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TitleCrystal structures of substrate-bound chitinase from the psychrophilic bacterium Moritella marina and its structure in solution.
Journal, issue, pagesActa Crystallogr D Biol Crystallogr, Vol. 70, Issue Pt 3, Page 676-684, Year 2014
Publish dateFeb 15, 2014
AuthorsPiotr H Malecki / Constantinos E Vorgias / Maxim V Petoukhov / Dmitri I Svergun / Wojciech Rypniewski /
PubMed AbstractThe four-domain structure of chitinase 60 from Moritella marina (MmChi60) is outstanding in its complexity. Many glycoside hydrolases, such as chitinases and cellulases, have multi-domain structures, ...The four-domain structure of chitinase 60 from Moritella marina (MmChi60) is outstanding in its complexity. Many glycoside hydrolases, such as chitinases and cellulases, have multi-domain structures, but only a few have been solved. The flexibility of the hinge regions between the domains apparently makes these proteins difficult to crystallize. The analysis of an active-site mutant of MmChi60 in an unliganded form and in complex with the substrates NAG4 and NAG5 revealed significant differences in the substrate-binding site compared with the previously determined complexes of most studied chitinases. A SAXS experiment demonstrated that in addition to the elongated state found in the crystal, the protein can adapt other conformations in solution ranging from fully extended to compact.
External linksActa Crystallogr D Biol Crystallogr / PubMed:24598737
MethodsSAS (X-ray synchrotron) / X-ray diffraction
Resolution1.481 - 1.639 Å
Structure data

SASDAP4:
Chitinase 60 from Moritella marina (Chitinase 60)
Method: SAXS/SANS

PDB-4mb3:
Crystal structure of E153Q mutant of cold-adapted chitinase from Moritella marina
Method: X-RAY DIFFRACTION / Resolution: 1.55 Å

PDB-4mb4:
Crystal structure of E153Q mutant of cold-adapted chitinase from Moritella complex with Nag4
Method: X-RAY DIFFRACTION / Resolution: 1.481 Å

PDB-4mb5:
Crystal structure of E153Q mutant of cold-adapted chitinase from Moritella complex with Nag5
Method: X-RAY DIFFRACTION / Resolution: 1.639 Å

Chemicals

ChemComp-NA:
Unknown entry

ChemComp-GLY:
GLYCINE / Glycine

ChemComp-MPD:
(4S)-2-METHYL-2,4-PENTANEDIOL / precipitant*YM / 2-Methyl-2,4-pentanediol

ChemComp-IMD:
IMIDAZOLE / Imidazole

ChemComp-CL:
Unknown entry / Chloride

ChemComp-HOH:
WATER / Water

ChemComp-GOL:
GLYCEROL / Glycerol

ChemComp-SO4:
SULFATE ION / Sulfate

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER / Diethylene glycol

Source
  • moritella marina (bacteria)
KeywordsHYDROLASE / TIM-barrel / alpha/beta-barrel Ig-like / Immunoglobulin like domain / ChBD / Chitin binding domain / Nag4 / Chitinase / hydrolaze / low activity mutant / Nag5 / ligand binding

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