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- PDB-5ykb: The N253F mutant structure of trehalose synthase from Deinococcus... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5ykb | |||||||||
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Title | The N253F mutant structure of trehalose synthase from Deinococcus radiodurans reveals an open active-site conformation | |||||||||
![]() | Trehalose synthase | |||||||||
![]() | ISOMERASE / trehalose synthase / glycoside hydrolase family 13 | |||||||||
Function / homology | ![]() maltose alpha-D-glucosyltransferase / maltose alpha-D-glucosyltransferase activity / carbohydrate metabolic process / metal ion binding Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Chow, S.Y. / Hsieh, Y.C. / Liaw, S.H. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: The N253F mutant structure of trehalose synthase from Deinococcus radiodurans reveals an open active-site topology Authors: Chow, S.Y. / Wang, Y.L. / Hsieh, Y.C. / Lee, G.C. / Liaw, S.H. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 427.5 KB | Display | ![]() |
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PDB format | ![]() | 347.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 475.8 KB | Display | ![]() |
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Full document | ![]() | 507.8 KB | Display | |
Data in XML | ![]() | 72.5 KB | Display | |
Data in CIF | ![]() | 99.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4tvuS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments: Component-ID: _ / Beg auth comp-ID: PRO / Beg label comp-ID: PRO / End auth comp-ID: ASN / End label comp-ID: ASN / Refine code: _ / Auth seq-ID: 6 - 552 / Label seq-ID: 8 - 554
NCS ensembles :
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Components
#1: Protein | Mass: 65020.914 Da / Num. of mol.: 4 / Mutation: R97W,N253F,T313I,I380V Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: R1 / Production host: ![]() ![]() References: UniProt: I3NX86, maltose alpha-D-glucosyltransferase #2: Chemical | ChemComp-CA / #3: Chemical | ChemComp-MG / #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.55 Å3/Da / Density % sol: 51.78 % |
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Crystal grow | Temperature: 288 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 7% PEG 4000, 0.2M sodium acetate trihydrate, 0.3M Tris-HCl (pH 7.0) |
-Data collection
Diffraction | Mean temperature: 110 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: RAYONIX MX300HE / Detector: CCD / Date: May 10, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.76→30 Å / Num. obs: 67645 / % possible obs: 99.9 % / Redundancy: 4.5 % / CC1/2: 0.993 / Net I/σ(I): 12.3 |
Reflection shell | Resolution: 2.76→2.86 Å / Redundancy: 4.4 % / Mean I/σ(I) obs: 1.8 / Num. unique obs: 6642 / CC1/2: 0.606 / % possible all: 99.3 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 4TVU Resolution: 2.76→30 Å / Cor.coef. Fo:Fc: 0.939 / Cor.coef. Fo:Fc free: 0.884 / SU B: 14.965 / SU ML: 0.298 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R Free: 0.374 Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 136.58 Å2 / Biso mean: 50.759 Å2 / Biso min: 2 Å2
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Refinement step | Cycle: final / Resolution: 2.76→30 Å
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Refine LS restraints |
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Refine LS restraints NCS | Refine-ID: X-RAY DIFFRACTION / Type: interatomic distance / Weight position: 0.05
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LS refinement shell | Resolution: 2.761→2.832 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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