
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION
Final reconstructionNumber classes used: 1 / Applied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.36 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION / Number images used: 23444
DetailsThe microscope was equipped with an energy filter with slits aperture of 20eV, installed before the detector.
FSC plot (resolution estimation)

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