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- PDB-5xox: Crystal structure of tRNA(His) guanylyltranserase from Saccharomy... -

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Basic information

Entry
Database: PDB / ID: 5xox
TitleCrystal structure of tRNA(His) guanylyltranserase from Saccharomyces cerevisiae
ComponentstRNA(His) guanylyltransferase
KeywordsTRANSFERASE / Thg1 / reverse polymerization / post-transcriptional modification / GTP
Function / homology
Function and homology information


tRNAHis guanylyltransferase / tRNA guanylyltransferase activity / tRNA modification / tRNA processing / GTP binding / magnesium ion binding / identical protein binding / nucleus / cytoplasm
Similarity search - Function
Thg1 C-terminal domain / Thg1 C terminal domain / tRNAHis guanylyltransferase Thg1 / tRNAHis guanylyltransferase catalytic domain / tRNAHis guanylyltransferase Thg1 superfamily / tRNAHis guanylyltransferase / tRNA(His) guanylyltransferase (Thg1) / Alpha-Beta Plaits / 2-Layer Sandwich / Alpha Beta
Similarity search - Domain/homology
GUANOSINE-5'-TRIPHOSPHATE / PHOSPHATE ION / tRNA(His) guanylyltransferase
Similarity search - Component
Biological speciesSaccharomyces cerevisiae (brewer's yeast)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3 Å
AuthorsLee, K. / Lee, E.H. / Son, J. / Hwang, K.Y.
Funding support Korea, Republic Of, 1items
OrganizationGrant numberCountry
National Research Foundation of Korea2017R1A2B2005666 Korea, Republic Of
CitationJournal: Biochem. Biophys. Res. Commun. / Year: 2017
Title: Crystal structure of tRNA(His) guanylyltransferase from Saccharomyces cerevisiae
Authors: Lee, K. / Lee, E.H. / Son, J. / Hwang, K.Y.
History
DepositionMay 31, 2017Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Jul 12, 2017Provider: repository / Type: Initial release
Revision 1.1Jul 26, 2017Group: Database references / Category: citation
Item: _citation.journal_volume / _citation.page_first / _citation.page_last
Revision 1.2Aug 16, 2017Group: Database references / Category: pdbx_related_exp_data_set
Revision 1.3Nov 22, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_conn
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_conn.pdbx_dist_value / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: tRNA(His) guanylyltransferase
B: tRNA(His) guanylyltransferase
C: tRNA(His) guanylyltransferase
D: tRNA(His) guanylyltransferase
E: tRNA(His) guanylyltransferase
F: tRNA(His) guanylyltransferase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)175,05826
Polymers171,7256
Non-polymers3,33420
Water00
1
A: tRNA(His) guanylyltransferase
B: tRNA(His) guanylyltransferase
C: tRNA(His) guanylyltransferase
D: tRNA(His) guanylyltransferase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)117,15020
Polymers114,4834
Non-polymers2,66716
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area15640 Å2
ΔGint-166 kcal/mol
Surface area41690 Å2
MethodPISA
2
E: tRNA(His) guanylyltransferase
F: tRNA(His) guanylyltransferase
hetero molecules

E: tRNA(His) guanylyltransferase
F: tRNA(His) guanylyltransferase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)115,81712
Polymers114,4834
Non-polymers1,3348
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
crystal symmetry operation6_445-x-1,-y-1,z1
Buried area10640 Å2
ΔGint-118 kcal/mol
Surface area34280 Å2
MethodPISA
Unit cell
Length a, b, c (Å)164.703, 164.703, 275.891
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number80
Space group name H-MI41

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Components

#1: Protein
tRNA(His) guanylyltransferase / tRNA-histidine guanylyltransferase


Mass: 28620.775 Da / Num. of mol.: 6 / Fragment: UNP residues 5-233
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c / Gene: THG1, YGR024C / Production host: Escherichia coli (E. coli) / References: UniProt: P53215, tRNAHis guanylyltransferase
#2: Chemical
ChemComp-GTP / GUANOSINE-5'-TRIPHOSPHATE


Mass: 523.180 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: C10H16N5O14P3 / Comment: GTP, energy-carrying molecule*YM
#3: Chemical
ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 10 / Source method: obtained synthetically / Formula: Mg
#4: Chemical
ChemComp-PO4 / PHOSPHATE ION


Mass: 94.971 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: PO4

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 5.45 Å3/Da / Density % sol: 77.42 %
Crystal growTemperature: 277 K / Method: vapor diffusion, hanging drop / Details: 0.1M MES pH 6.0, 0.9M Ammonium sulfate

