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TitleSignaling ammonium across membranes through an ammonium sensor histidine kinase.
Journal, issue, pagesNat Commun, Vol. 9, Issue 1, Page 164, Year 2018
Publish dateJan 11, 2018
AuthorsTobias Pflüger / Camila F Hernández / Philipp Lewe / Fabian Frank / Haydyn Mertens / Dmitri Svergun / Manfred W Baumstark / Vladimir Y Lunin / Mike S M Jetten / Susana L A Andrade /
PubMed AbstractSensing and uptake of external ammonium is essential for anaerobic ammonium-oxidizing (anammox) bacteria, and is typically the domain of the ubiquitous Amt/Rh ammonium transporters. Here, we report ...Sensing and uptake of external ammonium is essential for anaerobic ammonium-oxidizing (anammox) bacteria, and is typically the domain of the ubiquitous Amt/Rh ammonium transporters. Here, we report on the structure and function of an ammonium sensor/transducer from the anammox bacterium "Candidatus Kuenenia stuttgartiensis" that combines a membrane-integral ammonium transporter domain with a fused histidine kinase. It contains a high-affinity ammonium binding site not present in assimilatory Amt proteins. The levels of phosphorylated histidine in the kinase are coupled to the presence of ammonium, as conformational changes during signal recognition by the Amt module are transduced internally to modulate the kinase activity. The structural analysis of this ammonium sensor by X-ray crystallography and small-angle X-ray-scattering reveals a flexible, bipartite system that recruits a large uptake transporter as a sensory module and modulates its functionality to achieve a mechanistic coupling to a kinase domain in order to trigger downstream signaling events.
External linksNat Commun / PubMed:29323112 / PubMed Central
MethodsSAS (X-ray synchrotron) / X-ray diffraction
Resolution1.98 Å
Structure data

SASDCM9:
Ammonium transporter Ks-Amt5 (ammonium sensor/transducer, Ks-Amt5)
Method: SAXS/SANS

SASDCN9: Ks-Amt5-HK (apo) (Ammonium transporter histidine kinase domain, Ks-Amt5-HK)
Method: SAXS/SANS

SASDCP9: Ks-Amt5-HK (+10uM APPCP) (Ammonium transporter histidine kinase domain, Ks-Amt5-HK)
Method: SAXS/SANS

SASDCQ9: Ks-Amt5-HK (+10uM ATP) (Ammonium transporter histidine kinase domain, Ks-Amt5-HK)
Method: SAXS/SANS

SASDCS9: Ammonium transporter Ks-Amt5 (Batch) (ammonium sensor/transducer, Ks-Amt5)
Method: SAXS/SANS

PDB-6eu6:
Sensor Amt Protein
Method: X-RAY DIFFRACTION / Resolution: 1.98 Å

Chemicals

ChemComp-MES:
2-(N-MORPHOLINO)-ETHANESULFONIC ACID / pH buffer*YM / MES (buffer)

ChemComp-ZDM:
nonyl 4-O-alpha-D-glucopyranosyl-beta-D-glucopyranoside / detergent*YM

ChemComp-LMT:
DODECYL-BETA-D-MALTOSIDE / detergent*YM

ChemComp-NH4:
AMMONIUM ION / Ammonium

ChemComp-SO4:
SULFATE ION / Sulfate

ChemComp-HOH:
WATER / Water

Source
  • Candidatus kuenenia stuttgartiensis (bacteria)
  • kuenenia stuttgartiensis (bacteria)
KeywordsMEMBRANE PROTEIN / Ammonium transporter / Signalling / histidine kinase

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