+Open data
-Basic information
Entry | Database: SASBDB / ID: SASDAS5 |
---|---|
Sample | ad11 Fab
|
Biological species | Mus musculus (house mouse) |
Citation | Journal: J Mol Biol / Year: 2008 Title: Dissecting NGF interactions with TrkA and p75 receptors by structural and functional studies of an anti-NGF neutralizing antibody. Authors: Sonia Covaceuszach / Alberto Cassetta / Petr V Konarev / Stefania Gonfloni / Rainer Rudolph / Dmitri I Svergun / Doriano Lamba / Antonino Cattaneo / Abstract: The anti-nerve growth factor (NGF) monoclonal antibody alphaD11 is a potent antagonist that neutralizes the biological functions of its antigen in vivo. NGF antagonism is expected to be a highly ...The anti-nerve growth factor (NGF) monoclonal antibody alphaD11 is a potent antagonist that neutralizes the biological functions of its antigen in vivo. NGF antagonism is expected to be a highly effective and safe therapeutic approach in many pain states. A comprehensive functional and structural analysis of alphaD11 monoclonal antibody was carried out, showing its ability to neutralize NGF binding to either tropomyosine receptor kinase A (TrkA) or p75 receptors. The 3-D structure of the alphaD11 Fab fragment was solved at 1.7 A resolution. A computational docking model of the alphaD11 Fab-NGF complex, based on epitope mapping using a pool of 44 NGF mutants and experimentally validated by small-angle X-ray scattering, provided the structural basis for identifying the residues involved in alphaD11 Fab binding. The present study pinpoints loop II of NGF to be an important structural determinant for NGF biological activity mediated by TrkA receptor. |
Contact author |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Models
Model #138 | Type: dummy / Software: Dammin / Radius of dummy atoms: 1.90 A / Comment: P2 / Chi-square value: 3.182656 Search similar-shape structures of this assembly by Omokage search (details) |
---|
-Sample
Sample | Name: ad11 Fab / Sample MW: 50 kDa |
---|---|
Buffer | Name: 50 mM Na-phosphate / pH: 7 / Composition: ethylenediaminetetraacetic aci 1.000 mM |
Entity #100 | Name: Fab / Type: protein / Description: aD11 Fab / Formula weight: 25 / Num. of mol.: 2 / Source: Mus musculus Sequence: DIQMTQSPAS LSASLGETVT IECRASEDIY NALAWYQQKP GKSPQLLIYN TDTLHTGVPS RFSGSGSGTQ YSLKINSLQS EDVASYFCQH YFGYPRTFGG GTKLELKRAD AAPTVSIFPP SSEQLASGGA SVVCLLNNFY PKDISVKWKI DGSERQNGVL DSVTDQDSKD ...Sequence: DIQMTQSPAS LSASLGETVT IECRASEDIY NALAWYQQKP GKSPQLLIYN TDTLHTGVPS RFSGSGSGTQ YSLKINSLQS EDVASYFCQH YFGYPRTFGG GTKLELKRAD AAPTVSIFPP SSEQLASGGA SVVCLLNNFY PKDISVKWKI DGSERQNGVL DSVTDQDSKD STYSMSSTLT LTKAEYESHN SYTCEVTHKT STSPVVKSFN RGEQVQLKES GPGLVQPSQT LSLTCTVSGF SLTNNNVNWV RQATGRGLEW MGGVWAGGAT DYNSALKSRL TITRDTSKSQ VFLKMHSLQS SDTATYYCAR DGGYSSSTLY AMDAWGQGTT VTVSSASTTA PSVYPLAPGS MVTLGCLVKG YFPEPVTVTW NSGSLASGVH TFPAVLQSGL YTLSSSVTVP ASPWASEAVT CNVAHPASST KVDKKIVPR |
-Experimental information
Beam | Instrument name: DORIS III X33 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotron | ||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Detector | Name: MAR 345 Image Plate | ||||||||||||||||||
Scan | Title: aD11 FAB / Measurement date: Oct 20, 2005 / Storage temperature: 15 °C / Cell temperature: 15 °C / Unit: 1/nm /
| ||||||||||||||||||
Distance distribution function P(R) | Sofotware P(R): GNOM 4.5a / Number of points: 512 /
| ||||||||||||||||||
Result | D max: 8 / Type of curve: single_conc /
|