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: Photon Factory / Beamline: AR-NW12A / Wavelength: 0.97952 Å
DetectorType: ADSC QUANTUM 270 / Detector: CCD / Date: Dec 4, 2012
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97952 Å / Relative weight: 1
ReflectionResolution: 3→29.841 Å / Num. obs: 68293 / % possible obs: 93.42 % / Redundancy: 5.1 % / Rmerge(I) obs: 0.1419 / Net I/σ(I): 17.87
Reflection shellResolution: 3→3.107 Å / Redundancy: 2.1 % / Rmerge(I) obs: 0.3766 / Mean I/σ(I) obs: 3.02 / Num. unique obs: 12656 / % possible all: 84

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Processing

Software
NameVersionClassification
PHENIX(1.10.1_2155: ???)refinement
HKL-2000data reduction
HKL-2000data scaling
PHENIXphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 3otb

3otb


Resolution: 3→29.841 Å / SU ML: 0.63 / Cross valid method: FREE R-VALUE / σ(F): 1.52 / Phase error: 33.02 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2737 1516 2.22 %
Rwork0.2395 --
obs0.2403 68285 93.42 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Refinement stepCycle: LAST / Resolution: 3→29.841 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms10177 0 195 0 10372
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.01410627
X-RAY DIFFRACTIONf_angle_d1.72514377
X-RAY DIFFRACTIONf_dihedral_angle_d17.0686276
X-RAY DIFFRACTIONf_chiral_restr0.0731536
X-RAY DIFFRACTIONf_plane_restr0.0091775
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3-3.09680.42431300.39855408X-RAY DIFFRACTION84
3.0968-3.20730.4411260.39015583X-RAY DIFFRACTION86
3.2073-3.33560.37421290.36845662X-RAY DIFFRACTION87
3.3356-3.48720.31981340.32465841X-RAY DIFFRACTION90
3.4872-3.67070.33391320.28176009X-RAY DIFFRACTION93
3.6707-3.90020.32891330.24886131X-RAY DIFFRACTION95
3.9002-4.20060.26411440.21456391X-RAY DIFFRACTION98
4.2006-4.62190.21761470.17816455X-RAY DIFFRACTION100
4.6219-5.28750.20821400.18016482X-RAY DIFFRACTION100
5.2875-6.64960.26681510.22156494X-RAY DIFFRACTION100
6.6496-29.84250.22531500.20356313X-RAY DIFFRACTION96
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
13.08810.21251.51811.93290.24261.0852-0.23440.71720.8037-0.18070.1593-0.0597-0.2077-0.2490.1280.30890.039-0.0640.87160.1330.8513-23.065-70.5515-43.1798
22.9654-3.95820.48626.02071.80218.48510.05761.09620.6927-1.12370.68-1.0298-0.82630.7061-0.56280.5192-0.236-0.28051.33190.47361.334-20.2236-62.653-60.4777
33.8791.2819-1.33091.9761-1.53121.8593-0.81050.64070.8031-0.12110.2737-0.3302-0.23920.25280.52790.4577-0.1504-0.17490.84130.0490.7080.0869-69.2185-44.6895
49.909-0.4752-3.83741.97152.05093.2631.11-1.69440.08040.9838-0.47250.4513-0.76090.0154-0.51220.6071-0.0406-0.15060.9809-0.1120.9813-12.376-63.0899-28.5037
58.74025.10578.34214.90534.82737.8658-0.11670.8451-0.06060.06210.0004-0.4621-0.21420.09750.24390.413-0.0475-0.05530.96860.00180.3646-17.1671-87.2574-41.2455
60.74451.28160.624.6340.83642.82150.04160.42441.0346-0.30910.00730.437-0.4871-0.85580.04510.375-0.0718-0.09311.29070.41261.165-38.5536-71.3058-55.497
72.5066-0.1621-0.24384.6272-0.031.1477-0.06090.44080.8844-0.07280.23440.3519-0.0128-0.5636-0.02540.077-0.2234-0.33791.29330.31570.6838-30.8609-76.7272-54.82
