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Open data
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Basic information
Entry | ![]() |
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![]() | Histidine-binding periplasmic protein (HisBP), apo-form
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Function / homology | ![]() L-histidine import across plasma membrane / amino acid binding / ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing / outer membrane-bounded periplasmic space / membrane Similarity search - Function |
Biological species | ![]() ![]() |
![]() | ![]() Title: Structure-based screening of binding affinities via small-angle X-ray scattering Authors: Chen P / Masiewicz P / Perez K |
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Structure visualization
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Downloads & links
-Models
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Sample
![]() | Name: Histidine-binding periplasmic protein (HisBP), apo-form Specimen concentration: 4.25 mg/ml |
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Buffer | Name: 100 mM NaCl, 20 mM NaPO4, 0.5 mM TCEP / pH: 7.4 / Comment: SEC buffer for PBP experiments |
Entity #1753 | Name: HisBP / Type: protein / Description: Histidine-binding periplasmic protein / Formula weight: 26.289 / Num. of mol.: 1 / Source: Escherichia coli (strain K12) / References: UniProt: P0AEU0 Sequence: GAIPQNIRIG TDPTYAPFES KNSQGELVGF DIDLAKELCK RINTQCTFVE NPLDALIPSL KAKKIDAIMS SLSITEKRQQ EIAFTDKLYA ADSRLVVAKN SDIQPTVESL KGKRVGVLQG TTQETFGNEH WAPKGIEIVS YQGQDNIYSD LTAGRIDAAF QDEVAASEGF ...Sequence: GAIPQNIRIG TDPTYAPFES KNSQGELVGF DIDLAKELCK RINTQCTFVE NPLDALIPSL KAKKIDAIMS SLSITEKRQQ EIAFTDKLYA ADSRLVVAKN SDIQPTVESL KGKRVGVLQG TTQETFGNEH WAPKGIEIVS YQGQDNIYSD LTAGRIDAAF QDEVAASEGF LKQPVGKDYK FGGPSVKDEK LFGVGTGMGL RKEDNELREA LNKAFAEMRA DGTYEKLAKK YFDFDVYGG |
-Experimental information
Beam | Instrument name: ESRF BM29 / City: Grenoble / 国: France ![]() | |||||||||||||||||||||||||||||||||
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Detector | Name: Pilatus 1M / Type: Dectris / Pixsize x: 172 mm | |||||||||||||||||||||||||||||||||
Scan | Measurement date: Sep 10, 2018 / Cell temperature: 20 °C / Exposure time: 2 sec. / Number of frames: 12 / Unit: 1/nm /
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Distance distribution function P(R) |
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Result | Comments: Free HisBP after TEV cleavage to remove hexahistidine tags, followed by reverse His-Trap and SEC column purification. Note N-terminal glycine before the Uniprot sequence due to TEV tag.
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