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Yorodumi- SASDFH8: Glutamate/aspartate import solute-binding protein (DEBP), apo-form -
+Open data
-Basic information
Entry | Database: SASBDB / ID: SASDFH8 |
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Sample | Glutamate/aspartate import solute-binding protein (DEBP), apo-form
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Function / homology | Function and homology information aspartate binding / L-glutamate transmembrane transport / L-aspartate transmembrane transport / L-aspartate import across plasma membrane / L-glutamate import across plasma membrane / glutamate binding / ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing / outer membrane-bounded periplasmic space / membrane Similarity search - Function |
Biological species | Escherichia coli (strain K12) (bacteria) |
Citation | Date: 2019 Aug 1 Title: Structure-based screening of binding affinities via small-angle X-ray scattering Authors: Chen P / Masiewicz P / Perez K |
Contact author |
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-Structure visualization
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-Models
-Sample
Sample | Name: Glutamate/aspartate import solute-binding protein (DEBP), apo-form Specimen concentration: 2.2 mg/ml |
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Buffer | Name: 100 mM NaCl, 20 mM NaPO4, 0.5 mM TCEP / pH: 7.4 / Comment: SEC buffer for PBP experiments |
Entity #1755 | Type: protein Description: Glutamate/aspartate import solute-binding protein Formula weight: 32.052 / Num. of mol.: 1 / Source: Escherichia coli (strain K12) / References: UniProt: P37902 Sequence: HHHHHHDDAA PAAGSTLDKI AKNGVIVVGH RESSVPFSYY DNQQKVVGYS QDYSNAIVEA VKKKLNKPDL QVKLIPITSQ NRIPLLQNGT FDFECGSTTN NVERQKQAAF SDTIFVVGTR LLTKKGGDIK DFANLKDKAV VVTSGTTSEV LLNKLNEEQK MNMRIISAKD ...Sequence: HHHHHHDDAA PAAGSTLDKI AKNGVIVVGH RESSVPFSYY DNQQKVVGYS QDYSNAIVEA VKKKLNKPDL QVKLIPITSQ NRIPLLQNGT FDFECGSTTN NVERQKQAAF SDTIFVVGTR LLTKKGGDIK DFANLKDKAV VVTSGTTSEV LLNKLNEEQK MNMRIISAKD HGDSFRTLES GRAVAFMMDD ALLAGERAKA KKPDNWEIVG KPQSQEAYGC MLRKDDPQFK KLMDDTIAQV QTSGEAEKWF DKWFKNPIPP KNLNMNFELS DEMKALFKEP NDKALN |
-Experimental information
Beam | Instrument name: ESRF BM29 / City: Grenoble / 国: France / Type of source: X-ray synchrotron / Wavelength: 0.09919 Å / Dist. spec. to detc.: 2.849 mm | |||||||||||||||||||||||||||||||||
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Detector | Name: Pilatus 1M / Type: Dectris / Pixsize x: 172 mm | |||||||||||||||||||||||||||||||||
Scan | Title: Glutamate/aspartate import solute-binding protein (DEBP), apo-form Measurement date: Sep 10, 2018 / Cell temperature: 20 °C / Exposure time: 2 sec. / Number of frames: 12 / Unit: 1/nm /
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Distance distribution function P(R) | Sofotware P(R): GNOM 5.0 / Number of points: 573 /
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Result | Type of curve: single_conc Comments: Free hexahistidine-tagged DEBP after His-Trap and SEC column purification. Sequence assumed based on Clifton et al. 2016, which reports a plasmid containing a direct attachment of N-terminal His tag.
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