82.17751.86290.58472.21872.20954.8347-0.1061-0.23371.1775-0.1502-0.2620.8452-0.9423-1.04370.2860.5571-0.12780.00661.16770.23161.6566-35.1243-57.1174-50.4905
93.7246-0.9481-0.44431.0074-0.59670.8957-0.33710.16651.1871-0.22890.56470.7526-0.2878-0.0949-0.03880.4158-0.1748-0.25681.49090.21391.0428-48.4228-78.9185-52.0013
103.46421.0012-3.0735.0399-1.11763.5357-0.3871.11260.9977-0.02240.4123-0.1688-0.1481-0.07190.05050.5489-0.0695-0.1781.3560.20851.3727-65.748-68.9446-48.6891
112.28410.1431-0.20920.1098-0.18350.2557-0.1090.89650.50070.1615-0.23230.6622-0.24760.01420.33090.3469-0.3847-0.38852.24960.68421.6875-55.6435-71.2292-61.6806
122.09470.11820.79030.3193-0.84482.79930.46921.136-0.7326-0.51870.35830.03830.8560.2075-0.54220.5829-0.4297-0.47661.79690.52921.1855-43.0354-84.8462-66.9497
131.60241.23982.13431.81662.52827.0971-0.2839-0.4537-0.22480.57760.34110.4858-0.2973-0.4406-0.0394-0.1359-0.12190.96371.2017-0.0999-0.2953-33.7572-91.8164-28.9641
143.5746-0.59071.76793.1913-1.33644.0391-0.02020.0982-1.0458-0.19260.2578-0.03960.67050.1535-0.250.6072-0.08340.24640.7733-0.12880.7003-25.2653-119.651-36.2535
153.4801-1.56551.41263.6173-1.71262.7183-0.0545-0.0999-0.7094-0.0040.31490.3310.1326-0.0594-0.20240.469-0.11230.21890.7081-0.10920.5678-31.419-112.795-34.1732
164.6901-0.5783.14245.6537-4.22852.0059-0.17380.3209-0.95050.13370.5381-1.01780.93331.0512-0.31950.63880.11380.04610.8239-0.11221.0402-17.4854-123.7933-23.5993
172.6299-0.7031-0.4021.68120.25053.75360.01880.2796-0.6982-0.12610.4326-0.54391.80230.7157-0.15840.241-0.40670.25590.8616-0.34641.0439-19.4994-116.976-47.7447
186.68040.60012.04024.45960.02377.8155-0.42431.0706-0.3133-0.48040.2552-0.86141.16521.620.14431.26580.13240.2611.5451-0.44771.2414-3.3861-125.6152-53.2013
195.9694-2.0199-2.0965.49392.17816.73790.12711.1294-1.309-0.29440.02070.38421.1895-0.47930.07761.1863-0.49020.08681.0573-0.42360.9025-26.918-125.9083-50.2852
204.13484.01535.45814.86595.04558.0428-0.34760.1111-1.0183-0.19860.616-0.21490.67070.0399-0.20130.5063-0.08340.22381.177-0.30690.3756-20.9109-99.1461-42.6124
212.87031.92370.03492.4949-0.78292.11090.0659-0.7645-0.70110.8026-0.11650.74650.3961-0.73850.14650.67230.10720.40261.14740.21740.8249-31.119-109.6761-14.7108
223.71391.532-0.16667.7385-0.74315.2979-0.4667-0.3786-0.27140.58050.11410.2585-0.1127-0.12570.31960.44280.10120.04380.79940.01330.2602-21.3283-105.0752-21.1253
234.39194.28480.01299.7644-0.96312.3421-0.4419-0.3619-0.6226-0.11170.08570.76290.6378-0.48170.25650.6969-0.03470.32430.86370.15630.5677-31.8538-110.5452-20.6614
249.63293.291-8.41162.4909-3.35557.8002-0.4741-0.3022-0.75630.51670.23610.89590.0744-0.30780.07950.64450.28730.26081.40480.22950.8612-36.8823-97.5716-7.3589
250.3883-0.4210.10480.4513-0.10650.02620.08820.8575-0.5180.13280.23131.8694-0.0209-1.9756-0.24550.97640.1080.65882.60990.0071.907-54.8711-101.474-4.2518
268.1254-4.81934.81434.71110.67432.0074-0.7628-1.0660.21320.8760.59430.61940.1188-0.92360.1371.46320.16570.68371.67430.04490.9162-45.4887-96.35274.2442
275.7193-1.4245-7.56510.37571.88232.00520.0123-2.0944-0.1061.35080.43450.81170.76690.39-0.2021.62890.06680.46661.38540.09520.8811-30.0072-102.3553-1.0511
