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- SASDEK6: Complex of Epstein-Barr nuclear antigen 2 (EBNA2 Type2, amino aci... -

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Basic information

Entry
Database: SASBDB / ID: SASDEK6
SampleComplex of Epstein-Barr nuclear antigen 2 (EBNA2 Type2, amino acids 348-422) and BS69 (Zinc finger MYND domain-containing protein 11: CC-MYND, amino acids 480-602)
  • Epstein-Barr nuclear antigen 2 (protein), EBNA2 Type2, Epstein-Barr virus (strain AG876)
  • Zinc finger MYND domain-containing protein 11 (protein), BS69 CC-MYND, Homo sapiens
Function / homology: / : / host cell nuclear matrix / symbiont-mediated suppression of host type I interferon-mediated signaling pathway / Isoform 1 of Zinc finger MYND domain-containing protein 11 / Epstein-Barr nuclear antigen 2
Function and homology information
Biological speciesEpstein-Barr virus (strain AG876)
Homo sapiens (human)
CitationDate: 2018 Nov 6
Title: Increased association between Epstein-Barr virus EBNA2 from type 2 strains and the transcriptional repressor BS69 restricts B cell growth
Authors: Ponnusamy R / Khatri R / Correia P / Mancini E / Farrell P
Contact author
  • Rajesh Ponnusamy (University of Sussex, Brighton, UK)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #2543
Type: dummy / Radius of dummy atoms: 4.25 A / Chi-square value: 1.001 / P-value: 0.493446
Search similar-shape structures of this assembly by Omokage search (details)
Model #2550
Type: dummy / Radius of dummy atoms: 4.25 A / Chi-square value: 1.001 / P-value: 0.493446
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Complex of Epstein-Barr nuclear antigen 2 (EBNA2 Type2, amino acids 348-422) and BS69 (Zinc finger MYND domain-containing protein 11: CC-MYND, amino acids 480-602)
Specimen concentration: 9 mg/ml / Entity id: 1342 / 1345
BufferName: 20mM Tris-HCl, 100mM NaCl, 2% Sucrose and 1mM TCEP / pH: 7.5
Entity #1342Name: EBNA2 Type2 / Type: protein / Description: Epstein-Barr nuclear antigen 2 / Formula weight: 7.934 / Num. of mol.: 2 / Source: Epstein-Barr virus (strain AG876) / References: UniProt: Q69022
Sequence:
SMPQLSPVVS LHQGQGPENS PTPGPSTAGP VCRVTPSATP DISPIHEPES SDSEEPPFLF PSDWYPPTLE PAELD
Entity #1345Name: BS69 CC-MYND / Type: protein / Description: Zinc finger MYND domain-containing protein 11 / Formula weight: 15.064 / Num. of mol.: 6 / Source: Homo sapiens / References: UniProt: Q15326-1
Sequence:
DRMKSDHKRE TERVVREALE KLRSEMEEEK RQAVNKAVAN MQGEMDRKCK QVKEKCKEEF VEEIKKLATQ HKQLISQTKK KQWCYNCEEE AMYHCCWNTS YCSIKCQQEH WHAEHKRTCR RKR

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Experimental information

BeamInstrument name: Diamond Light Source B21 / City: Didcot / 国: UK / Shape: 1 x 5 mm / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.1 Å / Dist. spec. to detc.: 4.014 mm
DetectorName: Pilatus 2M
Scan
Title: Complex of Epstein-Barr nuclear antigen 2 (EBNA2 Type2, amino acids 348-422) and BS69 (Zinc finger MYND domain-containing protein 11: CC-MYND, amino acids 480-602)
Measurement date: Sep 23, 2017 / Storage temperature: 4 °C / Cell temperature: 25 °C / Exposure time: 3 sec. / Number of frames: 33 / Unit: 1/A /
MinMax
Q0.0156 0.2321
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 698 /
MinMax
Q0.015574 0.17047
P(R) point1 698
R0 145.4
Result
Type of curve: sec
Comments: SEC-SAXS was performed using the following parameters: Column type: S200 3.2/300 increase; Flow rate: 0.075 ml/min; Injection volume: 45 µl; Sample injection concentration: 9.0 mg/ml. The ...Comments: SEC-SAXS was performed using the following parameters: Column type: S200 3.2/300 increase; Flow rate: 0.075 ml/min; Injection volume: 45 µl; Sample injection concentration: 9.0 mg/ml. The molar ratio of the injected sample components was 1:3 (EBNA2:BS69). The final complex contains six BS69 CC-MYND (3 dimers) with two EBNA2 Type2 (348-422) polypeptides.
ExperimentalPorod
MW95.3 kDa-
Volume-239.07 nm3

P(R)GuinierGuinier error
Forward scattering, I00.008248 0.008297 8.0E-5
Radius of gyration, Rg4.82 nm4.68 nm0.6

MinMax
D-14.54
Guinier point1 55

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