+Open data
-Basic information
Entry | Database: SASBDB / ID: SASDD42 |
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Sample | Synechocystis fluorescence recovery protein SynFRP.8-109
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Function / homology | : / Fluorescence recovery protein / Photoprotection regulator fluorescence recovery protein / plasma membrane-derived thylakoid membrane / Fluorescence recovery protein Function and homology information |
Biological species | Synechocystis sp. (strain PCC 6803 / Kazusa) (bacteria) |
Citation | Date: 2018 Mar Title: Functional interaction of low-homology FRPs from different cyanobacteria with Synechocystis OCP Authors: Slonimskiy Y / Maksimov E / Lukashev E / Moldenhauer M / Jeffries C / Svergun D / Friedrich T |
Contact author |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Models
Model #1705 | Type: dummy / Software: (2.3i) / Radius of dummy atoms: 1.90 A / Symmetry: P2 / Chi-square value: 1.15 / P-value: 0.735192 Search similar-shape structures of this assembly by Omokage search (details) |
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Model #1706 | Type: atomic / Software: (07) / Radius of dummy atoms: 1.90 A / Symmetry: P2 Comment: Model fitted using CRYSOL post SASREF refinement (harmonics = 25; number of points = 256; constant) Chi-square value: 1.387 / P-value: 0.000320 Search similar-shape structures of this assembly by Omokage search (details) |
-Sample
Sample | Name: Synechocystis fluorescence recovery protein SynFRP.8-109 Specimen concentration: 5 mg/ml |
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Buffer | Name: 20 mM Tris-HCl, 150 mM NaCl, 0.1 mM EDTA, 2 mM dithiothreitol, 3 % v/v glycerol pH: 7.6 |
Entity #915 | Name: FRP / Type: protein / Description: Fluorescence recovery protein / Formula weight: 11.625 / Num. of mol.: 2 / Source: Synechocystis sp. (strain PCC 6803 / Kazusa) / References: UniProt: P74103 Sequence: PWSQAETQSA HALFRKAYQR ELDGLLATVQ AQASQITQID DLWKLHDFLS AKRHEIDGKY DDRQSVIIFV FAQLLKEGLV QAEELTFLAA DKQSKIKALA RL |
-Experimental information
Beam | Instrument name: PETRA III P12 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotron / Wavelength: 0.124 Å | |||||||||||||||||||||||||||||||||
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Detector | Name: Pilatus 2M | |||||||||||||||||||||||||||||||||
Scan | Title: Synechocystis fluorescence recovery protein FRP.8-109 Measurement date: Sep 1, 2017 / Storage temperature: 10 °C / Cell temperature: 10 °C / Exposure time: 1 sec. / Number of frames: 3600 / Unit: 1/nm /
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Distance distribution function P(R) | Sofotware P(R): GNOM 5.0 / Number of points: 1357 /
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Result | Type of curve: single_conc Comments: The experimental MW quoted above is derived from the average value obtained from MALLS (28 kda), MW from Porod volume (23 kDa), MW from the volume of correlation (23 kDa) and from the ...Comments: The experimental MW quoted above is derived from the average value obtained from MALLS (28 kda), MW from Porod volume (23 kDa), MW from the volume of correlation (23 kDa) and from the method of Fischer et al, SAXSMOW (30 kDa). The hydrodynamic radius obtained from DLS is 2.86 nm. Additional GASBOR models, with spatial alignments and the NSD estimate can be located in the full entry zip archive.
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