283.9732-1.7171-1.51710.88121.09531.97050.8222-0.8222-0.05390.1273-0.173-0.4638-0.62491.3209-0.55181.31250.21940.04891.4289-0.09580.595-17.636-95.8738-6.6599
296.1799-2.5333-0.89092.7981-1.18061.5641-0.7756-0.73390.34360.1208-0.007-0.024-0.2679-0.27380.71140.58730.1677-0.08612.0744-0.12191.1204-81.8106-82.3509-39.2559
305.72980.4061-1.35263.50340.14911.2326-0.2058-1.29270.29560.42380.2333-0.51220.0554-0.012-0.05890.49010.25080.1812.56190.35631.2812-70.069-88.8583-21.4328
311.238-2.1967-1.07314.12971.20553.235-0.1149-0.3126-0.3986-0.04290.1930.7931-0.0894-0.2304-0.00870.38070.31590.35682.59350.42611.0539-74.2984-95.9876-32.5082
324.4424-0.0260.26721.36580.36591.8958-0.7508-1.319-0.668-0.01920.55830.54420.6461-0.13550.5586-1.04510.5060.81762.57680.30181.076-80.0958-90.3482-32.1278
333.1285-0.1798-0.42051.43650.43892.5097-0.5815-1.70410.02640.40240.2482-0.19650.00270.12160.31810.6560.21050.00862.2847-0.00531.2207-74.4769-81.8441-27.354
346.14530.86343.69922.4534-0.0052.4373-0.9736-1.224-0.55331.05580.38650.79210.25840.03190.5950.88970.17570.31832.26540.25681.1268-57.5878-88.9685-33.0678
354.08770.6924-2.35227.50147.04232.0065-0.8421-1.07661.0791-0.1077-0.00950.4211-0.2234-0.15490.82890.82960.3901-0.10861.81540.09661.0128-44.7684-76.9809-28.0302
362.6796-0.0883-0.3826-0.00790.02390.0339-0.1503-1.2574-0.790.45610.32350.3005-0.1753-0.0137-0.13950.63770.65060.4252.7380.39411.4783-50.5504-92.2124-25.0519
377.9590.6444-0.56031.0246-1.02431.03310.6461.0261-2.8382-0.03690.19580.55010.4788-0.4749-0.5670.98180.40040.51291.89370.28682.1916-66.9944-104.7014-33.1699
380.75850.24860.46626.60241.51940.5743-0.0366-0.20990.06280.0719-0.04720.0767-0.0478-0.05410.05860.46830.0434-0.14651.86620.74981.5903-78.2682-66.101-56.952
391.8859-4.38061.18661.9998-2.29311.358-0.51332.090.6478-0.31830.5903-0.0564-0.16030.6771-0.16310.463-0.24050.10522.3609-0.13291.1335-74.5666-84.3581-56.8051
402.53380.9284-1.32912.99321.00511.52210.1440.0167-0.0439-0.2528-0.26860.32150.07490.30830.00450.20570.1392-0.06571.9916-0.65141.0557-86.4718-91.869-71.4525
412.4559-3.5019-0.30524.98360.4350.036-0.2603-0.1449-0.1665-0.49830.17230.0298-0.17790.82470.1090.9512-0.21220.16641.9219-0.56431.3718-96.6703-92.0323-86.248
422.00176.57162.49578.84922.2021.02320.3090.0232-0.1894-0.2655-0.4758-1.04290.33480.22950.05280.7292-0.36410.34142.124-0.16550.947-76.6703-93.6307-78.8842
437.45038.24440.88899.11110.98690.1046-1.42110.05830.0045-2.22370.7928-2.2253-1.81351.27050.51690.92250.080.63742.0674-0.08941.3433-66.8329-94.8876-70.1415
449.4742.91663.97463.5343-2.01275.6140.3085-0.42480.513-0.15890.37620.0236-0.36770.6932-0.57780.6250.17670.14732.0429-0.04681.2108-68.4553-89.9643-63.8643
452.2761-0.5958-0.35862.0135-0.71190.3746-0.2333-0.77770.780.0238-0.0156-0.65550.21640.5582-0.21760.3968-0.11670.57142.316-0.36831.1793-77.7784-84.6844-70.0523
461.99881.99851.99951.99881.99992.00050.1293-0.5117-0.82660.2999-0.0017-0.26431.04870.0964-0.08020.8286-0.06250.38521.7038-0.26720.7059-78.0428-97.3054-57.4122
