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- SASDFF8: Glutamine-binding periplasmic protein with hexahistidine tag (Gln... -
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Open data
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Basic information
Entry | ![]() |
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![]() | Glutamine-binding periplasmic protein with hexahistidine tag (GlnBP), apo-form
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Function / homology | ![]() glutamine binding / L-glutamine import across plasma membrane / ligand-gated monoatomic ion channel activity / ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing / outer membrane-bounded periplasmic space / membrane Similarity search - Function |
Biological species | ![]() ![]() |
![]() | ![]() Title: Structure-based screening of binding affinities via small-angle X-ray scattering Authors: Chen P / Masiewicz P / Perez K |
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Structure visualization
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Downloads & links
-Data source
SASBDB page | ![]() |
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-Related structure data
Related structure data | C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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External links
Related items in Molecule of the Month |
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-Models
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Sample
![]() | Name: Glutamine-binding periplasmic protein with hexahistidine tag (GlnBP), apo-form Specimen concentration: 2 mg/ml |
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Buffer | Name: 100 mM NaCl, 20 mM NaPO4, 0.5 mM TCEP / pH: 7.4 / Comment: SEC buffer for PBP experiments |
Entity #1754 | Name: GlnBP / Type: protein Description: Glutamine-binding periplasmic protein with hexahistidine tag Formula weight: 25.786 / Num. of mol.: 1 / Source: Escherichia coli (strain K12) / References: UniProt: P0AEQ3 Sequence: HHHHHHADKK LVVATDTAFV PFEFKQGDKY VGFDVDLWAA IAKELKLDYE LKPMDFSGII PALQTKNVDL ALAGITITDE RKKAIDFSDG YYKSGLLVMV KANNNDVKSV KDLDGKVVAV KSGTGSVDYA KANIKTKDLR QFPNIDNAYM ELGTNRADAV LHDTPNILYF ...Sequence: HHHHHHADKK LVVATDTAFV PFEFKQGDKY VGFDVDLWAA IAKELKLDYE LKPMDFSGII PALQTKNVDL ALAGITITDE RKKAIDFSDG YYKSGLLVMV KANNNDVKSV KDLDGKVVAV KSGTGSVDYA KANIKTKDLR QFPNIDNAYM ELGTNRADAV LHDTPNILYF IKTAGNGQFK AVGDSLEAQQ YGIAFPKGSD ELRDKVNGAL KTLRENGTYN EIYKKWFGTE PK |
-Experimental information
Beam | Instrument name: ESRF BM29 / City: Grenoble / 国: France ![]() | |||||||||||||||||||||||||||||||||
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Detector | Name: Pilatus 1M / Type: Dectris / Pixsize x: 172 mm | |||||||||||||||||||||||||||||||||
Scan | Measurement date: Sep 10, 2018 / Cell temperature: 20 °C / Exposure time: 2 sec. / Number of frames: 12 / Unit: 1/nm /
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Distance distribution function P(R) |
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Result | Comments: Free hexahistidine-tagged GlnBP after His-Trap and SEC column purification. Sequence assumed based on Clifton et al. 2016, which reports a plasmid containing a direct attachment of N-terminal His tag.
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