+
データを開く
-
基本情報
登録情報 | データベース: SASBDB / ID: SASDDD3 |
---|---|
![]() | Human mitochondrial cysteine desulfurase-ISCU-Frataxin (NFS1, ISD11 and Acp heterodimer complex)
|
機能・相同性 | ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() 類似検索 - 分子機能 |
生物種 | ![]() ![]() ![]() ![]() ![]() |
![]() | ![]() タイトル: Architectural Features of Human Mitochondrial Cysteine Desulfurase Complexes from Crosslinking Mass Spectrometry and Small-Angle X-Ray Scattering. 著者: Kai Cai / Ronnie O Frederick / Hesam Dashti / John L Markley / ![]() 要旨: Cysteine desulfurase plays a central role in mitochondrial iron-sulfur cluster biogenesis by generating sulfur through the conversion of L-cysteine to L-alanine and by serving as the platform for ...Cysteine desulfurase plays a central role in mitochondrial iron-sulfur cluster biogenesis by generating sulfur through the conversion of L-cysteine to L-alanine and by serving as the platform for assembling other components of the biosynthetic machinery, including ISCU, frataxin, and ferredoxin. The human mitochondrial cysteine desulfurase complex consists of two copies each of NFS1, ISD11, and acyl carrier protein. We describe results from chemical crosslinking coupled with tandem mass spectrometry and small-angle X-ray scattering studies that are consistent with a closed NFS1 dimer rather than an open one for both the cysteine desulfurase-ISCU and cysteine desulfurase-ISCU-frataxin complexes. We present a structural model for the cysteine desulfurase-ISCU-frataxin complex derived from chemical crosslinking restraints in conjunction with the recent crystal structure of the cysteine desulfurase-ISCU-zinc complex and distance constraints from nuclear magnetic resonance. |
![]() |
|
-
構造の表示
構造ビューア | 分子: ![]() ![]() |
---|
-
ダウンロードとリンク
-モデル
モデル #1776 | ![]() タイプ: atomic / ダミー原子の半径: 1.90 A / カイ2乗値: 4.937284 / P-value: 0.000004 ![]() |
---|
-
試料
![]() | 名称: Human mitochondrial cysteine desulfurase-ISCU-Frataxin (NFS1, ISD11 and Acp heterodimer complex) 試料濃度: 2.00-10.00 / Entity id: 958 / 959 / 960 / 961 / 962 |
---|---|
バッファ | 名称: 20 mM HEPES, 150 mM NaCl, 5 mM TCEP / pH: 7.5 / コメント: HNT Buffer |
要素 #958 | 名称: NFS1 / タイプ: protein / 記述: Cysteine desulfurase, mitochondrial![]() 配列: GPVLRPLYMD VQATTPLDPR VLDAMLPYLI NYYGNPHSRT HAYGWESEAA MERARQQVAS LIGADPREII FTSGATESNN IAIKGVARFY RSRKKHLITT QTEHKCVLDS CRSLEAEGFQ VTYLPVQKSG IIDLKELEAA IQPDTSLVSV MTVNNEIGVK QPIAEIGRIC ...配列: GPVLRPLYMD VQATTPLDPR VLDAMLPYLI NYYGNPHSRT HAYGWESEAA MERARQQVAS LIGADPREII FTSGATESNN IAIKGVARFY RSRKKHLITT QTEHKCVLDS CRSLEAEGFQ VTYLPVQKSG IIDLKELEAA IQPDTSLVSV MTVNNEIGVK QPIAEIGRIC SSRKVYFHTD AAQAVGKIPL DVNDMKIDLM SISGHKIYGP KGVGAIYIRR RPRVRVEALQ SGGGQERGMR SGTVPTPLVV GLGAACEVAQ QEMEYDHKRI SKLSERLIQN IMKSLPDVVM NGDPKHHYPG CINLSFAYVE GESLLMALKD VALSSGSACT SASLEPSYVL RAIGTDEDLA HSSIRFGIGR FTTEEEVDYT VEKCIQHVKR LREMSPLWEM VQDGIDLKSI KWTQH |
要素 #959 | 名称: ISD11 / タイプ: protein / 記述: LYR motif-containing protein 4 / 分子量: 11.581 / 分子数: 2 / 由来: Homo sapiens / 参照: UniProt: Q9HD34 配列: MAASSRAQVL SLYRAMLRES KRFSAYNYRT YAVRRIRDAF RENKNVKDPV EIQTLVNKAK RDLGVIRRQV HIGQLYSTDK LIIENRDMPR THHHHHH |
要素 #960 | タイプ: protein / 記述: Acyl carrier protein![]() 配列: PspP0A6A8A CPECOLIAcy lcarrierpr oteinOSEsc herichiaco li(strainK12)GNacpPP E1SV2MSTIE ERVKKIIGEQ LGVKQEEVTN NASFVEDLGA DSLDTVELVM ALEEEFDTEI PDEEAEKITT VQAAIDYING HQA |
要素 #961 | 名称: ISCU![]() 記述: Iron-sulfur cluster assembly enzyme ISCU, mitochondrial 分子量: 14.655 / 分子数: 2 / 由来: Homo sapiens / 参照: UniProt: Q9H1K1 配列: RLYHKKVVDH YENPRNVGSL DKTSKNVGTG LVGAPACGDV MKLQIQVDEK GKIVDARFKT FGCGSAIASS SLATEWVKGK TVEEALTIKN TDIAKELCLP PVKLHCSMLA EDAIKAALAD YKLKQEPKKG EAEKK |
要素 #962 | 名称: FXN / タイプ: protein / 記述: Frataxin, mitochondrial![]() 配列: SGTLGHPGSL DETTYERLAE ETLDSLAEFF EDLADKPYTF EDYDVSFGSG VLTVKLGGDL GTYVINKQTP NKQIWLSSPS SGPKRYDWTG KNWVYSHDGV SLHELLAAEL TKALKTKLDL SSLAYSGKDA |
-実験情報
ビーム | 設備名称: NMRFAM Bruker Nanostar / 地域: Madison, WI / 国: USA ![]() ![]() | ||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
検出器 | 名称: VÅNTEC-2000 / タイプ: MikroGap / Pixsize x: 200 mm | ||||||||||||||||||||||||||||||||||||
スキャン | 測定日: 2017年4月18日 / 保管温度: 25 °C / セル温度: 25 °C / 照射時間: 3600 sec. / フレーム数: 4 / 単位: 1/A /
| ||||||||||||||||||||||||||||||||||||
距離分布関数 P(R) |
| ||||||||||||||||||||||||||||||||||||
結果 | コメント: The solution SAXS study of cysteine desulfurase complex containing ISCU and Frataxin
|