471.928-3.08731.64374.9288-2.62561.3980.0873-0.45270.00550.44770.163-0.2997-0.13390.0944-0.07420.8985-0.24520.73041.6597-0.44431.3936-78.855-104.5966-60.985
480.0453-0.0603-0.09940.06130.13330.22920.03590.2823-0.2985-0.33330.0661-0.06540.2673-0.1041-0.21150.55840.24120.41461.7534-0.54951.751-88.2227-106.3201-68.7234
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 5 through 116 )
2X-RAY DIFFRACTION2chain 'A' and (resid 117 through 129 )
3X-RAY DIFFRACTION3chain 'A' and (resid 130 through 215 )
4X-RAY DIFFRACTION4chain 'A' and (resid 216 through 233 )
5X-RAY DIFFRACTION5chain 'B' and (resid 5 through 22 )
6X-RAY DIFFRACTION6chain 'B' and (resid 23 through 74 )
7X-RAY DIFFRACTION7chain 'B' and (resid 75 through 116 )
8X-RAY DIFFRACTION8chain 'B' and (resid 117 through 129 )
9X-RAY DIFFRACTION9chain 'B' and (resid 130 through 169 )
10X-RAY DIFFRACTION10chain 'B' and (resid 170 through 195 )
11X-RAY DIFFRACTION11chain 'B' and (resid 196 through 216 )
12X-RAY DIFFRACTION12chain 'B' and (resid 217 through 233 )
13X-RAY DIFFRACTION13chain 'C' and (resid 5 through 22 )
14X-RAY DIFFRACTION14chain 'C' and (resid 23 through 74 )
15X-RAY DIFFRACTION15chain 'C' and (resid 75 through 116 )
16X-RAY DIFFRACTION16chain 'C' and (resid 117 through 129 )
17X-RAY DIFFRACTION17chain 'C' and (resid 130 through 171 )
18X-RAY DIFFRACTION18chain 'C' and (resid 172 through 195 )
19X-RAY DIFFRACTION19chain 'C' and (resid 196 through 233 )
20X-RAY DIFFRACTION20chain 'D' and (resid 5 through 22 )
21X-RAY DIFFRACTION21chain 'D' and (resid 23 through 64 )
22X-RAY DIFFRACTION22chain 'D' and (resid 65 through 116 )
23X-RAY DIFFRACTION23chain 'D' and (resid 117 through 139 )
24X-RAY DIFFRACTION24chain 'D' and (resid 140 through 171 )
25X-RAY DIFFRACTION25chain 'D' and (resid 172 through 182 )
26X-RAY DIFFRACTION26chain 'D' and (resid 183 through 201 )
27X-RAY DIFFRACTION27chain 'D' and (resid 202 through 216 )
28X-RAY DIFFRACTION28chain 'D' and (resid 217 through 233 )
29X-RAY DIFFRACTION29chain 'E' and (resid 5 through 39 )
30X-RAY DIFFRACTION30chain 'E' and (resid 40 through 64 )
31X-RAY DIFFRACTION31chain 'E' and (resid 65 through 77 )
32X-RAY DIFFRACTION32chain 'E' and (resid 78 through 116 )
33X-RAY DIFFRACTION33chain 'E' and (resid 117 through 139 )
34X-RAY DIFFRACTION34chain 'E' and (resid 140 through 169 )
35X-RAY DIFFRACTION35chain 'E' and (resid 170 through 186 )
36X-RAY DIFFRACTION36chain 'E' and (resid 187 through 216 )
37X-RAY DIFFRACTION37chain 'E' and (resid 217 through 233 )
38X-RAY DIFFRACTION38chain 'F' and (resid 9 through 12 )
39X-RAY DIFFRACTION39chain 'F' and (resid 13 through 22 )
40X-RAY DIFFRACTION40chain 'F' and (resid 23 through 36 )
41X-RAY DIFFRACTION41chain 'F' and (resid 37 through 41 )
42X-RAY DIFFRACTION42chain 'F' and (resid 42 through 65 )
43X-RAY DIFFRACTION43chain 'F' and (resid 66 through 70 )
44X-RAY DIFFRACTION44chain 'F' and (resid 83 through 91 )
45X-RAY DIFFRACTION45chain 'F' and (resid 92 through 134 )
46X-RAY DIFFRACTION46chain 'F' and (resid 135 through 139 )
47X-RAY DIFFRACTION47chain 'F' and (resid 140 through 148 )
48X-RAY DIFFRACTION48chain 'F' and (resid 149 through 154 )

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