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- SASDFJ8: Glutamate/aspartate import solute-binding protein (DEBP) in the p... -
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Open data
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Basic information
Entry | ![]() |
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![]() | Glutamate/aspartate import solute-binding protein (DEBP) in the presence of glutamate - Glu-bound 10-fold excess
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Function / homology | ![]() aspartate binding / L-glutamate transmembrane transport / L-aspartate transmembrane transport / L-aspartate import across plasma membrane / L-glutamate import across plasma membrane / glutamate binding / ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing / outer membrane-bounded periplasmic space / membrane Similarity search - Function |
Biological species | ![]() ![]() |
![]() | ![]() Title: Structure-based screening of binding affinities via small-angle X-ray scattering Authors: Chen P / Masiewicz P / Perez K |
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Structure visualization
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Downloads & links
-Models
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Sample
![]() | Name: Glutamate/aspartate import solute-binding protein (DEBP) in the presence of glutamate - Glu-bound 10-fold excess Specimen concentration: 2.2 mg/ml |
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Buffer | Name: 100 mM NaCl, 20 mM NaPO4, 0.5 mM TCEP / pH: 7.4 / Comment: SEC buffer for PBP experiments |
Entity #1755 | Type: protein Description: Glutamate/aspartate import solute-binding protein Formula weight: 32.052 / Num. of mol.: 1 / Source: Escherichia coli (strain K12) / References: UniProt: P37902 Sequence: HHHHHHDDAA PAAGSTLDKI AKNGVIVVGH RESSVPFSYY DNQQKVVGYS QDYSNAIVEA VKKKLNKPDL QVKLIPITSQ NRIPLLQNGT FDFECGSTTN NVERQKQAAF SDTIFVVGTR LLTKKGGDIK DFANLKDKAV VVTSGTTSEV LLNKLNEEQK MNMRIISAKD ...Sequence: HHHHHHDDAA PAAGSTLDKI AKNGVIVVGH RESSVPFSYY DNQQKVVGYS QDYSNAIVEA VKKKLNKPDL QVKLIPITSQ NRIPLLQNGT FDFECGSTTN NVERQKQAAF SDTIFVVGTR LLTKKGGDIK DFANLKDKAV VVTSGTTSEV LLNKLNEEQK MNMRIISAKD HGDSFRTLES GRAVAFMMDD ALLAGERAKA KKPDNWEIVG KPQSQEAYGC MLRKDDPQFK KLMDDTIAQV QTSGEAEKWF DKWFKNPIPP KNLNMNFELS DEMKALFKEP NDKALN |
-Experimental information
Beam | Instrument name: ESRF BM29 / City: Grenoble / 国: France ![]() | ||||||||||||||||||||||||||||||
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Detector | Name: Pilatus 1M / Type: Dectris / Pixsize x: 172 mm | ||||||||||||||||||||||||||||||
Scan | Measurement date: Sep 10, 2018 / Cell temperature: 20 °C / Exposure time: 2 sec. / Number of frames: 12 / Unit: 1/nm /
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Distance distribution function P(R) |
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Result | Comments: Glu-bound DEBP at a ligand:protein ratio of 10:1. Ligand condition is prepared by dissolving glutamate in powder form (Sigma-Aldrich) directly into the SAXS buffer, followed by dilution to ...Comments: Glu-bound DEBP at a ligand:protein ratio of 10:1. Ligand condition is prepared by dissolving glutamate in powder form (Sigma-Aldrich) directly into the SAXS buffer, followed by dilution to the target concentration with additional SAXS buffer. Note that the scattering intensity therefore contains Na+ counterions that was not directly subtracted.
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