+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-0326 | ||||||||||||
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Title | Aeromonas hydrophila ExeD | ||||||||||||
Map data | ExeD 3D reconstruction | ||||||||||||
Sample |
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Function / homology | Function and homology information protein secretion by the type II secretion system / type II protein secretion system complex / cell outer membrane / membrane => GO:0016020 Similarity search - Function | ||||||||||||
Biological species | Aeromonas hydrophila (bacteria) | ||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.7 Å | ||||||||||||
Authors | Contreras-Martel C / Farias Estrozi L | ||||||||||||
Funding support | France, Canada, 3 items
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Citation | Journal: PLoS Pathog / Year: 2019 Title: Structure and assembly of pilotin-dependent and -independent secretins of the type II secretion system. Authors: S Peter Howard / Leandro F Estrozi / Quentin Bertrand / Carlos Contreras-Martel / Timothy Strozen / Viviana Job / Alexandre Martins / Daphna Fenel / Guy Schoehn / Andréa Dessen / Abstract: The type II secretion system (T2SS) is a cell envelope-spanning macromolecular complex that is prevalent in Gram-negative bacterial species. It serves as the predominant virulence mechanism of many ...The type II secretion system (T2SS) is a cell envelope-spanning macromolecular complex that is prevalent in Gram-negative bacterial species. It serves as the predominant virulence mechanism of many bacteria including those of the emerging human pathogens Vibrio vulnificus and Aeromonas hydrophila. The system is composed of a core set of highly conserved proteins that assemble an inner membrane platform, a periplasmic pseudopilus and an outer membrane complex termed the secretin. Localization and assembly of secretins in the outer membrane requires recognition of secretin monomers by two different partner systems: an inner membrane accessory complex or a highly sequence-diverse outer membrane lipoprotein, termed the pilotin. In this study, we addressed the question of differential secretin assembly mechanisms by using cryo-electron microscopy to determine the structures of the secretins from A. hydrophila (pilotin-independent ExeD) and V. vulnificus (pilotin-dependent EpsD). These structures, at approximately 3.5 Å resolution, reveal pentadecameric stoichiometries and C-terminal regions that carry a signature motif in the case of a pilotin-dependent assembly mechanism. We solved the crystal structure of the V. vulnificus EpsS pilotin and confirmed the importance of the signature motif for pilotin-dependent secretin assembly by performing modelling with the C-terminus of EpsD. We also show that secretin assembly is essential for membrane integrity and toxin secretion in V. vulnificus and establish that EpsD requires the coordinated activity of both the accessory complex EpsAB and the pilotin EpsS for full assembly and T2SS function. In contrast, mutation of the region of the S-domain that is normally the site of pilotin interactions has little effect on assembly or function of the ExeD secretin. Since secretins are essential outer membrane channels present in a variety of secretion systems, these results provide a structural and functional basis for understanding the key assembly steps for different members of this vast pore-forming family of proteins. | ||||||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_0326.map.gz | 7.1 MB | EMDB map data format | |
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Header (meta data) | emd-0326-v30.xml emd-0326.xml | 14.9 KB 14.9 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_0326_fsc.xml | 8.7 KB | Display | FSC data file |
Images | emd_0326.png | 241.7 KB | ||
Masks | emd_0326_msk_1.map | 59.6 MB | Mask map | |
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-0326 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-0326 | HTTPS FTP |
-Related structure data
Related structure data | 6i1xMC 0327C 6i1yC 6i2vC M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_0326.map.gz / Format: CCP4 / Size: 59.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | ExeD 3D reconstruction | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.21 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Mask #1
File | emd_0326_msk_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : ExeD
Entire | Name: ExeD |
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Components |
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-Supramolecule #1: ExeD
Supramolecule | Name: ExeD / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: Aeromonas hydrophila (bacteria) |
Recombinant expression | Organism: Cell-free gateway cloning vector N-term 8xHis pCellFree_G01 (others) Recombinant plasmid: pIVEX2.4T |
Molecular weight | Theoretical: 1 MDa |
-Macromolecule #1: Type II secretion system protein D
Macromolecule | Name: Type II secretion system protein D / type: protein_or_peptide / ID: 1 / Number of copies: 15 / Enantiomer: LEVO |
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Source (natural) | Organism: Aeromonas hydrophila (bacteria) |
Molecular weight | Theoretical: 55.618422 KDa |
Recombinant expression | Organism: Cell-free gateway cloning vector N-term 8xHis pCellFree_G01 (others) |
Sequence | String: GDEMVTRVVP VRNVSVRELA PLLRQLNDNA GGGNVVHYDP SNVLLITGRA AVVNRLVEVV RRVDKAGDQE VDIIKLKYAS AGEMVRLVT NLNKDGNSQG GNTSLLLAPK VVADERTNSV VVSGEPKARA RIIQMVRQLD RELQSQGNTR VFYLKYGKAK D MVEVLKGV ...String: GDEMVTRVVP VRNVSVRELA PLLRQLNDNA GGGNVVHYDP SNVLLITGRA AVVNRLVEVV RRVDKAGDQE VDIIKLKYAS AGEMVRLVT NLNKDGNSQG GNTSLLLAPK VVADERTNSV VVSGEPKARA RIIQMVRQLD RELQSQGNTR VFYLKYGKAK D MVEVLKGV SSSIEADKKG GGTATTAGGG ASIGGGKLAI SADETTNALV ITAQPDVMAE LEQVVAKLDI RRAQVLVEAI IV EIADGDG LNLGVQWANT NGGGTQFTNA GPGIGSVAIA AKDYKDNGTT TGLAKLAENF NGMAAGFYQG NWAMLVTALS TNT KSDILS TPSIVTMDNK EASFNVGQEV PVQTGTQNST SGDTTFSTIE RKTVGTKLVL TPQINEGDSV LLTIEQEVSS VGKQ ATGTD GLGPTFDTRT VKNAVLVKSG ETVVLGGLMD EQTKEEVSKV PLLGDIPVLG YLFRSTSNNT SKRNLMVFIR PTILR DANV YSGISSNKYT LFRAQQLDAV AQEGYATSPD RQVLPEYGQD |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Concentration | 10 mg/mL |
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Buffer | pH: 7.5 |
Grid | Model: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 400 / Support film - Material: CARBON / Support film - topology: HOLEY / Support film - Film thickness: 2.0 nm |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Instrument: FEI VITROBOT MARK IV |
Details | This sample was monodisperse |
-Electron microscopy
Microscope | FEI POLARA 300 |
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Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Calibrated magnification: 41322 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.0 mm |
Sample stage | Specimen holder model: GATAN LIQUID NITROGEN / Cooling holder cryogen: NITROGEN |
Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Number real images: 2247 / Average exposure time: 1.0 sec. / Average electron dose: 40.0 e/Å2 |
Experimental equipment | Model: Tecnai Polara / Image courtesy: FEI Company |