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基本情報
登録情報 | ![]() |
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![]() | Ammonium transporter Ks-Amt5 (Batch)
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機能・相同性 | ![]() ammonium homeostasis / ammonium channel activity / phosphorelay sensor kinase activity / plasma membrane 類似検索 - 分子機能 |
生物種 | ![]() |
![]() | ![]() タイトル: Signaling ammonium across membranes through an ammonium sensor histidine kinase. 著者: Tobias Pflüger / Camila F Hernández / Philipp Lewe / Fabian Frank / Haydyn Mertens / Dmitri Svergun / Manfred W Baumstark / Vladimir Y Lunin / Mike S M Jetten / Susana L A Andrade / ![]() ![]() ![]() 要旨: Sensing and uptake of external ammonium is essential for anaerobic ammonium-oxidizing (anammox) bacteria, and is typically the domain of the ubiquitous Amt/Rh ammonium transporters. Here, we report ...Sensing and uptake of external ammonium is essential for anaerobic ammonium-oxidizing (anammox) bacteria, and is typically the domain of the ubiquitous Amt/Rh ammonium transporters. Here, we report on the structure and function of an ammonium sensor/transducer from the anammox bacterium "Candidatus Kuenenia stuttgartiensis" that combines a membrane-integral ammonium transporter domain with a fused histidine kinase. It contains a high-affinity ammonium binding site not present in assimilatory Amt proteins. The levels of phosphorylated histidine in the kinase are coupled to the presence of ammonium, as conformational changes during signal recognition by the Amt module are transduced internally to modulate the kinase activity. The structural analysis of this ammonium sensor by X-ray crystallography and small-angle X-ray-scattering reveals a flexible, bipartite system that recruits a large uptake transporter as a sensory module and modulates its functionality to achieve a mechanistic coupling to a kinase domain in order to trigger downstream signaling events. |
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構造の表示
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-モデル
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試料
![]() | 名称: Ammonium transporter Ks-Amt5 (Batch) / Dry vol: 95 / 試料濃度: 1 mg/ml / 濃度測定法: A280 |
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バッファ | 名称: 20 mM Tris/HCl,100 mM NaCl, 5%(w/v) glycerol, 0.09%(w/v) CYMAL-5 pH: 8 |
要素 #909 | 名称: Ks-Amt5 / タイプ: protein / 記述: ammonium sensor/transducer / 分子量: 75.514 / 分子数: 3 / 由来: Candidatus Kuenenia stuttgartiensis / 参照: UniProt: Q1Q357 配列: MENIQININH LWVIMAACMV FLMQLGFTSY ETGFSQSKNA ISIALRNLVD TLISSLVFFS VGFGFMFGKS YMGLIGIDLF FANDLALHPN TLSYSFFFFQ MVFASTAATI LTGAIAERSG FIPNIAGTAF IVAIIYPIFG HWAWGNLFSP DQTGWLKELG FIDFAGATVV ...配列: MENIQININH LWVIMAACMV FLMQLGFTSY ETGFSQSKNA ISIALRNLVD TLISSLVFFS VGFGFMFGKS YMGLIGIDLF FANDLALHPN TLSYSFFFFQ MVFASTAATI LTGAIAERSG FIPNIAGTAF IVAIIYPIFG HWAWGNLFSP DQTGWLKELG FIDFAGATVV HSIGGWFAMA AAIMVGPRID KYNPDGSSNR IGLHNVPLAT LGTFFLWFGW FGFNGGSLLR VSVNIGLVIL NTNMAAASAG VSALIFIYAT RKRIEAGSLF TAILAGLVAI TASSNMVTPV SAVAIGLITG ILAIIAEGFI EKTLKIDDPV SAIAVHGVGG VIGTLCVAIF AQKSYLLAEN GSRMHQLGIQ ALGVIVAFSW SFGLGMLFFL CLKKVKRLRV TPEEEKRGLN VAEYEDVASW LDFIRITRLQ DINTILEKRV QEKTADLQMA NVALEKANRL KSEFLTTMSH ELRTPLNAII GFAEVLRDEI AGSLSKDQKE YVTDIHSSGH HLLDMINNIL DLSKIETGKM HLQYEEFCIE DAINDTLTII NASANNKGIS VHTNIQDNTP LLSADKTKFR QILYNLLSNA VKFTPENGKI TINVFQKDNS LQFEIVDTGI GIKPEDKEKL FEAFHQADAS LTREYEGTGL GLHLTKRLVE LHGGKIWAES TFGKGSTFFF ILPINPVNKL EHHHHHH |
-実験情報
ビーム | 設備名称: PETRA III EMBL P12 / 地域: Hamburg / 国: Germany ![]() | |||||||||||||||||||||||||||||||||||||||||||||||||||
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検出器 | 名称: Pilatus 2M | |||||||||||||||||||||||||||||||||||||||||||||||||||
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距離分布関数 P(R) |
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結果 | コメント: Batch mode Ks-Amt5 sample in 0.09% (w/v) CYMAL-5 detergent. Pooled fractions from offline SEC.
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