+Open data
-Basic information
Entry | Database: SASBDB / ID: SASDAP4 |
---|---|
Sample | Chitinase 60 from Moritella marina
|
Function / homology | Function and homology information chitinase / chitinase activity / chitin catabolic process / chitin binding / carbohydrate binding / carbohydrate metabolic process / extracellular region Similarity search - Function |
Biological species | Moritella marina (bacteria) |
Citation | Journal: Acta Crystallogr D Biol Crystallogr / Year: 2014 Title: Crystal structures of substrate-bound chitinase from the psychrophilic bacterium Moritella marina and its structure in solution. Authors: Piotr H Malecki / Constantinos E Vorgias / Maxim V Petoukhov / Dmitri I Svergun / Wojciech Rypniewski / Abstract: The four-domain structure of chitinase 60 from Moritella marina (MmChi60) is outstanding in its complexity. Many glycoside hydrolases, such as chitinases and cellulases, have multi-domain structures, ...The four-domain structure of chitinase 60 from Moritella marina (MmChi60) is outstanding in its complexity. Many glycoside hydrolases, such as chitinases and cellulases, have multi-domain structures, but only a few have been solved. The flexibility of the hinge regions between the domains apparently makes these proteins difficult to crystallize. The analysis of an active-site mutant of MmChi60 in an unliganded form and in complex with the substrates NAG4 and NAG5 revealed significant differences in the substrate-binding site compared with the previously determined complexes of most studied chitinases. A SAXS experiment demonstrated that in addition to the elongated state found in the crystal, the protein can adapt other conformations in solution ranging from fully extended to compact. |
Contact author |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Models
Model #64 | Type: mix / Software: EOM / Radius of dummy atoms: 1.90 A / Chi-square value: 1.447209 Search similar-shape structures of this assembly by Omokage search (details) |
---|---|
Model #65 | Type: mix / Software: EOM / Radius of dummy atoms: 1.90 A / Chi-square value: 1.447209 Search similar-shape structures of this assembly by Omokage search (details) |
Model #66 | Type: mix / Software: EOM / Radius of dummy atoms: 1.90 A Comment: The fit is obtained by the average of the three EOM models Chi-square value: 1.447209 Search similar-shape structures of this assembly by Omokage search (details) |
-Sample
Sample | Name: Chitinase 60 from Moritella marina / Sample MW: 60.79 kDa / Specimen concentration: 0.60-8.80 |
---|---|
Buffer | Name: 20 mM Tris / pH: 8 / Composition: NaCl 200 mM |
Entity #60 | Type: protein / Description: Chitinase 60 / Formula weight: 60.79 / Num. of mol.: 1 / Source: Moritella marina / References: UniProt: B1VBB0 Sequence: MKLKSILSAA IFTGLFSTAG IAGTITSQDD NVVVGYWHNW CDGRGYQGGN APCVELKTVN PQYNVVNISF MKVYDIAEGR IPTFKLDPTI ALSEAEFIAQ IDTLNSQGRS VLIALGGADA HIELTRGDED ALAAEIIRLT DLYGFDGLDI DLEQAAITAK DNQFVIPAAL ...Sequence: MKLKSILSAA IFTGLFSTAG IAGTITSQDD NVVVGYWHNW CDGRGYQGGN APCVELKTVN PQYNVVNISF MKVYDIAEGR IPTFKLDPTI ALSEAEFIAQ IDTLNSQGRS VLIALGGADA HIELTRGDED ALAAEIIRLT DLYGFDGLDI DLEQAAITAK DNQFVIPAAL KMVKEHYRKT GDNFMITMAP EFPYLTANGA YTPYLTELDG YYDFINPQFY NQGGDGLWIE GVGWIAQNND ALKEEFIYYI ADSLINGTRN YHKIPHDKLV FGLPSNIDAA ATGYIQDPQD LYKAFDRLKA QGQPLRGVMT WSVNWDMGTD AANNSYNQQF IKDYGNFIHN QLPPVTDMTP TLSGIVDTRV ELDSHFDPLI GITAKDYQGN DITADVTVSG SVNTNQVGDY LLTYSVSSDD ETTNQPRKIT VYEILPAFTG ITDTTVVIDS EFDPMQGVSA SRPTQGDLTA NITVTGEVDA NVVGVYELTY QLFYGQDNQQ NMTDKRIVTV VTDAVSDDDW QVGSTYVKDD KVTHNGATWT AQWWTKGEEP GTTGEWGVWR |
-Experimental information
Beam | Instrument name: PETRA III P12 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotron / Wavelength: 0.12 Å / Dist. spec. to detc.: 3.1 mm | |||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Detector | Name: Pilatus 2M | |||||||||||||||||||||||||||
Scan | Title: Chitinase 60 / Measurement date: Mar 18, 2013 / Storage temperature: 10 °C / Cell temperature: 10 °C / Exposure time: 1 sec. / Unit: 1/nm /
| |||||||||||||||||||||||||||
Distance distribution function P(R) | Sofotware P(R): GNOM 4.6 / Number of points: 445 /
| |||||||||||||||||||||||||||
Result | Type of curve: single_conc Comments: The fit is obtained by the average of three EOM models
|