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Open data
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Basic information
Entry | Database: SASBDB / ID: SASDCU2 |
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![]() | Bromodomain testis-specific protein (BRDT) tandem bromodomains
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Function / homology | ![]() sperm DNA condensation / male meiotic nuclear division / male meiosis I / regulation of RNA splicing / histone reader activity / RNA splicing / lysine-acetylated histone binding / mRNA processing / histone binding / transcription coactivator activity ...sperm DNA condensation / male meiotic nuclear division / male meiosis I / regulation of RNA splicing / histone reader activity / RNA splicing / lysine-acetylated histone binding / mRNA processing / histone binding / transcription coactivator activity / chromatin remodeling / regulation of DNA-templated transcription / positive regulation of gene expression / nucleus Similarity search - Function |
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![]() | ![]() Title: Interactome Rewiring Following Pharmacological Targeting of BET Bromodomains. Authors: Jean-Philippe Lambert / Sarah Picaud / Takao Fujisawa / Huayun Hou / Pavel Savitsky / Liis Uusküla-Reimand / Gagan D Gupta / Hala Abdouni / Zhen-Yuan Lin / Monika Tucholska / James D R ...Authors: Jean-Philippe Lambert / Sarah Picaud / Takao Fujisawa / Huayun Hou / Pavel Savitsky / Liis Uusküla-Reimand / Gagan D Gupta / Hala Abdouni / Zhen-Yuan Lin / Monika Tucholska / James D R Knight / Beatriz Gonzalez-Badillo / Nicole St-Denis / Joseph A Newman / Manuel Stucki / Laurence Pelletier / Nuno Bandeira / Michael D Wilson / Panagis Filippakopoulos / Anne-Claude Gingras / ![]() ![]() ![]() ![]() ![]() Abstract: Targeting bromodomains (BRDs) of the bromo-and-extra-terminal (BET) family offers opportunities for therapeutic intervention in cancer and other diseases. Here, we profile the interactomes of BRD2, ...Targeting bromodomains (BRDs) of the bromo-and-extra-terminal (BET) family offers opportunities for therapeutic intervention in cancer and other diseases. Here, we profile the interactomes of BRD2, BRD3, BRD4, and BRDT following treatment with the pan-BET BRD inhibitor JQ1, revealing broad rewiring of the interaction landscape, with three distinct classes of behavior for the 603 unique interactors identified. A group of proteins associate in a JQ1-sensitive manner with BET BRDs through canonical and new binding modes, while two classes of extra-terminal (ET)-domain binding motifs mediate acetylation-independent interactions. Last, we identify an unexpected increase in several interactions following JQ1 treatment that define negative functions for BRD3 in the regulation of rRNA synthesis and potentially RNAPII-dependent gene expression that result in decreased cell proliferation. Together, our data highlight the contributions of BET protein modules to their interactomes allowing for a better understanding of pharmacological rewiring in response to JQ1. |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
-Models
Model #1187 | ![]() Type: dummy / Radius of dummy atoms: 3.75 A / Chi-square value: 1.270 / P-value: 0.000410 ![]() |
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Sample
![]() | Name: Bromodomain testis-specific protein (BRDT) tandem bromodomains Specimen concentration: 13 mg/ml |
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Buffer | Name: 20 mM HEPES, 150 mM NaCl, 2% glycerol, 0.5 mM TCEP / pH: 7.5 |
Entity #620 | Name: BRDT / Type: protein / Description: Bromodomain testis-specific protein / Formula weight: 42.59 / Num. of mol.: 1 / Source: Homo sapiens / References: UniProt: Q58F21 Sequence: SMAIIVNPPP PEYINTKKNG RLTNQLQYLQ KVVLKDLWKH SFSWPFQRPV DAVKLQLPDY YTIIKNPMDL NTIKKRLENK YYAKASECIE DFNTMFSNCY LYNKPGDDIV LMAQALEKLF MQKLSQMPQE EQVVGVKERI KKGTQQNIAV SSAKEKSSPS ATEKVFKQQE ...Sequence: SMAIIVNPPP PEYINTKKNG RLTNQLQYLQ KVVLKDLWKH SFSWPFQRPV DAVKLQLPDY YTIIKNPMDL NTIKKRLENK YYAKASECIE DFNTMFSNCY LYNKPGDDIV LMAQALEKLF MQKLSQMPQE EQVVGVKERI KKGTQQNIAV SSAKEKSSPS ATEKVFKQQE IPSVFPKTSI SPLNVVQGAS VNSSSQTAAQ VTKGVKRKAD TTTPATSAVK ASSEFSPTFT EKSVALPPIK ENMPKNVLPD SQQQYNVVKT VKVTEQLRHC SEILKEMLAK KHFSYAWPFY NPVDVNALGL HNYYDVVKNP MDLGTIKEKM DNQEYKDAYK FAADVRLMFM NCYKYNPPDH EVVTMARMLQ DVFETHFSKI PIE |
-Experimental information
Beam | Instrument name: Diamond Light Source B21 / City: Oxfordshire / 国: UK ![]() | |||||||||||||||||||||||||||||||||||||||
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Detector | Name: Pilatus 2M | |||||||||||||||||||||||||||||||||||||||
Scan | Measurement date: Jan 13, 2017 / Storage temperature: 10 °C / Cell temperature: 10 °C / Exposure time: 10 sec. / Number of frames: 12 / Unit: 1/A /
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Distance distribution function P(R) |
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Result |
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