+Open data
-Basic information
Entry | Database: SASBDB / ID: SASDAT5 |
---|---|
Sample | NGF
|
Biological species | Mus musculus (house mouse) |
Citation | Journal: J Mol Biol / Year: 2008 Title: Dissecting NGF interactions with TrkA and p75 receptors by structural and functional studies of an anti-NGF neutralizing antibody. Authors: Sonia Covaceuszach / Alberto Cassetta / Petr V Konarev / Stefania Gonfloni / Rainer Rudolph / Dmitri I Svergun / Doriano Lamba / Antonino Cattaneo / Abstract: The anti-nerve growth factor (NGF) monoclonal antibody alphaD11 is a potent antagonist that neutralizes the biological functions of its antigen in vivo. NGF antagonism is expected to be a highly ...The anti-nerve growth factor (NGF) monoclonal antibody alphaD11 is a potent antagonist that neutralizes the biological functions of its antigen in vivo. NGF antagonism is expected to be a highly effective and safe therapeutic approach in many pain states. A comprehensive functional and structural analysis of alphaD11 monoclonal antibody was carried out, showing its ability to neutralize NGF binding to either tropomyosine receptor kinase A (TrkA) or p75 receptors. The 3-D structure of the alphaD11 Fab fragment was solved at 1.7 A resolution. A computational docking model of the alphaD11 Fab-NGF complex, based on epitope mapping using a pool of 44 NGF mutants and experimentally validated by small-angle X-ray scattering, provided the structural basis for identifying the residues involved in alphaD11 Fab binding. The present study pinpoints loop II of NGF to be an important structural determinant for NGF biological activity mediated by TrkA receptor. |
Contact author |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Models
Model #139 | Type: dummy / Software: Dammin / Radius of dummy atoms: 1.90 A / Symmetry: P2 / Chi-square value: 3.101121 Search similar-shape structures of this assembly by Omokage search (details) |
---|
-Sample
Sample | Name: NGF / Sample MW: 50 kDa |
---|---|
Buffer | Name: 10 mM Na-phosphate / pH: 7.8 / Composition: 150 mM NaCl 150.000 mM |
Entity #101 | Name: NGF / Type: protein / Description: NGF / Formula weight: 25 / Num. of mol.: 2 / Source: Mus musculus Sequence: GEFSVCDSVS VWVGDKTTAT DIKGKEVTVL AEVNINNSVF RQYFFETKCR ASNPVESGCR GIDSKHWNSY CTTTHTFVKA LTTDEKQAAW RFIRIDTACV CVLSRKAGEF SVCDSVSVWV GDKTTATDIK GKEVTVLAEV NINNSVFRQY FFETKCRASN PVESGCRGID ...Sequence: GEFSVCDSVS VWVGDKTTAT DIKGKEVTVL AEVNINNSVF RQYFFETKCR ASNPVESGCR GIDSKHWNSY CTTTHTFVKA LTTDEKQAAW RFIRIDTACV CVLSRKAGEF SVCDSVSVWV GDKTTATDIK GKEVTVLAEV NINNSVFRQY FFETKCRASN PVESGCRGID SKHWNSYCTT THTFVKALTT DEKQAAWRFI RIDTACVCVL SRKA |
-Experimental information
Beam | Instrument name: DORIS III X33 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotron | ||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Detector | Name: MAR 345 Image Plate | ||||||||||||||||||
Scan | Title: NGF / Measurement date: Oct 20, 2005 / Storage temperature: 15 °C / Cell temperature: 15 °C / Exposure time: 120 sec. / Number of frames: 2 / Unit: 1/nm /
| ||||||||||||||||||
Distance distribution function P(R) | Sofotware P(R): GNOM 4.5a / Number of points: 512 /
| ||||||||||||||||||
Result | D max: 12 / Type of curve: single_conc /
|