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TitleMolecular structure of promoter-bound yeast TFIID.
Journal, issue, pagesNat Commun, Vol. 9, Issue 1, Page 4666, Year 2018
Publish dateNov 7, 2018
AuthorsOlga Kolesnikova / Adam Ben-Shem / Jie Luo / Jeff Ranish / Patrick Schultz / Gabor Papai /
PubMed AbstractTranscription preinitiation complex assembly on the promoters of protein encoding genes is nucleated in vivo by TFIID composed of the TATA-box Binding Protein (TBP) and 13 TBP-associate factors (Tafs) ...Transcription preinitiation complex assembly on the promoters of protein encoding genes is nucleated in vivo by TFIID composed of the TATA-box Binding Protein (TBP) and 13 TBP-associate factors (Tafs) providing regulatory and chromatin binding functions. Here we present the cryo-electron microscopy structure of promoter-bound yeast TFIID at a resolution better than 5 Å, except for a flexible domain. We position the crystal structures of several subunits and, in combination with cross-linking studies, describe the quaternary organization of TFIID. The compact tri lobed architecture is stabilized by a topologically closed Taf5-Taf6 tetramer. We confirm the unique subunit stoichiometry prevailing in TFIID and uncover a hexameric arrangement of Tafs containing a histone fold domain in the Twin lobe.
External linksNat Commun / PubMed:30405110 / PubMed Central
MethodsEM (single particle)
Resolution4.5 - 12.13 Å
Structure data

EMDB-0249:
Structure of K. phaffii TFIID
Method: EM (single particle) / Resolution: 12.13 Å

EMDB-0250:
Taf1 lobe of kpTFIID
Method: EM (single particle) / Resolution: 10.7 Å

0251

6hqa

EMDB-0251, PDB-6hqa:
Molecular structure of promoter-bound yeast TFIID
Method: EM (single particle) / Resolution: 7.1 Å

EMDB-0253:
Molecular structure of promoter-bound yeast TFIID (locally-refined Taf2 lobe)
Method: EM (single particle) / Resolution: 4.5 Å

EMDB-0254:
Molecular structure of promoter-bound yeast TFIID (locally-refined Taf2 lobe with two Taf6 HEAT repeats)
Method: EM (single particle) / Resolution: 4.6 Å

EMDB-0255:
Molecular structure of promoter-bound yeast TFIID (locally-refined Twin lobe)
Method: EM (single particle) / Resolution: 4.8 Å

Source
  • komagataella phaffii gs115 (fungus)
  • Yeast (fungus)
  • komagataella phaffii (strain gs115 / atcc 20864) (fungus)
KeywordsTRANSCRIPTION / Complex / Transcription initiation

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