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- SASDEJ6: Complex of Epstein-Barr nuclear antigen 2 (EBNA2 Type1, amino aci... -

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Basic information

Entry
Database: SASBDB / ID: SASDEJ6
SampleComplex of Epstein-Barr nuclear antigen 2 (EBNA2 Type1, amino acids 381-455) and BS69 (Zinc finger MYND domain-containing protein 11: CC-MYND, amino acids 480-602)
  • Epstein-Barr nuclear antigen 2 (protein), EBNA2 Type1, Epstein-Barr virus (strain B95-8)
  • Zinc finger MYND domain-containing protein 11 (protein), BS69 CC-MYND, Homo sapiens
Function / homology
Function and homology information


symbiont-mediated perturbation of host cell cycle progression / host cell nuclear matrix / symbiont-mediated perturbation of host transcription / DNA-templated viral transcription / protein serine/threonine phosphatase inhibitor activity / symbiont-mediated suppression of host translation initiation / symbiont-mediated suppression of host type I interferon-mediated signaling pathway / virus-mediated perturbation of host defense response / host cell nucleus / positive regulation of DNA-templated transcription
Similarity search - Function
Epstein-Barr nuclear antigen 2 / Isoform 1 of Zinc finger MYND domain-containing protein 11
Similarity search - Component
Biological speciesEpstein-Barr virus (strain B95-8) (Epstein-Barr virus (strain B95-8))
Homo sapiens (human)
CitationDate: 2018 Nov 6
Title: Increased association between Epstein-Barr virus EBNA2 from type 2 strains and the transcriptional repressor BS69 restricts B cell growth
Authors: Ponnusamy R / Khatri R / Correia P / Mancini E / Farrell P
Contact author
  • Rajesh Ponnusamy (University of Sussex, Brighton, UK)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #2541
Type: dummy / Radius of dummy atoms: 3.00 A / Chi-square value: 1.400 / P-value: 0.000312
Search similar-shape structures of this assembly by Omokage search (details)
Model #2549
Type: dummy / Radius of dummy atoms: 3.00 A / Chi-square value: 1.400 / P-value: 0.000312
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Complex of Epstein-Barr nuclear antigen 2 (EBNA2 Type1, amino acids 381-455) and BS69 (Zinc finger MYND domain-containing protein 11: CC-MYND, amino acids 480-602)
Specimen concentration: 9 mg/ml / Entity id: 1341 / 1343
BufferName: 20mM Tris-HCl, 100mM NaCl, 2% Sucrose and 1mM TCEP / pH: 7.5
Entity #1341Name: EBNA2 Type1 / Type: protein / Description: Epstein-Barr nuclear antigen 2 / Formula weight: 7.831 / Num. of mol.: 2 / Source: Epstein-Barr virus (strain B95-8) / References: UniProt: P12978
Sequence:
SMPELSPVLG LHQGQGAGDS PTPGPSNAAP VCRNSHTATP NVSPIHEPES HNSPEAPILF PDDWYPPSID PADLD
Entity #1343Name: BS69 CC-MYND / Type: protein / Description: Zinc finger MYND domain-containing protein 11 / Formula weight: 15.064 / Num. of mol.: 4 / Source: Homo sapiens / References: UniProt: Q15326-1
Sequence:
DRMKSDHKRE TERVVREALE KLRSEMEEEK RQAVNKAVAN MQGEMDRKCK QVKEKCKEEF VEEIKKLATQ HKQLISQTKK KQWCYNCEEE AMYHCCWNTS YCSIKCQQEH WHAEHKRTCR RKR

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Experimental information

BeamInstrument name: Diamond Light Source B21 / City: Didcot / 国: UK / Shape: 1 x 5 mm / Type of source: X-ray synchrotron / Wavelength: 0.1 Å / Dist. spec. to detc.: 4.014 mm
DetectorName: Pilatus 2M
Scan
Title: Complex of Epstein-Barr nuclear antigen 2 (EBNA2 Type1, amino acids 381-455) and BS69 (Zinc finger MYND domain-containing protein 11: CC-MYND, amino acids 480-602)
Measurement date: Nov 29, 2017 / Storage temperature: 4 °C / Cell temperature: 25 °C / Exposure time: 3 sec. / Number of frames: 25 / Unit: 1/A /
MinMax
Q0.0103 0.2417
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 674 /
MinMax
Q0.0102666 0.159107
P(R) point1 674
R0 138.3
Result
Type of curve: sec
Comments: SEC-SAXS was performed using the following parameters: Column type: S200 3.2/300 increase; Flow rate: 0.075 ml/min; Injection volume: 45 µl; Sample injection concentration: 9.0 mg/ml. The ...Comments: SEC-SAXS was performed using the following parameters: Column type: S200 3.2/300 increase; Flow rate: 0.075 ml/min; Injection volume: 45 µl; Sample injection concentration: 9.0 mg/ml. The molar ratio of the injected sample components was 1:3 (EBNA2:BS69). The final complex contains four BS69 CC-MYND (2 dimers) with two EBNA2 Type1 (381-455) polypeptides.
ExperimentalPorod
MW69.4 kDa-
Volume-72 nm3

P(R)GuinierGuinier error
Forward scattering, I00.01062 0.0107 0.0001
Radius of gyration, Rg4.65 nm4.57 nm0.08

MinMax
D-13.83
Guinier point29 84

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