SASDFE8: Histidine-binding periplasmic protein (HisBP) in the presence of ...

Basic information

Entry

Database: SASBDB / ID: SASDFE8

Sample

Histidine-binding periplasmic protein (HisBP) in the presence of histidine - His-bound 5-fold excess

• Histidine-binding periplasmic protein (protein), HisBP, Escherichia coli (strain K12)

Function / homology

Function:

L-histidine import across plasma membrane / amino acid binding / ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing / outer membrane-bounded periplasmic space / membrane

Domain/homology:

Specific amino acids and opine-binding periplasmic protein, ABC transporter / Solute-binding protein family 3, conserved site / Bacterial extracellular solute-binding proteins, family 3 signature. / Bacterial periplasmic substrate-binding proteins / Bacterial extracellular solute-binding proteins, family 3 / Solute-binding protein family 3/N-terminal domain of MltF

Component:

Histidine-binding periplasmic protein

• Biological species: Escherichia coli (strain K12) (bacteria)
• Citation: Date: 2019 Aug 1; Title: Structure-based screening of binding affinities via small-angle X-ray scattering; Authors: Chen P / Masiewicz P / Perez K

Contact author

• Po-chia Chen (EMBL, European Molecular Biology Laboratory (EMBL) - Heidelberg, Heidelberg, Germany)

Models

Sample

• Sample: Name: Histidine-binding periplasmic protein (HisBP) in the presence of histidine - His-bound 5-fold excess; Specimen concentration: 4.2 mg/ml
• Buffer: Name: 100 mM NaCl, 20 mM NaPO4, 0.5 mM TCEP / pH: 7.4 / Comment: SEC buffer for PBP experiments

Entity #1753

Name: HisBP / Type: protein / Description: Histidine-binding periplasmic protein / Formula weight: 26.289 / Num. of mol.: 1 / Source: Escherichia coli (strain K12) / References: UniProt: P0AEU0
Sequence:

GAIPQNIRIG TDPTYAPFES KNSQGELVGF DIDLAKELCK RINTQCTFVE NPLDALIPSL KAKKIDAIMS SLSITEKRQQ EIAFTDKLYA ADSRLVVAKN SDIQPTVESL KGKRVGVLQG TTQETFGNEH WAPKGIEIVS YQGQDNIYSD LTAGRIDAAF QDEVAASEGF LKQPVGKDYK FGGPSVKDEK LFGVGTGMGL RKEDNELREA LNKAFAEMRA DGTYEKLAKK YFDFDVYGG

Experimental information

• Beam: Instrument name: ESRF BM29 / City: Grenoble / 国: France / Type of source: X-ray synchrotron / Wavelength: 0.09919 Å / Dist. spec. to detc.: 2.849 mm
• Detector: Name: Pilatus 1M / Type: Dectris / Pixsize x: 172 mm

Scan

Title: Histidine-binding periplasmic protein (HisBP) in the presence of histidine - His-bound 5-fold excess
Measurement date: Sep 10, 2018 / Cell temperature: 20 °C / Exposure time: 2 sec. / Number of frames: 12 / Unit: 1/nm /

	Min	Max
Q	0.0306	4.9462

Distance distribution function P(R)

Sofotware P(R): GNOM 5.0 / Number of points: 573 /

	Min	Max
Q	0.101354	2.79976
P(R) point	1	573
R	0	5.71

Result

Type of curve: single_conc
Comments: His-bound HisBP at a ligand:protein ratio of 5:1. Ligand condition is prepared by dissolving histidine in powder form (Sigma-Aldrich) directly into the SAXS buffer, followed by dilution to the target concentration with additional SAXS buffer.

	Experimental	Porod
MW	30 kDa	21 kDa
Volume	-	33 nm3

	P(R)	Guinier	Guinier error
Forward scattering, I0	128.8	128.71	0.1
Radius of gyration, Rg	1.833 nm	1.83 nm	-

	Min	Max
D	-	5.71
Guinier point	12	144