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Open data
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Basic information
Entry | Database: SASBDB / ID: SASDCQ2 |
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![]() | 4Ca2+-calmodulin - Xenopus laevis
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Function / homology | ![]() |
Biological species | ![]() |
![]() | ![]() Title: 2017 publication guidelines for structural modelling of small-angle scattering data from biomolecules in solution: an update. Authors: Jill Trewhella / Anthony P Duff / Dominique Durand / Frank Gabel / J Mitchell Guss / Wayne A Hendrickson / Greg L Hura / David A Jacques / Nigel M Kirby / Ann H Kwan / Javier Pérez / Lois ...Authors: Jill Trewhella / Anthony P Duff / Dominique Durand / Frank Gabel / J Mitchell Guss / Wayne A Hendrickson / Greg L Hura / David A Jacques / Nigel M Kirby / Ann H Kwan / Javier Pérez / Lois Pollack / Timothy M Ryan / Andrej Sali / Dina Schneidman-Duhovny / Torsten Schwede / Dmitri I Svergun / Masaaki Sugiyama / John A Tainer / Patrice Vachette / John Westbrook / Andrew E Whitten / ![]() ![]() ![]() ![]() ![]() ![]() ![]() Abstract: In 2012, preliminary guidelines were published addressing sample quality, data acquisition and reduction, presentation of scattering data and validation, and modelling for biomolecular small-angle ...In 2012, preliminary guidelines were published addressing sample quality, data acquisition and reduction, presentation of scattering data and validation, and modelling for biomolecular small-angle scattering (SAS) experiments. Biomolecular SAS has since continued to grow and authors have increasingly adopted the preliminary guidelines. In parallel, integrative/hybrid determination of biomolecular structures is a rapidly growing field that is expanding the scope of structural biology. For SAS to contribute maximally to this field, it is essential to ensure open access to the information required for evaluation of the quality of SAS samples and data, as well as the validity of SAS-based structural models. To this end, the preliminary guidelines for data presentation in a publication are reviewed and updated, and the deposition of data and associated models in a public archive is recommended. These guidelines and recommendations have been prepared in consultation with the members of the International Union of Crystallography (IUCr) Small-Angle Scattering and Journals Commissions, the Worldwide Protein Data Bank (wwPDB) Small-Angle Scattering Validation Task Force and additional experts in the field. |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
-Data source
SASBDB page | ![]() |
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-Related structure data
Related structure data | C: citing same article ( |
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Similar structure data |
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External links
Related items in Molecule of the Month |
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-Models
Model #1176 | ![]() Type: dummy / Software: (on-line) / Radius of dummy atoms: 1.80 A / Symmetry: P1 / Chi-square value: 0.844 / P-value: 0.532600 ![]() |
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Model #1177 | ![]() Type: atomic / Software: (on-line) / Radius of dummy atoms: 1.90 A / Symmetry: P1 Comment: Representative model from 1-state ensemble calculation, flexible residues 77-81 Chi-square value: 0.840129176626 / P-value: 0.314400 ![]() |
Model #1178 | ![]() Type: atomic / Software: (on-line) / Radius of dummy atoms: 1.90 A / Symmetry: P1 Comment: Representative model-0 from 2-state ensemble, flexible residues 77-81 Chi-square value: 0.790816234359 / P-value: 0.787700 ![]() |
Model #1179 | ![]() Type: atomic / Software: (on-line) / Radius of dummy atoms: 1.90 A / Symmetry: P1 Comment: Representative model-1 from 2-state ensemble, flexible residues 77-81 Chi-square value: 0.790816234359 / P-value: 0.787700 ![]() |
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Sample
![]() | Name: 4Ca2+-calmodulin - Xenopus laevis / Specimen concentration: 0.74-1.10 |
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Buffer | Name: 25 mM MOPS, 250 mM NaCl, 50 mM KCl, 2 mM TCEP, 0.1% NaN3 pH: 7.5 Comment: 2 mM TCEP and 0.1% (.01538 M) NaN3 used for radiation protection |
Entity #614 | Name: CaM / Type: protein / Description: Calmodulin / Formula weight: 16.706 / Num. of mol.: 1 / Source: Xenopus laevis / References: UniProt: P62155 Sequence: ADQLTEEQIA EFKEAFSLFD KDGDGTITTK ELGTVMRSLG QNPTEAELQD MINEVDADGN GTIDFPEFLT MMARKMKDTD SEEEIREAFR VFDKDGNGYI SAAELRHVMT NLGEKLTDEE VDEMIREADI DGDGQVNYEE FVQMMTAK |
-Experimental information
Beam | Instrument name: Australian Synchrotron SAXS/WAXS / City: Melbourne / 国: Australia ![]() | ||||||||||||||||||||||||||||||||||||
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Detector | Name: Pilatus 1M / Type: Dectris / Pixsize x: 172 mm | ||||||||||||||||||||||||||||||||||||
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Distance distribution function P(R) |
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Result |
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