+データを開く
-基本情報
登録情報 | データベース: SASBDB / ID: SASDCN9 |
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試料 | Ks-Amt5-HK (apo)
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機能・相同性 | 機能・相同性情報 |
生物種 | Candidatus Kuenenia stuttgartiensis (バクテリア) |
引用 | ジャーナル: Nat Commun / 年: 2018 タイトル: Signaling ammonium across membranes through an ammonium sensor histidine kinase. 著者: Tobias Pflüger / Camila F Hernández / Philipp Lewe / Fabian Frank / Haydyn Mertens / Dmitri Svergun / Manfred W Baumstark / Vladimir Y Lunin / Mike S M Jetten / Susana L A Andrade / 要旨: Sensing and uptake of external ammonium is essential for anaerobic ammonium-oxidizing (anammox) bacteria, and is typically the domain of the ubiquitous Amt/Rh ammonium transporters. Here, we report ...Sensing and uptake of external ammonium is essential for anaerobic ammonium-oxidizing (anammox) bacteria, and is typically the domain of the ubiquitous Amt/Rh ammonium transporters. Here, we report on the structure and function of an ammonium sensor/transducer from the anammox bacterium "Candidatus Kuenenia stuttgartiensis" that combines a membrane-integral ammonium transporter domain with a fused histidine kinase. It contains a high-affinity ammonium binding site not present in assimilatory Amt proteins. The levels of phosphorylated histidine in the kinase are coupled to the presence of ammonium, as conformational changes during signal recognition by the Amt module are transduced internally to modulate the kinase activity. The structural analysis of this ammonium sensor by X-ray crystallography and small-angle X-ray-scattering reveals a flexible, bipartite system that recruits a large uptake transporter as a sensory module and modulates its functionality to achieve a mechanistic coupling to a kinase domain in order to trigger downstream signaling events. |
登録者 |
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-構造の表示
-ダウンロードとリンク
-モデル
-試料
試料 | 名称: Ks-Amt5-HK (apo) / 試料濃度: 1.00-5.00 |
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バッファ | 名称: 20 mM Tris/HCl,100 mM NaCl, 5%(w/v) glycerol / pH: 8 |
要素 #910 | 名称: Ks-Amt5-HK / タイプ: protein / 記述: Ammonium transporter histidine kinase domain / 分子量: 30.031 / 分子数: 1 / 由来: Candidatus Kuenenia stuttgartiensis / 参照: UniProt: Q1Q357 配列: FIRITRLQDI NTILEKRVQE KTADLQMANV ALEKANRLKS EFLTTMSAEL RTPLNAIIGF AEVLRDEIAG SLSKDQKEYV TDIHSSGHHL LDMINNILDL SKIETGKMHL QYEEFCIEDA INDTLTIINA SANNKGISVH TNIQDNTPLL SADKTKFRQI LYNLLSNAVK ...配列: FIRITRLQDI NTILEKRVQE KTADLQMANV ALEKANRLKS EFLTTMSAEL RTPLNAIIGF AEVLRDEIAG SLSKDQKEYV TDIHSSGHHL LDMINNILDL SKIETGKMHL QYEEFCIEDA INDTLTIINA SANNKGISVH TNIQDNTPLL SADKTKFRQI LYNLLSNAVK FTPENGKITI NVFQKDNSLQ FEIVDTGIGI KPEDKEKLFE AFHQADASLT REYEGTGLGL HLTKRLVELH GGKIWAESTF GKGSTFFFIL PINPVNK |
-実験情報
ビーム | 設備名称: PETRA III P12 / 地域: Hamburg / 国: Germany / 線源: X-ray synchrotronシンクロトロン / 波長: 0.124 Å / スペクトロメータ・検出器間距離: 2.7 mm | ||||||||||||||||||||||||||||||||||||||||||
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検出器 | 名称: Pilatus 2M | ||||||||||||||||||||||||||||||||||||||||||
スキャン | タイトル: Ks-Amt5-HK (apo) / 測定日: 2013年3月2日 / 保管温度: 20 °C / セル温度: 20 °C / 照射時間: 0.05 sec. / フレーム数: 20 / 単位: 1/nm /
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距離分布関数 P(R) | ソフトウェア P(R): GNOM 5.0 / ポイント数: 1200 /
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結果 | カーブのタイプ: merged
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