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- SASDD42: Synechocystis fluorescence recovery protein SynFRP.8-109 -

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Basic information

Entry
Database: SASBDB / ID: SASDD42
SampleSynechocystis fluorescence recovery protein SynFRP.8-109
  • Fluorescence recovery protein (protein), FRP, Synechocystis sp. (strain PCC 6803 / Kazusa)
Function / homologyFluorescence recovery protein / Photoprotection regulator fluorescence recovery protein / plasma membrane-derived thylakoid membrane / Fluorescence recovery protein
Function and homology information
Biological speciesSynechocystis sp. (strain PCC 6803 / Kazusa) (bacteria)
CitationDate: 2018 Mar
Title: Functional interaction of low-homology FRPs from different cyanobacteria with Synechocystis OCP
Authors: Slonimskiy Y / Maksimov E / Lukashev E / Moldenhauer M / Jeffries C / Svergun D / Friedrich T
Contact author
  • Cy M Jeffries (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

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Structure visualization

Structure viewerMolecule:
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Models

Model #1705
Type: dummy / Software: (2.3i) / Radius of dummy atoms: 1.90 A / Symmetry: P2 / Chi-square value: 1.15 / P-value: 0.735192
Search similar-shape structures of this assembly by Omokage search (details)
Model #1706
Type: atomic / Software: (07) / Radius of dummy atoms: 1.90 A / Symmetry: P2
Comment: Model fitted using CRYSOL post SASREF refinement (harmonics = 25; number of points = 256; constant)
Chi-square value: 1.387 / P-value: 0.000320
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Synechocystis fluorescence recovery protein SynFRP.8-109
Specimen concentration: 5 mg/ml
BufferName: 20 mM Tris-HCl, 150 mM NaCl, 0.1 mM EDTA, 2 mM dithiothreitol, 3 % v/v glycerol
pH: 7.6
Entity #915Name: FRP / Type: protein / Description: Fluorescence recovery protein / Formula weight: 11.625 / Num. of mol.: 2 / Source: Synechocystis sp. (strain PCC 6803 / Kazusa) / References: UniProt: P74103
Sequence:
PWSQAETQSA HALFRKAYQR ELDGLLATVQ AQASQITQID DLWKLHDFLS AKRHEIDGKY DDRQSVIIFV FAQLLKEGLV QAEELTFLAA DKQSKIKALA RL

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Experimental information

BeamInstrument name: PETRA III P12 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.124 Å
DetectorName: Pilatus 2M
Scan
Title: Synechocystis fluorescence recovery protein FRP.8-109
Measurement date: Sep 1, 2017 / Storage temperature: 10 °C / Cell temperature: 10 °C / Exposure time: 1 sec. / Number of frames: 3600 / Unit: 1/nm /
MinMax
Q0.127 3.8691
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 1357 /
MinMax
Q0.127035 3.8691
P(R) point1 1357
R0 10.5
Result
Type of curve: single_conc
Comments: The experimental MW quoted above is derived from the average value obtained from MALLS (28 kda), MW from Porod volume (23 kDa), MW from the volume of correlation (23 kDa) and from the ...Comments: The experimental MW quoted above is derived from the average value obtained from MALLS (28 kda), MW from Porod volume (23 kDa), MW from the volume of correlation (23 kDa) and from the method of Fischer et al, SAXSMOW (30 kDa). The hydrodynamic radius obtained from DLS is 2.86 nm. Additional GASBOR models, with spatial alignments and the NSD estimate can be located in the full entry zip archive.
ExperimentalStandardPorod
MW26 kDa26 kDa23 kDa
Volume--36 nm3

P(R)GuinierGuinier error
Forward scattering, I00.01667 0.01643 3.0E-5
Radius of gyration, Rg2.909 nm2.76 nm0.02

MinMax
D-10.5
Guinier point1 125

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