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基本情報
登録情報 | ![]() |
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![]() | Glutamate/aspartate import solute-binding protein (DEBP) in the presence of glutamate - Glu-bound 10-fold excess
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機能・相同性 | ![]() aspartate binding / L-glutamate transmembrane transport / L-aspartate transmembrane transport / L-aspartate import across plasma membrane / L-glutamate import across plasma membrane / glutamate binding / ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing / outer membrane-bounded periplasmic space / membrane 類似検索 - 分子機能 |
生物種 | ![]() ![]() |
![]() | ![]() タイトル: Structure-based screening of binding affinities via small-angle X-ray scattering 著者: Chen P / Masiewicz P / Perez K |
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構造の表示
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-モデル
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試料
![]() | 名称: Glutamate/aspartate import solute-binding protein (DEBP) in the presence of glutamate - Glu-bound 10-fold excess 試料濃度: 2.2 mg/ml |
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バッファ | 名称: 100 mM NaCl, 20 mM NaPO4, 0.5 mM TCEP / pH: 7.4 / コメント: SEC buffer for PBP experiments |
要素 #1755 | タイプ: protein / 記述: Glutamate/aspartate import solute-binding protein / 分子量: 32.052 / 分子数: 1 / 由来: Escherichia coli (strain K12) / 参照: UniProt: P37902 配列: HHHHHHDDAA PAAGSTLDKI AKNGVIVVGH RESSVPFSYY DNQQKVVGYS QDYSNAIVEA VKKKLNKPDL QVKLIPITSQ NRIPLLQNGT FDFECGSTTN NVERQKQAAF SDTIFVVGTR LLTKKGGDIK DFANLKDKAV VVTSGTTSEV LLNKLNEEQK MNMRIISAKD ...配列: HHHHHHDDAA PAAGSTLDKI AKNGVIVVGH RESSVPFSYY DNQQKVVGYS QDYSNAIVEA VKKKLNKPDL QVKLIPITSQ NRIPLLQNGT FDFECGSTTN NVERQKQAAF SDTIFVVGTR LLTKKGGDIK DFANLKDKAV VVTSGTTSEV LLNKLNEEQK MNMRIISAKD HGDSFRTLES GRAVAFMMDD ALLAGERAKA KKPDNWEIVG KPQSQEAYGC MLRKDDPQFK KLMDDTIAQV QTSGEAEKWF DKWFKNPIPP KNLNMNFELS DEMKALFKEP NDKALN |
-実験情報
ビーム | 設備名称: ESRF BM29 / 地域: Grenoble / 国: France ![]() | ||||||||||||||||||||||||||||||
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検出器 | 名称: Pilatus 1M / タイプ: Dectris / Pixsize x: 172 mm | ||||||||||||||||||||||||||||||
スキャン | 測定日: 2018年9月10日 / セル温度: 20 °C / 照射時間: 2 sec. / フレーム数: 12 / 単位: 1/nm /
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距離分布関数 P(R) |
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結果 | コメント: Glu-bound DEBP at a ligand:protein ratio of 10:1. Ligand condition is prepared by dissolving glutamate in powder form (Sigma-Aldrich) directly into the SAXS buffer, followed by dilution ...コメント: Glu-bound DEBP at a ligand:protein ratio of 10:1. Ligand condition is prepared by dissolving glutamate in powder form (Sigma-Aldrich) directly into the SAXS buffer, followed by dilution to the target concentration with additional SAXS buffer. Note that the scattering intensity therefore contains Na+ counterions that was not directly subtracted.
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