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- SASDCQ9: Ks-Amt5-HK (+10uM ATP) (Ammonium transporter histidine kinase dom... -

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Basic information

Entry
Database: SASBDB / ID: SASDCQ9
SampleKs-Amt5-HK (+10uM ATP)
  • Ammonium transporter histidine kinase domain (protein), Ks-Amt5-HK, Candidatus Kuenenia stuttgartiensis
Function / homology
Function and homology information


ammonium homeostasis / ammonium channel activity / phosphorelay sensor kinase activity / plasma membrane
Similarity search - Function
Ammonium transporter / Ammonium transporter AmtB-like domain / Ammonium Transporter Family / Ammonium/urea transporter / His Kinase A (phospho-acceptor) domain / His Kinase A (phosphoacceptor) domain / Signal transduction histidine kinase, dimerisation/phosphoacceptor domain / Signal transduction histidine kinase, dimerisation/phosphoacceptor domain superfamily / Signal transduction histidine kinase-related protein, C-terminal / Histidine kinase domain ...Ammonium transporter / Ammonium transporter AmtB-like domain / Ammonium Transporter Family / Ammonium/urea transporter / His Kinase A (phospho-acceptor) domain / His Kinase A (phosphoacceptor) domain / Signal transduction histidine kinase, dimerisation/phosphoacceptor domain / Signal transduction histidine kinase, dimerisation/phosphoacceptor domain superfamily / Signal transduction histidine kinase-related protein, C-terminal / Histidine kinase domain / Histidine kinase domain profile. / Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase / Histidine kinase-like ATPases / Histidine kinase/HSP90-like ATPase / Histidine kinase/HSP90-like ATPase superfamily
Similarity search - Domain/homology
Ammonium transporter
Similarity search - Component
Biological speciesCandidatus Kuenenia stuttgartiensis (bacteria)
CitationJournal: Nat Commun / Year: 2018
Title: Signaling ammonium across membranes through an ammonium sensor histidine kinase.
Authors: Tobias Pflüger / Camila F Hernández / Philipp Lewe / Fabian Frank / Haydyn Mertens / Dmitri Svergun / Manfred W Baumstark / Vladimir Y Lunin / Mike S M Jetten / Susana L A Andrade /
Abstract: Sensing and uptake of external ammonium is essential for anaerobic ammonium-oxidizing (anammox) bacteria, and is typically the domain of the ubiquitous Amt/Rh ammonium transporters. Here, we report ...Sensing and uptake of external ammonium is essential for anaerobic ammonium-oxidizing (anammox) bacteria, and is typically the domain of the ubiquitous Amt/Rh ammonium transporters. Here, we report on the structure and function of an ammonium sensor/transducer from the anammox bacterium "Candidatus Kuenenia stuttgartiensis" that combines a membrane-integral ammonium transporter domain with a fused histidine kinase. It contains a high-affinity ammonium binding site not present in assimilatory Amt proteins. The levels of phosphorylated histidine in the kinase are coupled to the presence of ammonium, as conformational changes during signal recognition by the Amt module are transduced internally to modulate the kinase activity. The structural analysis of this ammonium sensor by X-ray crystallography and small-angle X-ray-scattering reveals a flexible, bipartite system that recruits a large uptake transporter as a sensory module and modulates its functionality to achieve a mechanistic coupling to a kinase domain in order to trigger downstream signaling events.
Contact author
  • Haydyn Mertens (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

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Structure visualization

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Models

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Sample

SampleName: Ks-Amt5-HK (+10uM ATP) / Specimen concentration: 1.00-5.00
BufferName: 20 mM Tris/HCl,100 mM NaCl, 5%(w/v) glycerol / pH: 8
Entity #910Name: Ks-Amt5-HK / Type: protein / Description: Ammonium transporter histidine kinase domain / Formula weight: 30.031 / Num. of mol.: 1 / Source: Candidatus Kuenenia stuttgartiensis / References: UniProt: Q1Q357
Sequence: FIRITRLQDI NTILEKRVQE KTADLQMANV ALEKANRLKS EFLTTMSAEL RTPLNAIIGF AEVLRDEIAG SLSKDQKEYV TDIHSSGHHL LDMINNILDL SKIETGKMHL QYEEFCIEDA INDTLTIINA SANNKGISVH TNIQDNTPLL SADKTKFRQI LYNLLSNAVK ...Sequence:
FIRITRLQDI NTILEKRVQE KTADLQMANV ALEKANRLKS EFLTTMSAEL RTPLNAIIGF AEVLRDEIAG SLSKDQKEYV TDIHSSGHHL LDMINNILDL SKIETGKMHL QYEEFCIEDA INDTLTIINA SANNKGISVH TNIQDNTPLL SADKTKFRQI LYNLLSNAVK FTPENGKITI NVFQKDNSLQ FEIVDTGIGI KPEDKEKLFE AFHQADASLT REYEGTGLGL HLTKRLVELH GGKIWAESTF GKGSTFFFIL PINPVNK

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Experimental information

BeamInstrument name: PETRA III P12 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotron / Wavelength: 0.124 Å / Dist. spec. to detc.: 2.7 mm
DetectorName: Pilatus 2M
Scan
Title: Ks-Amt5-HK (+10uM ATP) / Measurement date: Mar 2, 2013 / Storage temperature: 20 °C / Cell temperature: 20 °C / Exposure time: 0.05 sec. / Number of frames: 20 / Unit: 1/nm /
MinMax
Q0.1689 4.423
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 1198 /
MinMax
Q0.174163 3.35148
P(R) point1 1198
R0 7.7
Result
Type of curve: merged
ExperimentalStandardStandard errorPorod
MW28.6 kDa28.6 kDa3 31 kDa
Volume---53 nm3

P(R)P(R) errorGuinierGuinier error
Forward scattering, I01801 6 1795 7
Radius of gyration, Rg2.2 nm0.1 2.2 nm0.1

MinMaxError
D-7.7 0.5
Guinier point3 161 -

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