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- EMDB-0255: Molecular structure of promoter-bound yeast TFIID (locally-refine... -
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Open data
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Basic information
Entry | Database: EMDB / ID: 0255 |
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Title | Molecular structure of promoter-bound yeast TFIID (locally-refined Twin lobe) |
![]() | Twin lobe of KpTFIID |
![]() | Transcription Factor IID![]() |
Source | ![]() ![]() |
Method | ![]() ![]() |
![]() | Kolesnikova O / Ben-Shem A / Luo J / Ranish J / Schultz P / Papai G |
![]() | Journal: Nat Commun / Year: 2018 Title: Molecular structure of promoter-bound yeast TFIID. ![]() |
Date | Deposition: Sep 25, 2018 / Header (metadata) release: Oct 31, 2018 / Map release: Nov 21, 2018 / Last update: Nov 21, 2018 |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
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Download
![]() | ![]() |
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Header (meta data in XML format) | ![]() ![]() ![]() |
FSC (resolution estimation) | ![]() |
Images | ![]() |
Masks (Map data of sub-region, etc) | ![]() |
Others | ![]() ![]() |
FTP directory | ftp://ftp.pdbj.org/pub/emdb/structures/EMD-0255 |
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Links
-Related structure data
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.1 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Mask #1
File | ![]() | ||||||||||||
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Projections & Slices |
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Density Histograms |
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Sample components
-Entire Transcription Factor IID
Entire | Name: Transcription Factor IID / Number of components: 1 |
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-Component #1: protein, Transcription Factor IID
Protein | Name: Transcription Factor IID![]() |
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Mass | Theoretical: 900 kDa |
Source | Species: ![]() ![]() |
-Experimental details
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Sample preparation
Specimen | Specimen state: particle / Method: ![]() |
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Sample solution | Specimen conc.: 0.4 mg/ml / pH: 8 |
Vitrification | Instrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Temperature: 283 K / Humidity: 95 % |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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![]() | Microscope: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN![]() |
Lens | Magnification: 105000.0 X (nominal), 45454.0 X (calibrated) / Cs: 0.01 mm / Imaging mode: BRIGHT FIELD![]() |
Specimen Holder | Model: FEI TITAN KRIOS AUTOGRID HOLDER |
Camera | Detector: GATAN K2 SUMMIT (4k x 4k) |
-Image acquisition
Image acquisition | Sampling size: 5 microns |
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